Tracers In Metabolic Research

Author : Robert R. Wolfe,David L. Chinkes
Publisher : John Wiley & Sons
Page : 494 pages
File Size : 43,9 Mb
Release : 2004-10-18
Category : Medical
ISBN : 0471462098

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Isotope Tracers in Metabolic Research by Robert R. Wolfe,David L. Chinkes Pdf

In the past few years, the number of applications of tracers for in vivo biomedical studies has greatly increased. New analytical tools at the genetic and protein levels have spurred this growth, opening the door for a deeper understanding of metabolic events. This in turn promises to yield significant advances in the understanding and treatment of human disease. Now fully revised and expanded, Isotope Tracers in Metabolic Research, Second Edition is the established definitive text on stable and radioactive isotope tracers. In unique, multidisciplinary fashion, it presents comprehensive coverage of new methodological, mathematical, and theoretical approaches. This new Second Edition includes: All-new chapters on nuclear magnetic resonance, mass isotopomer analysis, and methods of protein metabolism analysis A completely updated categorized list of over 750 references Major advances in the development of mass isotopomer and positional isotopomer techniques, noninvasive isotope techniques for studying metabolic pathways, hyphenated techniques, and new tracer techniques The latest developments in quantification of DNA synthesis and mass spectrometry spurred by genome sequencing and proteomics New coverage of mathematical modeling Expanded coverage of microdialysis probes, laboratory procedures, and regulatory issues related to human studies In this complete guide to performing tracer studies, the authors systematically cover tracer selection, modeling considerations, sample derivitization, mass spectrometry analysis, and data interpretation. Problems and discussion questions highlight key points in each chapter. Isotope Tracers in Metabolic Research, Second Edition offers students and researchers a comprehensive, practical resource for utilizing the latest tracer methodologies.

Author : Robert R. Wolfe
Publisher : Unknown
Page : 312 pages
File Size : 54,5 Mb
Release : 1984
Category : Medical
ISBN : UOM:39015006719325

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Tracers in Metabolic Research by Robert R. Wolfe Pdf

Methods for studying metabolism. Determination of substrate kinetics. Constant isotope infusion technique. Determination of substrate kinetics. Bolus-injection technique. Determination of substrate oxidation. Bicarbonate kinetics and natural enrichment. Priming the pool. Nonsteady state kinetics. Selection of sites for infusing isotope andj drawing samples. Specific application. Glucose metabolism. Specific application. Urea. Specific application. Fat meltabolism. Amino acids. Whole-body protein turnover. Total body water. Clinical application of stable isotopes. Analytical methods. Instrumentation. Mass spectrometry. Selected ion monitoring. Preparation of samples. Chromatography. Determination of specific radioactivity. Collection of CO2. Isotope ratio mass spectrometry techniques. Gas chromatography-mass spectrometry methods. In vivo approaches. Isotopic tracers. Advantages of stable isotopes. Biological effects. Enzymatic effects. Definition of terms. Influence of uptake on calculation of rate of appearance. Influence of pool size and complexity. Net movement of tracer and tracee. Clearance. Stable versus radioisotopes. Single-pool analysis. Bicarbonate recovery. Natural abundance of 13C. Practical consideration of performing recovery studies. Determining NaH13 CO3 infusion rate. Determining VCO2. Theory of priming dose. Priming secondary pools. Steele equation. In vivo assessment of steele equation. Glucose. Effective volume of distribution. Urea. Arterial versus central venous infusion.

Author : Robert R. Wolfe
Publisher : Unknown
Page : 300 pages
File Size : 42,9 Mb
Release : 1984-06
Category : Electronic
ISBN : 0471834386

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Tracers in Metabolic Research by Robert R. Wolfe Pdf

Author : Institute of Medicine,Committee on Military Nutrition Research
Publisher : National Academies Press
Page : 729 pages
File Size : 46,9 Mb
Release : 1997-09-03
Category : Medical
ISBN : 9780309174534

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Emerging Technologies for Nutrition Research by Institute of Medicine,Committee on Military Nutrition Research Pdf

The latest of a series of publications based on workshops sponsored by the Committee on Military Nutrition Research, this book's focus on emerging technologies for nutrition research arose from a concern among scientists at the U.S. Army Research Institute of Environmental Medicine that traditional nutrition research, using standard techniques, centered more on complex issues of the maintenance or enhancement of performance, and might not be sufficiently substantive either to measure changes in performance or to predict the effects on performance of stresses soldiers commonly experience in operational environments. The committee's task was to identify and evaluate new technologies to determine whether they could help resolve important issues in military nutrition research. The book contains the committee's summary and recommendations as well as individually authored chapters based on presentations at a 1995 workshop. Other chapters cover techniques of body composition assessment, tracer techniques for the study of metabolism, ambulatory techniques for the determination of energy expenditure, molecular and cellular approaches to nutrition, the assessment of immune function, and functional and behavioral measures of nutritional status.

