Step Wise Protocols For Somatic Embryogenesis Of Important Woody Plants

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Protocol for Somatic Embryogenesis in Woody Plants

Author : Shri Mohan Jain,Pramod K. Gupta
Publisher : Springer Science & Business Media
Page : 580 pages
File Size : 42,8 Mb
Release : 2005-08-26
Category : Science
ISBN : 9781402029851

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Protocol for Somatic Embryogenesis in Woody Plants by Shri Mohan Jain,Pramod K. Gupta Pdf

World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.

Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

Author : Shri Mohan Jain,Pramod Gupta
Publisher : Springer
Page : 323 pages
File Size : 46,7 Mb
Release : 2018-06-11
Category : Science
ISBN : 9783319894836

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Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants by Shri Mohan Jain,Pramod Gupta Pdf

World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.

Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

Author : Shri Mohan Jain,Pramod Gupta
Publisher : Springer
Page : 392 pages
File Size : 46,9 Mb
Release : 2018-05-30
Category : Science
ISBN : 9783319790879

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Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants by Shri Mohan Jain,Pramod Gupta Pdf

World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.

Somatic Embryogenesis

Author : Marco A. Ramírez-Mosqueda
Publisher : Springer Nature
Page : 272 pages
File Size : 45,8 Mb
Release : 2022-08-11
Category : Science
ISBN : 9781071624852

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Somatic Embryogenesis by Marco A. Ramírez-Mosqueda Pdf

This volume provides an overview of methods for plant species, mainly species with high economic value, to achieve in vitro propagation through somatic embryogenesis. Chapters guide readers through somatic embryogenesis protocols, somatic embryos in plants, and new methodologies that are used out to solve in vitro recalcitrance of many species to this morphogenetic route. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Somatic Embryogenesis: Methods and Protocols aims to guide future researcher to achieve success in obtaining and regenerating somatic embryos. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Somatic Embryogenesis in Woody Plants

Author : S. Mohan Jain,Pramod P.K. Gupta,R.J. Newton
Publisher : Springer Science & Business Media
Page : 385 pages
File Size : 40,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401109604

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Somatic Embryogenesis in Woody Plants by S. Mohan Jain,Pramod P.K. Gupta,R.J. Newton Pdf

These books provide an update to progress on somatic embryogenesis in woody plants including both angiosperm and gymnosperm trees. In the past, most of the information on this subject was scattered in proceedings volumes, journals, biotechnology books, etc. It has been difficult for the researchers and students to obtain comprehensive information on this rapidly growing subject from a single source. These books enable readers to get a clear view of this subject on historical, anatomical, physiological, biochemical and molecular aspects, and applications including protoplasts, cryopreservation, manufactured seed (artificial seed), genetic transformation, bioreactors, mutations, and future uses in forest plantations. Each selected woody plant mentioned in the book is briefly introduced first, covering botany and genetics, importance and geographical distribution, breeding problems, and in vitro propagation and problems of each selected woody plant and then is followed by the description on the initiation and maintenance of embryogenic cultures, embryo development and germination, and field trials (if any) of these plants. These books are meant for graduate students and researchers in forestry and horticulture as well as biotechnologists.

Somatic Embryogenesis in Woody Plants

Author : S.M. Jain,Pramod P.K. Gupta,R.J. Newton
Publisher : Springer Science & Business Media
Page : 549 pages
File Size : 43,9 Mb
Release : 2013-04-17
Category : Science
ISBN : 9789401730327

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Somatic Embryogenesis in Woody Plants by S.M. Jain,Pramod P.K. Gupta,R.J. Newton Pdf

The rapid progress made on somatic embryogenesis and its prospects for potential applications in improving woody plants prompted us to edit this book initially in three volumes, and now to add two more volumes. The editors were all convinced that such a treatise was needed and would be extremely useful to researchers and students. This Volume 4 has been divided into three sections and contains 23 chapters. Section A contains eleven chapters covering studies of embryo development and cell biology of white spruce, proliferative somatic embryogenesis in woody species, somatic embryo germination and desiccation tolerance in conifers, performance of conifer somatic seedlings, apoptosis during early somatic embryogenesis, water relation parameters in conifer embryos, image analysis of somatic embryos, somatic embryogenesis in woody legumes, cold storage and cryopreservation, and commercialization of plant somatic embryogenesis. Section B contains six chapters dealing with angiosperm woody plants, such as somatic embryogenesis in myrtaceous plants, Laurus nobilis, Simarouba glauca, Magnolia spp., Juglans cinera, and somatic embryogenesis and evaluation of variability in somatic seedlings of Quercus serrata by RAPD markers. The chapters contained in Section C are focused on somatic embryogenesis in gymnosperms, including Pinus patula, Encephalartos, Picea wilsonii, Pinus banksiana, hybrid firs, and Taxus. All the chapters have been peer-reviewed and revised accordingly to improve their quality.

