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Author : Ellen M. Ball
Publisher : Unknown
Page : 42 pages
File Size : 48,9 Mb
Release : 1974
Category : Identification
ISBN : STANFORD:36105029408635
Author : M.H.V. Van Regenmortel
Publisher : Elsevier
Page : 317 pages
File Size : 52,7 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323156325
Serology and Immunochemistry of Plant Viruses investigates the antigenic properties of plant viruses. It looks at the practical aspects of plant virus serology, along with the molecular basis of viral antigenicity, antigenic determinants in proteins, the structure of antibodies, virus purification, antiserum production, and the theoretical principles and practical implementation of the various serological techniques. It also considers the problems associated with identification and classification of plant viruses. Organized into 10 chapters, this volume begins with an overview of antigens and antigenic determinants before proceeding with a discussion of the immunochemistry of plant viruses, virus-antibody binding, the role of quaternary structure in antigenicity, and the structure of viral antigenic determinants. The reader is also introduced to the methods and principles of purifying plant viruses, preparation of antisera and purification of antibodies, antigen-antibody interaction, immunochemical techniques used with plant viruses, the role of quaternary structure on viral antigenicity, diagnosis of virus diseases, use of serological criteria for measuring the degree of relationship between viruses, and immunochemical studies of plant viruses. The book includes a bibliography with 1,400 references and a list of all the plant viruses that have been studied by serology. This book will be a useful resource for virologists and plant pathologists, as well as for students and research workers in plant virology, plant pathology, microbiology, and general virology.
Author : Moslih Al-Moslih
Publisher : BoD – Books on Demand
Page : 174 pages
File Size : 45,5 Mb
Release : 2012-03-21
Category : Medical
ISBN : 9789535103707
This book explains the concept of serological methods used in laboratory diagnoses of certain bacteria, mycoplasmas, viruses in humans, animals and plants, certain parasitic agents as well as autoimmune disease. The authors present up-to-date information concerning the serological methods in laboratory diagnosis of such infectious diseases. Section one deals with the serological methods for bacteria. Section 2 deals with serological methods in human, animal and plant viruses. Section 3 is concerned with the serological laboratory diagnosis of echinococcus and human toxocariasis agents. The last section deals with serological laboratory methods in the diagnosis of coeliac disease.
Author : R. E. F. Matthews
Publisher : CRC Press
Page : 391 pages
File Size : 42,6 Mb
Release : 2017-12-08
Category : Electronic
ISBN : 1315892251
Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.
Author : R. E. F. Matthews
Publisher : CRC Press
Page : 400 pages
File Size : 46,5 Mb
Release : 1993-08-03
Category : Science
ISBN : UOM:39015029867101
Overview; Host plants in diagnosis; Experimental transmission of viruses in diagnosis; Virus transmission through soil and by soil-inhabiting; Organisms in diagnosis; Inclusions in diagnosing plant virus diseases; Virus purification in relation to diagnosis; Serological procedures; Electron microscopy of in vitro preparations; Nucleic acid hybridization procedures; dsRNA in diagnosis; Diagnostic methods applicable no viroids; Strategies for implicating virus-like pathogens as the caused of diseases of unknown etiology; Complexes of transmission-dependent and helper viruses.
Author : Anonim
Publisher : Unknown
Page : 302 pages
File Size : 53,7 Mb
Release : 1980
Category : Electronic
ISBN : OCLC:916949468
Author : R. Hampton,Ellen M. Ball
Publisher : Amer Phytopathological Society
Page : 389 pages
File Size : 55,5 Mb
Release : 1990
Category : Science
ISBN : 0890541159
Preparative methods; Assay environments: liquid phase; Assay environments: solid phase; New applications or special topics.
Author : P. Narayanasamy
Publisher : CRC Press
Page : 548 pages
File Size : 41,5 Mb
Release : 2001-08-28
Category : Science
ISBN : 0824705912
This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.
