Yeast Protocols

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Yeast Protocols

Wei Xiao
Yeast Protocols Book PDF

Author : Wei Xiao
Publisher : Humana
Page : 0 pages
File Size : 45,6 Mb
Release : 2016-09-17
Category : Science
ISBN : 1493942778

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Yeast Protocols by Wei Xiao Pdf

Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.

Yeast Protocols

Ivor Howell Evans
Yeast Protocols Book PDF

Author : Ivor Howell Evans
Publisher : Springer
Page : 433 pages
File Size : 49,6 Mb
Release : 1996
Category : Medical
ISBN : 0896033198

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Yeast Protocols by Ivor Howell Evans Pdf

Yeast Protocols contains many key techniques for studying the biology of yeasts at both the cellular and molecular levels. Working primarily from Saccharomyces cerevisiae, the expert contributors explain step-by-step how to successfully isolate, identify, and culture yeasts; the secrets of meiotic mapping; how to use PFGE in karyotyping and gene localization; the methods for purification and analysis of various cell components; and the construction and exploitation of genomic DNA clone banks. They also cover the latest methods for chromosome engineering, insertional mutagenesis by Ty elements, mRNA abundance and half-life measurements, the use of reporter gene systems, genotoxicity testing, and more. Yeast Protocols follows the widely applauded Humana Methods in Molecular Biology style: brief introductions putting the particular method in context, comprehensive lists of materials, cookbook style instructions, and troubleshooting notes to avoid common pitfalls and solve problems. The techniques can be used with confidence and success by both inexperienced newcomers and established researchers.

Yeast Genetics

Jeffrey S. Smith,Daniel J. Burke
Yeast Genetics Book PDF

Author : Jeffrey S. Smith,Daniel J. Burke
Publisher : Humana
Page : 0 pages
File Size : 44,8 Mb
Release : 2016-09-17
Category : Science
ISBN : 1493951823

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Yeast Genetics by Jeffrey S. Smith,Daniel J. Burke Pdf

Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.

Yeast Protocols

Wei Xiao
Yeast Protocols Book PDF

Author : Wei Xiao
Publisher : Springer Science & Business Media
Page : 389 pages
File Size : 47,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592599585

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Yeast Protocols by Wei Xiao Pdf

In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.

Recombinant Protein Production in Yeast

Roslyn M. Bill
Recombinant Protein Production in Yeast Book PDF

Author : Roslyn M. Bill
Publisher : Humana Press
Page : 262 pages
File Size : 46,5 Mb
Release : 2016-05-01
Category : Medical
ISBN : 1493958712

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Recombinant Protein Production in Yeast by Roslyn M. Bill Pdf

This book reviews preparation of expression vectors, generation of high-yielding clones, scale-up, disruption of yeast cells to enable isolation of recombinant protein prior to purification and more, in the popular Methods in Molecular Biology format."

Yeast Metabolic Engineering

Valeria Mapelli
Yeast Metabolic Engineering Book PDF

Author : Valeria Mapelli
Publisher : Humana
Page : 0 pages
File Size : 47,5 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493952862

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Yeast Metabolic Engineering by Valeria Mapelli Pdf

Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.

Yeast Functional Genomics

Frédéric Devaux
Yeast Functional Genomics Book PDF

Author : Frédéric Devaux
Publisher : Humana
Page : 0 pages
File Size : 44,7 Mb
Release : 2015-10-20
Category : Science
ISBN : 1493930788

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Yeast Functional Genomics by Frédéric Devaux Pdf

This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Functional Genomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

PCR Protocols

John M. S. Bartlett,David Stirling
PCR Protocols Book PDF

Author : John M. S. Bartlett,David Stirling
Publisher : Springer Science & Business Media
Page : 1083 pages
File Size : 43,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592593842

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PCR Protocols by John M. S. Bartlett,David Stirling Pdf

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Yeast Systems Biology

Juan I. Castrillo,Stephen G. Oliver
Yeast Systems Biology Book PDF

Author : Juan I. Castrillo,Stephen G. Oliver
Publisher : Humana Press
Page : 0 pages
File Size : 53,9 Mb
Release : 2011-08-23
Category : Science
ISBN : 1617791725

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Yeast Systems Biology by Juan I. Castrillo,Stephen G. Oliver Pdf

Systems Biology aims at deciphering the genotype-phenotype relationships at the levels of genes, transcripts (RNAs), peptides, proteins, metabolites, and environmental factors participating in complex cellular networks in order to reveal the mechanisms and principles governing the behavior of complex biological systems. Yeast Systems Biology: Methods and Protocols presents an up-to-date view of the optimal characteristics of the yeast Saccharomyces cerevisiae as a model eukaryote, perspective on the latest experimental and computational techniques for systems biology studies, most of which were first designed for and validated in yeast, and selected examples of yeast systems biology studies and their applications in biotechnology and medicine. These experiments under controlled conditions can uncover the complexity and interplay of biological networks with their dynamics, basic principles of internal organization, and balanced orchestrated functions between organelles in direct interaction with the environment as well as the characterization of short and long-term effects of perturbations and dysregulation of networks that may illuminate the origin of complex human diseases. Written for the highly successful Methods in Molecular BiologyTM series, this volume contains the kind of detailed description and implementation advice that is crucial for getting optimal results. Practical and cutting-edge, Yeast Systems Biology: Methods and Protocols serves researchers interested in comprehensive systems biology strategies in well-defined model systems with specific objectives as well as a better knowledge of the latest post-genomic strategies at all ‘omic levels and computational approaches towards analysis, integration, and modeling of biological systems, from single-celled organisms to higher eukaryotes.

