Advancements Of Mass Spectrometry In Biomedical Research

Author : Alisa G. Woods,Costel C. Darie
Publisher : Springer
Page : 813 pages
File Size : 44,6 Mb
Release : 2019-07-25
Category : Science
ISBN : 9783030159504

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Advancements of Mass Spectrometry in Biomedical Research by Alisa G. Woods,Costel C. Darie Pdf

This volume explores the use of mass spectrometry for biomedical applications. Chapters focus on specific therapeutic areas such as oncology, infectious disease, and psychiatry. Additional chapters focus on methodology, technologies and instrumentation, as well as on analysis of protein-protein interactions, protein quantitation, and protein post-translational modifications. Various omics fields such as proteomics, metabolomics, glycomics, lipidomics, and adductomics are also covered. Applications of mass spectrometry in biotechnological and pharmaceutical industry are also discussed. This volume provides readers with a comprehensive and informative manual that will allow them to appreciate mass spectrometry and proteomic research, but also to initiate and improve their own work. This book acts as a technical guide as well as a conceptual guide to the newest information in this exciting field.

Author : Simon J. Gaskell
Publisher : Unknown
Page : 508 pages
File Size : 55,7 Mb
Release : 1986-01-01
Category : Electronic
ISBN : 0608009954

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Mass Spectrometry in Biomedical Research by Simon J. Gaskell Pdf

Author : Alisa G. Woods,Costel C. Darie
Publisher : Springer
Page : 0 pages
File Size : 51,5 Mb
Release : 2016-09-24
Category : Science
ISBN : 3319378929

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Advancements of Mass Spectrometry in Biomedical Research by Alisa G. Woods,Costel C. Darie Pdf

This volume explores the use of mass spectrometry for biomedical applications. Chapters focus on specific therapeutic areas such as oncology, infectious disease and psychiatry. Additional chapters focus on methodology as well as new technologies and instrumentation. This volume provides readers with a comprehensive and informative manual that will allow them to appreciate mass spectrometry and proteomic research but also to initiate and improve their own work. Thus the book acts as a technical guide but also a conceptual guide to the newest information in this exciting field. Mass spectrometry is the central tool used in proteomic research today and is rapidly becoming indispensable to the biomedical scientist. With the completion of the human genome project and the genomic revolution, the proteomic revolution has followed closely behind. Understanding the human proteome has become critical to basic and clinical biomedical research and holds the promise of providing comprehensive understanding of human physiological processes. In addition, proteomics and mass spectrometry are bringing unprecedented biomarker discovery and are helping to personalize medicine.

Author : A.L. Burlingame,Steven A. Carr,Michael A. Baldwin
Publisher : Springer Science & Business Media
Page : 576 pages
File Size : 49,9 Mb
Release : 1999-09-14
Category : Science
ISBN : 9781592597192

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Mass Spectrometry in Biology & Medicine by A.L. Burlingame,Steven A. Carr,Michael A. Baldwin Pdf

Leading practitioners detail revolutionary new spectrometric techniques for the identification and covalent structural characterization of macromolecules, proteins, glycoconjugates, and nucleic acids. Based on the Fourth International Symposium on Mass Spectrometry in the Health and Life Sciences held in San Francisco in 1998, this invaluable book contains tested strategies for solving many significant biomedical research problems. The techniques use mass spectrometry, automated computer processing of spectral information, and gene, protein, and EST databases for genomic and proteomic correlations. Mass Spectrometry in Biology and Medicine offers a unique opportunity to explore and apply these new techniques of mass spectrometry that are revolutionizing the identification and structural characterization of proteins, carbohydrates, and nucleic acids.

Author : Alberto Frigerio,Neal Castagnoli
Publisher : Halsted Press
Page : 616 pages
File Size : 40,5 Mb
Release : 1976
Category : Science
ISBN : UOM:39015003768275

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Advances in Mass Spectrometry in Biochemistry and Medicine by Alberto Frigerio,Neal Castagnoli Pdf

Author : Dominic M. Desiderio
Publisher : Springer Science & Business Media
Page : 360 pages
File Size : 45,5 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781489911735

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Mass Spectrometry by Dominic M. Desiderio Pdf

In response to the growing use of mass spectrometry in the clinical and biomedical fields, this book collects recent research involving electrospray ionization, neuronal systems, and structural modifications of proteins. The significant advances in instrumentation, methodology, experimentation presented herein will serve to expand the current concept of clinical mass spectrometry.

Author : Mary S. Lipton,Ljiljana Paša-Tolic
Publisher : Humana Press
Page : 470 pages
File Size : 54,6 Mb
Release : 2008-11-01
Category : Science
ISBN : 160761104X

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Mass Spectrometry of Proteins and Peptides by Mary S. Lipton,Ljiljana Paša-Tolic Pdf

When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach – referred to as shotgun proteomics – is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.

Author : Alexander R. Ivanov,Alexander V. Lazarev
Publisher : Springer Science & Business Media
Page : 1081 pages
File Size : 42,8 Mb
Release : 2011-06-15
Category : Science
ISBN : 9789400708280

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Sample Preparation in Biological Mass Spectrometry by Alexander R. Ivanov,Alexander V. Lazarev Pdf

The aim of this book is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis. With the latest inventions and introduction of highly sophisticated mass spectrometry equipment sample preparation becomes an extremely important bottleneck of biomedical analysis. We have a goal of giving the reader several successful examples of sample preparation, development and optimization, leading to the success in analytical steps and proper conclusions made at the end of the day. This book is structured as a compilation of contributed chapters ranging from protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.