Author : F.C. Bawden
Publisher : Daya Books
Page : 360 pages
File Size : 45,6 Mb
Release : 2002-04
Category : Science
ISBN : 8176220647
The Subject Of Plant Viruses And Virus Diseases Are Receiving Increasing Attention At The Hands Of Research Scholars Such As Chemists, Crystallographers, Entomologists, Geneticists, Serologists, Physicists Etc. They Are Able To Bring Fresh Techniques To The Subject. The Book Is An Attempt To Describe And Correlate The Advances That Have Been Made So Far In The Study Of Plant Viruses, And Virus Diseases. During The Past Few Years, Many New Virus Diseases Have Been Described, Some Of Great Economic Importance And Some With Novel Kinds Of Symptoms; Much Has Been Done On The Factors That Affect Spread; And Insect Vectors Have Been Identified In Species Of Insects Previously Not Incriminated. More Viruses Have Been Purified And Their Chemical Constitution Has Been Further Elucidated, And The Advances In Electron Microscopy Have Provided Much Information On Their Morphology. All These Developments Have Been Incorporated In The Book. The Book Is A Treasure For Agriculture And Horticulture Departments And All Those Interested In These Subjects. Contents Chapter 1: Introduction; Definition Of Subject, Economic Importance, Historical, Views On The Nature Of Viruses, Nomenclature, Chapter 2: External Symptoms Of Infected Plants; Range Of Host Plant Reactions, Type Diseases, Virus Strains, Effects Of Environment On Symptoms, Chapter 3: Internal Symptoms Of Infected Plants; Intracellular Inclusions Formed By Tobacco Mosaic Viruses, Possible Relationship Between Tobacco Mosaic Viruses And The Intracellular Inclusions, Intracellular Inclusions Caused By Viruses Other Than Tobacco Mosaic, Internal Changes Other Than Intracellular Inclusions, Chapter 4: Methods Of Transmission; Grafting And The Use Of Dodder, Inoculation With Plant Extracts, Transmission By Insects, Seed Borne Viruses, Soil Borne Viruses, Chapter 5: The Relations Between Viruses And Their Vectors; Viruses Which Persist In Their Insect Vectors, Latent Period And Virus Multiplication In Vectors, Viruses Whose Vectors Soon Lose Infectivity, Effect Of The Number Of Insects In Transmitting, Chapter 6: Mutation, Interference Between Strains, And Recovery From Disease; Kinds Of Variation, Origin Of Variation, Acquired Immunity, Chapter 7: Serological Reactions Of Plant Viruses; Preparation Of Antisera, Methods Of Testing, The Antigenicity Of Plant Viruses, Neutralisation Of Infectivity, Specificity Of Serological Reactions, Effect Of Particle Shape Of Serological Reactions, Chapter 8: Quantitative Methods Of Assaying For Viruses; The Use Of Local Lesions, Starch Iodine Lesions, The Use Of Preciptin Tests, Chapter 9: The Purification Of Viruses; Treatment Of Plants, Tobacco Mosaic And Other Anisometric Viruses, Tomato Bushy Stunt And Other Crystalline Viruses, High Speed Centrifugation, Activity Of Purifie Preparations, Proteins From Healthy Plants, Chapter 10: Chemical And Physical Properties Of Purified Virus Preparations; Elementary Composition, Isolation And Properties Of Nucleic Acid, The Denatured Protein, Specific Volume And Hydration, Precipitation And Isoelectric Points, Chapter 11: The Crystallinity Of Plant Viruses; Optical Properties Of Crystals, Anistropy Of Flow And The Layering Phenomenon, Crystal And Liquid Crystals, Chapter 12: The Estimation Of Particle Sizes; Filtration, Sedimentation And Diffusion, Microscopy And X-Ray Diffraction, Additional Methods, The Aggregation Of Anisometric Viruses, Chapter 13: Types Of Inactivation; Inhibition Of Infectivity, Inactivation Without Lossof Serological Activity, Inactivation And Denaturation, Chapter 14: Taxonomy Of Viruses; Nomenclature And Classification, The Grouping Of Virus Strains, The Formation Of Larger Groups, Chapter 15: Virus Diseases And Host Plant Physiology; Host Plant Metabolism And Susceptibility, Effects Of Viruses On Host Plant Metabolism, The Movement Of Viruses, Chapter 16: Control Measures Against Virus Diseases; Curative Treatments, General Measures, Insect Borne Viruses, Viruses Transmitted Mechanically, Chapter 17: Speculations On The Origins Of Viruses.
Author : Dirk Noordam
Publisher : Bernan Press(PA)
Page : 226 pages
File Size : 51,7 Mb
Release : 1973
Category : Technology & Engineering
ISBN : UOM:39015004581115
Symbols and abbreviations; Occurrence and prevention of undesirable infection; Mechanical inoculation of a host range; Internal symptoms; Multiplication of a virus in hosts; Testing of virus concentrations on local lesion hosts; Dilution end-point determination; Thermal inactivation point determination; Longevity in vitro (aging) determination; Interaction with other viruses; Purification; Spectrophotometry; Serology; Electron microscopy; Dry weight determination; Transmission of viruses by aphids; Transmission of viruses (or mycoplasmas) by leaphoppers; Nematode transmission; Transmission of tobacco necrosis virus by Olpidium brassicae; Seed transmission of viruses; Transmission of viruses by grafting; Transmission of viruses by Cuscuta (dodder); Separation of viruses from mixed infections; Separation of virus strains from virus-infected plants; Mutants; isolation of mutants or strains; Storage of viruses.