Methods in Yeast Genetics

David C. Amberg,Dan Burke,Jeffrey N. Strathern
Methods in Yeast Genetics Book PDF

Author : David C. Amberg,Dan Burke,Jeffrey N. Strathern
Publisher : CSHL Press
Page : 250 pages
File Size : 48,9 Mb
Release : 2005
Category : Genetics
ISBN : 9780879697280

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Methods in Yeast Genetics by David C. Amberg,Dan Burke,Jeffrey N. Strathern Pdf

"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.

Food Microbiology Protocols

John F. T. Spencer,Alicia L. Ragout de Spencer
Food Microbiology Protocols Book PDF

Author : John F. T. Spencer,Alicia L. Ragout de Spencer
Publisher : Springer Science & Business Media
Page : 482 pages
File Size : 46,8 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590292

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Food Microbiology Protocols by John F. T. Spencer,Alicia L. Ragout de Spencer Pdf

Two of the recent books in the Methods in Molecular Biology series, Yeast Protocols and Pichia Protocols, have been narrowly focused on yeasts and, in the latter case, particular species of yeasts. Food Microbiology Pro- cols, of necessity, covers a very wide range of microorganisms. Our book treats four categories of microorganisms affecting foods: (1) Spoilage organisms; (2) pathogens; (3) microorganisms in fermented foods; and (4) microorganisms p- ducing metabolites that affect the flavor or nutritive value of foods. Detailed information is given on each of these categories. There are several chapters devoted to the microorganisms associated with fermented foods: these are of increasing importance in food microbiology, and include one bacteriophage that kills the lactic acid bacteria involved in the manufacture of different foods—cottage cheese, yogurt, sauerkraut, and many others. The other nine chapters give procedures for the maintenance of lactic acid bacteria, the isolation of plasmid and genomic DNA from species of Lac- bacillus, determination of the proteolytic activity of lactic acid bacteria, det- mination of bacteriocins, and other important topics.

Yeast Surface Display

Bin Liu
Yeast Surface Display Book PDF

Author : Bin Liu
Publisher : Humana
Page : 0 pages
File Size : 44,7 Mb
Release : 2016-10-09
Category : Science
ISBN : 1493946676

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Yeast Surface Display by Bin Liu Pdf

In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers. ​

Candida Albicans

Ronald L. Cihlar,Richard A. Calderone
Candida Albicans Book PDF

Author : Ronald L. Cihlar,Richard A. Calderone
Publisher : Humana Press
Page : 0 pages
File Size : 48,7 Mb
Release : 2009-02-05
Category : Science
ISBN : 1588297608

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Candida Albicans by Ronald L. Cihlar,Richard A. Calderone Pdf

Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiasis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful "Methods in Molecular BiologyTM" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology.

Methods in Yeast Genetics and Genomics

Maitreya J. Dunham,Marc R. Gartenberg,Grant W. Brown
Methods in Yeast Genetics and Genomics Book PDF

Author : Maitreya J. Dunham,Marc R. Gartenberg,Grant W. Brown
Publisher : Unknown
Page : 0 pages
File Size : 43,5 Mb
Release : 2015
Category : Cytogenetik
ISBN : 162182134X

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Methods in Yeast Genetics and Genomics by Maitreya J. Dunham,Marc R. Gartenberg,Grant W. Brown Pdf

Methods in Yeast Genetics is a course that has been offered annually at Cold Spring Harbor Laboratory for the last 45 years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Since the last edition of the manual was published (2005), revolutionary advances in genomics, proteomics, and imaging technologies have had a significant impact on the field. The 11 experiments included in this manual provide a foundation of methods for any modern-day yeast lab. These methods emphasize combinations of classical and modern genetic approaches, including isolation and characterization of mutants, two-hybrid analysis, tetrad analysis, complementation, and recombination. Also covered are molecular genetic techniques for genome engineering. Additional experiments introduce fundamental techniques in yeast genomics, including both performance and interpretation of Synthetic Genetic Array analysis, multiplexed whole genome and barcode sequencing, and comparative genomic hybridization to DNA arrays. Comparative genomics is introduced using different yeast strains to study natural variation, evolution, and quantitative traits. This manual covers the full repertoire of genetic approaches needed to dissect complex biological problems in the yeast Saccharomyces cerevisiae.

Pichia Protocols

James M Cregg
Pichia Protocols Book PDF

Author : James M Cregg
Publisher : Springer Science & Business Media
Page : 553 pages
File Size : 40,9 Mb
Release : 2007-08-08
Category : Science
ISBN : 9781588294296

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Pichia Protocols by James M Cregg Pdf

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.