Crystallization Of Biological Macromolecules

Author : Alexander McPherson
Publisher : Unknown
Page : 608 pages
File Size : 50,6 Mb
Release : 1999
Category : Medical
ISBN : UOM:39015043795395

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Crystallization of Biological Macromolecules by Alexander McPherson Pdf

This extensively illustrated book by Alexander McPherson, a master practitioner, accomplishes several important goals: it presents the underlying physical and chemical principles of crystallization in an approachable way; it provides the reader with a biochemical context in which to understand and pursue successful crystal growth; it instructs the reader in practical aspects of the technologies required; and it lays out effective strategies for success that investigators can readily apply to their own experimental questions. This readable volume has been created for every investigator in biomedicine whose studies may require a shift in focus from gene to protein product, as well as chemists and physicists interested in the functions of biologically active macromolecules.

Author : Arnaud Ducruix,Richard Giegé
Publisher : Unknown
Page : 460 pages
File Size : 52,8 Mb
Release : 1999
Category : Science
ISBN : 9780199636785

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Crystallization of Nucleic Acids and Proteins by Arnaud Ducruix,Richard Giegé Pdf

Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucleic Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucleic Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.

Author : Naomi E Chayen,John R Helliwell,Edward H Snell
Publisher : OUP Oxford
Page : 240 pages
File Size : 50,5 Mb
Release : 2010-03-25
Category : Science
ISBN : 9780191550027

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Macromolecular Crystallization and Crystal Perfection by Naomi E Chayen,John R Helliwell,Edward H Snell Pdf

The crystallization of proteins and nucleic acids and/or their complexes has become more highly automated but is still often a trial and error based approach. In parallel, a number of X-ray diffraction based techniques have been developed which explain the physical reasons limiting the resulting crystallographic data and thus show how that data may be improved. Crystal growth is also pivotal in neutron crystallography, which establishes the hydrogen and hydration aspects. Thus this book is aimed at addressing the science behind obtaining the best and most complete structural data possible for biological macromolecules, so that the detailed structural biology and chemistry of these important molecules emerge. Crystal imperfections such as twinning and lattice disorders, as well as multiple crystal situations, and their possible remedies, are also described. The small crystal frontier in micro-crystal crystallography, crystallites in powders and finally down to the proposed single molecule structure determination of X-ray lasers are covered. Overall this interdisciplinary book will interest crystal growers, X-ray and neutron physicists and the biological crystallographers, including graduate students.

Author : Hartmut Michel
Publisher : CRC Press
Page : 236 pages
File Size : 40,9 Mb
Release : 2018-01-18
Category : Medical
ISBN : 9781351079723

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Crystallization of Membrane Proteins by Hartmut Michel Pdf

The precise knowledge of the structure of biological macromolecules forms the basis of understanding their function and their mechanism of action. It also lays the foundation for rational protein and drug design. The only method to obtain this knowledge is still crystallography. At present, the structures of about 400 proteins are known at or nearly at atomic proteins. However, only two of them are membrane proteins or complexes of the membrane proteins. The reasons for the difference is not the crystals of membrane proteins resists forming special problems when being analysed. The reason is that the membrane proteins resist into forming into well-ordered crystals. The intention of this book is to help to produce well-ordered crystals proteins and to provide guidelines, it is aimed at both biochemists and protein crystallographer‘s.

Author : Jaime Gómez Morales
Publisher : MDPI
Page : 184 pages
File Size : 49,7 Mb
Release : 2019-09-23
Category : Science
ISBN : 9783039214037

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Biological Crystallization by Jaime Gómez Morales Pdf

For at least six hundred million years, life has been a fascinating laboratory of crystallization, referred to as biomineralization. During this huge lapse of time, many organisms from diverse phyla have developed the capability to precipitate various types of minerals, exploring distinctive pathways for building sophisticated structural architectures for different purposes. The Darwinian exploration was performed by trial and error, but the success in terms of complexity and efficiency is evident. Understanding the strategies that those organisms employ for regulating the nucleation, growth, and assembly of nanocrystals to build these sophisticated devices is an intellectual challenge and a source of inspiration in fields as diverse as materials science, nanotechnology, and biomedicine. However, “Biological Crystallization” is a broader topic that includes biomineralization, but also the laboratory crystallization of biological compounds such as macromolecules, carbohydrates, or lipids, and the synthesis and fabrication of biomimetic materials by different routes. This Special Issue collects 15 contributions ranging from biological and biomimetic crystallization of calcium carbonate, calcium phosphate, and silica-carbonate self-assembled materials to the crystallization of biological macromolecules. Special attention has been paid to the fundamental phenomena of crystallization (nucleation and growth), and the applications of the crystals in biomedicine, environment, and materials science.

Author : So Iwata
Publisher : Internat'l University Line
Page : 392 pages
File Size : 43,7 Mb
Release : 2003
Category : Science
ISBN : 0963681796

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Methods and Results in Crystallization of Membrane Proteins by So Iwata Pdf

Author : Sylvie Doublie
Publisher : Springer Science & Business Media
Page : 283 pages
File Size : 53,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597452090

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Macromolecular Crystallography Protocols, Volume 1 by Sylvie Doublie Pdf

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization. The volume concludes with a survey of available crystallographic software.

