Equilibria And Kinetics Of Biological Macromolecules

Author : Prof. Jan Hermans,Barry Lentz
Publisher : John Wiley & Sons
Page : 411 pages
File Size : 55,7 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781118733776

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Equilibria and Kinetics of Biological Macromolecules by Prof. Jan Hermans,Barry Lentz Pdf

Progressively builds a deep understanding of macromolecular behavior Based on each of the authors' roughly forty years of biophysics research and teaching experience, this text instills readers with a deep understanding of the biophysics of macromolecules. It sets a solid foundation in the basics by beginning with core physical concepts such as thermodynamics, quantum chemical models, molecular structure and interactions, and water and the hydrophobic effect. Next, the book examines statistical mechanics, protein-ligand binding, and conformational stability. Finally, the authors address kinetics and equilibria, exploring underlying theory, protein folding, and stochastic models. With its strong emphasis on molecular interactions, Equilibria and Kinetics of Biological Macromolecules offers new insights and perspectives on proteins and other macromolecules. The text features coverage of: Basic theory, applications, and new research findings Related topics in thermodynamics, quantum mechanics, statistical mechanics, and molecular simulations Principles and applications of molecular simulations in a dedicated chapter and interspersed throughout the text Macromolecular binding equilibria from the perspective of statistical mechanics Stochastic processes related to macromolecules Suggested readings at the end of each chapter include original research papers, reviews and monographs, enabling readers to explore individual topics in greater depth. At the end of the text, ten appendices offer refreshers on mathematical treatments, including probability, computational methods, Poisson equations, and defining molecular boundaries. With its classroom-tested pedagogical approach, Equilibria and Kinetics of Biological Macromolecules is recommended as a graduate-level textbook for biophysics courses and as a reference for researchers who want to strengthen their understanding of macromolecular behavior.

Author : Jeffries Wyman,Stanley J. Gill
Publisher : University Science Books
Page : 358 pages
File Size : 50,5 Mb
Release : 1990
Category : Science
ISBN : 0935702563

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Binding and Linkage by Jeffries Wyman,Stanley J. Gill Pdf

Ligand-macromolecule interactions are of fundamental importance in the control of biological processes. This book applies the principles of linkage thermodynamics to polyfunctional macromolecular systems under equilibrium conditions, and describes the binding, linkage, and feedback phenomena that lead to control of complex metabolic processes. The first chapter sets out the different processes (conformational changes, changes in state of aggregation, phase changes) involving biological macromolecules which are affected by chemical variables (such as ligands) or physical variables (such as temperature and pressure). The general effects of ligands on micromolecular conformations and interactions are illustrated with specific examples from the respiratory proteins, electron-transport proteins, and nucleic acid binding proteins. Subsequent chapters develop these themes, and describe in detail how the mathematics of regulation and control can be applied to macromolecules in biological system.

Author : Jo M. Holt,Michael L. Johnson,Gary K. Ackers
Publisher : Elsevier
Page : 315 pages
File Size : 47,7 Mb
Release : 2004-04-02
Category : Science
ISBN : 9780080497174

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Energetics of Biological Macromolecules, Part D by Jo M. Holt,Michael L. Johnson,Gary K. Ackers Pdf

This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed. *Methods for Evaluating Cooperativity in a Dimeric Hemoglobin*Multiple-Binding of Ligands to a Linear Biopolymer*Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions*Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis

Author : Clive R. Bagshaw
Publisher : CRC Press
Page : 446 pages
File Size : 45,8 Mb
Release : 2017-10-04
Category : Medical
ISBN : 9781498727242

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Biomolecular Kinetics by Clive R. Bagshaw Pdf

"a gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide" –Mark Leake, University of York "destined to become a standard reference.... Not just a ‘how to’ handbook but also an accessible primer in the essentials of kinetic theory and practice." –Michael Geeves, University of Kent "covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of transient kinetics and rapid reaction techniques, and then on to the new single molecule techniques" –Stephen Halford, University of Bristol This illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics. Clive R. Bagshaw is Emeritus Professor at the University of Leicester, U.K., and Research Associate at the University of California at Santa Cruz, U.S.A.

