The Nucleic Acid Protocols Handbook

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The Nucleic Acid Protocols Handbook

Ralph Rapley
The Nucleic Acid Protocols Handbook Book PDF

Author : Ralph Rapley
Publisher : Springer Science & Business Media
Page : 478 pages
File Size : 51,5 Mb
Release : 2008-06-29
Category : Science
ISBN : 9781592590384

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The Nucleic Acid Protocols Handbook by Ralph Rapley Pdf

A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."

Handbook of Nucleic Acid Purification

Dongyou Liu
Handbook of Nucleic Acid Purification Book PDF

Author : Dongyou Liu
Publisher : CRC Press
Page : 584 pages
File Size : 40,9 Mb
Release : 2009-01-14
Category : Science
ISBN : 9781420070972

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Handbook of Nucleic Acid Purification by Dongyou Liu Pdf

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences. The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing volume on nucleic acid extraction strategies. Due to the complexities within prokaryotic and eukaryotic cells, purification of the nucleic acids often forms a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens. Bringing together contributions from leading researchers, the handbook presents a comprehensive catalog of nucleic acid isolation methods. It includes dedicated sections on strategies for viruses, bacteria, fungi, parasites, insects, mammals, and plants, as well as for ancient samples, with an additional emphasis on sample preparation methods for direct molecular applications. Each chapter in this handbook: Explores the biological background important to understanding specific organisms and specimens Reviews principles and current techniques for efficient isolation Discusses challenges and future trends relating to improved recovery of nucleic acids Besides providing an updated, reliable reference for anyone with an interest in molecular biology, this book offers a practical guide for clinical, forensic, and research scientists involved in molecular analysis of biological specimens. It also constitutes a convenient resource for students in other areas of biological sciences, and an indispensable roadmap for both new and experienced researchers wishing to acquire or sharpen their skills in nucleic acid preparation.

Basic DNA and RNA Protocols

Adrian J. Harwood
Basic DNA and RNA Protocols Book PDF

Author : Adrian J. Harwood
Publisher : Unknown
Page : 534 pages
File Size : 42,5 Mb
Release : 1996-02-19
Category : Medical
ISBN : UOM:39015036527144

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Basic DNA and RNA Protocols by Adrian J. Harwood Pdf

In Basic DNA and RNA Protocols experienced researchers and innovators present an essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. Its outstanding, thoroughly up-to-date, and eminently practical protocols make it an essential tool for today's molecular biologist.

Molecular Biomethods Handbook

John M. Walker,Ralph Rapley
Molecular Biomethods Handbook Book PDF

Author : John M. Walker,Ralph Rapley
Publisher : Springer Science & Business Media
Page : 1148 pages
File Size : 48,8 Mb
Release : 2008-09-04
Category : Science
ISBN : 1603273743

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Molecular Biomethods Handbook by John M. Walker,Ralph Rapley Pdf

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.

Peptide Nucleic Acids

Peter E. Nielsen
Peptide Nucleic Acids Book PDF

Author : Peter E. Nielsen
Publisher : Springer Science & Business Media
Page : 270 pages
File Size : 53,8 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592592906

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Peptide Nucleic Acids by Peter E. Nielsen Pdf

Peptide nucleic acids (PNAs) have now existed for slightly more than ten years, with the interest in and applications of this pseudopeptide DNA mimic steadily increasing during the entire period. PNAs have rapidly attracted the attention of scientists from a diversity of fields ranging from (bio)organic and biophysical chemistry to prebiotic evolution, and from molecular biology to genetic diagnostics and drug development. Many of the applications take advantage of the unique properties of PNA—an uncharged pseudopeptide—that distinguish this DNA mimic from more traditional DNA analogs. Rather than trying to create a comprehensive collection of all published methods and protocols involving PNA—many of which have not yet been validated— I have decided to concentrate on select protocols that are either very well established by several groups around the world, such as PCR-clamping and in situ hybridization, or on new methods that may have broader future impact. Basic methods for PNA oligomer synthesis and analyses have also been included. I am very grateful to those friends and colleagues who have enthusiastically contributed their work, discussions, and writing, and thereby made this book possible. Peter E. Nielsen v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesis of PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . .

Nucleic Acid Detection

Dmitry M. Kolpashchikov,Yulia V. Gerasimova
Nucleic Acid Detection Book PDF

Author : Dmitry M. Kolpashchikov,Yulia V. Gerasimova
Publisher : Humana Press
Page : 0 pages
File Size : 47,6 Mb
Release : 2013-09-13
Category : Medical
ISBN : 1627035346

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Nucleic Acid Detection by Dmitry M. Kolpashchikov,Yulia V. Gerasimova Pdf

In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.