Author : E. Gurpide
Publisher : Springer Science & Business Media
Page : 200 pages
File Size : 47,8 Mb
Release : 2013-03-08
Category : Medical
ISBN : 9783642808920

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Tracer Methods in Hormone Research by E. Gurpide Pdf

The purpose of this monograph is to describe theoretical aspects of the interpretation of data obtained from experiments performed with labeled hormones. Quantitative endocrinologic studies involving the use of tracers include the determination of rates at which hormones are secreted by endocrine glands and are produced outside these glands by conversion of other secreted hor mones. Tracer experiments are also performed with the purpose of measuring rates of metabolic reactions. These measurements reveal the contribution of secreted hormones to the formation of circulating compounds and urinary metabolites. The estimation of rates of fetal and placental production and exchange of hormones characterizes a class of in vivo quantitative studies performed with isotopically labeled hormones (radioactive or not). In addi tion, tracers are used to measure permeability and rates of reaction in in vitro systems, and to study the uptake of hormones by tissues, both in vivo and in vitro. The stability of the steroid nucleus carrying the isotopic label and the large number of reversible metabolic reactions in which steroids are involved, both facilitated and motivated the development of a sophisticated theoretical treat ment of tracer experiments in the field of endocrinology. Although the prac tical examples used to illustrate the concepts and calculations presented in this monograph involve labeled hormones, the theory is presented in a general symbolic manner and is applicable to other fields of investigation.

Author : Robert R. Wolfe
Publisher : Wiley-Liss
Page : 480 pages
File Size : 42,7 Mb
Release : 1992-04-09
Category : Medical
ISBN : 0471561312

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Radioactive and Stable Isotope Tracers in Biomedicine by Robert R. Wolfe Pdf

An up-to-date, detailed, authoritative overview regarding all aspects of the theory and practice involved in utilizing isotopic tracers for the in vivo study of human metabolic function. Presents specific technical information on performing tracer studies including isotope infusion, analysis and interpretation of data.

Author : Anonim
Publisher : Academic Press
Page : 402 pages
File Size : 54,7 Mb
Release : 2015-09-21
Category : Medical
ISBN : 9780128025345

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Metabolic Analysis Using Stable Isotopes by Anonim Pdf

Metabolic Analysis Using Stable Isotopes, the newest volume in Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in metabolic analysis using stable isotopes. Continues the legacy of this premier serial with quality chapters on metabolic analysis using stable isotopes Represents the newest volume in Methods in Enzymology, providing a premier serial with quality chapters authored by leaders in the field Ideal reference for those interested in the study of metabolism, metabolic tracing, isotopic labeling, and lipogenesis

Author : Claudio Cobelli,David Foster,Gianna Toffolo
Publisher : Springer Science & Business Media
Page : 455 pages
File Size : 47,6 Mb
Release : 2007-05-08
Category : Technology & Engineering
ISBN : 9780306468339

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Tracer Kinetics in Biomedical Research by Claudio Cobelli,David Foster,Gianna Toffolo Pdf

1. Introduction. 2. Fundamentals of Tracer Kinetics. 3. The Noncompartmental Model of Multipool Systems. 4. The Compartmental Model. 5. Identifiability of the Tracer Model. 6. Using the Tracer Model to Estimate Kinetic Parameters. 7. Compartmental Versus Noncompartmental Kinetic Parameters. 8. Parameter Estimation: Some Fundamentals of Regression Analysis. 9. Parameter Estimation in Noncompartmental Models. 10. Parameter Estimation in Compartmental Models. 11. Precursor-Product Models. Appendices. Index.

Author : Angelo D'Alessandro
Publisher : Humana
Page : 459 pages
File Size : 45,8 Mb
Release : 2020-06-06
Category : Science
ISBN : 1493992384

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High-Throughput Metabolomics by Angelo D'Alessandro Pdf

This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine.

Author : Frank Vanhaecke,Patrick Degryse
Publisher : John Wiley & Sons
Page : 565 pages
File Size : 43,8 Mb
Release : 2012-04-24
Category : Science
ISBN : 9783527650507

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Isotopic Analysis by Frank Vanhaecke,Patrick Degryse Pdf

Edited by two very well-known and respected scientists in the field, this excellent practical guide is the first to cover the fundamentals and a wide range of applications, as well as showing readers how to efficiently use this increasingly important technique. From the contents: * The Isotopic Composition of the Elements * Single-Collector ICP-MS * Multi-Collector ICP-MS * Advances in Laser Ablation - Multi-Collector ICP-MS * Correction for Instrumental Mass Discrimination in Isotope Ratio Determination with Multi-Collector ICP-MS * Reference Materials in Isotopic Analysis * Quality Control in Isotope Ratio Applications * Determination of Trace Elements and Elemental Species Using Isotope Dilution ICP-MS * Geochronological Dating * Application of Multi-Collector ICP-MS to Isotopic Analysis in Cosmochemistry * Establishing the Basis for Using Stable Isotope Ratios of Metals as Paleoredox Proxies * Isotopes as Tracers of Elements Across the Geosphere-Biosphere Interface * Archaeometric Applications * Forensics Applications * Nuclear Applications * The Use of Stable Isotope Techniques for Studying Mineral and Trace Element Metabolism in Humans * Isotopic Analysis via Multi-Collector ICP-MS in Elemental Speciation A must-have for newcomers as well as established scientists seeking an overview of isotopic analysis via ICP-MS.