Author : Baozhong Meng,Giovanni P. Martelli,Deborah A. Golino,Marc Fuchs
Publisher : Springer
Page : 698 pages
File Size : 50,7 Mb
Release : 2017-07-05
Category : Science
ISBN : 9783319577067
The domestication of grapes dates back five thousand years ago and has spread to nearly all continents. In recent years, grape acreage has increased dramatically in new regions, including the United States of America, Chile, Asia (China and India), and Turkey. A major limiting factor to the sustained production of premium grapes and wines is infections by viruses. The advent of powerful molecular and metagenomics technologies, such as molecular cloning and next generation sequencing, allowed the discovery of new viruses from grapes. To date, grapevine is susceptible to 64 viruses that belong to highly diverse taxonomic groups. The most damaging diseases include: (1) infectious degeneration; (2) leafroll disease complex; and (3) rugose wood complex. Recently, two new disease syndromes have been recognized: Syrah decline and red blotch. Losses due to fanleaf degeneration are estimated at $1 billion annually in France alone. Other diseases including leafroll, rugose wood, Syrah de cline and red blotch can result in total crop loss several years post-infection. This situation is further exacerbated by mixed infections with multiple viruses and other biotic as well as adverse abiotic environmental conditions, such as drought and winter damage, causing even greater destruction. The book builds upon the last handbook (written over twenty years ago) on the part of diagnostics and extensively expands its scope by inclusion of molecular biology aspects of select viruses that are widespread and economically most important. This includes most current information on the biology, transmission, genome replication, transcription, subcellular localization, as well as virus-host interactions. It also touches on several novel areas of scientific inquiry. It also contains suggested directions for future research in the field of grapevine virology.
Author : J. M. Duncan
Publisher : Wiley-Blackwell
Page : 235 pages
File Size : 47,8 Mb
Release : 1992
Category : Science
ISBN : 0632030666
Immunological techniques: Serological methods to detect plant viruses:production and use of monoclonal antibodies; Immunodiagnostic techniques for plant mycoplasma-like organisms; Detection of plant-invading fungi by monoclonal antibodies; Assay methods for mycotoxins using monoclonal antibodies; Techniques for detecting and identifying plant pathogenic bacteria. Nucleic Acid Techniques: Application of double-stranded RNA analysis of plant to detect viruses, virus-like agents, virus satellites and subgenomic viral RNAs; Detection of viroids and viruses by nucleic acid probes; Identification of plant pathogenic bacteria using nucleic acid technology; Use of RFLPs to identify races of fungal pathogens. Coomercial aspects and practical applications: Screening forpathogens and contaminating micro-organisms in micropropagation; Application of rapid techniques to seed health testing prospects and potential; From the research bench to the market place: development of commercial diagnostic kits; Commercialization of serological tests for plant viruses.
Author : K. Subramanya Sastry
Publisher : Springer Science & Business Media
Page : 343 pages
File Size : 46,8 Mb
Release : 2013-01-05
Category : Medical
ISBN : 9788132208136
Seeds provide an efficient means in disseminating plant virus and viroid diseases. The success of modern agriculture depends on pathogen free seed with high yielding character and in turn disease management. There is a serious scientific concern about the transmission of plant viruses sexually through seed and asexually through plant propagules. The present book provides the latest information along with the total list of seed transmitted virus and viroid diseases at global level including, the yield losses, diagnostic techniques, mechanism of seed transmission, epidemiology and virus disease management aspects. Additional information is also provided on the transmission of plant virus and virus-like diseases through vegetative propagules. It is also well known that seed transmitted viruses are introduced into new countries and continents during large-scale traffic movements through infected germplasm and plant propogules. The latest diagnostic molecular techniques in different virus-host combinations along with disease management measures have been included. The book shall be a good reference source and also a text book to the research scientists, teachers, students of plant pathology, agriculture, horticulture, life sciences, green house managers, professional entrepreneurs, persons involved in quarantines and seed companies. This book has several important features of seed transmitted virus diseases and is a good informative source and thus deserves a place in almost all university libraries, seed companies and research organizations.
Author : Kenneth Manley Smith
Publisher : Chapman & Hall
Page : 280 pages
File Size : 52,7 Mb
Release : 1977
Category : Science
ISBN : STANFORD:36105032781416
Introduction; Symptomatology of virus-infected plants; Physiology of plant virus diseases; Isolation and purification of plant viruses; Morphology and ultrastructure of the virus particles; Chemistry of plant viruses; Infection and replication. Assembly of the tobacco mosaic virus particle. Incomplete viruses. Satellite viruses. Viroids.Transmission by vectors; Transmission other than by vectors; Quantitative assay. Virus inhibitors. Virus strains; Serology of fplant viruses; Tissue-and cell-culture of plant viruses; Testing for viruses: indicator plants; Nomenclature and classification. Control; Fungal and algal viruses; Mycoplasma-like organisms; Index of authors; Subject index.
Author : Alangar Ishwara Bhat,Govind Pratap Rao
Publisher : Humana
Page : 552 pages
File Size : 49,8 Mb
Release : 2021-03-20
Category : Science
ISBN : 1071603361
This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.