Freeze Fracture Studies Of Membranes

Author : Sek Wen Hui
Publisher : CRC Press
Page : 229 pages
File Size : 51,9 Mb
Release : 2018-01-18
Category : Medical
ISBN : 9781351080606

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Freeze-Fracture Studies of Membranes by Sek Wen Hui Pdf

This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.

Author : John E. Rash,C. Sue Hudson
Publisher : Unknown
Page : 226 pages
File Size : 55,8 Mb
Release : 1979
Category : Science
ISBN : UOM:39015000293806

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Freeze Fracture by John E. Rash,C. Sue Hudson Pdf

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 45,5 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : C. Stolinski,A. S. Breathnach
Publisher : Unknown
Page : 192 pages
File Size : 44,6 Mb
Release : 1975
Category : Medical
ISBN : UOM:39015000286024

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Freeze-fracture Replication of Biological Tissues by C. Stolinski,A. S. Breathnach Pdf

Author : J.K. Koehler
Publisher : Springer Science & Business Media
Page : 319 pages
File Size : 52,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642654923

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Advanced Techniques in Biological Electron Microscopy by J.K. Koehler Pdf

The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.

Author : Nicholas J. Severs,David M. Shotton
Publisher : Wiley-Liss
Page : 392 pages
File Size : 55,9 Mb
Release : 1995-08-11
Category : Medical
ISBN : UOM:39015037496695

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Rapid Freezing, Freeze Fracture, and Deep Etching by Nicholas J. Severs,David M. Shotton Pdf

The techniques of freeze fracture and deep etching—techniques for the examination of the ultrastructure of rapidly frozen biological specimens by transmission electron microscopy—have profoundly influenced our understanding of the functional organization of the cell. Rapid Freezing, Freeze Fracture, and Deep Etching guides the reader through the principles of these techniques and gives detailed examples of their application in biomedical research. The book is organized into three sections. The first covers the fundamentals of freeze fracture and deep etching, with a discussion of basic principles for those new to the field, and an entire chapter on the equipment currently available for freeze fracture. Advanced techniques are presented next, with each chapter devoted to an in-depth treatment of a specific topic, including the advantages and limitations of each technique. The final chapters offer a series of applications selected to illustrate the wide-ranging and enduring impact of rapid freezing, freeze fracture, and deep etching on the field of biomedical research. With over 150 illustrations and containing contributions by leading experts instrumental in the development of these techniques, Rapid Freezing, Freeze Fracture, and Deep Etching offers authoritative coverage of such novel topics as: Hyperbaric freezing Ultrarapid freezing techniques in the study of dynamic cellular processes Cytochemical methods utilizing lectins and antibodies for labeling specific cellcomponents Fracturing and shadowing under ultra-low temperature and ultra-high vacuum conditions Freeze fracture and deep etching in neurobiology Rapid Freezing, Freeze Fracture, and Deep Etching will appeal to both newcomers to the field and experienced researchers and will be essential reading for cell biologists and anatomists, as well as any researcher who uses electron microscopy.

Author : Parvez I. Haris,Dennis Chapman
Publisher : Unknown
Page : 288 pages
File Size : 41,7 Mb
Release : 1998
Category : Science
ISBN : UOM:39015043784886

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Biomembrane Structures by Parvez I. Haris,Dennis Chapman Pdf

Author : William Stillwell
Publisher : Newnes
Page : 378 pages
File Size : 49,5 Mb
Release : 2013-04-20
Category : Science
ISBN : 9780080931289

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An Introduction to Biological Membranes by William Stillwell Pdf

An Introduction to Biological Membranes: From Bilayers to Rafts covers many aspects of membrane structure/function that bridges membrane biophysics and cell biology. Offering cohesive, foundational information, this publication is valuable for advanced undergraduate students, graduate students and membranologists who seek a broad overview of membrane science. Brings together different facets of membrane research in a universally understandable manner Emphasis on the historical development of the field Topics include membrane sugars, membrane models, membrane isolation methods, and membrane transport.

