Sub Cellular Proteomics

Author : Harvey Millar,Nicolas L Taylor
Publisher : Frontiers E-books
Page : 255 pages
File Size : 53,5 Mb
Release : 2015-01-05
Category : Botany
ISBN : 9782889193028

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Sub-cellular Proteomics by Harvey Millar,Nicolas L Taylor Pdf

Whilst significant advances have been made in whole organismal proteomics approaches, many researchers still rely on combinations of tissue selection and subcellular prefractionation methods to reduce the complexity of protein extracts from plants prior to proteomic analysis. Often this will allow identification of many lower abundance proteins of the target proteome and it may involve the selection of specific organs, cell types or the isolation of specific subcellular components. These subcellular proteomes provide insight into functions following various treatments and also contribute to the wider understanding of the entire organismal proteome by cataloguing a series of sub-proteome contents. The aim of this Research Topic is to bring together knowledge of sub cellular components in different plant species to provide a basis for accelerated research. It aims to provide a mini-review for each proposed section that summarizes the current understanding of a particular proteome, with the anticipation that every 5 - 10 years we can update these definitive publications.

Author : Eric Bertrand,Michel Faupel
Publisher : Springer Science & Business Media
Page : 397 pages
File Size : 49,5 Mb
Release : 2007-08-29
Category : Science
ISBN : 9781402059438

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Subcellular Proteomics by Eric Bertrand,Michel Faupel Pdf

This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.

Author : Nobuhiro Takahashi,Toshiaki Isobe,Dominic M. Desiderio,Nico M. Nibbering
Publisher : John Wiley & Sons
Page : 275 pages
File Size : 40,7 Mb
Release : 2007-08-31
Category : Science
ISBN : 9780470149645

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Proteomic Biology Using LC/MS by Nobuhiro Takahashi,Toshiaki Isobe,Dominic M. Desiderio,Nico M. Nibbering Pdf

This is one of the first books to focus on the dynamic aspect of proteomes. The book introduces proteomics to the newcomer, reviews the theoretical aspects of proteomics and its state-of-the art technologies, along with a number of biological applications using "classical" proteomic technology. The book also presents a new concept, the Dynamome, or the expression of a comprehensive molecular set that participates in the whole dynamic process of a series of cellular events.

Author : Anonim
Publisher : Academic Press
Page : 377 pages
File Size : 50,6 Mb
Release : 2014-07-03
Category : Business & Economics
ISBN : 9780128005958

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Proteomics in Biomedicine and Pharmacology by Anonim Pdf

Volume 95 of Advances in Protein Chemistry and Structural Biology focuses on advances in proteomic techniques and their application in biomedicine and pharmacology. This volume describes in detail the applications of these techniques in studying a number of diseases and specific protein modifications and profiles, including phosphoproteomics, glycoproteomics, and more. Published continuously since 1944, the Advances in Protein Chemistry and Structural Biology series is the essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins. Each thematically organized volume is guest edited by leading experts in a broad range of protein-related topics. Describes advances in application of powerful techniques in translational research (biomedicine and pharmacology) Chapters are written by authorities in their field Targeted to a wide audience of researchers, specialists, and students The information provided in the volume is well supported by a number of high quality illustrations, figures, and tables

Author : Ganesh K. Agrawal,Randeep Rakwal
Publisher : John Wiley & Sons
Page : 818 pages
File Size : 42,9 Mb
Release : 2008-10-03
Category : Science
ISBN : 9780470369838

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Plant Proteomics by Ganesh K. Agrawal,Randeep Rakwal Pdf

Confidently face the challenges of proteomics research specific to plant science with the information in Plant Proteomics, which will introduce you to the techniques and methodologies required for the study of representative plant species. Read about proteomics studies in Arabidopsis, rice, and legumes and find information about common technologies like mass spectrometry and gel electrophoresis. Discover expression proteomics, functional proteomics, structural proteomics, bioinformatics, and systems biology, understand how to conduct proteomics studies in developing countries and underfunded laboratories, and gain access to guidelines for sample preparation.

Author : Jozef Šamaj,Jay J. Thelen
Publisher : Springer
Page : 369 pages
File Size : 49,9 Mb
Release : 2007-09-11
Category : Science
ISBN : 3540726160

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Plant Proteomics by Jozef Šamaj,Jay J. Thelen Pdf

Plant Proteomics highlights rapid progress in this field, with emphasis on recent work in model plant species, sub-cellular organelles, and specific aspects of the plant life cycle such as signaling, reproduction and stress physiology. Several chapters present a detailed look at diverse integrated approaches, including advanced proteomic techniques combined with functional genomics, bioinformatics, metabolomics and molecular cell biology, making this book a valuable resource for a broad spectrum of readers.

Author : Delphine Pflieger,Jean Rossier
Publisher : Humana Press
Page : 0 pages
File Size : 53,8 Mb
Release : 2010-11-19
Category : Science
ISBN : 1617377724

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Organelle Proteomics by Delphine Pflieger,Jean Rossier Pdf

This is the first book to examine organelle proteomics in depth. It begins by introducing the different analytical strategies developed and successfully utilized to study organelle proteomes, and detailing the use of multidimensional liquid chromatography coupled to tandem mass spectrometry for peptide sample analysis. Detailed protocols are provided and a section is devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle.

