Artificial Self Assembling Systems For Gene Delivery

Author : Philip L. Felgner
Publisher : Unknown
Page : 216 pages
File Size : 43,7 Mb
Release : 1996
Category : Language Arts & Disciplines
ISBN : WISC:89058941451

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Artificial Self-assembling Systems for Gene Delivery by Philip L. Felgner Pdf

Discusses recent advances in artificial self-assembling systems, including retrotransposon vectors, hairpin ribozymes, triple-helix-forming oligonucleotides, liposome-mediated transfection, and photonic nanostructures. Addresses developing efficient synthetic vectors such as modular self-assembling systems mimicking important features of viral vectors. Presents new developments in synthetic self-assembling gene delivery systems, including innovations in nonviral systems, targeting nucleic acids, ligand-polylysine mediated transfer, dendrimer-mediated transfection, cationic liposomes, and polylysine DNA complexes.

Author : Alexander V. Kabanov,Philip L. Felgner,Leonard W. Seymour
Publisher : Wiley-Blackwell
Page : 492 pages
File Size : 46,7 Mb
Release : 1998-05-29
Category : Medical
ISBN : UOM:39015042032857

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Self-Assembling Complexes for Gene Delivery by Alexander V. Kabanov,Philip L. Felgner,Leonard W. Seymour Pdf

Recent years have witnessed an explosion of activity in the field of gene therapy. Following advances in our understanding of the molecular basis of disease, hopes are high that the tremendous potential market for drugs employing antisense nucleotides and genes will one day be fulfilled. However, many obstacles remain, not least problems in the technology of gene delivery systems. Much of today's research focuses on non-viral approaches to gene delivery. Of particular importance are supramolecular complexes formed between DNA and various natural and synthetic polymers and lipids, otherwise known as 'self-assembling complexes for gene delivery'. In view of this fact, the editors of this volume have assembled an international team of contributors to present up-to-date reviews of the major chemical, biological and clinical aspects of such gene delivery vectors. Reflecting the diversity of research in this field, this book comprehensively covers: * the principles of self-assembly * natural mechanisms for gene delivery to cells * cationic lipids and liposomes * polyelectrolyte DNA complexes * systemic biodistribution of drug delivery systems * targeting of conjugates for gene delivery * new approaches to gene delivery * clinical evaluation Self-assembling Complexes for Gene Delivery is an essential reference for all professionals with an interest in gene and antisense therapy or novel drug delivery systems, including medicinal and pharmaceutical chemists, clinicians, human geneticists, molecular biologists and pharmacologists.

Author : Dan Luo,W. Mark Saltzman
Publisher : Springer Science & Business Media
Page : 160 pages
File Size : 51,5 Mb
Release : 2003-09-30
Category : Science
ISBN : 0306477017

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Synthetic DNA Delivery Systems by Dan Luo,W. Mark Saltzman Pdf

DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function. Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest. Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.

Author : Ernst Jucker
Publisher : Birkhäuser
Page : 326 pages
File Size : 48,7 Mb
Release : 2013-03-12
Category : Medical
ISBN : 9783034888455

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Progress in Drug Research by Ernst Jucker Pdf

Author : Danilo D. Lasic
Publisher : CRC Press
Page : 326 pages
File Size : 41,9 Mb
Release : 2019-07-23
Category : Science
ISBN : 9780429605727

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Liposomes in Gene Delivery by Danilo D. Lasic Pdf

Many specialists are not familiar with both drug delivery and the molecular biology of DNA vectors. Liposomes in Gene Delivery covers both-molecular biologists will gain a basic knowledge of lipids, liposomes, and other gene delivery vehicles; lipid and drug delivery scientists will better understand DNA, molecular biology, and DNA manipulation. Topics include an introduction to nucleic acids, a theoretical description of DNA, recombinant technology, lipids and liposomes, stability and interaction properties of lipids and liposomes, complexation of lipids and liposomes with DNA plasmids, gene expression of genosomes in various models, structure-activity relationships, and transfection models. This is an excellent introductory text for graduate students, scientists, and researchers in molecular and cell biology, genetics, biochemistry, physical chemistry, colloid science, pharmacology, molecular science, and medicine.

Author : Keith L. March
Publisher : Springer Science & Business Media
Page : 516 pages
File Size : 52,8 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461562771

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Gene Transfer in the Cardiovascular System by Keith L. March Pdf

The goal of gene transfer is protein expression. a process brought about by the insertion of a gene coding for a foreign protein into target cells resulting in the synthesis of the foreign protein For gene therapy, a tmnsferred therapeutic gene must be expressed at a level beneficial for the patient. This chapter provides an introductory overview of the rapidly evolving field of non-viral approaches for gene delivery to rnarnrnalian cells. Although currently there are fewer ongoing clinical trials using non-viral approaches than those using viral based systems, the number of non-viral trials is increasing. The long range goal of some research groups is the development of a genetically engineered artificial virus targeted to specific cells in the human body. An arurual conference, organized by Cambridge Healthtech Institute entitled "Artificial Self-Assembling Systems for Gene Transfer", brings together researchers interested in this field [1]. Assembly of an artificial virus is very complex; other research groups aim to develop simpler delivery systems consisting of a plasmid combined with delivery agents. Viral-based systems are very successful for gene delivery, but despite their successes, viral-based systems have some geneml limitations and system-specific limitations. When employing a viml-based system, the following limitations should be considered: • size limitation of the inserted gene due to packaging constraints (e. g. adenovirus, retrovirus) . • potential tumorigenesis (e. g. retrovirus) • potential for insertional mutagenesis (greater than plasmid based systems) • potential imrnunogenicity (e. g.

