Fluorescent Imaging

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Fundamentals of Fluorescence Imaging

Guy Cox
Fundamentals of Fluorescence Imaging Book PDF

Author : Guy Cox
Publisher : CRC Press
Page : 430 pages
File Size : 48,5 Mb
Release : 2019-04-23
Category : Science
ISBN : 9781351129381

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Fundamentals of Fluorescence Imaging by Guy Cox Pdf

Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.

Fluorescent Materials for Cell Imaging

Fu-Gen Wu
Fluorescent Materials for Cell Imaging Book PDF

Author : Fu-Gen Wu
Publisher : Springer Nature
Page : 247 pages
File Size : 43,6 Mb
Release : 2020-10-26
Category : Medical
ISBN : 9789811550621

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Fluorescent Materials for Cell Imaging by Fu-Gen Wu Pdf

This book focuses on the latest fluorescent materials for cell imaging. Cell imaging is a widely used basic technique that helps scientists gain a better understanding of biological functions through studies of cellular structure and dynamics. In the past decades, the development of a variety of new fluorescent materials has significantly extended the applications of cellular imaging techniques. This book presents recently developed fluorescent materials, including semiconductor quantum dots, carbon dots, silicon nanoparticles, metal nanoclusters, upconversion nanoparticles, conjugated polymers/polymer dots, aggregation-induced emission (AIE) probes, and coordination compounds, used for various cellular imaging purposes. It will appeal to cell biologists and other researchers in academia, industry and clinical settings who are interested in the technical development and advanced applications of fluorescence imaging in cells, tissues and organisms to explore the mechanisms of biological functions and diseases.

Fluorescence Microscopy

Ulrich Kubitscheck
Fluorescence Microscopy Book PDF

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Page : 496 pages
File Size : 54,6 Mb
Release : 2017-03-27
Category : Medical
ISBN : 9783527687725

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Fluorescence Microscopy by Ulrich Kubitscheck Pdf

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Fluorescent Imaging

N. Kokudo,T. Ishizawa
Fluorescent Imaging Book PDF

Author : N. Kokudo,T. Ishizawa
Publisher : Karger Medical and Scientific Publishers
Page : 134 pages
File Size : 42,9 Mb
Release : 2013-09-10
Category : Medical
ISBN : 9783318022933

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Fluorescent Imaging by N. Kokudo,T. Ishizawa Pdf

Indocyanine green (ICG) fluorescence has been used for imaging purposes for more than half a century; First employed by ophthalmologists for visualizing the retinal artery in the late 1960s, the application of ICG fluorescence imaging has since been continuously expanded. Recently, advances in imaging technologies have led to renewed attention regarding the use of ICG in the field of hepatobiliary surgery, as a new tool for visualizing the biliary tree and liver tumors.

Fluorescent Imaging in Medicinal Chemistry

Zhen Cheng
Fluorescent Imaging in Medicinal Chemistry Book PDF

Author : Zhen Cheng
Publisher : Springer Nature
Page : 230 pages
File Size : 45,9 Mb
Release : 2020-05-28
Category : Science
ISBN : 9783030467074

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Fluorescent Imaging in Medicinal Chemistry by Zhen Cheng Pdf

This book reviews the most recent developments of fluorescent imaging techniques for medicinal chemistry research and biomedical applications, including cell imaging, in vitro diagnosis and in vivo imaging. Fluorescent imaging techniques play an important role in basic research, drug discovery and clinical translation. They have great impact to many fields including chemical biology, cell biology, medical imaging, cancer diagnosis and treatment, pharmaceutical science, among others, and they have facilitated our understanding of diseases and helped to develop many novel powerful tools for imaging and treatment of diseases. This book will appeal to scientists from numerous fields such as chemistry, pharmaceutical science, biology, materials science, and medicine, and it will serve as a very useful and handy resource for readers with different levels of scientific knowledge, ranging from entry level to professional level.

