Cell Culture And Upstream Processing

Author : Michael Butler
Publisher : Garland Science
Page : 233 pages
File Size : 49,9 Mb
Release : 2007-08-07
Category : Science
ISBN : 9781134148295

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Cell Culture and Upstream Processing by Michael Butler Pdf

Upstream processing refers to the production of proteins by cells genetically engineered to contain the human gene which will express the protein of interest. The demand for large quantities of specific proteins is increasing the pressure to boost cell culture productivity, and optimizing bioreactor output has become a primary concern for most pharmaceutical companies. Each chapter in Cell Culture and Upstream Processing is taken from presentations at the highly acclaimed IBC conferences as well as meetings of the European Society for Animal Cell Technology (ESACT) and Protein Expression in Animal Cells (PEACe) and describes how to improve yield and optimize the cell culture production process for biopharmaceuticals, by focusing on safety, quality, economics and operability and productivity issues. Cell Culture and Upstream Processing will appeal to a wide scientific audience, both professional practitioners of animal cell technology as well as students of biochemical engineering or biotechnology in graduate or high level undergraduate courses at university.

Author : Gyun Min Lee,Helene Faustrup Kildegaard
Publisher : John Wiley & Sons
Page : 436 pages
File Size : 41,9 Mb
Release : 2020-01-13
Category : Science
ISBN : 9783527343348

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Cell Culture Engineering by Gyun Min Lee,Helene Faustrup Kildegaard Pdf

Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.

Author : Mohamed Al-Rubeai
Publisher : Springer
Page : 766 pages
File Size : 46,5 Mb
Release : 2014-11-28
Category : Medical
ISBN : 9783319103204

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Animal Cell Culture by Mohamed Al-Rubeai Pdf

​Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

Author : Hansjörg Hauser,Roland Wagner
Publisher : Walter de Gruyter GmbH & Co KG
Page : 718 pages
File Size : 54,5 Mb
Release : 2014-11-10
Category : Science
ISBN : 9783110278965

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Animal Cell Biotechnology by Hansjörg Hauser,Roland Wagner Pdf

This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.

Author : Amine Kamen,Laura Cervera
Publisher : CRC Press
Page : 267 pages
File Size : 54,5 Mb
Release : 2022-09-06
Category : Medical
ISBN : 9781000643053

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Bioprocessing of Viral Vaccines by Amine Kamen,Laura Cervera Pdf

This book focuses on cell culture-produced viral vaccines to meet the needs of the rapidly expanding research and development in academia and industry in the field. This book introduces the basic principles of vaccination and the manufacturing of viral vaccines. Bioprocessing of Viral Vaccines, will provide an overview of the advanced strategies needed to respond to the challenges of new and established viral infection diseases. The first few chapters cover the basics of virology and immunology as essential concepts to understand the function and design of viral vaccines. The core of the content is dedicated to process development, including upstream processing and cell culture of viral vaccines, downstream processing, and extensive analytical technologies specific to viral vaccines. Advanced process analytical technologies (PAT) and Quality by Design (QbD) concepts are also introduced in the context of vaccine manufacturing. The case studies included cover inactivated, attenuated vaccines exemplified by influenza vaccines, sub-unit vaccines exemplified by Virus Like Particles (VLPs: HPV vaccines) and sub-unit vaccines (Flublock), vectored vaccines: adenoviruses and Vesicular stomatitis Virus (VSV) vectored vaccines, genomic vaccines (DNA and mRNA) vaccines as developed for COVID-19 response in particular and a review of COVID-19 vaccines approved or in advanced clinical trials. This book is aimed at graduate engineers and professionals in the fields of vaccinology, bioprocessing, and biomanufacturing of viral vaccines.

Author : Owen P. Ward
Publisher : Buckingham [England] : Open University Press
Page : 216 pages
File Size : 47,5 Mb
Release : 1991
Category : Science
ISBN : UOM:39015022058070

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Bioprocessing by Owen P. Ward Pdf

Author : Moritz Wolf,Jean-Marc Bielser,Massimo Morbidelli
Publisher : Cambridge University Press
Page : 219 pages
File Size : 45,8 Mb
Release : 2020-08-06
Category : Technology & Engineering
ISBN : 9781108846400

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Perfusion Cell Culture Processes for Biopharmaceuticals by Moritz Wolf,Jean-Marc Bielser,Massimo Morbidelli Pdf

Master the design and operation of perfusion cell cultures with this authoritative reference. Discover the current state-of-the-art in the design and operation of continuous bioreactors, with emphasis on mammalian cell cultures for producing therapeutic proteins. Topics include the current market for recombinant therapeutic proteins, current industry challenges and the potential contribution of continuous manufacturing. Provides coverage of every step of process development and reactor operation, including small scale screening to lab-scale and scale-up to manufacturing scale. Illustrated through real-life case studies, this is a perfect resource for groups active in the cell culture field, as well as graduate students in areas such as chemical engineering, biotechnology, chemistry and biology, and to those in the pharmaceutical industry, particularly biopharma, biotechnology and food or agro industry.

