Transplantation Immunology Methods And Protocols

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Transplantation Immunology

Philip Hornick
Transplantation Immunology Book PDF

Author : Philip Hornick
Publisher : Springer Science & Business Media
Page : 430 pages
File Size : 55,6 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781597450492

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Transplantation Immunology by Philip Hornick Pdf

Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ. The authors present the latest techniques for HLA typing, detecting HLA antibodies, and monitoring T-cell response, and examine more specialized methods utilizing proteomics, laser dissection microscopy, and real-time polymerase chain reaction. The area of tolerance induction and reprogramming of the immune system is also covered, along with a discussion of up-to-date methods of organ preservation, of today's optimal immunosuppressive drug regimens, as well as the difficulty of mimicking chronic rejection in experimental models. Introductory chapters provide a theoretical update on current practices in renal, liver, islet, and lung transplantation and on the pathways of antigen presentation and chronic rejection.

Transplant Immunology

Xian C. Li,Anthony M. Jevnikar
Transplant Immunology Book PDF

Author : Xian C. Li,Anthony M. Jevnikar
Publisher : John Wiley & Sons
Page : 363 pages
File Size : 51,9 Mb
Release : 2015-11-16
Category : Medical
ISBN : 9780470658215

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Transplant Immunology by Xian C. Li,Anthony M. Jevnikar Pdf

With all the complex issues of acceptance or rejection of a transplanted organ, immunology is a key subject for all transplantation clinicians. During recent years, there has been an explosion of research and knowledge in this area. Produced in association with the American Society of Transplantation, and written by experts within the field, Transplant Immunology provides a comprehensive overview of the topic in relation to clinical transplantation. Starting with the basic functionality of the immune system, it then moves on to cover the very latest developments in immunosuppressive drugs and protocols, as well as a look at all emerging technologies in the field. Key chapters include: Transplant-related complications Immune responses to transplants Emerging issues in transplantation Biomarkers of Allograft rejection and tolerance T cells and the principles of immune responses In full colour throughout, over 100 outstanding diagrams support the text, all figures being fully downloadable via the book's companion website. The result is an essential tool for all those responsible for managing patients awaiting and undergoing organ transplantation, including transplant surgeons and clinicians, immunologists and researchers.

Transplantation Immunology

Andrea A. Zachary,Mary S. Leffell
Transplantation Immunology Book PDF

Author : Andrea A. Zachary,Mary S. Leffell
Publisher : Humana
Page : 0 pages
File Size : 46,8 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493960156

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Transplantation Immunology by Andrea A. Zachary,Mary S. Leffell Pdf

After decades of research in clinical transplantation, new techniques have been developed that permit a further understanding of the immune mechanisms underlying immune recognition of allografts and a more accurate and thorough evaluation of compatibility between donors and recipients. The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology. The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research. Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms, as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research.

Immunological Tolerance

Ashleigh S. Boyd
Immunological Tolerance Book PDF

Author : Ashleigh S. Boyd
Publisher : Humana Press
Page : 240 pages
File Size : 55,5 Mb
Release : 2019-01-17
Category : Medical
ISBN : 1493989367

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Immunological Tolerance by Ashleigh S. Boyd Pdf

This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Transplantation Immunology: Methods and Protocols

Kayla Streep
Transplantation Immunology Methods and Protocols Book PDF

Author : Kayla Streep
Publisher : Unknown
Page : 248 pages
File Size : 40,5 Mb
Release : 2020-09-22
Category : Medical
ISBN : 163241869X

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Transplantation Immunology: Methods and Protocols by Kayla Streep Pdf

