Cell Imaging Techniques

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Cell Imaging Techniques

Douglas J. Taatjes,Brooke T. Mossman
Cell Imaging Techniques Book PDF

Author : Douglas J. Taatjes,Brooke T. Mossman
Publisher : Springer Science & Business Media
Page : 505 pages
File Size : 42,6 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592599936

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Cell Imaging Techniques by Douglas J. Taatjes,Brooke T. Mossman Pdf

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Cellular Imaging Techniques for Neuroscience and Beyond

Floris G. Wouterlood
Cellular Imaging Techniques for Neuroscience and Beyond Book PDF

Author : Floris G. Wouterlood
Publisher : Academic Press
Page : 298 pages
File Size : 43,7 Mb
Release : 2012-08-08
Category : Medical
ISBN : 9780123858726

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Cellular Imaging Techniques for Neuroscience and Beyond by Floris G. Wouterlood Pdf

In the biomedical sciences, the confocal laser scanning microscope (CLSM) has become the instrument of choice for producing high-resolution images and 3D reconstruction, breaking the barriers of conventional optical microscopy. Wouterlood (anatomy, VU U. Medical Center, Amsterdam, the Netherlands) introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin. The text includes color and b&w images, and referral to an online CLSM simulator. Academic Press is an imprint of Elsevier. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com).

Cellular Imaging

Eric Hanssen
Cellular Imaging Book PDF

Author : Eric Hanssen
Publisher : Springer
Page : 323 pages
File Size : 44,5 Mb
Release : 2017-12-30
Category : Science
ISBN : 9783319689975

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Cellular Imaging by Eric Hanssen Pdf

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

Live Cell Imaging

Sung-Bae Kim
Live Cell Imaging Book PDF

Author : Sung-Bae Kim
Publisher : Humana
Page : 447 pages
File Size : 50,5 Mb
Release : 2022-06-12
Category : Science
ISBN : 1071612603

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Live Cell Imaging by Sung-Bae Kim Pdf

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

Live Cell Imaging

Robert D. Goldman,David L. Spector
Live Cell Imaging Book PDF

Author : Robert D. Goldman,David L. Spector
Publisher : CSHL Press
Page : 658 pages
File Size : 54,8 Mb
Release : 2005
Category : Science
ISBN : 0879696834

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Live Cell Imaging by Robert D. Goldman,David L. Spector Pdf

Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.

Methods in Cellular Imaging

Ammasi Periasamy
Methods in Cellular Imaging Book PDF

Author : Ammasi Periasamy
Publisher : Springer
Page : 473 pages
File Size : 55,6 Mb
Release : 2013-05-27
Category : Science
ISBN : 9781461475132

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Methods in Cellular Imaging by Ammasi Periasamy Pdf

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

Advanced Imaging and Bio Techniques for Convergence Science

Jun Ki Kim,Jeong Kon Kim,Chan-Gi Pack
Advanced Imaging and Bio Techniques for Convergence Science Book PDF

Author : Jun Ki Kim,Jeong Kon Kim,Chan-Gi Pack
Publisher : Springer Nature
Page : 560 pages
File Size : 46,7 Mb
Release : 2021-04-08
Category : Medical
ISBN : 9789813360648

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Advanced Imaging and Bio Techniques for Convergence Science by Jun Ki Kim,Jeong Kon Kim,Chan-Gi Pack Pdf

This book is a wide-ranging guide to advanced imaging techniques and related methods with important applications in translational research or convergence science as progress is made toward a new era in integrative healthcare. Conventional and advanced microscopic imaging techniques, including both non-fluorescent (i.e., label-free) and fluorescent methods, have to date provided researchers with specific and quantitative information about molecules, cells, and tissues. Now, however, the different imaging techniques can be correlated with each other and multimodal methods developed to simultaneously obtain diverse and complementary information. In addition, the latest advanced imaging techniques can be integrated with non-imaging techniques such as mass spectroscopic methods, genome editing, organic/inorganic probe synthesis, nanomedicine, and drug discovery. The book will be of high value for researchers in the biological and biomedical sciences or convergence science who need to use these multidisciplinary and integrated techniques or are involved in developing new analytical methods focused on convergence science.

Cellular Imaging Techniques for Neuroscience and Beyond

Floris G. Wouterlood
Cellular Imaging Techniques for Neuroscience and Beyond Book PDF

Author : Floris G. Wouterlood
Publisher : Academic Press
Page : 298 pages
File Size : 53,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9780123858733

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Cellular Imaging Techniques for Neuroscience and Beyond by Floris G. Wouterlood Pdf

The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc. Single and multiphoton confocal microscopy, and 4-pi confocal microscopy Obtaining nanoresolution via photoactivation localization microscopy (PALM) Several procedures that correlate observations in optical fluorescence microscopy and electron microscopy Study of morphology and function via high-resolution fluorescence procedures Additional high-resolution microscopic techniques

Visualizing Chemistry

National Research Council,Division on Earth and Life Studies,Board on Chemical Sciences and Technology,Committee on Revealing Chemistry through Advanced Chemical Imaging
Visualizing Chemistry Book PDF

Author : National Research Council,Division on Earth and Life Studies,Board on Chemical Sciences and Technology,Committee on Revealing Chemistry through Advanced Chemical Imaging
Publisher : National Academies Press
Page : 222 pages
File Size : 40,5 Mb
Release : 2006-06-01
Category : Science
ISBN : 9780309164634

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Visualizing Chemistry by National Research Council,Division on Earth and Life Studies,Board on Chemical Sciences and Technology,Committee on Revealing Chemistry through Advanced Chemical Imaging Pdf

Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.

