Agrobacterium Tumefaciens

Author : Eugene W. Nester,Milton Paul Gordon,Allen Kerr
Publisher : American Phytopathological Society
Page : 344 pages
File Size : 48,8 Mb
Release : 2005
Category : Science
ISBN : STANFORD:36105114577088

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Agrobacterium Tumefaciens by Eugene W. Nester,Milton Paul Gordon,Allen Kerr Pdf

This anthology traces the fascinating progress from plant pathology to biotechnology through 38 scientific papers on Agrobacterium, published over the past century. Included are the seminal scientific papers on the biology and application of Agrobacterium with introductory commentaries mostly by those involved in the original work. The commentaries give background to the papers and explain the problems faced and the techniques used, providing insight into the way fundamental research progresses.Agrobacterium tumefaciens has played a major role in the astounding advances that have been made over the past several decades in the areas of plant genetics, plant molecular biology, and plant genetic engineering. The papers included in this book were integral to the current understanding of the interaction of Agrobacterium with its hosts, its development into a major player in the genetic engineering of plants, and the biological control of crown gall.Agrobacterium tumefaciens: From Plant Pathology to Biotechnology is divided into five sections. The first section begins with 1904 when Erwin F. Smith began detailed work on crown gall and considered it to be a plant pathological problem. It explores many of the biological discoveries made over the past century, including the pivotal moment when Armin C. Braun discovered that crown gall was a plant cancer. Other papers cover the beginnings of T-DNA research and the development of vectors to improve the process of transferring T-DNA from bacterium to plant cell. The second section delves further into vector systems and genetic coding for disease and insect resistance, exploring the evolution of genetic engineering in crops.The final three sections deal with themes developed from crown gall studies, including ?quorum sensing? or population density, the DNA sequencing of one strain of A. tumefaciens, and the first genetically engineered organism, strain K1026, released for commercial use.According to Editor, Eugene Nester, ?This book should serve as a testimony to the 100 years of research on this remarkable organism, as well as to an international group of investigators who helped reveal secrets of this natural genetic engineer.? Students, professors, plant pathologists, microbiologists, or anyone interested in research and/or the history of plant pathology and biotechnology, will find this collection of papers an intriguing read.From the Preface: ??the journey is not over. As the commentary by Paul Hooykaas indicates, it looks as though T-DNA will insert into any cell, be it plant, fungal or even mammalian. Is there a possibility of using Agro?bacterium in gene therapy? Will Agrobacterium prove to be as useful a tool in fungal genetics as it has been in plant genetics? Its potential is mind-boggling.?

Author : Tzvi Tzfira,Vitaly Citovsky
Publisher : Springer Science & Business Media
Page : 768 pages
File Size : 48,5 Mb
Release : 2007-12-25
Category : Science
ISBN : 9780387722900

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Agrobacterium: From Biology to Biotechnology by Tzvi Tzfira,Vitaly Citovsky Pdf

Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.

Author : Kan Wang
Publisher : Springer Science & Business Media
Page : 473 pages
File Size : 55,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597451314

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Agrobacterium Protocols by Kan Wang Pdf

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Author : Kan Wang
Publisher : Springer Science & Business Media
Page : 474 pages
File Size : 40,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597451307

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Agrobacterium Protocols by Kan Wang Pdf

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Author : Phetole Mangena
Publisher : CRC Press
Page : 244 pages
File Size : 54,8 Mb
Release : 2022-09-01
Category : Science
ISBN : 9781000638158

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Plant Transformation via Agrobacterium Tumefaciens by Phetole Mangena Pdf

Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes. This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment. Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.