Essentials of Nucleic Acid Analysis

Jacquie T. Keer,Lyndsey Birch
Essentials of Nucleic Acid Analysis Book PDF

Author : Jacquie T. Keer,Lyndsey Birch
Publisher : Royal Society of Chemistry
Page : 274 pages
File Size : 42,7 Mb
Release : 2008
Category : Science
ISBN : 9780854043675

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Essentials of Nucleic Acid Analysis by Jacquie T. Keer,Lyndsey Birch Pdf

An indispensable handbook of the highest standard for those working in the fields of food analysis and forensic applications.

Peptide Nucleic Acids

Peter E Nielsen,Daniel H. Appella
Peptide Nucleic Acids Book PDF

Author : Peter E Nielsen,Daniel H. Appella
Publisher : Humana
Page : 0 pages
File Size : 49,6 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493959522

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Peptide Nucleic Acids by Peter E Nielsen,Daniel H. Appella Pdf

Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Peptide Nucleic Acids: Methods and Protocols, Second Edition serves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.

PCR Protocols

John M. S. Bartlett,David Stirling
PCR Protocols Book PDF

Author : John M. S. Bartlett,David Stirling
Publisher : Springer Science & Business Media
Page : 1083 pages
File Size : 51,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592593842

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PCR Protocols by John M. S. Bartlett,David Stirling Pdf

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Peptide Nucleic Acids

Peter E. Nielsen
Peptide Nucleic Acids Book PDF

Author : Peter E. Nielsen
Publisher : Garland Science
Page : 332 pages
File Size : 50,9 Mb
Release : 2004
Category : DNA
ISBN : 0954523245

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Peptide Nucleic Acids by Peter E. Nielsen Pdf

Peptide Nucleic Acids, Second Edition has been extensively revised, updated, and enlarged to contain many new chapters covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of peptide nucleic acids. Concepts are clearly explained with each chapter containing concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area.

Gel Electrophoresis: Nucleic Acids

Dr Robin Martin
Gel Electrophoresis Nucleic Acids Book PDF

Author : Dr Robin Martin
Publisher : Garland Science
Page : 190 pages
File Size : 52,9 Mb
Release : 2020-08-26
Category : Science
ISBN : 9781000101430

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Gel Electrophoresis: Nucleic Acids by Dr Robin Martin Pdf

Through its clear presentation of the basic concepts, Gel Electrophoresis: Nucleic Acids breaks new ground by describing the principles of the technique without resorting to complicated protocols and recipes.

RNA Isolation and Characterization Protocols

Ralph Rapley,David L. Manning
RNA Isolation and Characterization Protocols Book PDF

Author : Ralph Rapley,David L. Manning
Publisher : Humana Press
Page : 264 pages
File Size : 43,7 Mb
Release : 2013-08-18
Category : Science
ISBN : 1489942505

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RNA Isolation and Characterization Protocols by Ralph Rapley,David L. Manning Pdf

Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.

G-Quadruplex Nucleic Acids

Danzhou Yang,Clement Lin
G Quadruplex Nucleic Acids Book PDF

Author : Danzhou Yang,Clement Lin
Publisher : Humana
Page : 437 pages
File Size : 44,6 Mb
Release : 2020-09-07
Category : Medical
ISBN : 1493996681

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G-Quadruplex Nucleic Acids by Danzhou Yang,Clement Lin Pdf

This volume covers the structures, properties, and functions of G-quadruplexes in a wide range of biological disciplines, including therapeutic intervention and biomaterial application. The chapters in this book explore a wide range of vital and new experimental techniques used in the study of G-quadruplexes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, G-Quadruplex DNA: Methods and Protocols is a valuable resource for both novice and experienced researchers who work in biophysics, structural biology, computational biology, biochemistry, and molecular and cell biology, and who want to learn more about the potential roles and effects of G-quadruplex in these fields.

Nucleic Acid Detection and Structural Investigations

Kira Astakhova,Syeda Atia Bukhari
Nucleic Acid Detection and Structural Investigations Book PDF

Author : Kira Astakhova,Syeda Atia Bukhari
Publisher : Humana
Page : 270 pages
File Size : 41,7 Mb
Release : 2020-11-14
Category : Science
ISBN : 1071601407

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Nucleic Acid Detection and Structural Investigations by Kira Astakhova,Syeda Atia Bukhari Pdf

This book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing in vitro detection, nanotechnology and imaging, biomedical applications, and big data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nucleic Acid Detection and Structural Investigations: Methods and Protocols aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences.

PCR Protocols

Bruce A. White
PCR Protocols Book PDF

Author : Bruce A. White
Publisher : Springer Science & Business Media
Page : 397 pages
File Size : 55,8 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595020

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PCR Protocols by Bruce A. White Pdf

PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.