Somatic Embryogenesis in Woody Plants

Author : S.M. Jain,Pramod P.K. Gupta,R.J. Newton
Publisher : Springer Science & Business Media
Page : 338 pages
File Size : 47,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401147743

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Somatic Embryogenesis in Woody Plants by S.M. Jain,Pramod P.K. Gupta,R.J. Newton Pdf

The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environmental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding could become a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Generally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalum album), Citrus and mango (Mangifera indica), offer a ray of hope for inexpensive clonal propagation for large-scale production of plants or 'emblings' or somatic seedlings; protoplast work; cryopreservation; genetic transformation; and synthetic or artificial or manufactured seed production.

Somatic Embryogenesis in Woody Plants

Author : S. Mohan Jain,P.K. Gupta,R.J. Newton
Publisher : Springer Science & Business Media
Page : 768 pages
File Size : 48,9 Mb
Release : 2000-09-30
Category : Juvenile Nonfiction
ISBN : 0792364198

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Somatic Embryogenesis in Woody Plants by S. Mohan Jain,P.K. Gupta,R.J. Newton Pdf

The rapid progress on somatic embryogenesis and its prospects for potential application to improving woody plants prompted us to edit this book initially in three volumes, and now an additional three more volumes. We were all convinced that such a treatise was needed and would be extremely useful to researchers and students. This volume 6 is dedicated to Prof. Harry Waris, Helsinki, Finland, who did pioneer work on somatic embryogenesis during the time when Prof. Steward and others were actively engaged in this area. His former student Prof. Liisa Simols, University of Helsinki, Finland, has written a dedication `Harry Waris, a pioneer in somatic embryogenesis' to her teacher Prof. Waris. This volume is divided into three sections and contains a total of 26 chapters. Section A comprises seven chapters covering topics such as: Historical insights into some contemporary problems in somatic embryogenesis (SE); Thin cell layer for somatic embryogenesis induction in woody trees; SE in tropical fruit and forest trees; SE in fruit and forest arid trees; Status of SE in Indian forest trees; SE research in fruit trees in India; Applications of SE for the improvement of tropical fruit trees. Section B comprises 15 chapters, dealing with: SE in oil palm, hazelnut (Corylus avellana L.), pistachio (Pistacia vera L.), Araucaria angustifolia, Quercus suber, Aspidosperma polyneuron, Acacia senegal, Simmondsia chiensis, Cupressus sempervirens, pecan (Carya illinoinensis), rattan (Calamus spp.), tamarillo (Cyphomandra betacea, longan (Dimocarpus longan Lor.), Aegle marmelos, and Euonymus europaeus. Section C comprises three chapters related to cryo-storage of citrus, conifers and rubber. All the chapters have been peer-reviewed and revised accordingly to improve the quality of the chapters. We are thankful to all: (a) contributory authors for their co-operation in submitting manuscripts in time, and (b) reviewers for spending their valuable time in reviewing the manuscripts.

Somatic Embryogenesis in Woody Plants

Author : S.M. Jain,P.K. Gupta,R.J. Newton
Publisher : Springer Science & Business Media
Page : 743 pages
File Size : 47,9 Mb
Release : 2013-11-11
Category : Science
ISBN : 9789401730303

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Somatic Embryogenesis in Woody Plants by S.M. Jain,P.K. Gupta,R.J. Newton Pdf

The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environ mental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Gene rally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taedb), Sandalwood (Santalum album), Citrus, mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propagation for large-scale production of plants or "emblings" or somatic seedlings; b) protoplast work; c) cryopreservation; d) genetic transformation; and e) synthetic or artificial or manufactured seed production.

Somatic Embryogenesis in Woody Plants

Author : S. Mohan Jain,Pramod P.K. Gupta,R.J. Newton
Publisher : Springer Science & Business Media
Page : 512 pages
File Size : 50,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401104913

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Somatic Embryogenesis in Woody Plants by S. Mohan Jain,Pramod P.K. Gupta,R.J. Newton Pdf

The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources and environmental pollution have both accelerated development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which cannot be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying in large number the trees and woody plants. So far, plant tissue culture technology has largely been exploited in the propagation of ornamental plants, especially foliage house plants, by com mercial companies. Generally, tissue culture of woody plants has been recal citrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalurn album), Citrus, Mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propa gation for large-scale production of plants or "emblings" or "somatic embryo plants", b) protoplast work, c) cryopreservation, d) genetic transformation, and e) artificial or manufactured seed production.