Author : Luis Alfonso del Río,Michael Schrader (Professor of cell biology)
Publisher : Unknown
Page : 493 pages
File Size : 55,9 Mb
Release : 2018
Category : SCIENCE
ISBN : 9811322341

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Proteomics of Peroxisomes by Luis Alfonso del Río,Michael Schrader (Professor of cell biology) Pdf

"This new edited volume in the Springer Subcellular Biochemistry Series presents a comprehensive, state-of-the-art overview of the proteomics of peroxisomes derived from mammalian, Drosophila, fungal, and plant origin, and contains contributions from leading experts in the field. The development of sensitive proteomics and mass spectrometry technologies, combined with bioinformatics approaches now allow the identification of low-abundance and transient peroxisomal proteins and permits to identify the complete proteome of peroxisomes, with the consequent increase of our knowledge of the metabolic and regulatory networks of these important cellular organelles. The book lines-up with these developments and is organized in four sections including: (i) mass spectrometry-based organelle proteomics; (ii) prediction of peroxisomal proteomes; (iii) analysis of peroxisome proteome interaction networks; and (iv) peroxisomes in relation to other subcellular compartments"--Publisher's description.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 45,8 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Kay Ohlendieck
Publisher : Springer Nature
Page : 477 pages
File Size : 47,7 Mb
Release : 2022-11-15
Category : Science
ISBN : 9781071628317

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Difference Gel Electrophoresis by Kay Ohlendieck Pdf

The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Author : Jay J. Thelen,Jozef Amaj
Publisher : Unknown
Page : 0 pages
File Size : 46,8 Mb
Release : 2015
Category : Cell interaction
ISBN : OCLC:1389629187

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Plant Proteomics by Jay J. Thelen,Jozef Amaj Pdf

Plant Proteomics highlights rapid progress in this field, with emphasis on recent work in model plant species, sub-cellular organelles, and specific aspects of the plant life cycle such as signaling, reproduction and stress physiology. Several chapters present a detailed look at diverse integrated approaches, including advanced proteomic techniques combined with functional genomics, bioinformatics, metabolomics and molecular cell biology, making this book a valuable resource for a broad spectrum of readers.

Author : Xudong Yao
Publisher : Elsevier
Page : 270 pages
File Size : 40,5 Mb
Release : 2021-10-22
Category : Science
ISBN : 9780128214350

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Advances in Chemical Proteomics by Xudong Yao Pdf

Advances in Chemical Proteomics provides essential concepts and recent applications on probes, tool compounds and concepts for chemical proteomics and then moves on to applications, including solid-phase reagents, fragment screening, designer compounds and protein lipidation. As the second volume in the Developments in Organic Chemistry series, each chapter is written by experts in the field. Users will find this to be a valuable reference for organic chemists and chemical biologists who are interested in developing tool compounds and reagents to measure and interrogate proteome, develop drug leads, and measure off-target effects and drug toxicity. Analytical chemists who are interested in better understanding organic chemistry behind commonly used reagents for quantitative proteomics and tools compounds in the emerging field of chemical proteomics will also benefit from this comprehensive resource on the topics presented. Provides an ideal, introductory book to chemical proteomics for organic chemists, pharmaceutical chemists and chemical biologists Includes advanced, recent applications and reviews in chemical proteomics Presents valuable work by a global team of experts from the field of proteomics

Author : Andrew J. Link
Publisher : Springer Science & Business Media
Page : 585 pages
File Size : 50,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595846

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2-D Proteome Analysis Protocols by Andrew J. Link Pdf

With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

Author : Luis A. Del Rio,Michael Schrader
Publisher : Subcellular Biochemistry
Page : 508 pages
File Size : 51,9 Mb
Release : 2019-11-23
Category : Medical
ISBN : 9811347530

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Proteomics of Peroxisomes by Luis A. Del Rio,Michael Schrader Pdf

This new edited volume in the Springer Subcellular Biochemistry Series presents a comprehensive, state-of-the-art overview of the proteomics of peroxisomes derived from mammalian, Drosophila, fungal, and plant origin, and contains contributions from leading experts in the field. The development of sensitive proteomics and mass spectrometry technologies, combined with bioinformatics approaches now allow the identification of low-abundance and transient peroxisomal proteins and permits to identify the complete proteome of peroxisomes, with the consequent increase of our knowledge of the metabolic and regulatory networks of these important cellular organelles. The book lines-up with these developments and is organized in four sections including: (i) mass spectrometry-based organelle proteomics; (ii) prediction of peroxisomal proteomes; (iii) analysis of peroxisome proteome interaction networks; and (iv) peroxisomes in relation to other subcellular compartments. The editor Luis A. del Río is Professor ad honorem of the Spanish National Research Council (CSIC) in the Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry and Cell & Molecular Biology of Plants, at the Estación Experimental del Zaidín, Granada, Spain. Del Río's research group focuses on the metabolism of reactive oxygen species (ROS), reactive nitrogen species (RNS) and antioxidants in plant peroxisomes, and the ROS- and RNS-dependent role of peroxisomes in plant cell signalling. The editor Michael Schrader is Professor of Cell Biology & Cytopathology in the Department of Biosciences at the University of Exeter, UK. Using mammalian peroxisomes as model organelles, Prof. Schrader and his team aim to unravel the molecular machinery and signalling pathways that mediate and regulate the formation, dynamics and abundance of these medically relevant cellular compartments.

Author : Djuro Josic,Douglas C. Hixson
Publisher : Humana Press
Page : 346 pages
File Size : 49,5 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493962140

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Liver Proteomics by Djuro Josic,Douglas C. Hixson Pdf

The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In Liver Proteomics: Methods and Protocols, expert researchers in the field detail many of the methods that are used to study the live. These methods include the most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Liver Proteomics: Methods and Protocols seeks to aid scientists in the further study of this crucially important organ.