Fluorescence Microscopy

Anda Cornea,P. Michael Conn
Fluorescence Microscopy Book PDF

Author : Anda Cornea,P. Michael Conn
Publisher : Elsevier
Page : 261 pages
File Size : 51,9 Mb
Release : 2014-02-24
Category : Science
ISBN : 9780124167131

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Fluorescence Microscopy by Anda Cornea,P. Michael Conn Pdf

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

ICG Fluorescence Imaging and Navigation Surgery

Mitsuo Kusano,Norihiro Kokudo,Masakazu Toi,Masaki Kaibori
ICG Fluorescence Imaging and Navigation Surgery Book PDF

Author : Mitsuo Kusano,Norihiro Kokudo,Masakazu Toi,Masaki Kaibori
Publisher : Springer
Page : 474 pages
File Size : 41,5 Mb
Release : 2016-03-11
Category : Medical
ISBN : 9784431555285

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ICG Fluorescence Imaging and Navigation Surgery by Mitsuo Kusano,Norihiro Kokudo,Masakazu Toi,Masaki Kaibori Pdf

This book presents a comprehensive overview and outlook for the future of indocyanine green (ICG) fluorescence navigation surgery, which is attracting clinical interest as a safe and less invasive procedure not only in detecting cerebral vessels, coronary arteries, and biliary trees, but also in identifying sentinel lymph nodes in cancer. The book starts with the characteristics of ICG and photodynamic cameras/endoscopes, followed by detailed descriptions of the applications of ICG fluorescence imaging in various areas such as ocular surgery, neurosurgery, cardiovascular surgery, and plastic surgery. It also covers identifying sentinel lymph nodes in breast cancer as well as cancers of the gastrointestinal tract, and provides valuable information for hepato-biliary-pancreatic surgeons, such as identifying tattooing of liver segments and bile leakage. Written entirely by experts in their respective areas, ICG Fluorescence Imaging and Navigation Surgery offers an essential resource for surgeons operating on cancers and vascular disorders in the brain and cardiovascular systems and in plastic surgery.

Handbook of Fluorescence Spectroscopy and Imaging

Markus Sauer,Johan Hofkens,Jörg Enderlein
Handbook of Fluorescence Spectroscopy and Imaging Book PDF

Author : Markus Sauer,Johan Hofkens,Jörg Enderlein
Publisher : John Wiley & Sons
Page : 425 pages
File Size : 48,9 Mb
Release : 2010-12-23
Category : Science
ISBN : 9783527633524

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Handbook of Fluorescence Spectroscopy and Imaging by Markus Sauer,Johan Hofkens,Jörg Enderlein Pdf

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Fluorescence Imaging for Surgeons

Fernando D. Dip,Takeaki Ishizawa,Norihiro Kokudo,Raul J. Rosenthal
Fluorescence Imaging for Surgeons Book PDF

Author : Fernando D. Dip,Takeaki Ishizawa,Norihiro Kokudo,Raul J. Rosenthal
Publisher : Springer
Page : 360 pages
File Size : 41,9 Mb
Release : 2015-07-21
Category : Medical
ISBN : 9783319156781

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Fluorescence Imaging for Surgeons by Fernando D. Dip,Takeaki Ishizawa,Norihiro Kokudo,Raul J. Rosenthal Pdf

This text presents the experiences of leading researchers and surgeons with different fluorescence methods. Chapters range from basic science of fluorescence to current clinical applications and new horizons. The first few chapters describe the historical evolution and physical principles of fluorescence and provide the foundation for the reader to understand the current scope and limits of its use in surgery. The second section focuses on the clinical applications of intraoperative fluorescence imaging including subsections on fluorescence cholangiography, applications to hepatectomy, lymph node navigation, applications to GI tract and pelvic surgery and identification of cancer tissues. The third section focuses on new frontiers including fluorescence probes, imaging systems and applications to photodynamic therapy. Authored by leaders in the development of fluorescent methods worldwide, Fluorescence Imaging for Surgeons: Concepts and Applications will have an impact on numerous medical specialists including general surgeons, colorectal and minimally-invasive surgeons and surgical oncologists. Researchers will find the book to be an invaluable resource on the latest advances in the utilization of nanoparticles and fluorescent probes.​

