Dna Vaccines

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DNA Vaccines

Ângela Sousa
DNA Vaccines Book PDF

Author : Ângela Sousa
Publisher : Humana
Page : 335 pages
File Size : 48,8 Mb
Release : 2021-09-05
Category : Medical
ISBN : 1071608746

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DNA Vaccines by Ângela Sousa Pdf

This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.

DNA Vaccines

Ângela Sousa
DNA Vaccines Book PDF

Author : Ângela Sousa
Publisher : Humana
Page : 0 pages
File Size : 54,8 Mb
Release : 2020-08-22
Category : Medical
ISBN : 1071608711

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DNA Vaccines by Ângela Sousa Pdf

This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.

DNA Vaccines

Douglas B. Lowrie,Robert Whalen
DNA Vaccines Book PDF

Author : Douglas B. Lowrie,Robert Whalen
Publisher : Springer Science & Business Media
Page : 530 pages
File Size : 46,7 Mb
Release : 2008-02-01
Category : Medical
ISBN : 9781592596881

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DNA Vaccines by Douglas B. Lowrie,Robert Whalen Pdf

The field of DNA vaccines has undergone explosive growth in the last few years. As usual, some historical precursors of this approach can be d- cerned in the scientific literature of the last decades. However, the present state of affairs appears to date from observations made discreetly in 1988 by Wolff, Malone, Felgner, and colleagues, which were described in a 1989 patent and published in 1990. Quite surprisingly, they showed that genes carried by pure plasmid DNA and injected in a saline solution, hence the epithet “naked DNA,” could be taken up and expressed by skeletal muscle cells with a low but reproducible frequency. Such a simple methodology was sure to spawn many applications. In a separate and important line of experimentation, Tang, De Vit, and Johnston announced in 1992 that it was indeed possible to obtain humoral immune responses against proteins encoded by DNA delivered to the skin by a biolistic device, which has colloquially become known as the “gene gun. ” The year 1993 saw the publication of further improvements in the me- ods of naked DNA delivery and, above all, the first demonstrations by several groups of the induction of humoral and cytotoxic immune responses to viral antigens expressed from injected plasmid DNA. In some cases, protection against challenge with the pathogen was obtained. The latter result was - questionably the touchstone of a method of vaccination worthy of the name.

DNA Vaccines

Mark W. Saltzman,Hong Shen,Janet L. Brandsma
DNA Vaccines Book PDF

Author : Mark W. Saltzman,Hong Shen,Janet L. Brandsma
Publisher : Springer Science & Business Media
Page : 382 pages
File Size : 46,6 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781597451680

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DNA Vaccines by Mark W. Saltzman,Hong Shen,Janet L. Brandsma Pdf

In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism’s genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s.

DNA Vaccination/Genetic Vaccination

Hilary Koprowski,David B. Weiner
DNA Vaccination Genetic Vaccination Book PDF

Author : Hilary Koprowski,David B. Weiner
Publisher : Springer Science & Business Media
Page : 215 pages
File Size : 44,5 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642804755

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DNA Vaccination/Genetic Vaccination by Hilary Koprowski,David B. Weiner Pdf

Genetic / DNA immunization represents a novel approach to vaccine and immune therapeutic development. The direct injec tion of nucleic acid expression cassettes into a living host results in a limited number of its cells becoming factories for production of the introduced gene products. This host-inappropriate gene expression has important immunological consequences, resulting in the specific immune activation of the host against the gene delivered antigen. The recent demonstration by a number of laboratories that the induced immune responses are functional in experimental models against both specific infectious diseases and cancers is likely to have dramatic consequences for the develop ment of a new generation of experimental vaccines and immune therapies. This technology has the potential to enable the pro duction of vaccines and immune-based therapies that are not only effective immunologically but are accessible to the entire world (rather than just to the most developed nations). Vaccine Development Vaccination against pathogenic microorganisms represents one of the most important advances in the history of medicine. Vaccines, including those against polio, measles, mumps, rubella, hepatitis A, hepatitis B, pertussis and other diseases, have dramatically improved and protected more human lives than any other avenue of modern medicine. The vaccine against smallpox, for example, has been so successful that it is now widely believed that this malicious killer, responsible for more deaths in the twentieth century than World Wars I and II combined, has been removed from the face of the earth.