Somatic Embryogenesis in Woody Plants

Author : S. Mohan Jain,Pramod P.K. Gupta,R.J. Newton
Publisher : Springer
Page : 416 pages
File Size : 45,9 Mb
Release : 1995-02-28
Category : Gardening
ISBN : STANFORD:36105019376248

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Somatic Embryogenesis in Woody Plants by S. Mohan Jain,Pramod P.K. Gupta,R.J. Newton Pdf

The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put tremendous pressure on trees and tree products; growing awareness of the potential of previously un exploited tree resources and environmental pollution have both accelerated development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which cannot be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying in large number the trees and woody plants. So far, plant tissue culture technology has largely been exploited in the propagation of ornamental plants, especially foliage house plants, by com- mercial companies. Generally, tissue culture of woody plants has been recal- citrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalum album), Citrus, Mango (Mangifera indica), etc. offer a ray of hope of: (a) inexpensive clonal propa- gation for large-scale production of plants or "emblings" or "somatic embryo plants"; (b) protoplast work; (c) cryopreservation; (d) genetic transforma- tion; and (e) artificial or manufactured seed production.

Somatic Embryogenesis

Author : Abdul Mujib,Jozef Šamaj
Publisher : Springer Science & Business Media
Page : 386 pages
File Size : 45,6 Mb
Release : 2006-02-22
Category : Science
ISBN : 3540287175

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Somatic Embryogenesis by Abdul Mujib,Jozef Šamaj Pdf

Somatic embryogenesis, the initiation of embryos from previously differentiated somatic cells, is a unique process in plants. This volume expands our view of a subject that is important for plant biotechnology, genetics, cell biology, development, and agricultural applications. All chapters present the latest research progress, including functional genomic, genetic, and proteomic approaches. A special focus is placed on the effects of stress, environment, and plant growth regulators on embryogenesis. The role of genes such as Leafy Cotyledons and Baby Boom in defining and maintaining cell competence is discussed.

In Vitro Embryogenesis in Higher Plants

Author : Maria Antonietta Germana,Maurizio Lambardi
Publisher : Humana Press
Page : 558 pages
File Size : 54,7 Mb
Release : 2019-03-29
Category : Science
ISBN : 1493979825

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In Vitro Embryogenesis in Higher Plants by Maria Antonietta Germana,Maurizio Lambardi Pdf

This volume presents an overview of recent advances, innovative applications, and future prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five parts: Part I contains reviews on general topics (microspore; zygotic and somatic embryogenesis; in vitro and in vivo asexual embryogenesis; advances on the genetic, physiological, and proteomic knowledge of somatic embryo formation; role of apoptosis and mitochondria in somatic embryogenesis; and innovation in the use of bioreactors). The remaining four parts discuss step-wise protocols on somatic embryogenesis in selected horticultural plants (Part II); forest trees (Part III); gametic embryogenesis (Part IV); and pivotal topics, such as the detection of epigenetics modifications during microspore embryogenesis, the in vitro embryogenesis and plant regenerations from isolated zygotes, the synthetics seed production, the induction and maturation of somatic embryos, and the cryostorage of embryogenic cultures (Part V). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, In Vitro Embryogenesis in Higher Plants, is a useful source of information and ideas for plant tissue culturists, cell biologists, embryologists, horticulturists, and operators of commercial nurseries. This book will introduce the fascinating work in in vitro embryogenesis in higher plants to students and young scientists.

Protocols for Micropropagation of Woody Trees and Fruits

Author : S.Mohan Jain,H. Häggman
Publisher : Springer Science & Business Media
Page : 559 pages
File Size : 47,9 Mb
Release : 2007-09-18
Category : Technology & Engineering
ISBN : 9781402063527

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Protocols for Micropropagation of Woody Trees and Fruits by S.Mohan Jain,H. Häggman Pdf

Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.

Automation and environmental control in plant tissue culture

Author : Jenny Aitken-Christie,T. Kozai,M.A.L Smith
Publisher : Springer Science & Business Media
Page : 576 pages
File Size : 41,8 Mb
Release : 2013-06-29
Category : Science
ISBN : 9789401584616

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Automation and environmental control in plant tissue culture by Jenny Aitken-Christie,T. Kozai,M.A.L Smith Pdf

Automation and Environmental Control in Plant Tissue Culture rigorously explores the new challenges faced by modern plant tissue culture researchers and producers worldwide: issues of cost efficiency, automation, control, and optimization of the in vitro microenvironment. This book achieves a critical balance between the economic, engineering and biological viewpoints, and presents well-balanced, unique, and clearly organized perspectives on current initiatives in the tissue culture arena. Each chapter offers guidelines leading towards an exhaustive, unprecedented level of control over in vitro growth, based on emerging technologies of robotics, machine vision, environmental sensors and regulation, and systems analysis. Unlike other tissue culture books which focus on specific crops and techniques, this book spans the broad range of major tissue culture production systems, and advances evidence on how some underrated aspects of the process actually determine the status of the end product. Key researchers from industry and academia have joined to give up-to-date research evidence and analysis. The collection comprises an essential reference for industrial-scale tissue culture producers, as well as any researcher interested in optimizing in vitro production.