Electric Orientation Relaxation Flow Linear Dichroism And Circular Dichroism Applied To Dna Complexes With Intercalators And Potentially Multi Intercalating Ligands

Author : Anonim
Publisher : Unknown
Page : 936 pages
File Size : 46,7 Mb
Release : 1986
Category : Sweden
ISBN : UOM:39015055050762

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Svensk bokförteckning by Anonim Pdf

Author : Bonnie Ann Wallace,Robert William Janes
Publisher : IOS Press
Page : 244 pages
File Size : 45,8 Mb
Release : 2009
Category : Medical
ISBN : 9781607500001

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Modern Techniques for Circular Dichroism and Synchrotron Radiation Circular Dichroism Spectroscopy by Bonnie Ann Wallace,Robert William Janes Pdf

Presents an account of circular dichroism (CD) spectroscopy and its application to structural biology. This book covers the methods of synchrotron radiation circular dichroism (SRCD) and linear dichroism (LD).

Author : Alison Rodger,Bengt Nordén
Publisher : Oxford University Press, USA
Page : 170 pages
File Size : 55,7 Mb
Release : 1997
Category : Science
ISBN : 019855897X

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Circular Dichroism and Linear Dichroism by Alison Rodger,Bengt Nordén Pdf

This book provides an introduction to all those who wish to use the complementary spectroscopic techniques of optical activity (circular dichroism, CD) and optical anisotropy (linear dichroism, LD) for the study of the structure of molecules and interactions between molecules in solution. It emphasizes these techniques and how to use them for both low and high molecular weight molecules. The book begins by describing the principles behind CD and LD and how these techniques can be used in the laboratory without using advanced maths or quantum mechanics. The next chapters describe how both techniques may be applied to the study of biological macromolecules and give a detailed description of how they may be used on small molecules to investigate molecular and electronic structure. The final part contains theoretical derivations of all the equations required for the applications described previously. Specific molecular examples are used to illustrate concepts and to show the reader how to use the techniques in chemical and biological systems. Circular Dichroism and Linear Dichroism is an easy guide to what a prospective user of CD needs to know and explains how LD is not merely an exotic technique only to be practiced by experienced spectroscopists, but may be routinely and usefully employed as an aid to molecular structure determination.

Author : Bengt Nordén
Publisher : Lund University Press
Page : 278 pages
File Size : 43,6 Mb
Release : 1977
Category : Science
ISBN : CORNELL:31924004749788

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Linear Dichroism Spectroscopy by Bengt Nordén Pdf

Author : Bengt Nordén,Alison Rodger,Tim Dafforn
Publisher : Royal Society of Chemistry
Page : 305 pages
File Size : 51,5 Mb
Release : 2019-05-02
Category : Science
ISBN : 9781788018210

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Linear Dichroism and Circular Dichroism by Bengt Nordén,Alison Rodger,Tim Dafforn Pdf

This book provides an introduction to optical anisotropy (linear dichroism, LD) and optical activity (circular dichroism, CD) as techniques for the study of structures and interactions of molecules in solution. The book covers the use of these techniques for both small and large molecular systems with particular emphasis being placed on proteins and nucleic acids. CD is a well-established technique and this book aims to explain how it can be used simply and effectively for new entrants to the field as well as covering more advanced techniques for experts. LD is often seen as a rather exotic method intended only for experienced spectroscopists. This book demonstrates that it is an approach with real utility that may be used by both students and scientists from graduate level onwards to give simple answers, which are not available from any other technique, to structural and kinetic questions. Much of the emphasis is on flow orientation of samples in solution phase. The book first describes the techniques and the information they can provide; it then goes on to give specific details on how to actually implement them, including a wide range of examples showing how LD and CD can help with * protein and nucleic acid secondary structure elucidation; * analysis of the formation and rearrangements of fibrous proteins and membrane proteins; * identification of the absolute configuration of small molecules; * determination of the orientation of small molecules in anisotropic media; * assignment of transition moment polarizations; * investigation of binding strengths and geometries of ligand-macromolecule complexes; * 3-D structure determination from LD, molecular replacement and MD modeling. The advantages of combined LD/CD studies are also outlined with examples of DNA/drug complexes and protein insertion into membranes. Taken together the book represents a comprehensive text on the theory and application of LD and CD in the chemical and biological sciences.

