Advanced Mass Spectrometry Based Analytical Separation Techniques For Probing The Polar Metabolome

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Advanced Mass Spectrometry-based Analytical Separation Techniques for Probing the Polar Metabolome

Rawi Ramautar
Advanced Mass Spectrometry based Analytical Separation Techniques for Probing the Polar Metabolome Book PDF

Author : Rawi Ramautar
Publisher : Royal Society of Chemistry
Page : 367 pages
File Size : 40,8 Mb
Release : 2021-07-13
Category : Science
ISBN : 9781839163531

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Advanced Mass Spectrometry-based Analytical Separation Techniques for Probing the Polar Metabolome by Rawi Ramautar Pdf

The efficient analysis of polar and charged metabolites in biological samples remains a huge challenge in the field of metabolomics. Over the past years, novel mass spectrometry-based analytical tools have been developed to enable the sensitive and efficient profiling of polar ionogenic metabolites in various biological samples. This book gives the reader a comprehensive overview of these recent technological developments. Topics covered include the use of chemical labelling strategies for allowing the analysis of polar metabolites using reversed-phase liquid chromatography–mass spectrometry (RPLC-MS) and the latest methodological developments in RPLC-MS, hydrophilic interaction liquid chromatography (HILIC)-MS and ion-pair LC-MS approaches. Attention is also paid to developments in nano-LC-MS and capillary electrophoresis–mass spectrometry methods specifically for profiling polar metabolites in small volume biological samples. The utility of ion-mobility MS and NMR spectroscopy will also be outlined. Sample preparation is the key part in the analytical workflow employed for metabolomics. Therefore, ample emphasis will be given on recent solid-phase extraction and solid-phase micro-extraction methods. Finally, analytical techniques for chiral metabolic profiling will also be considered. Discussing the state-of-the-art of the proposed topics in one single book for probing the polar metabolome, using relevant examples, is unique and needed in the metabolomics field. This book has relevance and appeal to an international audience of analytical and biomedical researchers in industry and academia.

Advanced Mass Spectrometry-based Analytical Separation Techniques for Probing the Polar Metabolome

Rawi Ramautar
Advanced Mass Spectrometry based Analytical Separation Techniques for Probing the Polar Metabolome Book PDF

Author : Rawi Ramautar
Publisher : Royal Society of Chemistry
Page : 367 pages
File Size : 53,7 Mb
Release : 2021-07-21
Category : Science
ISBN : 9781839161636

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Advanced Mass Spectrometry-based Analytical Separation Techniques for Probing the Polar Metabolome by Rawi Ramautar Pdf

Discussing the state-of-the-art of the proposed topics in one single book for probing the polar metabolome, using relevant examples, is unique and needed in the metabolomics field.

Liquid Chromatography

Salvatore Fanali,Bezhan Chankvetadze,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola
Liquid Chromatography Book PDF

Author : Salvatore Fanali,Bezhan Chankvetadze,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola
Publisher : Elsevier
Page : 1086 pages
File Size : 40,8 Mb
Release : 2023-04-20
Category : Science
ISBN : 9780323983006

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Liquid Chromatography by Salvatore Fanali,Bezhan Chankvetadze,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola Pdf

Liquid Chromatography: Applications, Third Edition delivers a single source of authoritative information on all aspects of the practice of modern liquid chromatography. The text gives those working in academia and industry the opportunity to learn, refresh, and deepen their understanding of the field by covering basic and advanced theoretical concepts, recognition mechanisms, conventional and advanced instrumentation, method development, data analysis, and more. This third edition addresses new developments in the field with updated chapters from expert researchers. The book is a valuable reference for research scientists, teachers, university students, industry professionals in research and development, and quality control managers. Emphasizes the integration of chromatographic methods and sample preparation Provides important data related to complex matrices, sample preparation, and data handling Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Offers comprehensive updates to all chapters Adds new chapters on selection of liquid chromatographic mode, proteomics, doping analysis, analysis of microplastics, and analysis of pharmaceutically and biologically relevant isoforms

Metabolome Analysis

Silas G. Villas-Boas,Jens Nielsen,Jorn Smedsgaard,Michael A. E. Hansen,Ute Roessner-Tunali
Metabolome Analysis Book PDF

