Flavoprotein Protocols

Author : Steven K. Chapman,Graeme A. Reid
Publisher : Springer Science & Business Media
Page : 257 pages
File Size : 52,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592592661

Get Book

Flavoprotein Protocols by Steven K. Chapman,Graeme A. Reid Pdf

As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.

Author : Stefan Weber,Erik Schleicher
Publisher : Humana
Page : 0 pages
File Size : 55,8 Mb
Release : 2016-09-03
Category : Science
ISBN : 1493941704

Get Book

Flavins and Flavoproteins by Stefan Weber,Erik Schleicher Pdf

In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins. These include review style methods and protocols to exemplify the variety, the power and the success of modern techniques and methods in application to flavoproteins. Part I of this Volume covers general properties, syntheses and applications of free flavins as well as its analogs and flavoproteins. Part II covers characterizations of flavins and flavoproteins using modern experimental techniques as well as theoretical methods. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Flavins and Flavoproteins: Methods and Protocols aids scientists in continuing to tackle the countless questions that need to be answered to more fully comprehend the vast diversity and specificity of flavin-governed biological processes.

Author : Susan Miller,Russ Hille,Bruce Palfey
Publisher : Lulu.com
Page : 677 pages
File Size : 50,8 Mb
Release : 2013-03-01
Category : Science
ISBN : 9781300786405

Get Book

FLAVINS and FLAVOPROTEINS 2011 by Susan Miller,Russ Hille,Bruce Palfey Pdf

This book contains articles based on oral and poster presentations at the 17th International Symposium on Flavins and Flavoproteins, which was held July 24-29, 2011 at the University of California Berkeley in the USA. These triennial conferences highlight the latest advances in the field and the conference proceedings book serves both as documentation of the event and as a reference.

Author : Peter B. Becker
Publisher : Springer Science & Business Media
Page : 525 pages
File Size : 45,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596812

Get Book

Chromatin Protocols by Peter B. Becker Pdf

More than 40 years after the discovery of the nucleosome as the fun- mental unit of chromatin, the multifaceted problem of how variations in ch- matin structure affect the activity of the eukaryotic genome has not been solved. However, during the past few years research on chromatin structure and fu- tion has gained considerable momentum, and impressive progress has been made at the level of concept development as well as filling in crucial detail. The structure of the nucleosome has been visualized at unprecedented reso- tion. Powerful multisubunit enzymes have been identified that alter histone/ DNA interactions in ways that expose regulatory sequences to factors initi- ing and regulating such nuclear processes as transcription. Though the imp- tance of posttranslational modifications of histones, notably their acetylation, has long been known, the finding that a number of bona fide regulators increase transcription by acetylating nucleosomes has lent new support to the old idea that the process of gene regulation is intimately related to the nature of the chromatin environment. A wealth of nonhistone proteins contribute to a continuum of structures with distinct biochemical properties and varying degrees of DNA condensation. Perhaps the most important conclusion from a large number of studies is a fresh appreciation of the dynamic nature of chromatin structure, the built-in flexibility providing the basis for regulation.

Author : Christine Schneider
Publisher : Springer Science & Business Media
Page : 212 pages
File Size : 40,6 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590612

Get Book

Chaperonin Protocols by Christine Schneider Pdf

Author : Elias A Lianos
Publisher : Springer Science & Business Media
Page : 298 pages
File Size : 53,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592592630

Get Book

Eicosanoid Protocols by Elias A Lianos Pdf

In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids. Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.

