Superantigen Protocols

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Superantigen Protocols

Teresa Krakauer
Superantigen Protocols Book PDF

Author : Teresa Krakauer
Publisher : Springer Science & Business Media
Page : 259 pages
File Size : 49,9 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781592593675

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Superantigen Protocols by Teresa Krakauer Pdf

Leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of superantigens. Their protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level..

Superantigens

Amanda J. Brosnahan
Superantigens Book PDF

Author : Amanda J. Brosnahan
Publisher : Humana Press
Page : 228 pages
File Size : 42,9 Mb
Release : 2019-03-29
Category : Medical
ISBN : 1493980262

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Superantigens by Amanda J. Brosnahan Pdf

This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Superantigens: Methods and Protocols contains a detailed and wide breadth of subject coverage that any scientist, clinician, or industry professional interested in this field will find valuable.

Viral Superantigens

Kyuhei Tomonari
Viral Superantigens Book PDF

Author : Kyuhei Tomonari
Publisher : CRC Press
Page : 284 pages
File Size : 46,9 Mb
Release : 1997-05-30
Category : Medical
ISBN : 0849376882

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Viral Superantigens by Kyuhei Tomonari Pdf

Since the discovery of viral superantigens in 1991, immunologists have made a number of new discoveries. The discoveries, especially those relating to the interplay between the immune system and viruses producing superantigens, have had a great impact on immunology and virology, as it appears that some diseases are triggered or exacerbated by viral superantigens. Viral Superantigens presents a complete review of this new area of study. Edited by a leading researcher and authored by a distinguished team of contributors, this comprehensive analysis covers every aspect of viral superantigens and related subjects, including critical topics such as effects on the T cell repertoire and viral superantigen-mediated diseases. Immunologists and virologists, clinical practitioners, and graduate students will find this book an invaluable resource to encourage further advances in research.

Superantigens

Brigitte T. Huber,Ed Palmer
Superantigens Book PDF

Author : Brigitte T. Huber,Ed Palmer
Publisher : CSHL Press
Page : 198 pages
File Size : 40,8 Mb
Release : 1993
Category : Medical
ISBN : 0879693983

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Superantigens by Brigitte T. Huber,Ed Palmer Pdf

Superantigens are microbial products that defy the conventional notion of lymphocyte activation to produce an unusually vigorous, genetically unrestricted response among many subsets of antigen-specific T cells. Bacterial toxins and certain viral gene products behave as superantigens, deleting T cells in vivo and stimulating their proliferation in vitro. These observations raise many fascinating questions about the nature of T-cell activation and microbial pathogenesis. This volume is an up-to-date, wide-ranging examination of the function and significance of superantigens in virology, biochemistry, and cell and stuctural biology as well as immunology. The book will interest graduate students and postdoctoral fellows in a wide range of biomedical sciences.

MHC Protocols

Stephen H. Powis,Robert W. Vaughan
MHC Protocols Book PDF

Author : Stephen H. Powis,Robert W. Vaughan
Publisher : Springer Science & Business Media
Page : 343 pages
File Size : 44,5 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781592592913

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MHC Protocols by Stephen H. Powis,Robert W. Vaughan Pdf

The aim of MHC Protocols is to document protocols that can be used for the analysis of genetic variation within the human major histocompatibility complex (MHC; HLA region). The human MHC encompasses approximately 4 million base pairs on the short arm of chromosome 6 at cytogenetic location 6p21. 3. The region is divided into three subregions. The telomeric class I region contains the genes that encode the HLA class I molecules HLA-A, -B, and -C. The centromeric class II region contains the genes encoding the HLA class II molecules HLA-DR, -DQ, and -DP. In between is the class III region, originally identified because it contains genes encoding components of the complement pathway. The entire human MHC has recently been sequenced (1) and each subregion is now known to contain many other genes, a number of which have immunological functions. The study of polymorphism within the MHC is well established, because the region contains the highly polymorphic HLA genes. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. As a result, large numbers of HLA alleles have been identified, a process that has been further driven by recent interest in HLA gene diversity in ethnic populations. The extreme genetic variation in HLA genes is believed to have been driven by the evolutionary response to infectious agents, but relatively few studies have analyzed associations between HLA genetic variation and infectious disease, which has been difficult to demonstrate.