Author : Silas G. Villas-Boas,Jens Nielsen,Jorn Smedsgaard,Michael A. E. Hansen,Ute Roessner-Tunali
Publisher : John Wiley & Sons
Page : 339 pages
File Size : 41,6 Mb
Release : 2007-02-16
Category : Science
ISBN : 9780470105504

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Metabolome Analysis by Silas G. Villas-Boas,Jens Nielsen,Jorn Smedsgaard,Michael A. E. Hansen,Ute Roessner-Tunali Pdf

Providing information on the main approaches for the analysis of metabolites, this textbook: Covers basic methodologies in sample preparation and separation techniques, as well as the most recent techniques of mass spectrometry. Differentiates between primary and secondary metabolites. Includes four chapters discussing successful metabolome studies of different organisms. Highlights the analytical challenges of studying metabolites. Illustrates applications of metabolome analysis through the use of case studies.

Separation Techniques Applied to Omics Sciences

Ana Valéria Colnaghi Simionato
Separation Techniques Applied to Omics Sciences Book PDF

Author : Ana Valéria Colnaghi Simionato
Publisher : Springer Nature
Page : 277 pages
File Size : 55,5 Mb
Release : 2021-10-09
Category : Science
ISBN : 9783030772529

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Separation Techniques Applied to Omics Sciences by Ana Valéria Colnaghi Simionato Pdf

This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.

Mass Spectrometry-Based Metabolomics

Sastia Prama Putri,Eiichiro Fukusaki
Mass Spectrometry Based Metabolomics Book PDF

Author : Sastia Prama Putri,Eiichiro Fukusaki
Publisher : CRC Press
Page : 298 pages
File Size : 46,9 Mb
Release : 2014-11-19
Category : Science
ISBN : 9781482223767

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Mass Spectrometry-Based Metabolomics by Sastia Prama Putri,Eiichiro Fukusaki Pdf

Mass Spectrometry-Based Metabolomics: A Practical Guide is a simple, step-by-step reference for profiling metabolites in a target organism. It discusses optimization of sample preparation for urine, serum, blood, tissue, food, and plant and animal cell samples. Encompassing three different technical fields—biology, analytical chemistry, and informatics— mass spectrometry-based metabolomics can be challenging for biologists without special training in quantitative mass spectrometry. This book is designed to overcome this limitation by providing researchers with the knowledge they need to use metabolomics technology in their respective disciplines. The book summarizes all steps in metabolomics research, from experimental design to sample preparation, analytical procedures, and data analysis. Case studies are presented for easy understanding of the metabolomics workflow and its practical applications in different research fields. The book includes an in-house library and built-in software so that those new to the field can begin to analyze real data samples. In addition to being an excellent introductory text, the book also contains the latest advancements in this emerging field and can thus be a useful reference for metabolomics specialists.

Sample Preparation in Metabolomics

Julia Kuligowski,Guillermo Quintás
Sample Preparation in Metabolomics Book PDF

Author : Julia Kuligowski,Guillermo Quintás
Publisher : MDPI
Page : 206 pages
File Size : 45,6 Mb
Release : 2021-04-07
Category : Medical
ISBN : 9783039438136

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Sample Preparation in Metabolomics by Julia Kuligowski,Guillermo Quintás Pdf

Metabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with the selected analytical approach and the study aim, sample collection and preparation are critical steps that must be carefully selected and optimized to generate high-quality metabolomic data. This book showcases some of the most recent developments in the field of sample preparation for metabolomics studies. Novel technologies presented include electromembrane extraction of polar metabolites from plasma samples and guidelines for the preparation of biospecimens for the analysis with high-resolution μ magic-angle spinning nuclear magnetic resonance (HR-μMAS NMR). In the following chapters, the spotlight is on sample preparation approaches that have been optimized for diverse bioanalytical applications, including the analysis of cell lines, bacteria, single spheroids, extracellular vesicles, human milk, plant natural products and forest trees.