Transplantation refers to the process of moving cells, tissues or organs from one part of the body to another, whether within the same person, or between a donor and a recipient. It is usually a life-saving intervention and is a major operation. However, the transplanted tissue or organ may be rejected by the immune system. Rejection is an adaptive immune response of the body that can occur due to the mechanisms of cellular immunity or humoral immunity. Different transplanted tissues favor different rejection mechanisms. Acute rejection occurs in all transplants unless immunosuppression has been achieved. An exception to this is transplantation between identical twins. Hyperacute rejection response sets in within minutes after a transplant, which if unattended may trigger a systemic inflammatory response syndrome. Rapid clumping, particularly the agglutination of red blood cells, is a common risk in kidney transplants. Chronic rejection can arise when there is a long-term loss of function in the transplanted organ. This loss in function arises due to fibrosis of the blood vessels of the transplanted tissues. This is responsible for the long-term morbidity witnessed in lung-transplant recipients. Transplant rejection can be lowered by using immunosuppressant drugs after transplantation and by prior determination of the molecular similitude between donor and recipient. This book provides comprehensive insights into the field of transplantation immunology. It presents researches and studies performed by experts across the globe on the methods and protocols of transplantation. It is a vital tool for all researching and studying this field.

Kidney Transplantation, Bioengineering, and Regeneration

Giuseppe Orlando,Giuseppe Remuzzi,David F. Williams
Kidney Transplantation Bioengineering and Regeneration Book PDF

Author : Giuseppe Orlando,Giuseppe Remuzzi,David F. Williams
Publisher : Academic Press
Page : 1252 pages
File Size : 52,5 Mb
Release : 2017-06-08
Category : Science
ISBN : 9780128018361

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Kidney Transplantation, Bioengineering, and Regeneration by Giuseppe Orlando,Giuseppe Remuzzi,David F. Williams Pdf

Kidney Transplantation, Bioengineering, and Regeneration: Kidney Transplantation in the Regenerative Medicine Era investigates how the field of regenerative medicine is changing the traditional premises of solid organ transplantation, specifically within the field of kidney transplantation. In Section 1, chapters illustrate the state of the art in kidney transplantation as well as the research behind the bioengineering and regeneration of kidney organoids for therapeutic renal replacement. In Section II, chapters catalog the technologies that are being developed and the methods that are being implemented to bioengineer or regenerate kidneys in order to restore function, while critically highlighting those technological advances which hold the most promise. The book thus encompasses clinical renal transplantation, tissue engineering, biomaterial sciences, stem cell biology, and developmental biology, as they are all applied to the kidney. Focuses on the synergy between renal organ transplantation and regenerative medicine, highlighting the advances within transplantation, bioengineering, regeneration, and repair Educates the transplant community on important regenerative medicine research pertinent to kidney transplantation Develops a shared language for clinicians, surgeons, and basic researchers to reach across the fields of transplantation and regenerative medicine, and facilitate more productive investigation and research Catalogs the technologies being developed and methods being implemented to bioengineer or regenerate kidneys to restore function

Complement Methods and Protocols

B. Paul Morgan
Complement Methods and Protocols Book PDF

Author : B. Paul Morgan
Publisher : Springer Science & Business Media
Page : 268 pages
File Size : 53,6 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781592590568

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Complement Methods and Protocols by B. Paul Morgan Pdf

The complement system, first described more than a century ago, was for many years the ugly duckling of the immunology world, but no more. Complement in recent years has blossomed into a fascinating and fast moving field of immediate relevance to clinical scientists in fields as diverse as transplantation biology, virology, and inflammation. Despite its emergence from the shadows, complement retains an unwarranted reputation for being “difficult.” This impression derives in large part from the superficially complicated nomenclature, a relic of the long and tortuous process of unraveling the system, of naming components in order of discovery rather than in a syst- atic manner. Once the barrier of nomenclature has been surmounted, then the true simplicity of the system becomes apparent. Complement comprises an activation system and a cytolytic system. The former has diverged to focus on complement to distinct targets—bacteria, - mune complexes, and others—so that texts now describe three activation pa- ways, closely related to one another, but each with some unique features. The cytolytic pathway is the same regardless of the activation process and kills cells by creating pores in the membrane. Complement plays an important role in killing bacteria and is essential for the proper handling of immune complexes. Problems occur when complement is activated in an inappropriate manner—the potent inflammation-inducing products of the cascade then cause unwanted tissue damage and destruction.

In Situ Hybridization Protocols

Ian A. Darby,Tim D. Hewitson
In Situ Hybridization Protocols Book PDF

Author : Ian A. Darby,Tim D. Hewitson
Publisher : Springer Science & Business Media
Page : 272 pages
File Size : 41,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450072

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In Situ Hybridization Protocols by Ian A. Darby,Tim D. Hewitson Pdf

The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.