Author : Edward D. Korn
Publisher : Springer Science & Business Media
Page : 267 pages
File Size : 55,5 Mb
Release : 2013-11-21
Category : Science
ISBN : 9781475758207

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Methods in Membrane Biology by Edward D. Korn Pdf

The contributions of electron microscopy to membrane biology have been indispensable and, at the same time, disappointing. Membranes were known to exist before the advent of electron microscopy and general principles of their composition and molecular organization had been deduced from permeability and electrical conductivity measurements, polarized light microscopy, and X-ray diffraction. On the other hand, the complexities of the many intracellular membranes and membranous organelles were really not suspected until they were observed by the electron microscopist. One then had further hopes that the high resolution of the electron microscope (theoretically it can resolve atomic distances) would allow the visualization of the molecular architecture of membranes and lead directly to an under standing of structure and function at the molecular level. This expectation has been largely unrealized. Because of the great difficulties encountered in the preparation of biological material, because of the uncertainty of the chemistry of "staining," and because of numerous electron optical artifacts, it has been a major challenge just to rationalize the observed images in terms of the known facts, let alone to utilize the images to expand our knowledge of the molecular structure of biological membranes. The many differences among membranes with respect to function and composition are lost in the universal trilamellar image. Perhaps the one major exception to this, and the major structural contribution of electron microscopy at the molecular level, has come from freeze-etch electron microscopy.

Author : Ronald Aloia
Publisher : Elsevier
Page : 341 pages
File Size : 55,8 Mb
Release : 2012-12-02
Category : Technology & Engineering
ISBN : 9780323155212

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Concepts of Membrane Structure by Ronald Aloia Pdf

Membrane Fluidity in Biology, Volume 1: Concepts of Membrane Structure covers membrane properties influenced by alterations in membrane lipid compositions and/or other organizational parameters that are encompassed by the term fluidity. This book is composed of eight chapters that discuss significance of fluidity changes in both normal and pathological cellular functions. This book starts by describing membrane structural organization and composition and arrangement of the molecular components of cell membranes. This is followed by discussions on structural properties of lipids and role of nonbilayer lipid structures in membrane fusion. The methodological approaches in study of cellular membrane structural diversity and fluid mosaic model for accurate representation of membrane fluidity are also discussed. This volume then describes the phenomenon of reversed or ""negative"" membrane images, as viewed with transmission electron microscope. Chapters 6 and 7 explain the interaction of cytochrome P-450 with phospholipids and proteins in the endoplasmic reticulum and steps in the derivation of membrane structure and packing principles. Finally, the concluding chapter focuses on the membrane of the human red blood cell and presents relatively simple arguments concerning its physical properties. The book will serve as a primary source for research scientists and teachers interested in cellular membrane fluidity phenomena.

Author : T.E. Andreoli
Publisher : Springer Science & Business Media
Page : 474 pages
File Size : 50,5 Mb
Release : 2013-04-17
Category : Science
ISBN : 9781475717181

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Membrane Physiology by T.E. Andreoli Pdf

Membrane Physiology is a soft-cover book containing portions of Physiology of Membrane Disorders, published in larger, hard-cover form in 1978. The parent volume was divided into five parts, described in detail in the Preface to the hard-cover edition, which is reproduced in this volume. The present version of Membrane Physiology incorporates the first three of these parts, including a section on the Nature of Biological Membranes, a section on Methods for Studying Membranes, and a section on General Problems in Membrane Biology. It is the hope of the Editors that this smaller volume will be of value to individuals interested in general physiology, the methods for studying general physiology, and its potential application to problems of clinical and physiological relevance. The Preface to Physiology of Membrane Disorders indicates our general reasoning for developing such a volume. THOMAS E. ANDREOLI JOSEPH F. HOFFMAN DARRELL D. FANESTIL Vll Preface to Physiology of Membrane Disorders The purpose of this book is to provide the reader with a rational frame of reference for assessing the pathophysiology of those disorders in which derangements of membrane transport processes are a major factor responsible for the clinical manifestations of disease.

