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Author : Ellen Covey,Matt Carter Publisher : Oxford University Press, USA Page : 241 pages File Size : 42,8 Mb Release : 2015 Category : Medical ISBN : 9780199939800
Basic Electrophysiological Methods by Ellen Covey,Matt Carter Pdf
A broad, concise, and no-nonsense guide to contemporary electrophysiological techniques, covering intracellular and extracellular recording through recording of population activity, neuropharmacology, dye imaging, voltammetry, and optogenetics.
Introduction to Electrophysiological Methods and Instrumentation by Franklin Bretschneider,Jan R. de Weille Pdf
Introduction to Electrophysiological Methods and Instrumentation, Second Edition covers all topics of interest to electrophysiologists, neuroscientists and neurophysiologists, from the reliable penetration of cells and the behavior and function of the equipment, to the mathematical tools available for analyzing data. It discusses the pros and cons of techniques and methods used in electrophysiology and how to avoid pitfalls. Although the basics of electrophysiological techniques remain the principal purpose of this second edition, it now integrates several current developments, including, amongst others, automated recording for high throughput screening and multimodal recordings to correlate electrical activity with other physiological parameters collected by optical means. This book provides the electrophysiologist with the tools needed to understand his or her equipment and how to acquire and analyze low-voltage biological signals. Introduces possibilities and solutions, along with the problems, pitfalls, and artefacts of equipment and electrodes Discusses the particulars of recording from brain tissue slices, oocytes and planar bilayers Describes optical methods pertinent to electrophysiological practice Presents the fundamentals of signal processing of analogue signals, spike trains and single channel recordings, along with procedures for signal recording and processing Includes appendices on electrical safety and foundations of useful mathematical tools
Electrophysiology by Jürgen Rettinger,Silvia Schwarz,Wolfgang Schwarz Pdf
This updated and revised textbook presents a broad overview on topics concerning cellular electrophysiology – ranging from bioelectric phenomena recognized as far back as ancient Egypt to popular topics on the dangers of electrosmog. Without sacrificing scientific precision, this clear and concise work presents on the one hand the different methods and modern applications, on the other hand the biophysical fundamentals of ion-channel and carrier proteins. Numerous and carefully selected illustrations and diagrams supplement the text. Each chapter is extended by a detailed table of Take-Home Messages. Questions at the end of each chapter allow readers to test their understanding. Each section also includes references to relevant original literature for further reading. The book offers a valuable resource for students of biology, chemistry and physics with a special interest in biophysics.
Electrophysiological Methods in Biological Research by Jan Bures,Mojmir Petran,Jozef Zachar Pdf
Electrophysiological Methods in Biological Research, Third Revised Edition describes the principles and applications of significant electrophysiological methods as regards to transistorisation of electrophysiological apparatus and to the mathematical analysis of electrophysiological data. The book explains the aspects of physics and electronics that are important in electrophysiology, such as the basic principles of semiconductor function, electronic simulators, electrodes, and the processing of electrophysiological data. The text also cites several examples that measure the resulting membrane potential if one electrode is inside the cell while the other is in contact with the cell's surface. Other experiments show the electrophysiological techniques and the fundamentals of electrical activity in the peripheral excitable structures, and its association with physiological functions. In considering the problems of nerve and muscle physiology, the investigator should know the technique of recording the electrical signs of a nerve impulse. These signs, or action potentials, indicate the presence of a nerve impulse. The text also discusses the effects of barbiturates or ether anesthesia in EEG activity, as well as its dissociation after physostigmine and atropine have been administered. The book can prove useful for pharmacologists, microchemists, cellular biologists, and research workers and technologists dealing with neural mechanisms.
Author : Robert P. Vertes,Robert W. Stackman, Jr. Publisher : Humana Press Page : 0 pages File Size : 52,6 Mb Release : 2010-11-19 Category : Medical ISBN : 1603272011
Electrophysiological Recording Techniques by Robert P. Vertes,Robert W. Stackman, Jr. Pdf
Applying neurophysiological methods to the study of brain-behavior relationships proved to be a major advance in the early days of neuroscience research. Considerable technological progress has been made very recently, and the impact on modern neuroscience will be invaluable. In Electrophysiological Recording Techniques, experts in the field present a current view of the widespread application of electrophysiological methods to the study of the brain and to the problem of brain-behavior relationships. The book has been organized to display the range of modern neurophysiological methods ranging from the recordings of single neurons and neuronal ensembles to recordings of field potentials within discrete brain regions and across multiple brain areas. Many of the chapters also address the major challenge of applying the appropriate methods to analyze and interpret neurophysiological recording data. As a volume in the popular Neuromethods series, the chapters provide authoritative reviews of many commonly used approaches in the field today in both the basic research level and in clinical settings. Practical and up-to-date, Electrophysiological Recording Techniques serves as a key reference volume for researchers working in this ever-changing and vital field.