Author : Shain-Dow Kung,Shang-Fa Yang
Publisher : World Scientific
Page : 384 pages
File Size : 49,7 Mb
Release : 1998-02-17
Category : Science
ISBN : 9789814504492

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Discoveries in Plant Biology by Shain-Dow Kung,Shang-Fa Yang Pdf

As scientific progress hinges on the continual discovery and extension of previous discoveries, this series, Discoveries in Plant Biology, is specially compiled to provide an atlas of the landmark discoveries in the broad span of plant biology. The collection of chapters, written by renowned plant biologists, describe how classic discoveries were made and how they have served as the foundation for subsequent discoveries. We hope that this will facilitate our readers' quest to advance their knowledge based on the advancements made previously by others. The 21 discoveries described in this First Volume all form the foundations of modern plant biology. The contributors, many of whom are themselves the researchers who made the discoveries, bring readers back in time to retrace the steps of the discoveries. Following the creative thoughts of the scientists in deciphering the natural laws, readers may appreciate how each field was developed from a simple subject to an advanced multidisciplinary field. Contents:Abscisic Acid: Discoveries and Exploration of Properties (F T Addicott)History of the Discovery of Ethylene as a Plant Growth Substance (M E Saltveit et al.)The Discovery of Transposable Elements (N Fedoroff)Discovery of T-DNA Agrobacterium Tumefaciens (M P Gordon)The Discovery of Fraction 1 Protein (Rubisco) (S G Wildman)C4 Photosynthesis: Discovery, Resolution Recognition, and Significance (M D Hatch & C R Slack)The Path of Carbon in Photosynthesis: 1942 – 1955 (A A Benson)Discoveries in Biological Nitrogen Fixation (R H Burris)The Discovery of Biological Clocks (F B Salisbury)and other papers Readership: Students and researchers in botany, biochemistry, genetics and plant physiology. keywords:Botany;Plant Biology “This excellent book should be present in all central libraries and in those of plant biology institutions. The book is recommended to advanced students and researchers.” Journal of Plant Physiology

Author : K. Lindsey
Publisher : Springer Science & Business Media
Page : 138 pages
File Size : 52,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789400901810

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Plant Tissue Culture Manual by K. Lindsey Pdf

This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering.

Author : Stanton B. Gelvin
Publisher : Springer
Page : 509 pages
File Size : 42,9 Mb
Release : 2018-11-28
Category : Medical
ISBN : 9783030032579

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Agrobacterium Biology by Stanton B. Gelvin Pdf

This volume reviews various facets of Agrobacterium biology, from modern aspects of taxonomy and bacterial ecology to pathogenesis, bacterial cell biology, plant and fungal transformation, natural transgenics, and biotechnology. Agrobacterium-mediated transformation is the most extensively utilized platform for generating transgenic plants, but modern biotechnology applications derive from more than 40 years of intensive basic scientific research. Many of the biological principles established by this research have served as models for other bacteria, including human and animal pathogens. Written by leading experts and highlighting recent advances, this volume serves both as an introduction to Agrobacterium biology for students as well as a more comprehensive text for research scientists.

Author : Hau-Hsuan Hwang,Stanton B. Gelvin
Publisher : Frontiers Media SA
Page : 167 pages
File Size : 43,5 Mb
Release : 2015-06-26
Category : Botany
ISBN : 9782889195749

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Agrobacterium biology and its application to transgenic plant production by Hau-Hsuan Hwang,Stanton B. Gelvin Pdf

The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.

Author : Ingo Potrykus,German Spangenberg
Publisher : Springer Science & Business Media
Page : 370 pages
File Size : 51,8 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783642792472

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Gene Transfer to Plants by Ingo Potrykus,German Spangenberg Pdf

Author : Günter Kahl,Josef S. Schell
Publisher : Academic Press
Page : 644 pages
File Size : 51,9 Mb
Release : 2014-06-28
Category : Nature
ISBN : 9781483281957

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Molecular Biology of Plant Tumors by Günter Kahl,Josef S. Schell Pdf

Molecular Biology of Plant Tumors provides an opportunity to learn in detail about the latest insights into the mechanism of transformation of plant cells by Agrobacterium tumefaciens. The study of the molecular mechanism responsible for the crown gall phenomenon (induced by Agrobacterium tumefaciens) illustrates the point that the fundamental study of the cause(s) and mechanism(s) of abnormal growth might be one of the most efficient ways to understand cellular differentiation and the molecular basis of gene expression. The book is organized into three parts that contain research on abnormal plant growth, crown gall tumors, and potential vectors for genetic engineering in agriculture. The genetic structure responsible for the neoplasmic transformation of plant cells in crown galls is a bacterial plasmid (called Ti for tumor-inducing). Research described in this volume demonstrates that these Ti plasmids were designed by evolution as natural gene vectors with which some bacteria can introduce active genes into plants. These transferred genes are maintained by integration in the plant genome and their expression is directly or indirectly responsible for the tumorous growth pattern.