Capillary Electrophoresis–Mass Spectrometry for Metabolomics

Rawi Ramautar
Capillary Electrophoresis Mass Spectrometry for Metabolomics Book PDF

Author : Rawi Ramautar
Publisher : Royal Society of Chemistry
Page : 300 pages
File Size : 42,8 Mb
Release : 2018-07-13
Category : Science
ISBN : 9781788011044

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Capillary Electrophoresis–Mass Spectrometry for Metabolomics by Rawi Ramautar Pdf

Capillary electrophoresis–mass spectrometry (CE-MS) has become a very useful analytical technique for the profiling of highly polar and charged metabolites in biological samples. In this book, the unique features of CE-MS for metabolomics studies are highlighted including CE separation modes, capillary coatings and practical aspects of CE-MS coupling alongside a comprehensive overview of recent technological developments and applications. CE-MS can be considered a relatively new technique in the field of metabolomics and it is therefore important to inform the scientific community about the possibilities of advanced CE-MS approaches for metabolomics studies. This book outlines the potential of this technique for researchers working in metabolomics, bioanalytics and biomarker analysis.

Methodologies for Metabolomics

Norbert W. Lutz,Jonathan V. Sweedler,Ron A. Wevers
Methodologies for Metabolomics Book PDF

Author : Norbert W. Lutz,Jonathan V. Sweedler,Ron A. Wevers
Publisher : Cambridge University Press
Page : 128 pages
File Size : 47,6 Mb
Release : 2013-01-21
Category : Medical
ISBN : 9781139619059

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Methodologies for Metabolomics by Norbert W. Lutz,Jonathan V. Sweedler,Ron A. Wevers Pdf

Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

Chromatographic Methods in Metabolomics

Tuulia Hyotylainen,Susanne Wiedmer
Chromatographic Methods in Metabolomics Book PDF

Author : Tuulia Hyotylainen,Susanne Wiedmer
Publisher : Royal Society of Chemistry
Page : 258 pages
File Size : 50,5 Mb
Release : 2013
Category : Science
ISBN : 9781849736077

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Chromatographic Methods in Metabolomics by Tuulia Hyotylainen,Susanne Wiedmer Pdf

Intends to present chromatographic techniques in metabolomics in a fundamental way. This book covers the chromatographic techniques, such as liquid chromatography, gas chromatography, comprehensive two-dimensional gas chromatography and electrochromatographic techniques utilised in metabolomics.

Foodomics

Alejandro Cifuentes
Foodomics Book PDF

Author : Alejandro Cifuentes
Publisher : John Wiley & Sons
Page : 631 pages
File Size : 40,6 Mb
Release : 2013-02-05
Category : Medical
ISBN : 9781118537350

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Foodomics by Alejandro Cifuentes Pdf

Provides the latest "-omics" tools to advance the study of food and nutrition The rapidly emerging field of foodomics examines food and nutrition by applying advanced "-omics" technologies in order to improve people's health, well-being, and knowledge. Using tools from genomics, transcriptomics, epigenomics, proteomics, and metabolomics, foodomics offers researchers new analytical approaches to solve a myriad of current challenges in food and nutrition science. This book presents the fundamentals of foodomics, exploring the use of advanced mass spectrometry techniques in food science and nutrition in the post-genomic era. The first chapter of the book offers an overview of foodomics principles and applications. Next, the book covers: Modern instruments and methods of proteomics, including the study and characterization of food quality, antioxidant food supplements, and food allergens Advanced mass spectrometry-based methods to study transgenic foods and the microbial metabolome Mass spectrometry-based metabolomics in nutrition and health research Foodomics' impact on our current understanding of micronutrients (phenolic compounds and folates), optimal nutrition, and personalized nutrition and diet related diseases Principles and practices of lipidomics and green foodomics Use of chemometrics in mass spectrometry and foodomics The final chapter of Foodomics explores the potential of systems biology approaches in food and nutrition research. All the chapters conclude with references to the primary literature, enabling readers to explore individual topics in greater depth. With contributions from a team of leading pioneers in foodomics, this book enables students and professionals in food science and nutrition to take advantage of the latest tools to advance their research and open up new areas of food and nutrition investigation.