Author : Kelly P. Kearse
Publisher : Springer Science & Business Media
Page : 357 pages
File Size : 52,7 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592596829

Get Book

T Cell Protocols by Kelly P. Kearse Pdf

The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology. Invariably, in putting together a book like this it is difficult to decide which methods to include and which to leave out. To this end methods were selected from a variety of disciplines, including cellular immunology, b- chemistry, and molecular biology, to try to provide something of interest for everyone who works on T-cell development and activation. I would like to mention that my primary reason for agreeing to put this book together is that, when I was a graduate student, I purchased a copy of Selected Methods in Cellular Immunology by Mishell and Shigii which proved a tremendous help in learning the basics of one-and two- dimensional gel te- niques (and other methods). The cover has long since fallen off, but it still remains one of my most valued reference books for the laboratory. It is my hope that T Cell Protocols: Development and Activation will prove similarly useful to current and future scientists wishing to learn new methods for expl- ing the development and activation of T cells.

Author : Alastair D. Reith
Publisher : Springer Science & Business Media
Page : 367 pages
File Size : 46,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592590599

Get Book

Protein Kinase Protocols by Alastair D. Reith Pdf

As key components of many cell signaling pathways, protein kinases are implicated in a broad variety of diseases, including cancers and neurodegenerative conditions, and offer considerable potential as tractable targets for therapeutic intervention. In Protein Kinase Protocols, a panel of highly skilled laboratory investigators describe both basic and more sophisticated methods for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Described in step-by-step detail, these readily reproducible techniques offer novices quick access to a complicated field, and provide more experienced investigators many novel time-saving ploys. Emphasis is given to the critical technical steps that are often omitted from methods published in the primary literature. There are also tips on potential pitfalls and copious notes on how to adjust the protocols to work in related systems. Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Protocols offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.

Author : Michael H. Gelb
Publisher : Springer Science & Business Media
Page : 243 pages
File Size : 48,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592592647

Get Book

Protein Lipidation Protocols by Michael H. Gelb Pdf

In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a "hands-on" format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered. Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs.

Author : Gregory A. Tucker,Jeremy A. Roberts
Publisher : Springer Science & Business Media
Page : 201 pages
File Size : 54,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592590674

Get Book

Plant Hormone Protocols by Gregory A. Tucker,Jeremy A. Roberts Pdf

Established investigators from around the world describe in step-by-step detail their best techniques for the study of plant hormones and their regulatory activities. These state-of-the-art methods include contemporary approaches to identifying the biosynthetic pathways of plant hormones, monitoring their levels, characterizing the receptors with which they interact, and analyzing the signaling systems by which they exert their effects. Comprehensive and fully detailed for reproducible laboratory success, Plant Hormone Protocols offers plant biologists an indispensable compendium of today's most powerful methods and strategies to studying plant hormones, their regulation, and their activities.

Author : Ambikaipakan Balasubramaniam
Publisher : Springer Science & Business Media
Page : 244 pages
File Size : 45,7 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781592590421

Get Book

Neuropeptide Y Protocols by Ambikaipakan Balasubramaniam Pdf

The observation that neuropeptide Y (NPY) is the most abundant peptide present in the mammalian nervous system and the finding that it elicits the most powerful orexigenic signal have led to active investigations of the properties of the NPY family of hormones, including peptide YY (PYY) and pancreatic polypeptide (PP). Nearly two decades of research have led to the identification of several NPY receptor subtypes and the development of useful receptor selective ligands. Moreover, these investigations have imp- cated NPY in the pathophysiology of a number of diseases, including feeding disorders, seizures, memory loss, anxiety, depression, and heart failure. Vigorous efforts are therefore continuing, not only to understand the bioche- cal aspects of NPY actions, but also toward developing NPY-based treatments for a variety of disorders. To facilitate these efforts, it was decided to produce the first handbook on NPY research techniques as part of the Methods in Molecular Biology Series. In compiling Neuropeptide Y Protocols, I have gathered contributions on techniques considered critical for the advancement of the NPY field from experts in various disciplines. Each chapter starts with a brief introduction, with Materials and Methods sections following. The latter sections are presented in an easy to follow step-by-step format. The last section of the chapter, Notes, highlights pitfalls and the maneuvers employed to overcome them. This information, not usually disseminated in standard research pub- cations, may prove extremely useful for investigators employing these te- niques in NYP research.