MicroRNA Protocols

Shao-Yao Ying
MicroRNA Protocols Book PDF

Author : Shao-Yao Ying
Publisher : Springer Science & Business Media
Page : 365 pages
File Size : 48,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597451239

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MicroRNA Protocols by Shao-Yao Ying Pdf

MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.

Cytochrome P450 Protocols

Ian R. Phillips
Cytochrome P450 Protocols Book PDF

Author : Ian R. Phillips
Publisher : Springer Science & Business Media
Page : 355 pages
File Size : 41,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592599981

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Cytochrome P450 Protocols by Ian R. Phillips Pdf

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.

Embryonic Stem Cell Protocols

Kursad Turksen
Embryonic Stem Cell Protocols Book PDF

Author : Kursad Turksen
Publisher : Springer Science & Business Media
Page : 504 pages
File Size : 47,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450379

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Embryonic Stem Cell Protocols by Kursad Turksen Pdf

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Arabidopsis Protocols, 2nd Edition

Julio Salinas,Jose J. Sanchez-Serrano
Arabidopsis Protocols 2nd Edition Book PDF

Author : Julio Salinas,Jose J. Sanchez-Serrano
Publisher : Springer Science & Business Media
Page : 461 pages
File Size : 47,5 Mb
Release : 2008-02-04
Category : Nature
ISBN : 9781597450034

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Arabidopsis Protocols, 2nd Edition by Julio Salinas,Jose J. Sanchez-Serrano Pdf

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.

DNA Repair Protocols

Daryl S. Henderson
DNA Repair Protocols Book PDF

Author : Daryl S. Henderson
Publisher : Springer Science & Business Media
Page : 498 pages
File Size : 40,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592599738

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DNA Repair Protocols by Daryl S. Henderson Pdf

The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book’s thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in the first edition was dispensed with and chapters concerned with ionizing radiation damage and DNA strand breakage and repair were re- cated to near the front of the book. Finally, an abstract now heads each chapter.

Transmembrane Signaling Protocols

Hydar Ali,Haribabu Bodduluri
Transmembrane Signaling Protocols Book PDF

Author : Hydar Ali,Haribabu Bodduluri
Publisher : Springer Science & Business Media
Page : 354 pages
File Size : 52,8 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597450485

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Transmembrane Signaling Protocols by Hydar Ali,Haribabu Bodduluri Pdf

The previous edition of Transmembrane Signaling Protocols was published in 1998. Since then the human genome has been completely sequenced and new methods have been developed for the use of microarrays and proteomics to analyze global changes in gene expression and protein profiles. These advances have increased our ability to understand transmembrane signaling processes in much greater detail. They have also simultaneously enhanced our ability to determine the role of a large number of newly identified molecules in signaling events. In addition, novel video microscopy methods have been developed to image transmembrane signaling events in live cells in real time. In view of these major advances, it is time to update the previous edition. Because of the success of that volume, we have chosen to keep the essential character of the book intact. Introductory chapters from experts have been included to provide overall perspective and an overview of recent advances in signal transduction pathways. The individual chapters now include comp- hensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from tra- genic mice, as well as genomic and proteomic approaches to the analysis of transmembrane signaling events. We would like to express our deep gratitude to the coauthors of this publi- tion. We hope that Transmembrane Signaling Protocols, Second Edition will serve as a valuable resource for future progress in the study of signal transd- tion pathways.

Diagnostic Bacteriology Protocols

Louise O’Connor
Diagnostic Bacteriology Protocols Book PDF

Author : Louise O’Connor
Publisher : Springer Science & Business Media
Page : 226 pages
File Size : 46,7 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597451437

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Diagnostic Bacteriology Protocols by Louise O’Connor Pdf

The field of bacterial diagnostics has seen unprecedented advances in recent years. The increased need for accurate detection and identification of bacteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in laboratories around the world, there is now a growing trend toward the use of nucleic ac- based diagnostics and alternative biochemically and immunologically based formats. The ultimate goal of all diagnostic tests is the accurate detection, identification, or typing of microorganisms in samples of interest. Although the resulting information is of obvious use in the areas of patient management, animal health, and quality control, it is also of use in monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.