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Agrobacterium: From Biology to Biotechnology by Tzvi Tzfira,Vitaly Citovsky Pdf
Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.
This volume reviews various facets of Agrobacterium biology, from modern aspects of taxonomy and bacterial ecology to pathogenesis, bacterial cell biology, plant and fungal transformation, natural transgenics, and biotechnology. Agrobacterium-mediated transformation is the most extensively utilized platform for generating transgenic plants, but modern biotechnology applications derive from more than 40 years of intensive basic scientific research. Many of the biological principles established by this research have served as models for other bacteria, including human and animal pathogens. Written by leading experts and highlighting recent advances, this volume serves both as an introduction to Agrobacterium biology for students as well as a more comprehensive text for research scientists.
Agrobacterium biology and its application to transgenic plant production by Hau-Hsuan Hwang,Stanton B. Gelvin Pdf
The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.
Plant Transformation Technologies by Charles Neal Stewart,Alisher Touraev,Vitaly Citovsky,Tzvi Tzfira Pdf
Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.
Agrobacterium Tumefaciens by Eugene W. Nester,Milton Paul Gordon,Allen Kerr Pdf
This anthology traces the fascinating progress from plant pathology to biotechnology through 38 scientific papers on Agrobacterium, published over the past century. Included are the seminal scientific papers on the biology and application of Agrobacterium with introductory commentaries mostly by those involved in the original work. The commentaries give background to the papers and explain the problems faced and the techniques used, providing insight into the way fundamental research progresses.Agrobacterium tumefaciens has played a major role in the astounding advances that have been made over the past several decades in the areas of plant genetics, plant molecular biology, and plant genetic engineering. The papers included in this book were integral to the current understanding of the interaction of Agrobacterium with its hosts, its development into a major player in the genetic engineering of plants, and the biological control of crown gall.Agrobacterium tumefaciens: From Plant Pathology to Biotechnology is divided into five sections. The first section begins with 1904 when Erwin F. Smith began detailed work on crown gall and considered it to be a plant pathological problem. It explores many of the biological discoveries made over the past century, including the pivotal moment when Armin C. Braun discovered that crown gall was a plant cancer. Other papers cover the beginnings of T-DNA research and the development of vectors to improve the process of transferring T-DNA from bacterium to plant cell. The second section delves further into vector systems and genetic coding for disease and insect resistance, exploring the evolution of genetic engineering in crops.The final three sections deal with themes developed from crown gall studies, including ?quorum sensing? or population density, the DNA sequencing of one strain of A. tumefaciens, and the first genetically engineered organism, strain K1026, released for commercial use.According to Editor, Eugene Nester, ?This book should serve as a testimony to the 100 years of research on this remarkable organism, as well as to an international group of investigators who helped reveal secrets of this natural genetic engineer.? Students, professors, plant pathologists, microbiologists, or anyone interested in research and/or the history of plant pathology and biotechnology, will find this collection of papers an intriguing read.From the Preface: ??the journey is not over. As the commentary by Paul Hooykaas indicates, it looks as though T-DNA will insert into any cell, be it plant, fungal or even mammalian. Is there a possibility of using Agro?bacterium in gene therapy? Will Agrobacterium prove to be as useful a tool in fungal genetics as it has been in plant genetics? Its potential is mind-boggling.?
Genetic transformation of plants has revolutionized both basic and applied plant research. Plant molecular biology and physiology benefit from this power fool, as well as biotechnology. This book is a review of some of the most significant achievements that plant transformation has brought to the fields of Agrobacterium biology, crop improvement and, flower, fruit and tree amelioration. Also, it examines their impact on molecular farming, phytoremediation and RNAi tools.
The Rhizobiaceae by Herman P. Spaink,Adam Kondorosi,Paul J.J. Hooykaas Pdf
Comprises 26 contributions that provide an overview of the present molecular biological knowledge about the Rhizobiaceae, a family of soil bacteria that interact with and affect the development of plants. In addition to covering the various bacteria and their activities, the book also discusses the scientific principles that have been discovered as a result of study in the discipline. Topics include outer membrane proteins, alternative membrane lipids, the production of exopolysaccharides, opines and opine-like molecules involved in plant-Rhizobiaceae interactions, conjugal plasmids and their transfer, the use of Agrobacterium for plant genetic engineering, functions of rhizobial nodulation genes, and the agronomic aspects of legume symbiotic nitrogen fixation. Intended for professionals in chemistry, biochemistry, genetics, and biology, or as a textbook for a second or third year graduate course in microbiology or plant-microbe interactions. Annotation copyrighted by Book News, Inc., Portland, OR
Genetic Transformation of Plants by John Flex Jackson,Hans F. Linskens Pdf
Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not me an that all chapters apriori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen re cent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis.