Opportunities in Biotechnology for Future Army Applications

National Research Council,Division on Engineering and Physical Sciences,Board on Army Science and Technology,Committee on Opportunities in Biotechnology for Future Army Applications
Opportunities in Biotechnology for Future Army Applications Book PDF

Author : National Research Council,Division on Engineering and Physical Sciences,Board on Army Science and Technology,Committee on Opportunities in Biotechnology for Future Army Applications
Publisher : National Academies Press
Page : 118 pages
File Size : 46,9 Mb
Release : 2001-07-11
Category : Science
ISBN : 9780309075558

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Opportunities in Biotechnology for Future Army Applications by National Research Council,Division on Engineering and Physical Sciences,Board on Army Science and Technology,Committee on Opportunities in Biotechnology for Future Army Applications Pdf

This report surveys opportunities for future Army applications in biotechnology, including sensors, electronics and computers, materials, logistics, and medical therapeutics, by matching commercial trends and developments with enduring Army requirements. Several biotechnology areas are identified as important for the Army to exploit, either by direct funding of research or by indirect influence of commercial sources, to achieve significant gains in combat effectiveness before 2025.

DNA Vaccines

Arthur M. Dixon
DNA Vaccines Book PDF

Author : Arthur M. Dixon
Publisher : Nova Science Publishers
Page : 0 pages
File Size : 41,5 Mb
Release : 2011
Category : DNA vaccines
ISBN : 1613244444

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DNA Vaccines by Arthur M. Dixon Pdf

DNA vaccination is one of the most promising techniques for immunisation against diseases caused by viruses, protozoa, bacteria, and even for tumours and illnesses with genetic origins. These vaccines can be administered by direct inoculation of plasmid by several routes. In this book, the authors present topical research in the study of the types, advantages and limitations of DNA vaccines. Topics discussed include the role of novel carrier constructs in effective and safe DNA vaccine delivery; improving the immunogenicity of DNA vaccines; the role of DNA-IL-12 vaccination in cosinphilic inflammation; genetic immunisation enabling re-direction of the host immune system resulting in the development of effective immune responses; and DNA vaccination against herpesvirus.

Gene Vaccination: Theory and Practice

Eyal Raz
Gene Vaccination Theory and Practice Book PDF

Author : Eyal Raz
Publisher : Springer Science & Business Media
Page : 182 pages
File Size : 43,7 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642468674

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Gene Vaccination: Theory and Practice by Eyal Raz Pdf

Vaccination has been established as an efficient procedure to prevent infections. Over the past few years, a new method of subunit vaccination has attracted the attention of immunologists. Despite its popularity, it is only recently that the basic mechanisms that drive the immune response to the encoded antigen have begun to unfold. The multidisciplinary approach of this book outlines the basic characteristics of gene (DNA) vaccination, the role of APCs or bone marrow derived cells in the induction of the immune response. It points out the potential applications for various infectious and allergic diseases and describes the multifaceted properties of DNA in initiating and determining the subsequent immune responses to the encoded antigen.