Electrophysiology by Jürgen Rettinger,Silvia Schwarz,Wolfgang Schwarz Pdf
This textbook presents a broad overview of topics concerning cellular electrophysiology – covering topics ranging from bioelectric phenomena recognized as far back as ancient Egypt to popular topics on the dangers of electrosmog. Without sacrificing scientific precision, this clear and concise work presents on the one hand the different methods and applications, on the other hand the biophysical fundamentals of ion-channel and carrier proteins. Numerous and carefully selected illustrations and diagrams supplement the text, while questions at the end of each chapter allow readers to test their understanding. Each section also includes references to relevant original literature for further reading. The book offers a valuable resource for students of biology, chemistry and physics with a special interest in biophysics.
Guide to Research Techniques in Neuroscience by Matt Carter,Rachel Essner,Nitsan Goldstein,Manasi Iyer Pdf
Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step
Author : Daniel C. Sigg,Paul A. Iaizzo,Yong-Fu Xiao,Bin He Publisher : Springer Science & Business Media Page : 492 pages File Size : 54,6 Mb Release : 2010-09-11 Category : Medical ISBN : 9781441966582
Cardiac Electrophysiology Methods and Models by Daniel C. Sigg,Paul A. Iaizzo,Yong-Fu Xiao,Bin He Pdf
Cardiovascular disease is the major cause of mortality and morbidity in the Western Hemisphere. While significant progress has been made in treating a major sub-category of cardiac disease, arrhythmias, significant unmet needs remain. In particular, every day, thousands of patients die because of arrhythmias in the US alone, and atrial fibrillation is the most common arrhythmia affecting millions of patients in the US alone at a given time. Therefore, there is a public need to continue to develop new and better therapies for arrhythmias. Accordingly, an ever increasing number of biomedical, pharmaceutical, and medical personnel is interested in studying various aspects of arrhythmias at a basic, translational, and applied level, both in industry (ie Biotech, Pharmaceutical and device), and in academia. Not only has our overall understanding of molecular bases of disease dramatically increased, but so has the number of available and emerging molecular, pharmacological or device treatment based therapies. This practical, state-of-the art handbook will summarize and review key research methods and protocols, their advantages and pitfalls, with a focus on practical implementation, and collaborative cross-functional research. The volume will include visual and easy-to-use graphics, bulleted summaries, boxed summary paragraphs, links to reference websites, equipment manufacturers where appropriate, photographs of typical experimental setups and so forth, to keep this book very focused on practical methods and implementation, and yet, provide enough theory that the principles are clearly understood and can be easily applied.
John D. Durrant,Cynthia G. Fowler,John A. Ferraro,Suzanne C. Purdy
Author : John D. Durrant,Cynthia G. Fowler,John A. Ferraro,Suzanne C. Purdy Publisher : Plural Publishing Page : 505 pages File Size : 45,6 Mb Release : 2022-01-07 Category : Medical ISBN : 9781635501797
Basic Concepts of Clinical Electrophysiology in Audiology by John D. Durrant,Cynthia G. Fowler,John A. Ferraro,Suzanne C. Purdy Pdf
Basic Concepts of Clinical Electrophysiology in Audiology is a revolutionary textbook, combining the research and expertise of both distinguished experts and up-and-coming voices in the field. By taking a multidisciplinary approach to the subject, the editors of this graduate-level text break down all aspects of electrophysiology to make it accessible to audiology students. In addition to defining the basics of the tools of the trade and their routine uses, the authors also provide ample presentations of new approaches currently undergoing continuing research and development. The goal of this textbook is to give developing audiologists a broad and solid basis of understanding of the methods in common or promising practice. Throughout the text, individual chapters are divided into “episodes,” each examining a facet of the overarching chapter’s topic. With different experts handling each episode, readers are exposed to outstanding professionals in the field. This text singularly stitches together the chapters and their episodes to build from foundational concepts to more complex issues that clinicians are likely to face on their road to full clinical competency. As collections of episodes, the writers and editors thus endeavor to present a series of stories that build throughout the book, in turn allowing readers to build a broader interest in the subject. Key Features * Heads Up sections in each chapter introduce more advanced content to expose readers to what lies beyond the basic level and further enhance the main chapter content and “entertainment value” * Take home messages at the end of each chapter serve to focus the reader’s attention, encourage review, and discourage superficial learning by “just reading the abstract” * More than 450 innovative illustrations use combinations of panels, insets, and/or gray tone to facilitate reader understanding, optimize portrayal of data, and unify concepts across chapters * Numerous case studies and references to practical clinical issues and results are included throughout the book * Keywords are highlighted in-text to improve both attention and retention of critical terms and ease of returning to review them
Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns
Author : Alan A. Boulton,Glen B. Baker,Case H. Vanderwolf Publisher : Springer Science & Business Media Page : 416 pages File Size : 55,5 Mb Release : 1990 Category : Medical ISBN : UCAL:B5036525
Neurophysiological Techniques by Alan A. Boulton,Glen B. Baker,Case H. Vanderwolf Pdf
The latest information and methodologies for studying the activity of living nervous tissue, with special emphasis on electrophysiological techniques and their applications, ranging from ion channels to human behavior.