Author : Gordon G. Hammes
Publisher : Wiley-Interscience
Page : 192 pages
File Size : 53,6 Mb
Release : 2000-06-26
Category : Science
ISBN : UOM:39015059292105

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Thermodynamics and Kinetics for the Biological Sciences by Gordon G. Hammes Pdf

Gain a working knowledge of thermodynamics and kinetics with a minimum of mathematics-a guide for individuals in the biological sciences An understanding of thermodynamics and kinetics is essential for researchers investigating molecular phenomena in diverse disciplines, including bioorganic chemistry, medicinal chemistry, biochemistry, pharmaceuticals, and biology. The use of these physical chemistry tools in the biological sciences has exploded over the past fifteen years, but the majority of works on thermodynamics and kinetics require mathematical expertise beyond that of many researchers in the field. Presenting a highly accessible introduction to thermodynamics and kinetics, Thermodynamics and Kinetics for the Biological Sciences employs a minimum of mathematics, assuming only a basic calculus background, while treating a wide range of topics in a logical and easy-to-follow style. All principles and concepts are clearly illustrated through the use of relevant applications and examples from the biological sciences, and explanations are further enhanced with problems and up-to-date references. Written by a world-renowned authority on biochemical kinetics, this remarkable book also features an easy-to-understand statistical development of entropy and a more extensive coverage of chemical kinetics and ligand binding to macromolecules than is usually found in books of this kind. Readers will acquire a working knowledge of thermodynamics and kinetics that they can readily apply to biological systems and use for exploring the scientific literature.

Author : James Andrew Goodrich,Jennifer F. Kugel
Publisher : CSHL Press
Page : 188 pages
File Size : 47,6 Mb
Release : 2007
Category : Carrier proteins
ISBN : 9780879697365

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Binding and Kinetics for Molecular Biologists by James Andrew Goodrich,Jennifer F. Kugel Pdf

This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable as an introduction for students. Topics covered range from the basics — such as measuring the concentrations of macromolecules — through considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.

Author : Charles P. Woodbury
Publisher : CRC Press
Page : 272 pages
File Size : 48,6 Mb
Release : 2007-11-08
Category : Science
ISBN : 9781420052992

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Introduction to Macromolecular Binding Equilibria by Charles P. Woodbury Pdf

Macromolecules in the body form noncovalent associations, such as DNA-protein or protein-protein complexes, that control and regulate numerous cellular functions. Understanding how changes in the concentration and conformation of these macromolecules can trigger physiological responses is essential for researchers developing drug therapies to treat

Author : Anonim
Publisher : Elsevier
Page : 581 pages
File Size : 52,5 Mb
Release : 2000-08-09
Category : Science
ISBN : 9780080496788

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Energetics of Biological Macromolecules, Part C by Anonim Pdf

Volume 323 of Methods in Enzymology is dedicated to the energetics of biological macromolecules. Understanding the molecular mechanisms underlying a biological process requires detailed knowledge of the structural relationships within the system and an equally detailed understanding of the energetic driving forces that control the structural interactions. This volume presents modern thermodynamic techniques currently being utilized to study the energetic driving forces in biological systems. It will be a useful reference source and textbook for scientists and students whose goal is to understand the energetic relationships between macromoleculer structures and biological functions. This volume supplements Volumes 259 and Volume 295 of Methods in Enzymology. Key Features * Probing Stability of Helical Transmembrane Proteins * Energetics of Vinca Alkaloid Interactions with Tubulin * Deriving Complex Ligand Binding Formulas * Mathematical Modeling of Cooperative Interactions in Hemoglobin * Analysis of Interactions of Regulatory Protein TyrR with DNA * Parsing Free Energy of Drug-DNA Interactions * Use of Fluorescence as Thermodynamics Tool

