Developmental Biology Protocols

Author : Rocky S. Tuan,Cecilia W. Lo
Publisher : Springer Science & Business Media
Page : 530 pages
File Size : 47,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592590667

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Developmental Biology Protocols by Rocky S. Tuan,Cecilia W. Lo Pdf

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in “experimental embry- ogy” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Author : Rocky S. Tuan,Cecilia W. Lo
Publisher : Springer Science & Business Media
Page : 537 pages
File Size : 55,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590650

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Developmental Biology Protocols by Rocky S. Tuan,Cecilia W. Lo Pdf

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Author : Rocky S. Tuan,Cecilia W. Lo
Publisher : Humana Press
Page : 505 pages
File Size : 44,5 Mb
Release : 2000-01-17
Category : Science
ISBN : 0896035751

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Developmental Biology Protocols by Rocky S. Tuan,Cecilia W. Lo Pdf

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Author : Rocky S. Tuan,Cecilia W. Lo
Publisher : Humana Press
Page : 0 pages
File Size : 49,7 Mb
Release : 1999-12-01
Category : Science
ISBN : 0896038556

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Developmental Biology Protocols, Volumes I, II, and III by Rocky S. Tuan,Cecilia W. Lo Pdf

The fast-growing field of developmental biology provides a unifying framework that integrates anatomy, physiology, genetics, biochemistry, cellular and molecular biology, as well as evolutionary biology. The number of "reference" publications that deal specifically with the practical aspects of experimental developmental biology are relatively scarce. Developmental Biology Protocols grows out of this need for a comprehensive laboratory manual that provides the readers with the principles, background, rationale, as well as practical protocols, for studying and analyzing the events of embryonic development. Its highly practical format and wide range of model systems and multidisciplinary experimental techniques attest to the authors' determination to provide a balanced presentation of both background information and actual laboratory details. This three-volume compilation of relevant and useful information will be a well-utilized resource for both the students and teachers of developmental biology at all levels. Developmental Biology Protocols offers the most comprehensive, cutting-edge collection of contemporary experimental methods available for the study of embryogenesis and development. Written by an interdisciplinary team of leading scientific investigators, this authoritative collection provides step-by-step instructions for successful laboratory execution. Topics range from animal model system acquisition to molecular genetics, and include high resolution imaging, transgenesis, teratology, comparative anatomy and embryology, the characterization of embryonic structure and function, the analysis of cell function, and the regulation of gene activity. In addition to many unique, new experimental protocols, the authors have also utilized a host of experimental approaches used in other disciplines applicable to the study of development. Comprehensive and richly annotated, Developmental Biology Protocols constitutes the gold-standard reference for today's developmental biologists.

Author : John M. Walker
Publisher : Unknown
Page : 128 pages
File Size : 52,9 Mb
Release : 1984
Category : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
ISBN : LCCN:84015696

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Methods in Molecular Biology: Developmental biology protocols by John M. Walker Pdf

Author : Rocky S. Tuan,Cecilia W. Lo
Publisher : Humana Press
Page : 0 pages
File Size : 41,5 Mb
Release : 1999-11-19
Category : Science
ISBN : 0896038521

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Developmental Biology Protocols by Rocky S. Tuan,Cecilia W. Lo Pdf

Developmental biology is one of the most exciting and fast-growing fields - day. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences alike. Though modern developmental biology has its roots in "experimental embry- ogy" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Author : Anonim
Publisher : Academic Press
Page : 329 pages
File Size : 48,8 Mb
Release : 1997-11-17
Category : Science
ISBN : 0080584594

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Cellular and Molecular Procedures in Developmental Biology by Anonim Pdf

This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. Provides straightforward, current protocols and critical appraisals Includes diverse analysis of cellular and molecular techniques Presents everything from whole-organ cultures to electrophysiological approaches Details a variety of methods for interfering with gene function in various species Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis

Author : Simon G. Sprecher
Publisher : Unknown
Page : 527 pages
File Size : 41,5 Mb
Release : 2019
Category : Animal models in research
ISBN : 1493997327

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Brain Development by Simon G. Sprecher Pdf

This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain.

Author : Laura R. Keller,John Hyde Evans,Thomas C. S. Keller
Publisher : Academic Press
Page : 130 pages
File Size : 44,5 Mb
Release : 1999
Category : Medical
ISBN : 0124039707

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Experimental Developmental Biology by Laura R. Keller,John Hyde Evans,Thomas C. S. Keller Pdf

This work is designed for use as a lab manual in college-level courses in developmental biology or animal development. In each exercise, students examine gametes and developing embryos of a single species, and also perform several experiments to probe its developmental process.

Author : Craig Harris,Jason M. Hansen
Publisher : Humana Press
Page : 484 pages
File Size : 44,8 Mb
Release : 2016-05-01
Category : Medical
ISBN : 1493962078

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Developmental Toxicology by Craig Harris,Jason M. Hansen Pdf

This book details methods used to study developmental toxicology. It highlights the evolution of methods from classical teratology approaches to dynamic, state-of-the-art molecular methods, systems biology, and next generation models and procedures.

Author : Sebastian Dworkin
Publisher : Unknown
Page : 313 pages
File Size : 53,8 Mb
Release : 2022
Category : Facial bones
ISBN : 1071618474

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Craniofacial Development by Sebastian Dworkin Pdf

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. .

Author : David J. Carroll,Stephen A. Stricker
Publisher : Unknown
Page : 347 pages
File Size : 40,8 Mb
Release : 2014
Category : Cytology
ISBN : 1627039740

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Developmental Biology of the Sea Urchin and Other Marine Invertebrates by David J. Carroll,Stephen A. Stricker Pdf

In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates.

Author : Guojun Sheng
Publisher : Humana Press
Page : 365 pages
File Size : 43,8 Mb
Release : 2017-08-15
Category : Science
ISBN : 1493972154

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Avian and Reptilian Developmental Biology by Guojun Sheng Pdf

This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a var iety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.

Author : William Sullivan,M. Ashburner,R. Scott Hawley
Publisher : CSHL Press
Page : 798 pages
File Size : 49,6 Mb
Release : 2000
Category : Medical
ISBN : 0879695862

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Drosophila Protocols by William Sullivan,M. Ashburner,R. Scott Hawley Pdf

This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Author : Monte Westerfield,Leonard I. Zon,H. William Detrich, III
Publisher : Academic Press
Page : 584 pages
File Size : 51,6 Mb
Release : 2009-08-27
Category : Science
ISBN : 9780080923437

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Essential Zebrafish Methods: Cell and Developmental Biology by Monte Westerfield,Leonard I. Zon,H. William Detrich, III Pdf

Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development. This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and “tricks of the trade to both experienced researchers and more junior members in the lab. Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error