Patch Clamp Electrophysiology by Mark Dallas,Damian Bell Pdf
This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.
Practical Electrophysiological Methods by Helmut Kettenmann,Rosemarie Grantyn Pdf
Designed to enable newcomers to electrophysiology to choose appropriate preparations and techniques for in vitro studies in vertebrate neurobiology. Emphasizes techniques involving the glass micropipette and other recently developed methods, supplementing easy-to-follow instructions with illustrative schemes, examples of original records, photomicrographs, key word listings, comprehensive reference tables and a list of suppliers and key equipment and reagents.
Electrophysiological Analysis of Synaptic Transmission by Nicholas Graziane,Yan Dong Pdf
Electrophysiological methods are used at nearly all levels of biological research today. From basic science investigation to bringing new drugs to market, electrophysiology is an indispensable research technique that provides essential insights into cellular and molecular functions. This work is intended to be a practical guide for understanding and using contemporary electrophysiological manipulations and assays in brain research. New researchers attempting to learn electrophysiological techniques are confronted with a lack of adequate guiding resources. Currently, most electrophysiology-based research knowledge is passed on by experienced individuals to others within the same laboratory. But, this master-apprentice scenario has many inherent difficulties, as it is both a time intensive process and also leads to incomplete knowledge transfer, or even superstitious and maladaptive practices. Few external guiding resources exist for a budding researcher who wishes to gain the skills necessary to conduct responsible and competent electrophysiology research. Dr. Yan Dong, an expert in utilizing electrophysiological techniques for brain research with fifteen years of experience, and Peter Neumann hope this work can act as a concise and practical aid to remedy the current situation. This book will cover both basic methods and advanced techniques clearly and in depth, as well as the essential background and theory necessary to understand the methods. Additionally, this book also covers conventional and contemporary patch-clamp methods, experimental design, data collection and analysis, common pitfalls to avoid, important nuances, technical concerns, practical applications, and advice for teaching new researchers. Together, this represents a contemporary best-practices guide for collecting and interpreting electrophysiological data for brain research.
Cognitive Electrophysiology of Attention by George R. Mangun Pdf
Cognitive Electrophysiology of Attention explores the fundamental mechanisms of attention and related cognitive functions from cognitive neuroscience perspectives. Attention is an essential cognitive ability that enables humans to process and act upon relevant information while ignoring distracting information, and the capacity to focus attention is at the core of mental functioning. Understanding the neural bases of human attention remains a key challenge for neuroscientists and psychologists, and is essential for translational efforts to treat attentional deficits in a variety of neurological and psychiatric disorders. Cognitive electrophysiology is at the center of a multidisciplinary approach that involves the efforts of psychologists, neuroscientists, neuropsychologists, psychiatrists, and neurologists to identify basic brain mechanisms and develop translational approaches to improve mental health. This edited volume is authored by leading investigators in the field and discusses methods focused on electrophysiological recordings in humans, including electroencephalography (EEG) and event-related potential (ERP) methods, and also incorporates evidence from functional magnetic resonance imaging (fMRI). Cognitive Electrophysiology of Attention illuminates specific models about attentional mechanisms in vision, audition, multisensory integration, memory, and semantic processing in humans. Provides an exhaustive overview of attention processes, going from normal functioning to the pathological, and using a combination of methodological tools An important reference for electrophysiology researchers looking at underlying attention processes rather than the methods themselves Enables researchers across a broad range of cognitive-process and methodological specialties to stay current on particular hypotheses, findings, and methods Edited and authored by the worldwide leaders in the field, affording the broadest, most expert coverage available