Author : Roger Bertolotti,Syed Hasan Parvez,T. Nagatsu
Publisher : VSP
Page : 576 pages
File Size : 51,7 Mb
Release : 2000-09-28
Category : Science
ISBN : 9067643289

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Progress in Gene Therapy by Roger Bertolotti,Syed Hasan Parvez,T. Nagatsu Pdf

Gene Therapy is expected to revolutionize the practice of medicine at the turn of the third Millennium. Therapeutic/prophylactic benefits should arise from both gene transfer and gene repair/inactivation protocols devised for patient's somatic cells. Gene expression cassettes, designed for the production of therapeutic proteins and non-coding RNA, are thus experimented together with emerging gene repair/inactivation techniques on a variety of inherited, acquired and infectious/parasitic diseases, including complex neuro-degenerative processes. This book presents a collection of chapters on the main aspects of Gene Therapy, some of which have already been treated in the past, and updates and further develops the current survey. It is aimed at understanding why Human Gene Therapy is likely to be a medical breakthrough, although definitive clinical success still needs time to accomplish. The contributions focus on both technical/biomedical concepts and on experimental/clinical data that appear to confer potential universality to Gene Therapy. This book does not claim to provide an exhaustive review of the pathologies, which are currently approached with Gene Therapy. The presentation of current and emerging approaches, together with problems and tentative issues aims to serve as a booster for the development of new applications in every field of medicine and will, therefore, be of value and interest to researchers in this exciting field.

Author : Gregory Gregoriadis,Brenda McCormack
Publisher : Springer Science & Business Media
Page : 201 pages
File Size : 45,8 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461564058

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Targeting of Drugs 5 by Gregory Gregoriadis,Brenda McCormack Pdf

Proceedings of a NATO ASI held in Cape Sounion, Greece, June 24-July 5, 1995

Author : Anya M. Hillery,Andrew W. Lloyd,James Swarbrick
Publisher : CRC Press
Page : 448 pages
File Size : 55,7 Mb
Release : 2003-09-02
Category : Medical
ISBN : 9780203302767

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Drug Delivery and Targeting by Anya M. Hillery,Andrew W. Lloyd,James Swarbrick Pdf

The advances in biotechnology and molecular biology over recent years have resulted in a large number of novel molecules with the potential to revolutionize the treatment and prevention of disease. However, such potential is severely compromised by significant obstacles to delivery of these drugs in vivo. These obstacles are often so great that effective drug delivery and targeting is now recognized as the key to effective development of many therapeutics. Advanced drug delivery and targeting can offer significant advantages to conventional drugs, such as increased efficiency, convenience, and the potential for line extensions and market expansion. An accessible and easy-to-read textbook, Drug Delivery and Targeting for Pharmacists and Pharmaceutical Scientists is the first book to provide a comprehensive introduction to the principles of advanced drug delivery and targeting, their current applications and potential future developments, including: *Methods to optimize therapeutic efficacy, and the related commercial implications *Difficulties with drug absorption, unwanted distribution and premature inactivation / elimination *Attempts to minimize toxicity or alter immunogenicity *Methods to achieve rate-controlled drug release and effective drug targeting *Novel and established routes of delivery *Use of new generation technologies such as biosensors, microchips, stimuli-sensitive hydrogels and plasmid-based gene therapy This volume is indispensable for pharmaceutical students, scientists and researchers.

Author : Anya Hillery,Kinam Park
Publisher : CRC Press
Page : 633 pages
File Size : 49,7 Mb
Release : 2016-09-15
Category : Medical
ISBN : 9781482217728

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Drug Delivery by Anya Hillery,Kinam Park Pdf

This book provides a comprehensive introduction to advanced drug delivery and targeting, covering their principles, current applications, and potential future developments. This edition has been updated to reflect significant trends and cutting-edge advances that have occurred since the first edition was published. All the original chapters have been retained, but the material therein has been updated. Eight new chapters have been added that deal with entirely new technologies and approaches. Features: Offers a comprehensive introduction to the fundamental concepts and underlying scientific principles of drug delivery and targeting Presents an in-depth analysis of the opportunities and obstacles afforded by the application of nanotechnologies for drug delivery and targeting Includes a revised and expanded section on the major epithelial routes of drug delivery currently under investigation Describes the most recent, emerging, and innovative technologies of drug delivery Provides real-life examples of the clinical translation of drug delivery technologies through the use of case studies Discusses the pertinent regulatory hurdles and safety issues of drug delivery and targeting systems—crucial considerations in order to achieve licensing approval for these new technologies