Molecular Biology of Plant Tumors by Günter Kahl,Josef S. Schell Pdf
Molecular Biology of Plant Tumors provides an opportunity to learn in detail about the latest insights into the mechanism of transformation of plant cells by Agrobacterium tumefaciens. The study of the molecular mechanism responsible for the crown gall phenomenon (induced by Agrobacterium tumefaciens) illustrates the point that the fundamental study of the cause(s) and mechanism(s) of abnormal growth might be one of the most efficient ways to understand cellular differentiation and the molecular basis of gene expression. The book is organized into three parts that contain research on abnormal plant growth, crown gall tumors, and potential vectors for genetic engineering in agriculture. The genetic structure responsible for the neoplasmic transformation of plant cells in crown galls is a bacterial plasmid (called Ti for tumor-inducing). Research described in this volume demonstrates that these Ti plasmids were designed by evolution as natural gene vectors with which some bacteria can introduce active genes into plants. These transferred genes are maintained by integration in the plant genome and their expression is directly or indirectly responsible for the tumorous growth pattern.
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.
Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.
Discoveries in Plant Biology by Shain-Dow Kung,Shang-Fa Yang Pdf
As scientific progress hinges on the continual discovery and extension of previous discoveries, this series, Discoveries in Plant Biology, is specially compiled to provide an atlas of the landmark discoveries in the broad span of plant biology. The collection of chapters, written by renowned plant biologists, describe how classic discoveries were made and how they have served as the foundation for subsequent discoveries. We hope that this will facilitate our readers' quest to advance their knowledge based on the advancements made previously by others. The 21 discoveries described in this First Volume all form the foundations of modern plant biology. The contributors, many of whom are themselves the researchers who made the discoveries, bring readers back in time to retrace the steps of the discoveries. Following the creative thoughts of the scientists in deciphering the natural laws, readers may appreciate how each field was developed from a simple subject to an advanced multidisciplinary field. Contents:Abscisic Acid: Discoveries and Exploration of Properties (F T Addicott)History of the Discovery of Ethylene as a Plant Growth Substance (M E Saltveit et al.)The Discovery of Transposable Elements (N Fedoroff)Discovery of T-DNA Agrobacterium Tumefaciens (M P Gordon)The Discovery of Fraction 1 Protein (Rubisco) (S G Wildman)C4 Photosynthesis: Discovery, Resolution Recognition, and Significance (M D Hatch & C R Slack)The Path of Carbon in Photosynthesis: 1942 – 1955 (A A Benson)Discoveries in Biological Nitrogen Fixation (R H Burris)The Discovery of Biological Clocks (F B Salisbury)and other papers Readership: Students and researchers in botany, biochemistry, genetics and plant physiology. keywords:Botany;Plant Biology “This excellent book should be present in all central libraries and in those of plant biology institutions. The book is recommended to advanced students and researchers.” Journal of Plant Physiology
Biology of the Rhizobiaceae by Kenneth L. Giles,Alan G. Atherly Pdf
Biology of the Rhizobiaceae covers the genetics, molecular biology, agricultural, and morphological aspects of the rhizobia. The book discusses the taxonomy and identification of the Rhizobiaceae; the biology of Agrobacterium tumefaciens and the specific events in the disease cycle of crown gall; and the agricultural control of Agrobacterium tumefaciens. The text also describes the growth potential of crown gall tumors and crown gall teratoma; plasmid studies in crown gall tumorigenesis; and the biology and microbiology of Agrobacterium rhizogenes. The recognition in rhizobium-legume symbioses; the rhizobium bacteroid state; and the exchange of metabolites and energy between legume and rhizobium are also considered. The book further tackles the mutants of rhizobium that are altered in legume interaction and nitrogen fixation; as well as the significance and application of Rhizobium in agriculture. Botanists, agriculturists, geneticists, molecular biologists, microbiologists, plant pathologists, and agronomists will find the book invaluable.