Author : Michael L. Johnson,Gary K. Ackers
Publisher : Elsevier
Page : 596 pages
File Size : 44,5 Mb
Release : 1998
Category : Allosteric regulation
ISBN : 012182196X

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Energetics of Biological Macromolecules by Michael L. Johnson,Gary K. Ackers Pdf

Essential publication for researchers in all fields of life sciences. Key Features * Major topics covered include: * Deciphering rules of helix stability in peptides * Protein Folding in Membranes * Molecular Crowding * Study of the Bohr Effect in Hemoglobin Intermediates * Photoacoustic Calorimetry of Proteins * Theoretical Aspects of Isothermal Titration Calorimetry * Energetic Methods to Study Bifunctional Biotin Repressor.

Author : Andrew D. Miller,Julian A. Tanner
Publisher : John Wiley & Sons
Page : 620 pages
File Size : 40,5 Mb
Release : 2013-05-03
Category : Science
ISBN : 9781118687833

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Essentials of Chemical Biology by Andrew D. Miller,Julian A. Tanner Pdf

"This excellent work fills the need for an upper-level graduate course resource that examines the latest biochemical, biophysical, and molecular biological methods for analyzing the structures and physical properties of biomolecules... This reviewer showed [the book] to several of his senior graduate students, and they unanimously gave the book rave reviews. Summing Up: Highly recommended..." CHOICE Chemical biology is a rapidly developing branch of chemistry, which sets out to understand the way biology works at the molecular level. Fundamental to chemical biology is a detailed understanding of the syntheses, structures and behaviours of biological macromolecules and macromolecular lipid assemblies that together represent the primary constituents of all cells and all organisms. The subject area of chemical biology bridges many different disciplines and is fast becoming an integral part of academic and commercial research. This textbook is designed specifically as a key teaching resource for chemical biology that is intended to build on foundations lain down by introductory physical and organic chemistry courses. This book is an invaluable text for advanced undergraduates taking biological, bioorganic, organic and structural chemistry courses. It is also of interest to biochemists and molecular biologists, as well as professionals within the medical and pharmaceutical industry. Key Features: A comprehensive introduction to this dynamic area of chemistry, which will equip chemists for the task of understanding and studying the underlying principles behind the functioning of biological macro molecules, macromolecular lipid assemblies and cells. Covers many basic concepts and ideas associated with the study of the interface between chemistry and biology. Includes pedagogical features such as: key examples, glossary of equations, further reading and links to websites. Clearly written and richly illustrated in full colour.

Author : Keith Moffat,Eaton E. Lattman
Publisher : John Wiley & Sons
Page : 292 pages
File Size : 53,6 Mb
Release : 2024-01-03
Category : Science
ISBN : 9781119696285

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Dynamics and Kinetics in Structural Biology by Keith Moffat,Eaton E. Lattman Pdf

Understand the latest experimental tools in structural biology with this pioneering work Structural biology seeks to understand the chemical mechanisms and functions of biological molecules, such as proteins, based on their atomic structures. Until recently, these structures have been studied only statically, using procedures which deliberately freeze atomic motion. However, freezing eliminates the rapid structural motions so essential to biological activity and function; the molecules are inactive. But with the recent development of X-ray free electron laser (XFEL) sources, efforts to conduct dynamic experiments have expanded using the principles of dynamics and kinetics to capture active biological molecules as they function. Dynamics and Kinetics in Structural Biology promotes the development of these experiments and their successful application. It grounds readers in the foundational principles of dynamics and kinetics; proceeds through extended discussions of experimental procedures, data analysis techniques; and explores experimental frontiers in structural dynamics. The book will aid researchers to gather and interpret cutting-edge data on the dynamic structure of biological molecules, under conditions where they retain their biological functions. Dynamics and Kinetics in Structural Biology offers readers: Authorship by founding figures in the field In-depth presentation of time-resolved X-ray crystallography, solution scattering, and more A pioneering contribution to a rapidly developing field of study Dynamics and Kinetics in Structural Biology is essential reading for graduate students, scientists, researchers and industry professionals engaged in structural studies of biological systems. Industry professionals considering dynamic studies in the development of new product lines will also benefit.