Author : K. Schügerl,A.-P. Zeng
Publisher : Springer
Page : 274 pages
File Size : 47,6 Mb
Release : 2003-07-01
Category : Technology & Engineering
ISBN : 9783540457367

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Tools and Applications of Biochemical Engineering Science by K. Schügerl,A.-P. Zeng Pdf

This special volume "Tools and Applications of Biochemical Engineering Science" is dedicated to Professor Wolf-Dieter Deckwer on the occasion of his 60th bir- day. It was a great pleasure for me to act together with Professor Karl Schtigerl as volume editor and to present here a collection of 11 outstanding review articles written mainly by former students, associates, colleagues and friends of Wolf- Dieter Deckwer. The title of this special volume well reflects the research interests and sci- tific pursuit of Wolf-Dieter Deckwer during his more than 20 years’ work in the area of biochemical engineering, particularly during the last 15 years when he was the head of the Biochemical Engineering Division of GBF (German Nat- nal Research Center for Biotechnology). He has decisively pushed the devel- ment not only of "software tools" ranging from analytical means and mathe- tical models for monitoring and understanding cellular processes to gene expression systems for designing microorganisms, but also of "hardware tools" such as computer control systems, bioreaction and separation devices for eff- tively producing a variety of bioproducts on semi-production scale. New developments in some of these important tools in biochemical engineering are reviewed in articles included in this volume. Wolf-Dieter Deckwer was among the leading biochemical engineers who timely pointed out the necessity of applying these tools in an integrated manner for bioprocess development. By establishing "Integrated Bioprocess Development" as one of the GBF main - search topics as early as 1990 he also actively promoted this idea.

Author : C. Kendall,J.J. McDonnell
Publisher : Elsevier
Page : 870 pages
File Size : 51,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780080929156

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Isotope Tracers in Catchment Hydrology by C. Kendall,J.J. McDonnell Pdf

This book represents a new "earth systems" approach to catchments that encompasses the physical and biogeochemical interactions that control the hydrology and biogeochemistry of the system. The text provides a comprehensive treatment of the fundamentals of catchment hydrology, principles of isotope geochemistry, and the isotope variability in the hydrologic cycle -- but the main focus of the book is on case studies in isotope hydrology and isotope geochemistry that explore the applications of isotope techniques for investigating modern environmental problems. Isotope Tracers in Catchment Hydrology is the first synthesis of physical hydrology and isotope geochemistry with catchment focus, and is a valuable reference for professionals and students alike in the fields of hydrology, hydrochemistry, and environmental science. This important interdisciplinary text provides extensive guidelines for the application of isotope techniques for all investigatores facing the challenge of protecting precious water, soil, and ecological resources from the ever-increasing problems associated with population growth and environmental change, including those from urban development and agricultural land uses.

Author : Anonim
Publisher : Unknown
Page : 638 pages
File Size : 45,9 Mb
Release : 1979
Category : Biochemistry
ISBN : CORNELL:31924002026916

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Techniques in Metabolic Research by Anonim Pdf

Author : Shanmugasundaram Ganapathy-Kanniappan
Publisher : Springer Nature
Page : 76 pages
File Size : 54,5 Mb
Release : 2022-05-03
Category : Science
ISBN : 9783030997007

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Isotopic Tracer Techniques in Preclinical Research by Shanmugasundaram Ganapathy-Kanniappan Pdf

This book serves as a practical guide and a manual on the application of isotopic tracers in understanding the cellular processes (e.g. molecular interactions, protein synthesis) in growth, development, and disease conditions. The techniques described in the book have been carefully chosen to underscore the role and relevance of isotopic tracers in the identification of molecular targets and mechanisms, and preclinical validation of potential therapeutics. This book is intended to be a valuable resource to non-experts that may include students, researchers, educators/teachers, and others who have an interest to understand the application of isotopic tracers in research. Each chapter includes a background, an outline of the method and underlying principles, and a detailed step-wise protocol. To augment the clarity and intellectual grasp, the chapters also include relevant illustrations and study questions. More importantly, the book covers techniques involving radioactive as well as non-radioactive tracers to expand the scope of isotopes in preclinical research including basic and applied science.

Author : Brian Fry
Publisher : Springer Science & Business Media
Page : 308 pages
File Size : 40,7 Mb
Release : 2007-01-15
Category : Science
ISBN : 9780387337456

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Stable Isotope Ecology by Brian Fry Pdf

A solid introduction to stable isotopes that can also be used as an instructive review for more experienced researchers and professionals. The book approaches the use of isotopes from the perspective of ecological and biological research, but its concepts can be applied within other disciplines. A novel, step-by-step spreadsheet modeling approach is also presented for circulating tracers in any ecological system, including any favorite system an ecologist might dream up while sitting at a computer. The author’s humorous and lighthearted style painlessly imparts the principles of isotope ecology. The online material contains color illustrations, spreadsheet models, technical appendices, and problems and answers.