Vaccine Development and Manufacturing

Emily P. Wen,Ronald Ellis,Narahari S. Pujar
Vaccine Development and Manufacturing Book PDF

Author : Emily P. Wen,Ronald Ellis,Narahari S. Pujar
Publisher : John Wiley & Sons
Page : 452 pages
File Size : 53,8 Mb
Release : 2014-10-06
Category : Technology & Engineering
ISBN : 9781118870907

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Vaccine Development and Manufacturing by Emily P. Wen,Ronald Ellis,Narahari S. Pujar Pdf

Vaccine Manufacturing and Production is an invaluable reference on how to produce a vaccine - from beginning to end - addressing all classes of vaccines from a processing, production, and regulatory viewpoint. It will provide comprehensive information on the various fields involved in the production of vaccines, from fermentation, purification, formulation, to regulatory filing and facility designs. In recent years, there have been tremendous advances in all aspects of vaccine manufacturing. Improved technology and growth media have been developed for the production of cell culture with high cell density or fermentation. Vaccine Manufacturing and Production will serve as a reference on all aspects of vaccine production by providing an in-depth description of the available technologies for making different types of vaccines and the current thinking in facility designs and supply issues. This book will provide insight to the issues scientists face when producing a vaccine, the steps that are involved, and will serve as a reference tool regarding state-of-the-art vaccine manufacturing technologies and facility set-up. Highlights include: Comprehensive coverage of vaccine production : from a process point of view- fermentation to purification to formulation developments; from a production point of view - from facility design to manufacturing; and from a regulatory point of view - requirements from government agencies Authors from different major pharmaceutical and biotechnology companies Describes the challenges and issues involved in vaccine production and manufacturing of the different classes of vaccines, an area not covered by other books currently on the market

Dna Vaccines

Lane Scheiber
Dna Vaccines Book PDF

Author : Lane Scheiber
Publisher : iUniverse
Page : 388 pages
File Size : 47,5 Mb
Release : 2017-07-31
Category : Medical
ISBN : 9781532017223

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Dna Vaccines by Lane Scheiber Pdf

The world is under constant threat of a virus evolving into a global plague. To this point, there is no direct defense against a widespread viral infection. DNA Vaccines: Design of a Gene to Eradicate HIV offers an innovative and new strategy to combat deadly viral infections. Presented is the discovery of a unique identifier for HIV. Analysis of the transcription and translation mechanisms utilized by cells leads to exploring the function of nuclear transcription factor proteins as intracellular hunter-killer molecules aimed specifically at viral genomes. Utilizing amino acid-to-nucleotide binding characteristics, taking into account Watson-Crick binding, Hoogsteen grove binding, and Vander Waals forces, modifications to existing transcription factors binding sites are undertaken. Transcription factors are altered to specifically target the unique identifier of pathogenic viruses. Binding a transcription factor directly to a viral genome is intended to silence the genome, preventing transcription if embedded in the DNA or interfering with translation if the genome is present in a cells cytoplasm as RNA. This text describes the design of an intracellular courier gene technology to make cells defensible against HIV and other lethal pathogenic viral threats.

Toll-Like Receptors (TLRs) and Innate Immunity

Stefan Bauer,Gunther Hartmann
Toll Like Receptors TLRs and Innate Immunity Book PDF

Author : Stefan Bauer,Gunther Hartmann
Publisher : Springer Science & Business Media
Page : 243 pages
File Size : 42,6 Mb
Release : 2007-12-11
Category : Medical
ISBN : 9783540721673

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Toll-Like Receptors (TLRs) and Innate Immunity by Stefan Bauer,Gunther Hartmann Pdf

Overall recent research on TLRs has led to tremendous increase in our understanding of early steps in pathogen recognition and will presumably lead to potent TLR targeting therapeutics in the future. This book reviews and highlights our recent understanding on the function and ligands of TLRs as well as their role in autoimmunity, dendritic cell activation and target structures for therapeutic intervention.