Author : Lubiniecki
Publisher : Routledge
Page : 656 pages
File Size : 53,8 Mb
Release : 2018-05-02
Category : Medical
ISBN : 9781351435710

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Large-Scale Mammalian Cell Culture Technology by Lubiniecki Pdf

An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator

Author : Lubiniecki
Publisher : Routledge
Page : 412 pages
File Size : 48,9 Mb
Release : 2018-05-02
Category : Medical
ISBN : 9781351435703

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Large-Scale Mammalian Cell Culture Technology by Lubiniecki Pdf

An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator

Author : Bob Kiss,Uwe Gottschalk,Michael Pohlscheidt
Publisher : Springer
Page : 473 pages
File Size : 53,8 Mb
Release : 2018-12-06
Category : Science
ISBN : 9783319971100

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New Bioprocessing Strategies: Development and Manufacturing of Recombinant Antibodies and Proteins by Bob Kiss,Uwe Gottschalk,Michael Pohlscheidt Pdf

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Author : Michael Butler
Publisher : Garland Science
Page : 250 pages
File Size : 47,9 Mb
Release : 2007-06-30
Category : Science
ISBN : 9780203967232

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Cell Culture and Upstream Processing by Michael Butler Pdf

Upstream processing refers to the production of proteins by cells genetically engineered to contain the human gene which will express the protein of interest. The demand for large quantities of specific proteins is increasing the pressure to boost cell culture productivity, and optimizing bioreactor output has become a primary concern for most pharmaceutical companies. Each chapter in Cell Culture and Upstream Processing is taken from presentations at the highly acclaimed IBC conferences as well as meetings of the European Society for Animal Cell Technology (ESACT) and Protein Expression in Animal Cells (PEACe) and describes how to improve yield and optimize the cell culture production process for biopharmaceuticals, by focusing on safety, quality, economics and operability and productivity issues. Cell Culture and Upstream Processing will appeal to a wide scientific audience, both professional practitioners of animal cell technology as well as students of biochemical engineering or biotechnology in graduate or high level undergraduate courses at university.

Author : Goutam Saha,Alok Barua,Satyabroto Sinha
Publisher : CRC Press
Page : 126 pages
File Size : 50,8 Mb
Release : 2017-12-01
Category : Medical
ISBN : 9781498736022

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Bioreactors by Goutam Saha,Alok Barua,Satyabroto Sinha Pdf

Bioreactors: Animal Cell Culture Control for Bioprocess Engineering presents the design, fabrication, and control of a new type of bioreactor meant especially for animal cell line culture. The new bioreactor, called the "see-saw bioreactor," is ideal for the growth of cells with a sensitive membrane. The see-saw bioreactor derives its name from its principle of operation in which liquid columns in either limb of the reactor alternately go up and down. The working volume of the reactor is small, to within 15 L. However, it can easily be scaled up for large production in volume of cell mass in the drug and pharmaceutical industries. The authors describe the principle of operation of the see-saw bioreactor and how to automatically control the bioprocess. They discuss different control strategies as well as the thorough experimental research they conducted on this prototype bioreactor in which they applied a time delay control for yield maximization. To give you a complete understanding of the design and development of the see-saw bioreactor, the authors cover the mathematical model they use to describe the kinetics of fermentation, the genetic algorithms used for deriving the optimal time trajectories of the bioprocess variables, and the corresponding control inputs for maximizing the product yield. One chapter is devoted to the application of time delay control. Following a description of the bioreactor’s working setup in the laboratory, the authors sum up their investigation and define the future scope of work in terms of design, control, and software sensors.

Author : Wei-Shu Hu
Publisher : Springer
Page : 0 pages
File Size : 50,8 Mb
Release : 2010-11-18
Category : Science
ISBN : 3642070515

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Cell Culture Engineering by Wei-Shu Hu Pdf

Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade.

Author : Sadettin Ozturk,Wei-Shou Hu
Publisher : CRC Press
Page : 788 pages
File Size : 55,7 Mb
Release : 2005-08-30
Category : Medical
ISBN : 9780849351068

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Cell Culture Technology for Pharmaceutical and Cell-Based Therapies by Sadettin Ozturk,Wei-Shou Hu Pdf

Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology devel

Author : Terence Cartwright
Publisher : Cambridge University Press
Page : 193 pages
File Size : 44,7 Mb
Release : 1994-08-26
Category : Medical
ISBN : 9780521412582

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Animal Cells as Bioreactors by Terence Cartwright Pdf

Because of their complexity, the new generation of genetically engineered protein drugs can only be made by biotechnological methods, using cultures of animal cells. This book covers all aspects of the technologies needed to turn animal cells into an acceptable and cost-effective tool for drug production. This includes modifying them genetically so that they produce the right product in high yield, getting them to grow reproducibly on an industrial scale, and extracting the required product from them. It also covers biological safety issues, and the verification of the chemical and biological nature of the protein drug produced. The work covers developments in all of these areas and how they all need to be integrated for the design of an effective biotechnological production process. It therefore provides a comprehensive guide to this area of biotechnology.