Author : Jaime Gómez Morales,Giuseppe Falini,Juan Manuel García Ruiz
Publisher : Unknown
Page : 1 pages
File Size : 49,9 Mb
Release : 2019
Category : Electronic books
ISBN : 3039214047

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Biological Crystallization by Jaime Gómez Morales,Giuseppe Falini,Juan Manuel García Ruiz Pdf

For at least six hundred million years, life has been a fascinating laboratory of crystallization, referred to as biomineralization. During this huge lapse of time, many organisms from diverse phyla have developed the capability to precipitate various types of minerals, exploring distinctive pathways for building sophisticated structural architectures for different purposes. The Darwinian exploration was performed by trial and error, but the success in terms of complexity and efficiency is evident. Understanding the strategies that those organisms employ for regulating the nucleation, growth, and assembly of nanocrystals to build these sophisticated devices is an intellectual challenge and a source of inspiration in fields as diverse as materials science, nanotechnology, and biomedicine. However, "Biological Crystallization" is a broader topic that includes biomineralization, but also the laboratory crystallization of biological compounds such as macromolecules, carbohydrates, or lipids, and the synthesis and fabrication of biomimetic materials by different routes. This Special Issue collects 15 contributions ranging from biological and biomimetic crystallization of calcium carbonate, calcium phosphate, and silica-carbonate self-assembled materials to the crystallization of biological macromolecules. Special attention has been paid to the fundamental phenomena of crystallization (nucleation and growth), and the applications of the crystals in biomedicine, environment, and materials science.

Author : Jan Sedzik
Publisher : World Scientific
Page : 365 pages
File Size : 40,8 Mb
Release : 2009
Category : Medical
ISBN : 9789812832665

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Molecules by Jan Sedzik Pdf

The word OC nucleation, OCO derived from OC nuclear family, OCO refers to the concept of the progenitor, or the mother and the father of any family. Only in the last few centuries have physicists OC borrowedOCO the word, and more recently, biologists for Theodor Schwann''s cell theory. Most recently, the term has come into use in atomic theory, spectroscopy, and radioactivity, as well as in the fields of atomic bombs, fission, and fusion. Nucleation as a physicochemical process is followed by two poorly understood phenomena OCo aggregation and crystallization - which underlie disorders like Alzheimer''s and OC mad-cowOCO disease (aggregation of amyloid plaque), cardiovascular diseases (deposition in coronary vessels of cholesterol and lipids), and the appearance of crystals under physiological conditions (gout, silicoses, and liver or kidney stones).Written by leading scientists in the field, including one Nobel Laureate, this book provides a unique perspective between the physical and chemical sciences on the one hand, and the biological and medical sciences on the other, and should be of considerable value to scientists, physicians, students, and the interested lay publi

Author : Michael G. Rossmann,Edward V. Arnold
Publisher : Springer
Page : 128 pages
File Size : 44,7 Mb
Release : 2010-04-15
Category : Science
ISBN : 1402092741

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International Tables for Crystallography,Volume F: by Michael G. Rossmann,Edward V. Arnold Pdf

International Tables for Crystallography are no longer available for purchase from Springer. For further information please contact Wiley Inc. International Tables for Crystallography, Volume F, Crystallography of biological macromolecules is an expert guide to macromolecular crystallography for the modern structural biologist. It was commissioned by the International Union of Crystallography in recognition of the extraordinary contributions that knowledge of macromolecular structure has made, and will make, to the analysis of biological systems, from enzyme catalysis to the workings of a whole cell, and to the growing field of structural genomics. The volume covers all stages of a crystallographic analysis from the preparation of samples using the techniques of molecular biology, through crystallization, diffraction data collection, phase determination, structure validation and structure analysis. Although the book is written for experienced scientists, it is recognized that the reader is more likely to be a biologist interested in structure than a classical crystallographer interested in biology. Thus there are chapters on the fundamentals, history and current perspectives of macromolecular crystallography, as well as the availability of useful programs and databases including the Protein Data Bank. Each chapter is written by an internationally recognized expert.

Author : Terese M. Bergfors
Publisher : Internat'l University Line
Page : 505 pages
File Size : 43,7 Mb
Release : 2009
Category : Medical
ISBN : 9780972077446

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Protein Crystallization by Terese M. Bergfors Pdf

Author : Abel Moreno
Publisher : MDPI
Page : 91 pages
File Size : 45,9 Mb
Release : 2019-01-15
Category : Electrical engineering. Electronics. Nuclear engineering
ISBN : 9783038975199

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Protein Crystallization under the Presence of an Electric Field Special Issue Editor Abel Moreno MDPI • by Abel Moreno Pdf

This book is a printed edition of the Special Issue "Protein Crystallization under the Presence of an Electric Field" that was published in Crystals

Author : Bernhard Rupp
Publisher : Garland Science
Page : 832 pages
File Size : 50,6 Mb
Release : 2009-10-20
Category : Science
ISBN : 9781134064199

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Biomolecular Crystallography by Bernhard Rupp Pdf

Synthesizing over thirty years of advances into a comprehensive textbook, Biomolecular Crystallography describes the fundamentals, practices, and applications of protein crystallography. Illustrated in full-color by the author, the text describes mathematical and physical concepts in accessible and accurate language. Biomolecular Crystallography will be a valuable resource for advanced undergraduate and graduate students and practitioners in structural biology, crystallography, and structural bioinformatics.

Author : Naomi E. Chayen
Publisher : Internat'l University Line
Page : 300 pages
File Size : 55,8 Mb
Release : 2007
Category : Science
ISBN : 097207743X

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Protein Crystallization Strategies for Structural Genomics by Naomi E. Chayen Pdf

Author : Heinz K. Henisch
Publisher : Courier Corporation
Page : 132 pages
File Size : 43,6 Mb
Release : 1996-01-01
Category : Science
ISBN : 0486689158

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Crystal Growth in Gels by Heinz K. Henisch Pdf

First book ever printed on growing crystals in a gel medium provides thorough descriptions of the procedure, its history and future potential. "Concise and readable."—Science. 42 illus. 1970 edition.