Fluorescence Lifetime Spectroscopy and Imaging

Laura Marcu,Paul M. W. French,Daniel S. Elson
Fluorescence Lifetime Spectroscopy and Imaging Book PDF

Author : Laura Marcu,Paul M. W. French,Daniel S. Elson
Publisher : CRC Press
Page : 554 pages
File Size : 51,7 Mb
Release : 2014-07-17
Category : Science
ISBN : 9781439861684

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Fluorescence Lifetime Spectroscopy and Imaging by Laura Marcu,Paul M. W. French,Daniel S. Elson Pdf

During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia

Fluorescence Imaging - Recent Advances and Applications

Raffaello Papadakis
Fluorescence Imaging Recent Advances and Applications Book PDF

Author : Raffaello Papadakis
Publisher : BoD – Books on Demand
Page : 110 pages
File Size : 50,6 Mb
Release : 2023-11-22
Category : Science
ISBN : 9781803551838

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Fluorescence Imaging - Recent Advances and Applications by Raffaello Papadakis Pdf

Fluorescence imaging is widely used in scientific fields ranging from biology to biomedicine and even materials science. The development of novel fluorescent labels and microscopy techniques has rendered fluorescence imaging profoundly useful. Particularly in bioscience, fluorescence imaging empowers the study of the intracellular distribution, dynamics, gene expression, protein–protein interactions, and protein localization and enables the identification and tracking of lysosomes. Fluorescence imaging is applicable in cells and tissues and is constantly gaining attention in medicine too, in the fields of fluorescence-guided surgery and robotic-assisted fluorescence surgery. Acknowledging all these important new trends, this book provides an overview of the recent advances and applications in fluorescence imaging.

Fluorescence Microscopy and Fluorescent Probes

J. Slavík
Fluorescence Microscopy and Fluorescent Probes Book PDF

Author : J. Slavík
Publisher : Springer Science & Business Media
Page : 292 pages
File Size : 41,7 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781489918666

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Fluorescence Microscopy and Fluorescent Probes by J. Slavík Pdf

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Fluorescence Imaging and Biological Quantification

Raquel Seruca,Jasjit S. Suri,J. Miquel Sanches
Fluorescence Imaging and Biological Quantification Book PDF

Author : Raquel Seruca,Jasjit S. Suri,J. Miquel Sanches
Publisher : CRC Press
Page : 376 pages
File Size : 52,9 Mb
Release : 2017-09-06
Category : Medical
ISBN : 9781351647335

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Fluorescence Imaging and Biological Quantification by Raquel Seruca,Jasjit S. Suri,J. Miquel Sanches Pdf

This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.

Fundamentals of Fluorescence Microscopy

Partha Pratim Mondal,Alberto Diaspro
Fundamentals of Fluorescence Microscopy Book PDF

Author : Partha Pratim Mondal,Alberto Diaspro
Publisher : Springer Science & Business Media
Page : 218 pages
File Size : 49,8 Mb
Release : 2013-12-12
Category : Science
ISBN : 9789400775459

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Fundamentals of Fluorescence Microscopy by Partha Pratim Mondal,Alberto Diaspro Pdf

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Fluorescent and Luminescent Probes for Biological Activity

W. T. Mason
Fluorescent and Luminescent Probes for Biological Activity Book PDF

Author : W. T. Mason
Publisher : Elsevier
Page : 647 pages
File Size : 52,9 Mb
Release : 1999-04-16
Category : Science
ISBN : 9780080531779

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Fluorescent and Luminescent Probes for Biological Activity by W. T. Mason Pdf

The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy