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Serology and Immunochemistry of Plant Viruses by M.H.V. Van Regenmortel Pdf
Serology and Immunochemistry of Plant Viruses investigates the antigenic properties of plant viruses. It looks at the practical aspects of plant virus serology, along with the molecular basis of viral antigenicity, antigenic determinants in proteins, the structure of antibodies, virus purification, antiserum production, and the theoretical principles and practical implementation of the various serological techniques. It also considers the problems associated with identification and classification of plant viruses. Organized into 10 chapters, this volume begins with an overview of antigens and antigenic determinants before proceeding with a discussion of the immunochemistry of plant viruses, virus-antibody binding, the role of quaternary structure in antigenicity, and the structure of viral antigenic determinants. The reader is also introduced to the methods and principles of purifying plant viruses, preparation of antisera and purification of antibodies, antigen-antibody interaction, immunochemical techniques used with plant viruses, the role of quaternary structure on viral antigenicity, diagnosis of virus diseases, use of serological criteria for measuring the degree of relationship between viruses, and immunochemical studies of plant viruses. The book includes a bibliography with 1,400 references and a list of all the plant viruses that have been studied by serology. This book will be a useful resource for virologists and plant pathologists, as well as for students and research workers in plant virology, plant pathology, microbiology, and general virology.
Fundamentals of Plant Virology by R C Matthews Pdf
Fundamentals of Plant Virology is an introductory student text covering all of modern plant virology. The author, Dr. R.E.F. Matthews, has written this coursebook based on his classic and comprehensive Plant Virology, Third Edition. Four introductory chapters review properties of viruses and cells and techniques used in their study. Five chapters are devoted to current knowledge of all major plant viruses and related pathogens. Seven chapters describe biological properties such as transmission, host response, disease, ecology, control, classification, and evolution of plant viruses. A historical and future overview concludes the text. Fundamentals of Plant Virology is a carefully designed instructional format for a plant virology course. It is also an invaluable resource for students of plant pathology and plant molecular biology. Summarizes knowledge on all aspects of plant virology Condenses all essential material from Plant Virology 3/e Compares basic properties of cells and viruses Outlines principles of gene manipulation technology Discusses serological techniques including monoclonal antibodies Geared to student level course
The original aim of this book was to cover different aspects of the tradi tionally "filamentous" potex-, carla-, poty-, clostero-, and capilloviruses. The title The Filamentous Plant Viruses seemed the only suitable one, but it has led us to discuss also the quite different filamentous viruses of the rice stripe group-recently officially named the tenuivirus group which otherwise, indeed, might not have been conveniently covered in any volume of this series. The question must be asked: What is there new that justifies the presentation of a book of this kind? An outline of the answer may be Among the traditional filamentous viruses, much pro given as follows. gress has been made in elucidating the physical structure of potexvirus particles, and this work serves as an excellent model for discussion of and future experiments on the poty-, carla-, clostero-, and capilloviruses, which have comparable structures, although they are more difficult to manipulate. Work on the structure and strategy of the genomes of poty viruses is, however, relatively advanced and at a very interesting stage. The helper component that assists the aphid transmission of potyviruses has also recently received considerable attention, although the more we know about that, the less seems clear about the aphid transmission of the carlaviruses and closteroviruses, which apparently neither possess nor require a helper component.
Diagnosis of Plant Virus Diseases by R. E. F. Matthews Pdf
Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.
From virus discovery to virology; Viruses as disease incitants; Viruses as contagious agents; Viruses as physico-chemical particles; Serology and electron microscopy; Viruses as packages of genetic information; Order out of chaos; Ecology of viruses; Human interference with viruses.
Characterization of Plant Viruses by Alangar Ishwara Bhat,Govind Pratap Rao Pdf
This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.
Plant Viruses and Virus Diseases by F.C. Bawden Pdf
The Subject Of Plant Viruses And Virus Diseases Are Receiving Increasing Attention At The Hands Of Research Scholars Such As Chemists, Crystallographers, Entomologists, Geneticists, Serologists, Physicists Etc. They Are Able To Bring Fresh Techniques To The Subject. The Book Is An Attempt To Describe And Correlate The Advances That Have Been Made So Far In The Study Of Plant Viruses, And Virus Diseases. During The Past Few Years, Many New Virus Diseases Have Been Described, Some Of Great Economic Importance And Some With Novel Kinds Of Symptoms; Much Has Been Done On The Factors That Affect Spread; And Insect Vectors Have Been Identified In Species Of Insects Previously Not Incriminated. More Viruses Have Been Purified And Their Chemical Constitution Has Been Further Elucidated, And The Advances In Electron Microscopy Have Provided Much Information On Their Morphology. All These Developments Have Been Incorporated In The Book. The Book Is A Treasure For Agriculture And Horticulture Departments And All Those Interested In These Subjects. Contents Chapter 1: Introduction; Definition Of Subject, Economic Importance, Historical, Views On The Nature Of Viruses, Nomenclature, Chapter 2: External Symptoms Of Infected Plants; Range Of Host Plant Reactions, Type Diseases, Virus Strains, Effects Of Environment On Symptoms, Chapter 3: Internal Symptoms Of Infected Plants; Intracellular Inclusions Formed By Tobacco Mosaic Viruses, Possible Relationship Between Tobacco Mosaic Viruses And The Intracellular Inclusions, Intracellular Inclusions Caused By Viruses Other Than Tobacco Mosaic, Internal Changes Other Than Intracellular Inclusions, Chapter 4: Methods Of Transmission; Grafting And The Use Of Dodder, Inoculation With Plant Extracts, Transmission By Insects, Seed Borne Viruses, Soil Borne Viruses, Chapter 5: The Relations Between Viruses And Their Vectors; Viruses Which Persist In Their Insect Vectors, Latent Period And Virus Multiplication In Vectors, Viruses Whose Vectors Soon Lose Infectivity, Effect Of The Number Of Insects In Transmitting, Chapter 6: Mutation, Interference Between Strains, And Recovery From Disease; Kinds Of Variation, Origin Of Variation, Acquired Immunity, Chapter 7: Serological Reactions Of Plant Viruses; Preparation Of Antisera, Methods Of Testing, The Antigenicity Of Plant Viruses, Neutralisation Of Infectivity, Specificity Of Serological Reactions, Effect Of Particle Shape Of Serological Reactions, Chapter 8: Quantitative Methods Of Assaying For Viruses; The Use Of Local Lesions, Starch Iodine Lesions, The Use Of Preciptin Tests, Chapter 9: The Purification Of Viruses; Treatment Of Plants, Tobacco Mosaic And Other Anisometric Viruses, Tomato Bushy Stunt And Other Crystalline Viruses, High Speed Centrifugation, Activity Of Purifie Preparations, Proteins From Healthy Plants, Chapter 10: Chemical And Physical Properties Of Purified Virus Preparations; Elementary Composition, Isolation And Properties Of Nucleic Acid, The Denatured Protein, Specific Volume And Hydration, Precipitation And Isoelectric Points, Chapter 11: The Crystallinity Of Plant Viruses; Optical Properties Of Crystals, Anistropy Of Flow And The Layering Phenomenon, Crystal And Liquid Crystals, Chapter 12: The Estimation Of Particle Sizes; Filtration, Sedimentation And Diffusion, Microscopy And X-Ray Diffraction, Additional Methods, The Aggregation Of Anisometric Viruses, Chapter 13: Types Of Inactivation; Inhibition Of Infectivity, Inactivation Without Lossof Serological Activity, Inactivation And Denaturation, Chapter 14: Taxonomy Of Viruses; Nomenclature And Classification, The Grouping Of Virus Strains, The Formation Of Larger Groups, Chapter 15: Virus Diseases And Host Plant Physiology; Host Plant Metabolism And Susceptibility, Effects Of Viruses On Host Plant Metabolism, The Movement Of Viruses, Chapter 16: Control Measures Against Virus Diseases; Curative Treatments, General Measures, Insect Borne Viruses, Viruses Transmitted Mechanically, Chapter 17: Speculations On The Origins Of Viruses.
Handbook of Plant Virology by Jawaid Khan,Jeanne Dijkstra Pdf
All the information you need on plant viruses in a single volume The Handbook of Plant Virology is a comprehensive guide to the terms and expressions commonly used in the study of plant virology, complete with descriptions of plant virus families down to the generic level. Rather than simply listing terms in alphabetical order, this unique book links each term to related terms within a theme and adds commentary from authors whose specific expertise adds additional dimensions to the topics. The result is an invaluable resource for research workers, educators, and students working in plant virology and pathology, crop protection, molecular biology, and plant breeding. The Handbook of Plant Virology provides enough details and background in the discussion of each topic to present a clear and thorough understanding of terms without the lengthy analysis found in most textbooks. The book’s first section covers: the mechanics of virus classification internal and external symptoms (with color illustrations) isolation and purification genome packaging replication and gene expression detection and identification various methods of virus transmission serology forecasting disease development recombination control strategies economic importance and much more The second section of The Handbook of Plant Virology is devoted to concise descriptions of the 81 genera and 18 families of plant viruses, including: positive-sense, single-stranded RNA viruses, such as Potyviridae, Sequiviridae, and Comoviridae double-stranded RNA viruses, such as Reoviridae and Partitiviridae negative-sense, single-stranded RNA viruses, such as Rhabdoviridae and Bunyaviridae single-stranded DNA viruses, such as Geminiviridae, Pseudoviridae, Metaviridae The Handbook of Plant Virology also includes photos, illustrations, figures, diagrams, and brief, but detailed, bibliographies. The book’s concise mix of information on currently assigned taxonomic families and the genera of plant viruses make it an essential reference tool for practitioners, researchers, educators, and students.
Introduction; Symptomatology of virus-infected plants; Physiology of plant virus diseases; Isolation and purification of plant viruses; Morphology and ultrastructure of the virus particles; Chemistry of plant viruses; Infection and replication. Assembly of the tobacco mosaic virus particle. Incomplete viruses. Satellite viruses. Viroids.Transmission by vectors; Transmission other than by vectors; Quantitative assay. Virus inhibitors. Virus strains; Serology of fplant viruses; Tissue-and cell-culture of plant viruses; Testing for viruses: indicator plants; Nomenclature and classification. Control; Fungal and algal viruses; Mycoplasma-like organisms; Index of authors; Subject index.
Introduction; From virus discovery to virology; Virus as disease incitants; Viruses as contagious agents; Viruses as physico-chemical particles; Serology and electron microscopy; Viruses as packages of genetic information; Order out of chaos; Ecology of viruses; Human interference with viruses.