Author : A.D. Arencibia
Publisher : Elsevier
Page : 283 pages
File Size : 52,8 Mb
Release : 2000-02-14
Category : Business & Economics
ISBN : 9780080539058

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Plant Genetic Engineering by A.D. Arencibia Pdf

Plant biotechnology offers important opportunities for agriculture, horticulture, and the pharmaceutical and food industry by generating transgenic varieties with altered properties. This is likely to change farming practice and reduce the potential negative impact of plant production on the environment. This volume shows the worldwide advances and potential benefits of plant genetic engineering focusing on the third millennium. The authors discuss the production of transgenic plants resistant to biotic and abiotic stress, the improvement of plant qualities, the use of transgenic plants as bioreactors, and the use of plant genomics for genetic improvement and gene cloning. Unique to this book is the integrative point of view taken between plant genetic engineering and socioeconomic and environmental issues. Considerations of regulatory processes to release genetically modified plants, as well as the public acceptance of the transgenic plants are also discussed.This book will be welcomed by biotechnologists, researchers and students alike working in the biological sciences. It should also prove useful to everyone dedicated to the study of the socioeconomic and environmental impact of the new technologies, while providing recent scientific information on the progress and perspectives of the production of genetically modified plants. The work is dedicated to Professor Marc van Montagu.

Author : Kan Wang
Publisher : Humana
Page : 484 pages
File Size : 53,5 Mb
Release : 2006-06-01
Category : Science
ISBN : 1588295362

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Agrobacterium Protocols by Kan Wang Pdf

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Author : Paul Anthony Spencer
Publisher : Unknown
Page : 96 pages
File Size : 52,6 Mb
Release : 1988
Category : Agrobacterium tumefaciens
ISBN : OCLC:20248084

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Signal Compound Specificity in Agrobacterium Tumefaciens by Paul Anthony Spencer Pdf

Author : Yasunari Ogihara,Shigeo Takumi,Hirokazu Handa
Publisher : Springer
Page : 445 pages
File Size : 53,9 Mb
Release : 2015-09-15
Category : Science
ISBN : 9784431556756

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Advances in Wheat Genetics: From Genome to Field by Yasunari Ogihara,Shigeo Takumi,Hirokazu Handa Pdf

This proceedings is a collection of 46 selected papers that were presented at the 12th International Wheat Genetics Symposium (IWGS). Since the launch of the wheat genome sequencing project in 2005, the arrival of draft genome sequences has marked a new era in wheat genetics and genomics, catalyzing rapid advancement in the field. This book provides a comprehensive review of the forefront of wheat research, across various important topics such as germplasm and genetic diversity, cytogenetics and allopolyploid evolution, genome sequencing, structural and functional genomics, gene function and molecular biology, biotic stress, abiotic stress, grain quality, and classical and molecular breeding. Following an introduction, 9 parts of the book are dedicated to each of these topics. A final, 11th part entitled “Toward Sustainable Wheat Production” contains 7 excellent papers that were presented in the 12th IWGS Special Session supported by the OECD. With rapid population growth and radical climate changes, the world faces a global food crisis and is in need of another Green Revolution to boost yields of wheat and other widely grown staple crops. Although this book focuses on wheat, many of the newly developed techniques and results presented here can be applied to other plant species with large and complex genomes. As such, this volume is highly recommended for all students and researchers in wheat sciences and related plant sciences and for those who are interested in stable food production and food security.