Author : Elisabetta Dejana,Monica Corada
Publisher : Springer Science & Business Media
Page : 234 pages
File Size : 50,8 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592592586

Get Book

Adhesion Protein Protocols by Elisabetta Dejana,Monica Corada Pdf

Adhesion molecules are of fundamental importance in the regulation of immunity, inflammation, tissue remodeling, and embryonic development. They comprise different families of homologous proteins, such as selectins, integrins, cadherins, and immunoglobins. In addition, beyond these groups, other str- tures with adhesive properties, such as proteoglycans, occludin, and CD44, have been characterized recently. An understanding of the type and characteristics of adhesive molecules expressed by the different cell types and the possibility of manipulating their activity promises considerable clinical potential. Antibodies, small peptidic and nonpeptidic molecules, have recently been used to inhibit thrombosis by blocking platelet aggregation or inflammation through inhibition of leukocyte infiltration and adhesion. Inhibitors of adhesive molecules are used in expe- mental systems for the study of tumor growth and dissemination. Among major goals in the field are the identification of new members of the known adhesive protein families and of independent new adhesive structures. After structural characterization, even more demanding is the study of the biological activity of the new proteins, and the development of simple, rapid tests for the screening of possible inhibitors. In this regard, the production of such reagents as fragments and antibodies would help define the structure–function relati- ship of individual proteins. Data available in the literature show the complexity of the adhesive process and how different molecular epitopes might contribute to the adhesive properties of a single structure. Finally, a new area of investi- tion is the characterization of the intracellular signaling cascade triggered by the engagement of transmembrane adhesive proteins.

Author : Rocky S. Tuan,Cecilia W. Lo
Publisher : Springer Science & Business Media
Page : 570 pages
File Size : 54,8 Mb
Release : 2000-01-21
Category : Medical
ISBN : 089603576X

Get Book

Developmental Biology Protocols by Rocky S. Tuan,Cecilia W. Lo Pdf

This three-volume set, consisting of 142 chapters, is intentionally broad in scope, because of the nature of modern developmental biology.

Author : Min Li
Publisher : Springer Science & Business Media
Page : 201 pages
File Size : 45,8 Mb
Release : 2008-02-03
Category : Medical
ISBN : 9781592596836

Get Book

NMDA Receptor Protocols by Min Li Pdf

Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane signaling receptors. NMDA Receptor Protocols offers today's scientists powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.

Author : Curtis A. Machida
Publisher : Springer Science & Business Media
Page : 563 pages
File Size : 42,7 Mb
Release : 2008-02-03
Category : Medical
ISBN : 9781592596843

Get Book

Adrenergic Receptor Protocols by Curtis A. Machida Pdf

Adrenergic receptors are important modulators in the sympathetic c- trol of various metabolic processes in the central and peripheral nervous s- tems. These receptors are localized at multiple sites throughout the central nervous system (CNS) and serve as important regulators of CNS-mediated behavior and neural functions, including mood, memory, neuroendocrine c- trol, and stimulation of autonomic function. Adrenergic Receptor Protocols consists of 35 chapters dealing with va- ous aspects of adrenergic receptor analyses, including the use of genetic, RNA, protein expression, transactivator, second messenger, immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches. This volume details the use of various methods to examine the adrenergic receptor system, using aspects of the genetic flow of information as a guide (DNA? RNA ? transactivator ? protein expression ? second messenger analyses ? cellular analyses ? transgenic whole animal approaches). Adrenergic Receptor Protocols displays step-by-step methods for s- cessful replication of experimental procedures, and would be useful for both experienced investigators and newcomers in the field, including those beg- ning graduate study or undergoing postdoctoral training. The Notes section contained in each chapter provides valuable troubleshooting guides to help develop working protocols for your laboratory. With Adrenergic Receptor Protocols, it has been my intent to develop a comprehensive collection of modern molecular methods for analyzing adrenergic receptors. I would like to thank the many chapter authors for their contributions.