Author : Brian H. Robinson
Publisher : IOS Press
Page : 484 pages
File Size : 54,5 Mb
Release : 2003
Category : Computers
ISBN : 1586033824

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Self-assembly by Brian H. Robinson Pdf

The book contains six sections. The first section covers general articles; then there is a section concentrating on novel systems and applications. This is followed by one that deals with a range of applications of polymers, surfactants and liquid crystals. This is followed by a section on advances in fundamental understanding. Then there is one on biological systems, and finally there is a section on micelle and vesicle systems, with particular emphasis on dynamic aspects. The contributors, including Physicists, Chemists, Biologists and Chemical Engineers, variously chose to write review-type articles, summaries of their own recent work in the field and its relevance in the general concept of self-assembly, specific short papers related to their particular presentation, or their own thoughts concerning the future development of their particular interest area. All these aspects are addressed in the book. The book covers research at the forefront of the subject, and it is expected to be a very useful addition to the literature in this important field.

Author : Mien-Chie Hung,Leaf Huang,Ernst Wagner
Publisher : Elsevier
Page : 456 pages
File Size : 44,5 Mb
Release : 1999-07-01
Category : Science
ISBN : 0080479774

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Nonviral Vectors for Gene Therapy by Mien-Chie Hung,Leaf Huang,Ernst Wagner Pdf

Gene transfer within humans has been an obstacle until about 10 years ago. At that time, it was found that viral vectors were effective carriers of "healthy genes" into patients' cells. The problem, however, was that viral vectors proved unnecessarily harmful to humans: subjects experienced inflamatory activity and negative immunological responses to the genes. Viral vectors were also unable to meet the needs of the pharmaceutical community: they were not reproducible in large-scale proportions in cost-effective ways. Thus, research was undertaken to find a safer way to transfer genes to patients without jeopardizing the safety of the patient. And so non-viral vectors were discovered. This volume presents the various non-viral vectors currently under development. Although not methodologically oriented, it will provide the necessary details behind the development of the vectors. This information will prove useful to both researchers and clinicians. Key Features * Presents state-of-the art developments of nonviral vectors as tools for modern molecular medicine * Covers all types of nonviral vectors, from molecular structure to therapeutic application Provides a comprehensive review of synthetic vectors * Includes contributions from major investigators and leading experts in the field

Author : Susanna Wu-Pong
Publisher : Springer Science & Business Media
Page : 352 pages
File Size : 46,7 Mb
Release : 1999-04-01
Category : Medical
ISBN : 9781592597055

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Biopharmaceutical Drug Design and Development by Susanna Wu-Pong Pdf

New discoveries in biology are occurring at an incredible rate, and with these discoveries arise nearly unimaginable opportunities in every area of human existence. Imagine the excitement surround ing the "penicillin project" and the subsequent rapid development of anti-infective agents that took place in the 1940s and 1950s. Fast for ward to the world today and our ability to treat life-threatening infec tions. This is but one small piece in the present kaleidoscope of new therapeutic agents. In fact, the world of science, biology, and medi cine is changing so quickly that it is difficult for scientists and medi cal practitioners to stay abreast of their fields and confidently anticipate that their education and training will sustain them over a three- to four-decade career without considerable continuing educa tion and training. For the pharmaceutical scientist responsible for the discovery and development of therapeutic agents based on advances in biotechnology, it is imperative to quickly come up to speed and stay at the forefront of developments, which is no easy task for those not specifically trained in this area. Biopharmaceutical Drug Design and Development, edited by Susanna Wu-Pong and Yongyut Rojanasakul, cuts a potentially wide swath in terms of its intended audience. It clearly is a primer for those not trained in the area, or for those who wish to be brought into the mainstream of drug discovery and development in the world of bio technology.

Author : G. Walsh,B. Murphy
Publisher : Springer Science & Business Media
Page : 515 pages
File Size : 54,6 Mb
Release : 2013-03-09
Category : Science
ISBN : 9789401709262

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Biopharmaceuticals, an Industrial Perspective by G. Walsh,B. Murphy Pdf

This book provides a unique and up-to-date insight into the biopharmaceutical industry. Largely written by industrial authors, its scope is multidisciplinary, rendering it an ideal reference source for students undertaking advanced undergraduate or postgraduate courses in biotechnology, pharmaceutical science, biochemistry, or medicine.

Author : Ivan Coluzza
Publisher : Springer
Page : 132 pages
File Size : 55,9 Mb
Release : 2018-03-23
Category : Medical
ISBN : 9783319715780

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Design of Self-Assembling Materials by Ivan Coluzza Pdf

This book provides in-depth insights into assembling dynamics of proteins, DNA and other nanoparticles. The applications of basic knowledge in the development of artificial self-assembling systems will be discussed and state of the art methodology in the field will be presented.This interdisciplinary work brings together aspects of different fields of expertise such as Biology, Physics and Material Sciences and is intended for researchers, professors and graduate students interested in the design of self-assembling materials.