Author : Raymond Chang
Publisher : Macmillan College
Page : 698 pages
File Size : 46,6 Mb
Release : 1981
Category : Science
ISBN : MINN:31951P00424012C

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Physical Chemistry with Applications to Biological Systems by Raymond Chang Pdf

Author : Ambrosius Publishing
Publisher : Future Skill Software
Page : 320 pages
File Size : 55,6 Mb
Release : 2011-03-30
Category : Science
ISBN : 9780982477304

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Enzyme Kinetics for Systems Biology by Ambrosius Publishing Pdf

This book introduces fundamental concepts in kinetics that relate to system biology. The text is suitable for junior/senior undergraduates and graduates who need access to information relevant to modeling biochemical pathways.

Author : T.L. Hill
Publisher : Springer Science & Business Media
Page : 468 pages
File Size : 47,9 Mb
Release : 2013-03-07
Category : Science
ISBN : 9781461250821

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Cooperativity Theory in Biochemistry by T.L. Hill Pdf

During the past few decades we have witnessed an era of remarkable growth in the field of molecular biology. In 1950 very little was known of the chemical constitution of biological systems, the manner in which information was trans mitted from one organism to another, or the extent to which the chemical basis of life is unified. The picture today is dramatically different. We have an almost bewildering variety of information detailing many different aspects of life at the molecular level. These great advances have brought with them some breath-taking insights into the molecular mechanisms used by nature for rep licating, distributing and modifying biological information. We have learned a great deal about the chemical and physical nature of the macromolecular nucleic acids and proteins, and the manner in which carbohydrates, lipids and smaller molecules work together to provide the molecular setting of living sys tems. It might be said that these few decades have replaced a near vacuum of information with a very large surplus. It is in the context of this flood of information that this series of monographs on molecular biology has been organized. The idea is to bring together in one place, between the covers of one book, a concise assessment of the state of the subject in a well-defined field. This will enable the reader to get a sense of historical perspective-what is known about the field today-and a description of the frontiers of research where our knowledge is increasing steadily.

Author : Lev A. Blumenfeld,Alexander N. Tikhonov
Publisher : Springer Science & Business Media
Page : 184 pages
File Size : 43,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781461226307

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Biophysical Thermodynamics of Intracellular Processes by Lev A. Blumenfeld,Alexander N. Tikhonov Pdf

This book is aimed at a large audience: from students, who have a high school background in physics, mathematics, chemistry, and biology, to scien tists working in the fields of biophysics and biochemistry. The main aim of this book is to attempt to describe, in terms of physical chemistry and chemi cal physics, the peculiar features of "machines" having molecular dimen sions which play a crucial role in the most important biological processes, viz., energy transduction and enzyme catalysis. One of the purposes of this book is to analyze the physical background of the high efficiency of molecu lar machines functioning in the living cell. This book begins with a brief review of the subject (Chapter 1). Macro molecular energy-transducing complexes operate with thermal, chemical, and mechanical energy, therefore the appropriate framework to discuss the functioning of biopolymers comes from thermodynamics and chemical kinet ics. That is why we start our analysis with a consideration of the conventional approaches of thermodynamics and classical chemical kinetics, and their application to the description of bioenergetic processes (Chapter 2). Critical analysis of these approaches has led us to the conclusion that the conven tional approaches of physical chemistry to the description of the functioning of individual macromolecular devices, in many cases, appear to be incom plete. This prompted us to consider the general principles ofliving machinery from another point of view."