Author : Prasad L. Polavarapu
Publisher : CRC Press
Page : 298 pages
File Size : 40,6 Mb
Release : 2016-10-03
Category : Science
ISBN : 9781315357195

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Chiroptical Spectroscopy by Prasad L. Polavarapu Pdf

This book details chiroptical spectroscopic methods: electronic circular dichroism (ECD), optical rotatory dispersion (ORD), vibrational circular dichroism (VCD), and vibrational Raman optical activity (VROA). For each technique, the text presents experimental methods for measurements and theoretical methods for analyzing the experimental data. It also includes a set of experiments that can be adopted for undergraduate teaching laboratories. Each chapter is written in an easy-to-follow format for novice readers, with necessary theoretical formalism in appendices for advanced readers.

Author : John J. Kasianowicz,M. Kellermayer,D DEAMER
Publisher : Springer Science & Business Media
Page : 46 pages
File Size : 45,6 Mb
Release : 2002-07-31
Category : Science
ISBN : 1402006977

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Structure and Dynamics of Confined Polymers by John J. Kasianowicz,M. Kellermayer,D DEAMER Pdf

Polymers are essential to biology because they can have enough stable degrees of freedom to store the molecular code of heredity and to express the sequences needed to manufacture new molecules. Through these they perform or control virtually every function in life. Although some biopolymers are created and spend their entire career in the relatively large free space inside cells or organelles, many biopolymers must migrate through a narrow passageway to get to their targeted destination. This suggests the questions: How does confining a polymer affect its behavior and function? What does that tell us about the interactions between the monomers that comprise the polymer and the molecules that confine it? Can we design and build devices that mimic the functions of these nanoscale systems? The NATO Advanced Research Workshop brought together for four days in Bikal, Hungary over forty experts in experimental and theoretical biophysics, molecular biology, biophysical chemistry, and biochemistry interested in these questions. Their papers collected in this book provide insight on biological processes involving confinement and form a basis for new biotechnological applications using polymers. In his paper Edmund DiMarzio asks: What is so special about polymers? Why are polymers so prevalent in living things? The chemist says the reason is that a protein made of N amino acids can have any of 20 different kinds at each position along the chain, resulting in 20 N different polymers, and that the complexity of life lies in this variety.

Author : Chris R. Calladine,Horace Drew,Ben Luisi,Andrew Travers
Publisher : Elsevier
Page : 352 pages
File Size : 46,8 Mb
Release : 2004-03-13
Category : Science
ISBN : 0080474667

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Understanding DNA by Chris R. Calladine,Horace Drew,Ben Luisi,Andrew Travers Pdf

The functional properties of any molecule are directly related to, and affected by, its structure. This is especially true for DNA, the molecular that carries the code for all life on earth. The third edition of Understanding DNA has been entirely revised and updated, and expanded to cover new advances in our understanding. It explains, step by step, how DNA forms specific structures, the nature of these structures and how they fundamentally affect the biological processes of transcription and replication. Written in a clear, concise and lively fashion, Understanding DNA is essential reading for all molecular biology, biochemistry and genetics students, to newcomers to the field from other areas such as chemistry or physics, and even for seasoned researchers, who really want to understand DNA. Describes the basic units of DNA and how these form the double helix, and the various types of DNA double helix Outlines the methods used to study DNA structure Contains over 130 illustrations, some in full color, as well as exercises and further readings to stimulate student comprehension

Author : Mousumi Debnath,Godavarthi B.K.S. Prasad,Prakash S. Bisen
Publisher : Springer Science & Business Media
Page : 527 pages
File Size : 44,5 Mb
Release : 2010-01-29
Category : Medical
ISBN : 9789048132614

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Molecular Diagnostics: Promises and Possibilities by Mousumi Debnath,Godavarthi B.K.S. Prasad,Prakash S. Bisen Pdf

A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.

Author : Martine Demeunynck,Christian Bailly,W. David Wilson
Publisher : John Wiley & Sons
Page : 754 pages
File Size : 54,8 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527605668

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Small Molecule DNA and RNA Binders by Martine Demeunynck,Christian Bailly,W. David Wilson Pdf

The development of molecules that selectively bind to nucleic acids has provided many details about DNA and RNA recognition. The range of such substances, such as metal complexes, peptides, oligonucleotides and a wide array of synthetic organic compounds, is as manifold as the functions of nucleic acids. Nucleic acid recognition sequences are often found in the major or minor groove of a double strand, while other typical interactions include intercalation between base pairs or the formation of triple or quadruple helices. One example of a binding mode that has recently been proposed is end stacking on such complex structures as the telomere tetraplex. In this comprehensive book, internationally recognized experts describe in detail the important aspects of nucleic acid binding, and in so doing present impressive approaches to drug design. Since typical substances may be created naturally or synthetically, emphasis is placed on natural products, chemical synthesis, the use of combinatorial libraries, and structural characterization. The whole is rounded off by contributions on molecular modeling, as well as investigations into the way in which any given drug interacts with its nucleic acid recognition site.

