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Author : Jacquie T. Keer,Lyndsey Birch
Publisher : Royal Society of Chemistry
Page : 274 pages
File Size : 43,6 Mb
Release : 2008
Category : Science
ISBN : 9780854043675
An indispensable handbook of the highest standard for those working in the fields of food analysis and forensic applications.
Author : Elena Hilario,John F. MacKay
Publisher : Springer Science & Business Media
Page : 318 pages
File Size : 44,8 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781597452298
Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author : Dmitry M. Kolpashchikov,Yulia V. Gerasimova
Publisher : Humana Press
Page : 0 pages
File Size : 52,6 Mb
Release : 2013-09-13
Category : Medical
ISBN : 1627035346
In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.
Author : Ralph Rapley
Publisher : Springer Science & Business Media
Page : 478 pages
File Size : 42,5 Mb
Release : 2008-06-29
Category : Science
ISBN : 9781592590384
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Author : Charles A. Dangler
Publisher : Wiley-Liss
Page : 304 pages
File Size : 52,6 Mb
Release : 1996-06-14
Category : Medical
ISBN : UOM:39015038528702
Nucleic acid hybridizations: principles and strategies; Generation of nucleic acid probe molecules; Nonisotopic DNA labeling strategies; Approaches to genetic analysis: genetic disease and screening; Approaches to genetic analysis: forensics; Approaches to infectious disease diagnosis; Diagnostic nucleic acid probe technology in veterinary medicine; Diagnostic nucleic acid probes in human medicine: applications, present and future; Developments in food technology: applications and regulatory and economic considerations; Current development and applications of nucleic acid technology in the environmental sciences.
Author : Chang-Hui Shen
Publisher : Elsevier
Page : 590 pages
File Size : 49,7 Mb
Release : 2023-06-29
Category : Science
ISBN : 9780323986090
Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. Provides explanations on how techniques are used to diagnosis at the molecular level Explains how to use information technology to communicate and assess results in the lab Enhances our understanding of fundamental molecular biology and places techniques in context Places protocols into context with practical applications Includes extra chapters on respiratory viruses (Corona)
Author : Gregory W. Warr,James D. Karam,Lee Chao
Publisher : CRC Press
Page : 452 pages
File Size : 40,9 Mb
Release : 1990-11-26
Category : Medical
ISBN : 0849353114
Methods in Nucleic Acids Research provides extensively referenced overviews of chapter topics, in addition to step-by-step laboratory protocols. Topics include discussions regarding the preparation and assay of antibodies against oligopeptides, RNA footprinting, gel-retardation assays for nucleic acid binding proteins, in vitro transcription and translation assays for studies of eukaryotic gene expression, human genome mapping, forensic analysis of DNA polymorphism, in situ hybridization for the detection of specific RNA, and other methods. Biochemists, molecular biologists, immunologists, cell biologists, and geneticists will find this book invaluable for their research.
Author : Yi-Wei Tang,Charles W. Stratton
Publisher : Springer Science & Business Media
Page : 550 pages
File Size : 51,7 Mb
Release : 2007-01-16
Category : Medical
ISBN : 9780387328928
Clinical microbiologists are engaged in the field of diagnostic microbiology to determine whether pathogenic microorganisms are present in clinical specimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. During the past two decades, technical advances in the field of diagnostic microbiology have made constant and enormous progress in various areas, including bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecular aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendly software have significantly broadened the diagnostic arsenal for the clinical microbiologist. The conventional diagnostic model for clinical microbiology has been labor-intensive and frequently required days to weeks before test results were available. Moreover, due to the complexity and length of such testing, this service was usually directed at the hospitalized patient population. The physical structure of laboratories, staffing patterns, workflow, and turnaround time all have been influenced profoundly by these technical advances. Such changes will undoubtedly continue and lead the field of diagnostic microbiology inevitably to a truly modern discipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleic acid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several "hot" spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field. We hope our readers like this technique-based approach and your feedback is highly appreciated. We want to thank the authors who devoted their time and efforts to produce their chapters. We also thank the staff at Springer Press, especially Melissa Ramondetta, who initiated the whole project. Finally, we greatly appreciate the constant encouragement of our family members through this long effort. Without their unwavering faith and full support, we would never have had the courage to commence this project.
