Author : V. Barak
Publisher : Unknown
Page : 400 pages
File Size : 48,7 Mb
Release : 2002-12-01
Category : Electronic
ISBN : 0896038009
Cytokine Receptor Protocols
V. Barak
Cytokine Receptor Protocols
Cytokine Receptor Protocols Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Cytokine Receptor Protocols book. This book definitely worth reading, it is an incredibly well-written.
Cytokine Protocols
Marc De Ley
Author : Marc De Ley
Publisher : Humana Press
Page : 241 pages
File Size : 47,7 Mb
Release : 2011-11-18
Category : Science
ISBN : 1617794384
Cytokine Protocols by Marc De Ley Pdf
“Recent studies have discovered new known and characterized cytokines, allowing for advancement in miniaturization of micro-analytical methods as well as the extensive development of bio-informatics and nanotechnology. These advancements have allowed researchers to reduces sample sizes making for more accurate determinations then previously possible. In Cytokine Protocols: Second Edition, expert researchers in the field detail many of the methods which are now commonly used to study cytokines. These methods and techniques for studying cytokines include historical importance and the importance of researchers using bioassay, quantification, and characterization of cytokine related RNAs, posttranscriptional modifications of RNA, either naturally or artificially, and observations at the protein level. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and practical, Cytokine Protocols: Second Edition seeks to aid scientists in furthering the crucially important advancement of cytokine research.”
Tissue-Protective Cytokines
Pietro Ghezzi,Anthony Cerami
Author : Pietro Ghezzi,Anthony Cerami
Publisher : Humana Press
Page : 342 pages
File Size : 55,8 Mb
Release : 2016-05-01
Category : Medical
ISBN : 1493962442
Tissue-Protective Cytokines by Pietro Ghezzi,Anthony Cerami Pdf
Thorough and informative, this Methods in Molecular Biology book presents key models used to characterize the tissue-protective actions of cytokines. Offers lists of materials and reagents, step-by-step laboratory protocols, troubleshooting tips and pitfalls."
Cytokine Protocols
Marc De Ley
Author : Marc De Ley
Publisher : Springer Science & Business Media
Page : 248 pages
File Size : 50,8 Mb
Release : 2008-02-03
Category : Medical
ISBN : 9781592596676
Cytokine Protocols by Marc De Ley Pdf
A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Enjoy a quick and smooth introduction to the key methods used in cytokine research Use readily reproducible techniques that ensure successful experimental results Employ antisense-RNA, RT-PCR of small amounts of mRNA, and in situ hybridization.
Cytokine Bioassays
Ivana Vancurova
Author : Ivana Vancurova
Publisher : Humana
Page : 0 pages
File Size : 46,8 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493948504
Cytokine Bioassays by Ivana Vancurova Pdf
Cytokines are pleiotropic regulatory proteins involved in essentially all biological processes and associated with a wide variety of diseases, including inflammatory disorders as well as many types of cancer and leukemia. Knowledge about the quantitative and qualitative nature of cytokine production is critical in the understanding of normal and pathological processes. The cytokine detection in biological and clinical samples faces many challenges including their low abundance, the need to distinguish between active and latent cytokine forms, and the need to measure multiple cytokines in a single assay. This volume will provide a comprehensive collection of classic and cutting-edge methodologies that are currently used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. The chapters are divided into four main categories. The first group focuses on the immunodetection of released cytokines in tissue culture supernatants, plasma, serum and whole blood samples by immunoassays. These immunoassays measure the total concentrations of released cytokines regardless of their biological activity and include ELISA, flow cytometry, ELISPOT and the antibody-based proximity ligation. The second group will focus on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced cell degranulation assay, cell proliferation and differentiation, cytokine-induced cytokine production and the radioreceptor cytokine assay. The third group focuses on the analysis of intracellular cytokines by flow cytometry, western blotting and fluorescence and confocal microscopy. In addition, this category includes protocols for quantitative analysis of cytokine gene expression by real time RT-PCR and analysis of the cytokine promoter occupancy by chromatin immunoprecipitation. The fourth group focuses on the recently developed multiplex arrays that can measure multiple cytokines in the same sample at the same time. This group includes quantification of multiple cytokines using cytometric bead arrays, ELISPOT assays, proteomics cytokine evaluation, multiplexed proximity ligation assays for high-throughput cytokine analysis and finally, cytokine gene expression analysis by gene arrays. The protocols will be written by experienced basic and clinical researchers with hands-on knowledge of the described protocols. By covering a broad variety of methods used in cytokine detection and analysis, this book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research.