Cell Imaging Techniques

Douglas J. Taatjes,Brooke T. Mossman
Cell Imaging Techniques Book PDF

Author : Douglas J. Taatjes,Brooke T. Mossman
Publisher : Unknown
Page : 490 pages
File Size : 40,5 Mb
Release : 2005
Category : Biochemistry
ISBN : 128083191X

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Cell Imaging Techniques by Douglas J. Taatjes,Brooke T. Mossman Pdf

Cell imaging methodologies have now become essential research tools for a variety of disciplines that traditionally had not relied on them. In Cell Imaging Techniques: Methods and Protocols, distinguished international researchers describe in detail their state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complementary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. The protocols follow the successful Methods in Molecular Biology"!series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and highly practical, Cell Imaging Techniques: Methods and Protocols provides researchers and clinicians with a richly useful guide to selecting and performing the best imaging method from a bewildering variety of microscopy-based techniques

Fluorescent Materials for Cell Imaging

Fu-Gen Wu
Fluorescent Materials for Cell Imaging Book PDF

Author : Fu-Gen Wu
Publisher : Springer
Page : 247 pages
File Size : 41,8 Mb
Release : 2021-11-10
Category : Medical
ISBN : 9811550646

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Fluorescent Materials for Cell Imaging by Fu-Gen Wu Pdf

This book focuses on the latest fluorescent materials for cell imaging. Cell imaging is a widely used basic technique that helps scientists gain a better understanding of biological functions through studies of cellular structure and dynamics. In the past decades, the development of a variety of new fluorescent materials has significantly extended the applications of cellular imaging techniques. This book presents recently developed fluorescent materials, including semiconductor quantum dots, carbon dots, silicon nanoparticles, metal nanoclusters, upconversion nanoparticles, conjugated polymers/polymer dots, aggregation-induced emission (AIE) probes, and coordination compounds, used for various cellular imaging purposes. It will appeal to cell biologists and other researchers in academia, industry and clinical settings who are interested in the technical development and advanced applications of fluorescence imaging in cells, tissues and organisms to explore the mechanisms of biological functions and diseases.

Cell Imaging Techniques

Douglas J. Taatjes,Jürgen Roth
Cell Imaging Techniques Book PDF

Author : Douglas J. Taatjes,Jürgen Roth
Publisher : Humana Press
Page : 550 pages
File Size : 47,7 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493962469

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Cell Imaging Techniques by Douglas J. Taatjes,Jürgen Roth Pdf

Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.

Imaging from Cells to Animals In Vivo

Margarida Barroso,Xavier Intes
Imaging from Cells to Animals In Vivo Book PDF

Author : Margarida Barroso,Xavier Intes
Publisher : CRC Press
Page : 444 pages
File Size : 47,7 Mb
Release : 2020-12-03
Category : Science
ISBN : 9781351704496

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Imaging from Cells to Animals In Vivo by Margarida Barroso,Xavier Intes Pdf

This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.

Quantitative Imaging in Cell Biology

Anonim
Quantitative Imaging in Cell Biology Book PDF

Author : Anonim
Publisher : Academic Press
Page : 588 pages
File Size : 44,6 Mb
Release : 2014-06-25
Category : Science
ISBN : 9780124202016

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Quantitative Imaging in Cell Biology by Anonim Pdf

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

In Vivo Optical Imaging of Brain Function, Second Edition

Ron D. Frostig
In Vivo Optical Imaging of Brain Function Second Edition Book PDF

Author : Ron D. Frostig
Publisher : CRC Press
Page : 428 pages
File Size : 45,9 Mb
Release : 2009-05-06
Category : Science
ISBN : 9781420076851

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In Vivo Optical Imaging of Brain Function, Second Edition by Ron D. Frostig Pdf

These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques. New in the Second Edition: Voltage-sensitive dyes imaging in awake behaving animals Imaging based on genetically encoded probes Imaging of mitochondrial auto-fluorescence as a tool for cortical mapping Using pH-sensitive dyes for functional mapping Modulated imaging Calcium imaging of neuronal activity using 2-photon microscopy Fourier approach to optical imaging Fully updated chapters from the first edition Leading Authorities Explore the Latest Techniques Updated to reflect continuous development in this emerging research area, this new edition, as with the original, reaches across disciplines to review a variety of non-invasive optical techniques used to study activity in the living brain. Leading authorities from such diverse areas as biophysics, neuroscience, and cognitive science present a host of perspectives that range from a single neuron to large assemblies of millions of neurons, captured at various temporal and spatial resolutions. Introducing techniques that were not available just a few years ago, the authors describe the theory, setup, analytical methods, and examples that highlight the advantages of each particular method.