Inflammation Protocols

Paul G. Winyard,Derek A. Willoughby
Inflammation Protocols Book PDF

Author : Paul G. Winyard,Derek A. Willoughby
Publisher : Springer Science & Business Media
Page : 373 pages
File Size : 44,7 Mb
Release : 2008-02-03
Category : Medical
ISBN : 9781592593743

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Inflammation Protocols by Paul G. Winyard,Derek A. Willoughby Pdf

Inflammation has been described as the basis of many pathologies of human disease. When one considers the updated signs of inflammation, they would be vasodilation, cell migration, and, in the case of chronic inflam- tion, cell proliferation, often with an underlying autoimmune basis. Gen- ally, inflammation may be divided into acute, chronic, and autoimmune, - though the editors believe that most, if not all, chronic states are often the result of an autoimmune response to an endogenous antigen. Thus, a proper understanding of the inflammatory basis may provide clues to new therap- tic targets not only in classical inflammatory diseases, but atherosclerosis, cancer, and ischemic heart disease as well. The lack of advances in classical inflammatory diseases, such as rh- matoid arthritis, may in part arise from a failure to classify the disease into different forms. That different forms exist is exemplified in patients with d- fering responses to existing antiinflammatory drugs, ranging from nonresponders to very positive responders for a particular nonsteroidal an- inflammatory drug (NSAID). Though researchers have progressively unr- eled the mechanisms, the story is far from complete. It should also be noted that the inflammatory response is part of the innate immune response, or to use John Hunter’s words in 1795, “inflammation is a salutary response.” That may be applied in particular to the defensive response to invading micro- ganisms.

RT-PCR Protocols

Nicola King,Joe O’Connell
RT PCR Protocols Book PDF

Author : Nicola King,Joe O’Connell
Publisher : Springer Science & Business Media
Page : 370 pages
File Size : 46,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592592838

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RT-PCR Protocols by Nicola King,Joe O’Connell Pdf

Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.

Peptide Research Protocols

Janet J. Maguire,Anthony P. Davenport
Peptide Research Protocols Book PDF

Author : Janet J. Maguire,Anthony P. Davenport
Publisher : Springer Science & Business Media
Page : 241 pages
File Size : 52,6 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592592890

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Peptide Research Protocols by Janet J. Maguire,Anthony P. Davenport Pdf

A panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of the endothelin system from its synthesis and metabolism, to its function in health and disease. Theses methods use state-of-the-art molecular techniques to quantify the expression of mRNA for both endothelin receptors and the endothelin converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.

PCR Mutation Detection Protocols

Bimal D. M. Theophilus,Ralph Rapley
PCR Mutation Detection Protocols Book PDF

Author : Bimal D. M. Theophilus,Ralph Rapley
Publisher : Springer Science & Business Media
Page : 214 pages
File Size : 53,5 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592739

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PCR Mutation Detection Protocols by Bimal D. M. Theophilus,Ralph Rapley Pdf

1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

Protein Sequencing Protocols

Bryan John Smith
Protein Sequencing Protocols Book PDF

Author : Bryan John Smith
Publisher : Springer Science & Business Media
Page : 489 pages
File Size : 45,5 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592593422

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Protein Sequencing Protocols by Bryan John Smith Pdf

Determination of the protein sequence is as important today as it was a half century ago, even though the techniques and purposes have changed over time. Mass spectrometry has continued its recent rapid development to find notable application in the characterization of small amounts of protein, for example, in the field of proteomics. The “traditional” chemical N-terminal sequencing is still of great value in quality assurance of the increasing number of biopharmaceuticals that are to be found in the clinic, checking processing events of recombinant proteins, and so on. It is joined in the armory of me- ods of protein analysis by such techniques as C-terminal sequencing and amino acid analysis. These methods are continually developing. The first edition of Protein Sequencing Protocols was a “snapshot” of methods in use in protein biochemistry laboratories at the time, and this, the second edition, is likewise. Methods have evolved in the intervening period, and the content of this book has similarly changed, the content of some chapters having been superceded and replaced by other approaches. Thus, in this edition, there is inclusion of approaches to validation of methods for quality assurance work, reflecting the current importance of biopharmaceuticals, and also a guide to further analysis of protein sequence information, acknowledging the importance of bioinformatics.