NMDA Receptor Protocols
Min Li
Author : Min Li
Publisher : Springer Science & Business Media
Page : 201 pages
File Size : 42,6 Mb
Release : 2008-02-03
Category : Medical
ISBN : 9781592596836
NMDA Receptor Protocols by Min Li Pdf
Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane signaling receptors. NMDA Receptor Protocols offers today's scientists powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.
Adrenergic Receptor Protocols
Curtis A. Machida
Author : Curtis A. Machida
Publisher : Springer Science & Business Media
Page : 563 pages
File Size : 49,7 Mb
Release : 2008-02-03
Category : Medical
ISBN : 9781592596843
Adrenergic Receptor Protocols by Curtis A. Machida Pdf
Adrenergic receptors are important modulators in the sympathetic c- trol of various metabolic processes in the central and peripheral nervous s- tems. These receptors are localized at multiple sites throughout the central nervous system (CNS) and serve as important regulators of CNS-mediated behavior and neural functions, including mood, memory, neuroendocrine c- trol, and stimulation of autonomic function. Adrenergic Receptor Protocols consists of 35 chapters dealing with va- ous aspects of adrenergic receptor analyses, including the use of genetic, RNA, protein expression, transactivator, second messenger, immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches. This volume details the use of various methods to examine the adrenergic receptor system, using aspects of the genetic flow of information as a guide (DNA? RNA ? transactivator ? protein expression ? second messenger analyses ? cellular analyses ? transgenic whole animal approaches). Adrenergic Receptor Protocols displays step-by-step methods for s- cessful replication of experimental procedures, and would be useful for both experienced investigators and newcomers in the field, including those beg- ning graduate study or undergoing postdoctoral training. The Notes section contained in each chapter provides valuable troubleshooting guides to help develop working protocols for your laboratory. With Adrenergic Receptor Protocols, it has been my intent to develop a comprehensive collection of modern molecular methods for analyzing adrenergic receptors. I would like to thank the many chapter authors for their contributions.
Receptor Binding Techniques
Mary Keen
Author : Mary Keen
Publisher : Springer Science & Business Media
Page : 288 pages
File Size : 46,7 Mb
Release : 1999
Category : Binding Sites
ISBN : 9780896035300
Receptor Binding Techniques by Mary Keen Pdf
This cutting-edge collection of step-by-step experimental protocols demonstrates
Military Strategies for Sustainment of Nutrition and Immune Function in the Field
Institute of Medicine,Committee on Military Nutrition Research
Author : Institute of Medicine,Committee on Military Nutrition Research
Publisher : National Academies Press
Page : 722 pages
File Size : 48,7 Mb
Release : 1999-05-13
Category : Medical
ISBN : 9780309173186
Military Strategies for Sustainment of Nutrition and Immune Function in the Field by Institute of Medicine,Committee on Military Nutrition Research Pdf
Every aspect of immune function and host defense is dependent upon a proper supply and balance of nutrients. Severe malnutrition can cause significant alteration in immune response, but even subclinical deficits may be associated with an impaired immune response, and an increased risk of infection. Infectious diseases have accounted for more off-duty days during major wars than combat wounds or nonbattle injuries. Combined stressors may reduce the normal ability of soldiers to resist pathogens, increase their susceptibility to biological warfare agents, and reduce the effectiveness of vaccines intended to protect them. There is also a concern with the inappropriate use of dietary supplements. This book, one of a series, examines the impact of various types of stressors and the role of specific dietary nutrients in maintaining immune function of military personnel in the field. It reviews the impact of compromised nutrition status on immune function; the interaction of health, exercise, and stress (both physical and psychological) in immune function; and the role of nutritional supplements and newer biotechnology methods reported to enhance immune function. The first part of the book contains the committee's workshop summary and evaluation of ongoing research by Army scientists on immune status in special forces troops, responses to the Army's questions, conclusions, and recommendations. The rest of the book contains papers contributed by workshop speakers, grouped under such broad topics as an introduction to what is known about immune function, the assessment of immune function, the effect of nutrition, and the relation between the many and varied stresses encountered by military personnel and their effect on health.