Author : Gyorgy Marko-Varga,Peter Oroszlan
Publisher : Elsevier
Page : 294 pages
File Size : 40,6 Mb
Release : 2003-07-02
Category : Science
ISBN : 9780080530840

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Emerging Technologies in Protein and Genomic Material Analysis by Gyorgy Marko-Varga,Peter Oroszlan Pdf

It is widely recognized that analytical technologies and techniques are playing a pioneering role in a range of today's foremost challenging scientific endeavours, including especially biological and biomedical research. Worthy of mention, for example, are the role that high performance separation techniques played in mapping the human genome and the pioneering work done within mass spectrometry. It is also apparent that state-of-the-art pharmaceutical and biomedical research is the major driving force of the development of new analytical techniques. Advancements in genomics research has provided the opportunity for a call for new drug targets for new technologies, which has speeded up drug discovery and helped to counteract the trend towards inflation of R&D costs. This book has been designed to be a reference covering a wide range of protein and genomic material analysis techniques. Emerging developments are presented with applications where relevant, and biological examples are included. It was developed to meet the ever growing need for a comprehensive and balanced text on an analytical technique which has generated a tremendous amount of interest in recent years. In addition, this book also serves as a modern textbook for advanced undergraduate and graduate courses in various disciplines including chemistry, biology and pharmacy. Authors of the individual chapters are recognized champions of their individual research disciplines and also represent contemporary major research centres in this field. · Contains state-of-the-art knowledge of the field and detailed descriptions of new technologies · Provides examples of relevant applications and case studies · Contributing authors are leading scientists in their own respective research fields

Author : Thiago Verano-Braga
Publisher : Springer Nature
Page : 273 pages
File Size : 40,7 Mb
Release : 2024-05-20
Category : Electronic
ISBN : 9783031506246

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Mass Spectrometry-Based Approaches for Treating Human Diseases and Diagnostics by Thiago Verano-Braga Pdf

Author : Eram Sharmin,Fahmina Zafar
Publisher : BoD – Books on Demand
Page : 242 pages
File Size : 49,6 Mb
Release : 2017-12-06
Category : Science
ISBN : 9789535136279

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Spectroscopic Analyses by Eram Sharmin,Fahmina Zafar Pdf

The book presents developments and applications of these methods, such as NMR, mass, and others, including their applications in pharmaceutical and biomedical analyses. The book is divided into two sections. The first section covers spectroscopic methods, their applications, and their significance as characterization tools; the second section is dedicated to the applications of spectrophotometric methods in pharmaceutical and biomedical analyses. This book would be useful for students, scholars, and scientists engaged in synthesis, analyses, and applications of materials/polymers.

Author : Alexander R. Ivanov,Alexander V. Lazarev
Publisher : Springer
Page : 1089 pages
File Size : 41,9 Mb
Release : 2011-06-17
Category : Science
ISBN : 9400707584

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Sample Preparation in Biological Mass Spectrometry by Alexander R. Ivanov,Alexander V. Lazarev Pdf

The aim of this book is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis. With the latest inventions and introduction of highly sophisticated mass spectrometry equipment sample preparation becomes an extremely important bottleneck of biomedical analysis. We have a goal of giving the reader several successful examples of sample preparation, development and optimization, leading to the success in analytical steps and proper conclusions made at the end of the day. This book is structured as a compilation of contributed chapters ranging from protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.

Author : Cheng Guo,Gerard Bolbach,Lucrece Matheron,Kezhi Jiang,Lei Yue,Lin Wang
Publisher : Frontiers Media SA
Page : 120 pages
File Size : 48,8 Mb
Release : 2022-07-13
Category : Science
ISBN : 9782889764365

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Methodological Advances of MALDI Mass Spectrometry-Based Techniques in Organic and Biomedical Analysis by Cheng Guo,Gerard Bolbach,Lucrece Matheron,Kezhi Jiang,Lei Yue,Lin Wang Pdf

Author : Fernando Cobo
Publisher : Academic Press
Page : 298 pages
File Size : 44,6 Mb
Release : 2018-08-03
Category : Science
ISBN : 9780128144527

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The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology by Fernando Cobo Pdf

The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology presents the state-of the-art for MALDI-TOF mass spectrometry. It is a key reference defining how MALDI-TOF mass spectrometry is used in clinical settings as a diagnostic tool of microbial identification and characterization that is based on the detection of a mass of molecules. The book provides updated applications of MALDI-TOF techniques in clinical microbiology, presenting the latest information available on a technology that is now used for rapid microbial identification at relatively low cost, thus offering an alternative to conventional laboratory diagnosis and proteomic identification systems. Although the main use of the technology has, until now, been identification or typing of bacteria from a positive culture, applications in the field of virology, mycology, microbacteriology and resistances are opening up new opportunities. Presents updated applications of MALDI-TOF techniques in clinical microbiology Describes the use of mass spectrometry in the lab, the principles of the technology, preparation of samples, device calibration and maintenance, treatment of microorganisms, and quality control Presents key information for researchers, including possible uses of the technology, differences between devices, how to interpret results, and future applications Covers the topic in a systematic and comprehensive manner that is useful to both clinicians and researchers

Author : Michael Kinter,Caroline S. Kinter
Publisher : Springer Science & Business Media
Page : 65 pages
File Size : 52,8 Mb
Release : 2013-09-17
Category : Medical
ISBN : 9781461486664

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Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics by Michael Kinter,Caroline S. Kinter Pdf

A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins. ​