Freeze Fracture Images Of Cells And Tissues

Author : Richard L. Roberts,Richard G. Kessel,Hai-Nan Tung
Publisher : Oxford University Press, USA
Page : 430 pages
File Size : 40,7 Mb
Release : 1991
Category : Electron microscopy
ISBN : 0195052285

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Freeze Fracture Images of Cells and Tissues by Richard L. Roberts,Richard G. Kessel,Hai-Nan Tung Pdf

The freeze fracture technique coupled with transmission electron microscopy has contributed significant scientific information toward the experimental investigation of both normal and abnormal cells in the fields of cellular, developmental and molecular biology, and to several subdisciplines in medicine, including pathology, anatomy, and physiology. This book presents a complete and up-to-date account of the macromolecular organization of membranes and the many membrane specialization of cells as well as overall cellular organization as reflected in tissues and organs. While the book emphasizes freeze fracture images and the useful scientific information contained in them, the authors have also included transmission electron micrographs of ultrathin sectioned cells, tissues, and organs in order to aid in the interpretation of the freeze fracture image and increase the book's utility. Where three dimensional views are particularly useful, scanning electron micrographs are included.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 46,8 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Delmas J. Allen,Pietro M. Motta,Liberato J. A. DiDio
Publisher : Elsevier
Page : 383 pages
File Size : 41,5 Mb
Release : 2014-06-28
Category : Science
ISBN : 9781483281155

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Three Dimensional Microanatomy of Cells and Tissue Surfaces by Delmas J. Allen,Pietro M. Motta,Liberato J. A. DiDio Pdf

Three Dimensional Microanatomy of Cells and Tissue Surfaces focuses on the use of scanning electron microscopy in the study of the microanatomy of cells and tissues, cell relationships, and complex biological relationships. The selection first elaborates on the technical aspects of stereoprojection for electron microscopy; three-dimensional microanatomy of intracellular structures; microcirculation studies by the injection-replica method with special reference to portal circulations; and three-dimensional architecture of the mammalian liver. Discussions focus on the preparation of vascular casts, portal circulations of various organs, scanning electron microscopy, copying and printing stereopair negatives, stereoprojection, and high voltage electron microscopy. The text then takes a look at scanning electron microscope bloodvessel casts analysis, three dimensional microanatomy of reticular tissues, kidney glomerular epithelium in response to different physiological states and experimental conditions, and mammalian renal papilla and pelvis. The manuscript examines the lung in scanning electron microscopy and stereopresentation, surface topography of endocardial endothelium, scanning electron microscopy of endothelium, human vas deferens, and seminal vesicles, and dynamic morphology of the apical membrane of lactating cells viewed by freeze-fracture. The selection is a valuable reference for researchers interested in the use of scanning electron microscopy in the study of the microanatomy of cells and tissues and biological relationships.

Author : Heide Schatten
Publisher : Cambridge University Press
Page : 275 pages
File Size : 48,9 Mb
Release : 2013
Category : Science
ISBN : 9780521195997

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Scanning Electron Microscopy for the Life Sciences by Heide Schatten Pdf

A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.

Author : J.K. Koehler
Publisher : Springer Science & Business Media
Page : 319 pages
File Size : 50,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642654923

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Advanced Techniques in Biological Electron Microscopy by J.K. Koehler Pdf

The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.

Author : John E. Rash,C. Sue Hudson
Publisher : Unknown
Page : 226 pages
File Size : 43,5 Mb
Release : 1979
Category : Science
ISBN : UOM:39015000293806

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Freeze Fracture by John E. Rash,C. Sue Hudson Pdf

Author : Roland A. Fleck,Bruno M. Humbel
Publisher : John Wiley & Sons
Page : 741 pages
File Size : 54,7 Mb
Release : 2019-04-29
Category : Science
ISBN : 9781118654064

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Biological Field Emission Scanning Electron Microscopy, 2 Volume Set by Roland A. Fleck,Bruno M. Humbel Pdf

The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

Author : P. Motta
Publisher : Springer Science & Business Media
Page : 350 pages
File Size : 46,6 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461338611

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Ultrastructure of Endocrine Cells and Tissues by P. Motta Pdf

