Enzyme Kinetics And Mechanism Part D Developments In Enzyme Dynamics

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Enzyme Kinetics and Mechanism Part D

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 44,8 Mb
Release : 1995
Category : Electronic
ISBN : OCLC:760664062

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Enzyme Kinetics and Mechanism Part D by Anonim Pdf

Enzyme Kinetics and Mechanism, Part D: Developments in Enzyme Dynamics

Author : Anonim
Publisher : Academic Press
Page : 0 pages
File Size : 55,8 Mb
Release : 1995-03-22
Category : Science
ISBN : 0121821501

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Enzyme Kinetics and Mechanism, Part D: Developments in Enzyme Dynamics by Anonim Pdf

General Description of the Volume: This volume, as do the other Enzyme Kinetics and Mechanism volumes in the Methods in Enzymology series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed mutagenesis. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Enzyme Kinetics: Catalysis and Control

Author : Daniel L. Purich
Publisher : Elsevier
Page : 920 pages
File Size : 53,6 Mb
Release : 2010-06-16
Category : Science
ISBN : 9780123809254

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Enzyme Kinetics: Catalysis and Control by Daniel L. Purich Pdf

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction Intermediates

Author : Daniel L. Purich
Publisher : Elsevier
Page : 551 pages
File Size : 49,6 Mb
Release : 2002-11-04
Category : Science
ISBN : 9780080489384

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Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction Intermediates by Daniel L. Purich Pdf

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Spectroscopic Detection of Reaction Intermediates Isotopic and Kenetic Detection of Reaction Intermediates Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates

Enzyme Kinetics and Mechanisms, Part E, Energetics of Enzyme Catalysis

Author : Anonim
Publisher : Elsevier
Page : 494 pages
File Size : 51,5 Mb
Release : 1999-09-06
Category : Science
ISBN : 9780080496665

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Enzyme Kinetics and Mechanisms, Part E, Energetics of Enzyme Catalysis by Anonim Pdf

This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Enzyme Kinetics and Mechanisms

Author : Kenneth B. Taylor
Publisher : Springer Science & Business Media
Page : 237 pages
File Size : 44,7 Mb
Release : 2007-05-08
Category : Science
ISBN : 9780306480256

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Enzyme Kinetics and Mechanisms by Kenneth B. Taylor Pdf

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature.

Biothermodynamics

Author : Anonim
Publisher : Academic Press
Page : 369 pages
File Size : 52,9 Mb
Release : 2011-02-16
Category : Science
ISBN : 9780123860040

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Biothermodynamics by Anonim Pdf

The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. * Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules * Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research * Offers step-by-step lab instructions, including necessary equipment, from a global research community

Contemporary Enzyme Kinetics and Mechanism

Author : Anonim
Publisher : Academic Press
Page : 703 pages
File Size : 40,8 Mb
Release : 2009-10-24
Category : Science
ISBN : 9780123847447

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Contemporary Enzyme Kinetics and Mechanism by Anonim Pdf

Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Liposomes, Part D

Author : Anonim
Publisher : Elsevier
Page : 379 pages
File Size : 53,7 Mb
Release : 2004-07-17
Category : Science
ISBN : 9780080522616

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Liposomes, Part D by Anonim Pdf

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes Part D is a continuation of previous Methods in Enzymology Liposome volumes A, B, and C. Covers antibody or ligand targeted liposomes; environment sensitive liposomes; liposomal oligonucleotides; liposomes in vivo

Numerical Computer Methods, Part D

Author : Anonim
Publisher : Elsevier
Page : 527 pages
File Size : 48,9 Mb
Release : 2004-05-25
Category : Computers
ISBN : 9780080497211

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Numerical Computer Methods, Part D by Anonim Pdf

The aim of Numerical Computer Methods, Part D is to brief researchers of the importance of data analysis in enzymology, and of the modern methods that have developed concomitantly with computer hardware. It is also to validate researchers' computer programs with real and synthetic data to ascertain that the results produced are what they expected. Selected Contents: Prediction of protein structure Modeling and studying proteins with molecular dynamics Statistical error in isothermal titration calorimetry Analysis of circular dichroism data Model comparison methods

Energetics of Biological Macromolecules, Part D

Author : Jo M. Holt,Michael L. Johnson,Gary K. Ackers
Publisher : Elsevier
Page : 315 pages
File Size : 49,9 Mb
Release : 2004-04-02
Category : Science
ISBN : 9780080497174

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Energetics of Biological Macromolecules, Part D by Jo M. Holt,Michael L. Johnson,Gary K. Ackers Pdf

This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed. *Methods for Evaluating Cooperativity in a Dimeric Hemoglobin*Multiple-Binding of Ligands to a Linear Biopolymer*Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions*Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis

RNA Polymerase and Associated Factors

Author : Sankar Adhya,Susan Garges
Publisher : Elsevier
Page : 674 pages
File Size : 54,6 Mb
Release : 2003-12-18
Category : Science
ISBN : 0080522602

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RNA Polymerase and Associated Factors by Sankar Adhya,Susan Garges Pdf

RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein. * Models and methods for studying polymerase translocation * Assay for movements of RNA polymerase along DNA * Engineering of elongation complexes of bacterial and yeast RNA polymerases

Nitric Oxide, Part D

Author : Anonim
Publisher : Elsevier
Page : 553 pages
File Size : 40,5 Mb
Release : 2002-12-09
Category : Science
ISBN : 9780080497037

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Nitric Oxide, Part D by Anonim Pdf

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Presents information on nitrosothiols and nitric oxide in cell signaling Details nitric oxide and mitochondrial functions Explains nitric oxide synthases

Enzyme Technology : Pacemaker of Biotechnology

Author : Nooralabettu Krishna Prasad
Publisher : PHI Learning Pvt. Ltd.
Page : 373 pages
File Size : 49,5 Mb
Release : 2011-04
Category : Science
ISBN : 9788120342392

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Enzyme Technology : Pacemaker of Biotechnology by Nooralabettu Krishna Prasad Pdf

Keeping in view the well-established place of enzymes in the field of biotechnology and the recent development in biotech industries, this comprehensive and well-written textbook presents the fundamental concepts of enzyme technology, emphasizing the practical and economic aspects of enzyme usage. Beginning with an overview of enzymes giving insights into the physicochemical properties, classifications, sources, mechanisms and characteristics of enzymes, the text discusses the enzyme kinetics in detail. It furnishes a great deal of information on potential of enzymes for their commercial exploitation. The text then goes on to describe the biotechnical significance of enzymes with their applications in the fields of food and pharmaceutical industries. The text is supported by a large number of solved examples and illustrative diagrams. Primarily designed for undergraduate and postgraduate students of biotechnology and biochemical engineering, the book will also be useful to professionals, researchers and entrepreneurs. KEY FEATURES: Written in an easy-to-understand style. Provides simple, clear and authoritative guide to the principles and scope of enzymes in biotechnology. Includes chapter-end review questions based on recently appeared university question papers.

Guide to Techniques in Mouse Development, Part B

Author : Paul Wassarman,Philippe M Soriano
Publisher : Academic Press
Page : 628 pages
File Size : 43,7 Mb
Release : 2010-09-24
Category : Science
ISBN : 0123848814

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Guide to Techniques in Mouse Development, Part B by Paul Wassarman,Philippe M Soriano Pdf

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. Comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.