Author : Giuseppe Spoto,Roberto Corradini
Publisher : Springer Science & Business Media
Page : 320 pages
File Size : 50,9 Mb
Release : 2012-07-06
Category : Science
ISBN : 9789400712263

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Detection of Non-Amplified Genomic DNA by Giuseppe Spoto,Roberto Corradini Pdf

This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.

Author : K. Fukui
Publisher : Springer Science & Business Media
Page : 141 pages
File Size : 42,7 Mb
Release : 2013-04-17
Category : Science
ISBN : 9783642619175

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Theory of Orientation and Stereoselection by K. Fukui Pdf

Many organic chemists will agree with me that the old "electronic theory" has for a long time been inadequate for the interpretation of various new findings in chemistry, particularly for those of reactivity. Considering the outstanding progress which has been made during the past 20 years in the interpretation of these facts, aided by the molecular orbital theory, the time has finally come for a new book showing what is within and what is beyond the reach of quantum-chemical methods. It was therefore highly suitable that Dr. F. L. Boschke of the Springer Verlag suggested to me to make a contribution to a volume in the series "Topics in Current Chemistry" in February 1969. The article was published as Vol. 15, No 1 in June 1970. This new book is an expanded version of the article written in 1970. In this present volume several of the most up-to-date findings which have been gained in organic chemistry since then have been added. It is highly probable that a certain "theoretical" design in the experimenta lists' mind may have been the reason for these developments, whether they themselves are aware of it or not. Theory produces new experimental ideas and conversely, a host of experimental data add another vista to new theories. Due to the mutual beneficial effect of theory and experiment this book will always retain its value, although the quantum-chemical approach to the theory of reactivity is, of course, still in the develop mental stage.

Author : G Michael Blackburn,Michael J Gait,David Loakes,David M Williams
Publisher : Royal Society of Chemistry
Page : 824 pages
File Size : 44,9 Mb
Release : 2015-11-09
Category : Science
ISBN : 9781782625773

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Nucleic Acids in Chemistry and Biology by G Michael Blackburn,Michael J Gait,David Loakes,David M Williams Pdf

The structure, function and reactions of nucleic acids are central to molecular biology and are crucial for the understanding of complex biological processes involved. Revised and updated Nucleic Acids in Chemistry and Biology 3rd Edition discusses in detail, both the chemistry and biology of nucleic acids and brings RNA into parity with DNA. Written by leading experts, with extensive teaching experience, this new edition provides some updated and expanded coverage of nucleic acid chemistry, reactions and interactions with proteins and drugs. A brief history of the discovery of nucleic acids is followed by a molecularly based introduction to the structure and biological roles of DNA and RNA. Key chapters are devoted to the chemical synthesis of nucleosides and nucleotides, oligonucleotides and their analogues and to analytical techniques applied to nucleic acids. The text is supported by an extensive list of references, making it a definitive reference source. This authoritative book presents topics in an integrated manner and readable style. It is ideal for graduate and undergraduates students of chemistry and biochemistry, as well as new researchers to the field.

Author : C. R. Dybowski,Robert L. Lichter
Publisher : Marcel Dekker
Page : 392 pages
File Size : 48,6 Mb
Release : 1987
Category : Science
ISBN : MINN:31951000679290L

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NMR Spectroscopy Techniques by C. R. Dybowski,Robert L. Lichter Pdf

Author : C. N. R. Rao,Achim Müller,Anthony K. Cheetham
Publisher : John Wiley & Sons
Page : 420 pages
File Size : 50,8 Mb
Release : 2007-09-24
Category : Science
ISBN : 9783527611379

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Nanomaterials Chemistry by C. N. R. Rao,Achim Müller,Anthony K. Cheetham Pdf

With this handbook, the distinguished team of editors has combined the expertise of leading nanomaterials scientists to provide the latest overview of this field. They cover the whole spectrum of nanomaterials, ranging from theory, synthesis, properties, characterization to application, including such new developments as quantum dots, nanoparticles, nanoporous materials, nanowires, nanotubes, and nanostructured polymers. The result is recommended reading for everybody working in nanoscience: Newcomers to the field can acquaint themselves with this exciting subject, while specialists will find answers to all their questions as well as helpful suggestions for further research.