Author : Thomas E. Andreoli,Darrell D. Fanestil,Joseph F. Hoffman,Stanley G. Schultz
Publisher : Springer Science & Business Media
Page : 1062 pages
File Size : 48,7 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781461320975

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Physiology of Membrane Disorders by Thomas E. Andreoli,Darrell D. Fanestil,Joseph F. Hoffman,Stanley G. Schultz Pdf

The second edition of Physiology of Membrane Disorders represents an extensive revision and a considerable expansion of the first edition . Yet the purpose ofthe second edition is identical to that of its predecessor, namely, to provide a rational analysis of membrane transport processes in individual membranes, cells, tissues, and organs, which in tum serves as a frame of reference for rationalizing disorders in which derangements of membrane transport processes playa cardinal role in the clinical expression of disease. As in the first edition, this book is divided into a number of individual, but closely related, sections. Part V represents a new section where the problem of transport across epithelia is treated in some detail. Finally, Part VI, which analyzes clinical derangements, has been enlarged appreciably. THE EDITORS xi Preface to the First Edition The purpose of this book is to provide the reader with a rational frame of reference for assessing the pa thophysiology of those disorders in which derangements of membrane transport processes are a major factor responsible for the clinical manifestations of disease. In the present context, we use the term "membrane transport to refer to those molecular processes whose cardinal function, broadly speaking, is processes" in a catholic sense, the vectorial transfer of molecules-either individually or as ensembles-across biological interfaces, the latter including those interfaces which separate different intracellular compartments, the cellular and extracellular com partments, and secreted fluids-such as glomerular filtrate-and extracellular fluids.

Author : Richard L. Roberts,Richard G. Kessel,Hai-Nan Tung
Publisher : Oxford University Press, USA
Page : 430 pages
File Size : 40,8 Mb
Release : 1991
Category : Electron microscopy
ISBN : 0195052285

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Freeze Fracture Images of Cells and Tissues by Richard L. Roberts,Richard G. Kessel,Hai-Nan Tung Pdf

The freeze fracture technique coupled with transmission electron microscopy has contributed significant scientific information toward the experimental investigation of both normal and abnormal cells in the fields of cellular, developmental and molecular biology, and to several subdisciplines in medicine, including pathology, anatomy, and physiology. This book presents a complete and up-to-date account of the macromolecular organization of membranes and the many membrane specialization of cells as well as overall cellular organization as reflected in tissues and organs. While the book emphasizes freeze fracture images and the useful scientific information contained in them, the authors have also included transmission electron micrographs of ultrathin sectioned cells, tissues, and organs in order to aid in the interpretation of the freeze fracture image and increase the book's utility. Where three dimensional views are particularly useful, scanning electron micrographs are included.

Author : Roland C. Aloia,Cyril C. Curtain,Larry M. Gordon
Publisher : Unknown
Page : 352 pages
File Size : 51,6 Mb
Release : 1988
Category : Cell Membrane
ISBN : UOM:39015013203537

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Methods for Studying Membrane Fluidity by Roland C. Aloia,Cyril C. Curtain,Larry M. Gordon Pdf

Author : A.N. Martonosi
Publisher : Springer Science & Business Media
Page : 467 pages
File Size : 46,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468445985

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The Enzymes of Biological Membranes by A.N. Martonosi Pdf

In the first edition of The Enzymes of Biological Membranes, published in four volumes in 1976, we collected the mass of widely scattered information on membrane-linked enzymes and metabolic processes up to about 1975. This was a period of transition from the romantic phase of membrane biochemistry, preoccupied with conceptual developments and the general properties of membranes, to an era of mounting interest in the specific properties of membrane-linked enzymes analyzed from the viewpoints of modem enzymology. The level of sophistication in various areas of membrane research varied widely; the structures of cytochrome c and cytochrome b5 were known to atomic detail, while the majority of membrane-linked enzymes had not even been isolated. In the intervening eight years our knowledge of membrane-linked enzymes ex panded beyond the wildest expectations. The purpose of the second edition of The Enzymes of Biological Membranes is to record these developments. The first volume describes the physical and chemical techniques used in the analysis of the structure and dynamics of biological membranes. In the second volume the enzymes and met abolic systems that participate in the biosynthesis of cell and membrane components are discussed. The third and fourth volumes review recent developments in active transport, oxidative phosphorylation and photosynthesis.