Innovative microscopic techniques, introduced during the last two decades, have contributed much to creating a new picture of the dynamic architecture of the cell, which can now be more exactly correlated with specific biochemical and physiopathological events. These developments have led to significant advances in our understanding of the physiomorphological and pathological aspects of the secretory mechanism, as well as the pharmacologic methods used to control, experimentally, the function of exocrine and endocrine glands. The integration of new ultrastructural methods such as freeze-fracture/etching, immunocytochemistry, scanning and high-voltage electron microscopy, cytoautoradiography, etc. , has proven to be of great value when applied to the study of endocrine cells and tissues. Because information on this topic has appeared in a variety of scientific and medical journals, this book: (1) reviews the results of an integrative approach presenting a comprehensive ultrastructural account of the main aspects of the field; (2) points out gaps or controversial topics in our knowledge; and (3) outlines pertinent directions for future research. The chapters, prepared by recognized authorities in the field, present traditional information on the topic in a concise manner and, with a valuable selection of original illustrations, show what the integration of new microscopic methods can contribute to the subject in terms of new concepts. This volume will be useful to cell biologists, anatomists, embryologists, histologists, pharmacologists, pathologists, and, of course, endocrinologists. It will also be of interest to students, practitioners of medicine, and to all others dealing with clinical research and diagnosis.

Author : Cedric M. Blouin
Publisher : Unknown
Page : 218 pages
File Size : 50,6 Mb
Release : 2020
Category : Cell membranes
ISBN : 1071607324

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Caveolae by Cedric M. Blouin Pdf

This volume explores techniques used to study either the structure or the functions of caveolae and their components in several normal and pathophysiological situations. The chapters in this book cover topics such as selective visualization of caveolae by electron microscopy techniques; spatiotemporal analysis of caveolae dynamics and mechanics using live cell fluorescence microscopy; in vitro reconstitution such as liposomes and GPMVs as tools to study caveolin- and cavin-interacting partners; pulling of tethers from the cell plasma membrane using optical tweezers; and immunofluorescence-based analysis of caveolin-3 in the diagnostic management of neuromuscular diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Caveolae: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and proteins composing their coat in various model organisms. .

Author : Gerald Karp
Publisher : John Wiley & Sons
Page : 836 pages
File Size : 53,7 Mb
Release : 2009-10-19
Category : Science
ISBN : 9780470483374

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Cell and Molecular Biology by Gerald Karp Pdf

Karp continues to help biologists make important connections between key concepts and experimentation. The sixth edition explores core concepts in considerable depth and presents experimental detail when it helps to explain and reinforce the concepts. The majority of discussions have been modified to reflect the latest changes in the field. The book also builds on its strong illustration program by opening each chapter with “VIP” art that serves as a visual summary for the chapter. Over 60 new micrographs and computer-derived images have been added to enhance the material. Biologists benefit from these changes as they build their skills in making the connection.

Author : Sek Wen Hui
Publisher : CRC Press
Page : 375 pages
File Size : 49,7 Mb
Release : 2018-01-18
Category : Medical
ISBN : 9781351089050

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Freeze-Fracture Studies of Membranes by Sek Wen Hui Pdf

This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.

Author : Jianghui Hou
Publisher : Academic Press
Page : 422 pages
File Size : 40,8 Mb
Release : 2020-04-24
Category : Science
ISBN : 9780128226438

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A Laboratory Guide to the Tight Junction by Jianghui Hou Pdf

A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels

Author : Rudolf A. Steinbrecht,Karl Zierold
Publisher : Springer Science & Business Media
Page : 308 pages
File Size : 52,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642728150

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Cryotechniques in Biological Electron Microscopy by Rudolf A. Steinbrecht,Karl Zierold Pdf

To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Author : John J. Bozzola,Lonnie Dee Russell
Publisher : Jones & Bartlett Learning
Page : 702 pages
File Size : 48,7 Mb
Release : 1999
Category : Medical
ISBN : 0763701920

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Electron Microscopy by John J. Bozzola,Lonnie Dee Russell Pdf

New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR

Author : John Kuo
Publisher : Humana
Page : 0 pages
File Size : 44,8 Mb
Release : 2016-08-27
Category : Medical
ISBN : 1493960733

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Electron Microscopy by John Kuo Pdf

This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.