Liposome Methods and Protocols

Subhash C. Basu,Manju Basu
Liposome Methods and Protocols Book PDF

Author : Subhash C. Basu,Manju Basu
Publisher : Springer Science & Business Media
Page : 250 pages
File Size : 47,8 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592591756

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Liposome Methods and Protocols by Subhash C. Basu,Manju Basu Pdf

In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Protocols is to bring together a wide range of detailed laboratory protocols covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each protocol chapter we have detailed the materials to be used, followed by a step-by-step protocol. The Notes section of each protocol is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.

Protein Kinase C Protocols

Alexandra C. Newton
Protein Kinase C Protocols Book PDF

Author : Alexandra C. Newton
Publisher : Springer Science & Business Media
Page : 565 pages
File Size : 53,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592593972

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Protein Kinase C Protocols by Alexandra C. Newton Pdf

Since the discovery that protein kinase C (PKC) transduces the ab- dance of signals that result in phospholipid hydrolysis, this enzyme has been at the forefront of research in signal transduction. Protein Kinase C Protocols covers fundamental methods for studying the structure, function, regulation, subcellular localization, and macromolecular interactions of PKC. Protein Kinase C Protocols is divided into 11 sections representing the major aspects of PKC regulation and function. Part I contains an introduction and a historical perspective on the discovery of PKC by Drs. Yasutomi Nishizuka and Ushio Kikkawa. Part II describes methods to purify PKC. Part III describes the standard methods for measuring PKC activity: its enzymatic activity and its stimulus-dependent translocation from the cytosol to the membrane. Part IV describes methods for measuring the membrane interaction of PKC in vivo and in vitro. Part V provides methodologies and techniques for measuring the ph- phorylation state of PKC, including a protocol for measuring the activity of PKC’s upstream kinase, PDK-1. Novel methods for identifying substrates are described in Part VI. Part VII presents protocols for expressing and analyzing the membrane targeting domains of PKC. Part VIII provides a comprehensive c- pilation of methods used to identify binding partners for PKC. Part IX describes pharmacological probes used to study PKC. The book ends with a presentation of genetic approaches to study PKC (Part X) and a discussion of approaches used to study PKC in disease (Part XI).

E. coli Gene Expression Protocols

Peter E. Vaillancourt
E coli Gene Expression Protocols Book PDF

Author : Peter E. Vaillancourt
Publisher : Springer Science & Business Media
Page : 347 pages
File Size : 47,9 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592593019

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E. coli Gene Expression Protocols by Peter E. Vaillancourt Pdf

Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.

Lentivirus Gene Engineering Protocols

Maurizio Federico
Lentivirus Gene Engineering Protocols Book PDF

Author : Maurizio Federico
Publisher : Springer Science & Business Media
Page : 310 pages
File Size : 52,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592593934

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Lentivirus Gene Engineering Protocols by Maurizio Federico Pdf

Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger. Lentivirus vector-based technologies represent the last frontier in the development of powerful and reliable methods for both in vitro and in vivo gene transfer in eukaryotic animal cells. Although the design of lentivirus vectors is closely reminiscent of those already successfully applied to the construction of oncoretroviral vectors, some unique features, e.g., the ef- ciency in transducing both postmitotic and stem cells, render the use of lentivirus vectors invaluable. It has been a great pleasure to edit Lentivirus Gene Engineering Pro- cols, owing in part to the high level of enthusiasm that the authors dem- strated in contributing to this book. The fact that so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical and practical aspects of the origin and the development of diverse lentivirus vector types. This, in my opinion, rep- sents a unique added value of this volume, which should help our work resist the passage of time, to which books such as this are particularly sensitive.

Transgenic Mouse Methods and Protocols

Marten H. Hofker,Jan van Deursen
Transgenic Mouse Methods and Protocols Book PDF

Author : Marten H. Hofker,Jan van Deursen
Publisher : Springer Science & Business Media
Page : 382 pages
File Size : 44,8 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592593408

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Transgenic Mouse Methods and Protocols by Marten H. Hofker,Jan van Deursen Pdf

Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.