Metabolomics

Wolfram Weckwerth
Metabolomics Book PDF

Author : Wolfram Weckwerth
Publisher : Springer Science & Business Media
Page : 290 pages
File Size : 48,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597452441

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Metabolomics by Wolfram Weckwerth Pdf

Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Fundamentals of Advanced Omics Technologies: From Genes to Metabolites

Anonim
Fundamentals of Advanced Omics Technologies From Genes to Metabolites Book PDF

Author : Anonim
Publisher : Newnes
Page : 490 pages
File Size : 49,5 Mb
Release : 2014-02-14
Category : Science
ISBN : 9780444626707

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Fundamentals of Advanced Omics Technologies: From Genes to Metabolites by Anonim Pdf

Fundamentals of Advanced Omics Technologies: From Genes to Metabolites covers the fundamental aspects of the new instrumental and methodological developments in omics technologies, including those related to genomics, transcriptomics, epigenetics, proteomics and metabolomics, as well as other omics approaches such as glycomics, peptidomics and foodomics. The principal applications are presented in the following complementary volume. The chapters discuss in detail omics technologies, DNA microarray analysis, next-generation sequencing technologies, genome-wide analysis of methylation and histone modifications, emerging nanotechniques in proteomics, imaging mass spectrometry in proteomics, recent quantitative proteomics approaches, and advances in high-resolution NMR-based metabolomics, as well as MS-based non-targeted metabolomics and metabolome analysis by CE-MS, global glycomics analyses, foodomics, and high resolution analytical tools for quantitative peptidomics. Key aspects related to chemometrics, bioinformatics, data treatment, data integration and systems biology, deep-sequencing data analysis, statistical approaches for the analysis of microarray data, the integration of transcriptome and metabolome data and computational approaches for visualization and integration of omics data are also covered. Covers the latest advances in instrumentation, experimental design, sample preparation, and data analysis Provides thorough explanations and descriptions of specific omics technologies Describes advanced tools and methodologies for data pretreatment, storage, curation and analysis, as well as data integration

Mass Spectrometry for Metabolomics

Raúl González-Domínguez
Mass Spectrometry for Metabolomics Book PDF

Author : Raúl González-Domínguez
Publisher : Springer Nature
Page : 277 pages
File Size : 46,8 Mb
Release : 2022-09-24
Category : Science
ISBN : 9781071626993

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Mass Spectrometry for Metabolomics by Raúl González-Domínguez Pdf

This detailed volume provides a comprehensive overview of state-of-the-art metabolomics methods based on mass spectrometry (MS), and their application in food, nutrition, and biomedical research. The chapters assembled herein cover hot topics related to sample preparation, chromatographic and electrophoretic separation, MS-based analysis, as well as data processing and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry for Metabolomics serves as a timely guide for chemists, biochemists, biologists, nutritionists, clinicians, and other experts working in the growing and exciting field of metabolomics.

Applications of Advanced Omics Technologies: From Genes to Metabolites

Anonim
Applications of Advanced Omics Technologies From Genes to Metabolites Book PDF

Author : Anonim
Publisher : Elsevier
Page : 496 pages
File Size : 51,5 Mb
Release : 2014-05-08
Category : Science
ISBN : 9780444626691

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Applications of Advanced Omics Technologies: From Genes to Metabolites by Anonim Pdf

The book contains contributions concerning the application of the new instrumental and methodological developments in omics technologies, including those related to Genomics, Transcriptomics, Proteomics, Peptidomics and Metabolomics, Lipidomics and Foodomics. The16 chapters discuss in detail: innovative applications of functional gene microarrays for profiling microbial communities, microRNA profiling, novel genotyping applications using microarray technology in cancer research, next-generation sequencing applied to the study of human microbiome, emerging RNA-SEQ applications in food science, recent progress in plant proteomics, applications of gel-free proteomic approaches, the challenges and applications of proteomics tools for food authenticity, the role of salivary peptidomics in clinical applications, metabolomic approaches to the study of degenerative, cardiovascular and renal diseases, and neonatal medicine. Also covered are other omics applications such as profiling of genetically modified organisms, the fundamentals, applications and challenges of foodomics, and MS-based lipidomics. Moreover, this volume includes relevant and updated aspects on bioinformatics, data treatment, data integration and systems biology. This book complements the previous volume "Fundamentals of Advanced Omics Technologies: New Advances from Genes to Metabolites" that covered the fundamental aspects of these new omics technologies. Describes the latest applications of omics technologies Provides an excellent reference for applications of advanced omics techniques Includes advanced tools and methodologies for dealing with the data generated