Gene Vaccines

Josef Thalhamer,Richard Weiss,Sandra Scheiblhofer
Gene Vaccines Book PDF

Author : Josef Thalhamer,Richard Weiss,Sandra Scheiblhofer
Publisher : Springer Science & Business Media
Page : 332 pages
File Size : 42,9 Mb
Release : 2011-08-31
Category : Medical
ISBN : 9783709104392

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Gene Vaccines by Josef Thalhamer,Richard Weiss,Sandra Scheiblhofer Pdf

The induction of antigen-specific immune responses after in vivo transfection with expression plasmids has triggered a revolution of vaccine research. After a first hype, evoked by the fascinating options of this method, clinical studies did not reach the ambitious aims and a phase of disillusion ensued. It became obvious that Gene vaccines displayed a weaker immunogenicity in humans than had been observed in the mouse models. Meanwhile these hurdles have been overcome and gene vaccines undergo a renaissance. The present book gives an update of the “world of naked gene vaccines”, namely DNA and RNA vaccines. Its content ranges from general mechanisms, inherent immunostimulatory properties and the vast potential to modulate immune responses, to recent successful clinical studies and approved veterinary gene vaccines. Beyond the state-of-the-art of genetic immunization, the reader will be stimulated with a chapter addressing “burning questions”.

Canadian Immunization Guide

Canada. Comité consultatif national de l'immunisation,Canada. National Advisory Committee on Immunization
Canadian Immunization Guide Book PDF

Author : Canada. Comité consultatif national de l'immunisation,Canada. National Advisory Committee on Immunization
Publisher : Unknown
Page : 392 pages
File Size : 47,6 Mb
Release : 2006
Category : Immunization
ISBN : 0660193922

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Canadian Immunization Guide by Canada. Comité consultatif national de l'immunisation,Canada. National Advisory Committee on Immunization Pdf

The seventh edition of the Canadian Immunization Guide was developed by the National Advisory Committee on Immunization (NACI), with the support ofthe Immunization and Respiratory Infections Division, Public Health Agency of Canada, to provide updated information and recommendations on the use of vaccines in Canada. The Public Health Agency of Canada conducted a survey in 2004, which confi rmed that the Canadian Immunization Guide is a very useful and reliable resource of information on immunization.

Genetic Immunization

Adrian Bot,Constantin A. Bona
Genetic Immunization Book PDF

Author : Adrian Bot,Constantin A. Bona
Publisher : Springer Science & Business Media
Page : 185 pages
File Size : 52,9 Mb
Release : 2013-03-14
Category : Medical
ISBN : 9781475731637

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Genetic Immunization by Adrian Bot,Constantin A. Bona Pdf

Wolf's discovery demonstrating that a reporter gene is expressed in myocytes subsequent to injection of naked DNA, was exploited by immunologists and vaccinologists to develop a new generation of vaccines. This observation galvanized the research and in a short lapse of time, an oceanic volume of knowledge has been accumulated. The research carried out in a variety of animal models showed the efficacy of genetic immunization against viruses, bacteria, and some parasites by the ability to induce a strong priming effect resulting from long-lasting persistence of plasmid as episomes. Furthermore, it was demonstrated that newborn or infant immune unresponsiveness to classical vaccines can be corrected by genetic immunization. The applications of genetic immunization for prophylaxis of infections was extended to immunotherapy, namely, cancerous, auto immune, and allergic diseases. Immunologists have provided pertinent information on the cellular basis of the immune responses elicited by genetic immunization, and molecular biologists have established the molecular basis of intrinsic adjuvant properties of plasmids.

DNA Vaccines

Margaret A. Liu,Maurice R. Hilleman,Reinhard Kurth
DNA Vaccines Book PDF

Author : Margaret A. Liu,Maurice R. Hilleman,Reinhard Kurth
Publisher : Unknown
Page : 320 pages
File Size : 41,6 Mb
Release : 1995
Category : DNA
ISBN : UVA:X004063195

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DNA Vaccines by Margaret A. Liu,Maurice R. Hilleman,Reinhard Kurth Pdf

This conference provides an evaluation of current research on the powerful new technology of DNA vaccines. DNA vaccines hold great promise for prevention and immunotherapy of viral and parasitic infections and cancer, particularly those diseases for which development of traditional vaccines has proved difficult. Preclinical studies recently demonstrated the potential for polynucleotides encoding proteins from pathogens to be a novel and effective means of vaccination, generating cellular and antibody responses while avoiding the potential risks of approaches utilising live viruses.