Author : Lela Buckingham
Publisher : F.A. Davis
Page : 576 pages
File Size : 54,5 Mb
Release : 2019-02-22
Category : Medical
ISBN : 9780803699540
Meet the challenges of this rapidly expanding field with a solid understanding of the fundamentals of nucleic acid biochemistry as well as the advanced concepts integral to practice in today’s laboratories. With a focus on the application of molecular concepts to the diagnosis of disease, the 3rd Edition of this popular resource encompasses microbiology, virology, genetics, oncology, and human identification.
Author : Andreas Keller,Eckart Meese
Publisher : John Wiley & Sons
Page : 390 pages
File Size : 45,8 Mb
Release : 2015-01-12
Category : Science
ISBN : 9783527335565
By integrating technology, supporting infrastructure and efficient application, the all-in-one guide presents molecular diagnostics as an essential component of modern, personalized clinical practice. It considers all important aspects, from the hardware and software needed, to recent improvements in blood- and non-blood-based biomarker tests. Chapters on ethical challenges and a look at current trends and the latest innovations are also included. Bridging the gap between industry and academia, this is a highly useful resource for practitioners as well as for developers of modern, DNA- and RNA-based molecular diagnostics.
Author : Terence Austen Brown
Publisher : Oxford University Press, USA
Page : 640 pages
File Size : 41,6 Mb
Release : 1991
Category : Molecular cloning
ISBN : 0199631158
The two volumes of Essential Molecular Biology: A Practical Approach provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers. Volume I concentrates on the preparation, purification and handling of nucleic acids, and includes chapters on microbiological procedures and the construction and use of recombinant DNA molecules. Volume II covers the procedures needed for identification of specific genes and the study of their structure and cellular expression. Topics covered include preparation of gene libraries, hybridization methods, nucleic acid sequencing, DNA mapping, and DNA amplification by the polymerase chain reaction. Each chapter is written in an accessible style by an acknowledged expert in that field with the emphasis on providing sound technical instruction, in combination with the necessary theoretical background and useful troubleshooting guidance. This book brings powerful techniques to the forefront of molecular biology research. Together, the two volumes provide a comprehensive molecular biology manual tailored specially for the novice.
Author : Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders
Publisher : Taylor & Francis
Page : 362 pages
File Size : 44,6 Mb
Release : 2004
Category : Polymerase chain reaction
ISBN : 9781134184002
Author : Adrian J. Harwood
Publisher : Unknown
Page : 534 pages
File Size : 55,8 Mb
Release : 1996-02-19
Category : Medical
ISBN : UOM:39015036527144
In Basic DNA and RNA Protocols experienced researchers and innovators present an essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. Its outstanding, thoroughly up-to-date, and eminently practical protocols make it an essential tool for today's molecular biologist.
Author : Marcelo Larramendy,Sonia Soloneski
Publisher : BoD – Books on Demand
Page : 222 pages
File Size : 48,8 Mb
Release : 2016-03-16
Category : Science
ISBN : 9789535122647
This edited book, "Nucleic Acids - From Basic Aspects to Laboratory Tools", contains a series of chapters that highlight the development and status of the various aspects of the nucleic acids related to DNA chemistry and biology and the molecular application of these small DNA molecules and related synthetic analogues within biological systems. Furthermore, it is hoped that the information in the present book will be of value to those directly engaged in the handling and use of nucleic acids, and that this book will continue to meet the expectations and needs of all who are interested in the different fascinating aspects of molecular biology.
Author : Francois Ferre
Publisher : Springer Science & Business Media
Page : 379 pages
File Size : 54,8 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461241645
Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.