Chromatin Protocols
Peter B. Becker
Author : Peter B. Becker
Publisher : Springer Science & Business Media
Page : 525 pages
File Size : 52,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596812
Chromatin Protocols by Peter B. Becker Pdf
More than 40 years after the discovery of the nucleosome as the fun- mental unit of chromatin, the multifaceted problem of how variations in ch- matin structure affect the activity of the eukaryotic genome has not been solved. However, during the past few years research on chromatin structure and fu- tion has gained considerable momentum, and impressive progress has been made at the level of concept development as well as filling in crucial detail. The structure of the nucleosome has been visualized at unprecedented reso- tion. Powerful multisubunit enzymes have been identified that alter histone/ DNA interactions in ways that expose regulatory sequences to factors initi- ing and regulating such nuclear processes as transcription. Though the imp- tance of posttranslational modifications of histones, notably their acetylation, has long been known, the finding that a number of bona fide regulators increase transcription by acetylating nucleosomes has lent new support to the old idea that the process of gene regulation is intimately related to the nature of the chromatin environment. A wealth of nonhistone proteins contribute to a continuum of structures with distinct biochemical properties and varying degrees of DNA condensation. Perhaps the most important conclusion from a large number of studies is a fresh appreciation of the dynamic nature of chromatin structure, the built-in flexibility providing the basis for regulation.
NLR Proteins
Pablo Pelegrín
Author : Pablo Pelegrín
Publisher : Springer Nature
Page : 301 pages
File Size : 50,6 Mb
Release : 2024-07-04
Category : Electronic
ISBN : 9781071633502
NLR Proteins by Pablo Pelegrín Pdf
Confocal Microscopy
Stephen W. Paddock
Author : Stephen W. Paddock
Publisher : Springer Science & Business Media
Page : 446 pages
File Size : 44,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597222
Confocal Microscopy by Stephen W. Paddock Pdf
In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.
Flavoprotein Protocols
Steven K. Chapman,Graeme A. Reid
Author : Steven K. Chapman,Graeme A. Reid
Publisher : Springer Science & Business Media
Page : 257 pages
File Size : 55,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592592661
Flavoprotein Protocols by Steven K. Chapman,Graeme A. Reid Pdf
As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.
PCR in Bioanalysis
Stephen J Meltzer
Author : Stephen J Meltzer
Publisher : Springer Science & Business Media
Page : 286 pages
File Size : 52,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592595754
PCR in Bioanalysis by Stephen J Meltzer Pdf
PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.
Cytokines and Pain
L.R. Watkins,S.F. Maier
Author : L.R. Watkins,S.F. Maier
Publisher : Springer
Page : 248 pages
File Size : 55,8 Mb
Release : 2013-03-08
Category : Medical
ISBN : 9783034887496
Cytokines and Pain by L.R. Watkins,S.F. Maier Pdf
Within the past few years, it has become recognized that the immune system communicates to the brain. Substances released from activated immune cells (cytokines) stimulate peripheral nerves, thereby signaling the brain and spinal cord that infection/inflammation has occurred. Additionally, peripheral infection/inflammation leads to de novo synthesis and release of cytokines within the brain and spinal cord. Thus, cytokines effect neural activation both peripherally and centrally. Through this communication pathway, cytokines such as interleukin-1, interleukin-6 and tumor necrosis factor markedly alter brain function, physiology and behavior. One important but underrecognized aspect of this communication is the dramatic impact that immune activation has on pain modulation. The purpose of this book is to examine, for the first time, immune-to-brain communication from the viewpoint of its effect on pain processing. It is aimed both at the basic scientist and health care providers, in order to clarify the major role that substances released by immune cells play in pain modulation. This book contains chapters contributed by all of the major laboratories focused on understanding how cytokines modulate pain. These chapters provide a unique vantage point from which to examine this question, as the summarized work ranges from evolutionary approaches across diverse species, to the basics of the immune response, to the effect of cytokines on peripheral and central nervous system sites, to therapeutic potential in humans.