Glycobiophysics

Author : Yoshiki Yamaguchi,Koichi Kato
Publisher : Springer
Page : 277 pages
File Size : 50,9 Mb
Release : 2018-11-27
Category : Science
ISBN : 9789811321580

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Glycobiophysics by Yoshiki Yamaguchi,Koichi Kato Pdf

This book presents state of the art biophysical approaches to issues in glycobiology that have cutting-edge applications. Despite the importance of glycosylation, the complexity, heterogeneity, and flexibility of the glycans have inhibited their study. Each chapter in this book explains very recent significant advances in biophysical approaches through the use of techniques such as NMR spectroscopy, mass spectrometry, single-molecule imaging, X-ray crystallography, high-speed atomic force microscopy, and computational simulation and their integrative application. Concrete examples are provided of the value of these techniques in addressing key problems in the field. In addition, significant functional glycobiological issues are considered. For example, glycolipids can form dynamic clusters on cell membranes and provide platforms for molecules involved in cell recognition and subsequent signal transduction. The detailed delineation of these molecular systems is discussed, revealing their structural complexity and ability to assemble transiently. This timely book will be of value for graduate students and postdocs interested in frontier topics in glycoscience and also for senior bio-researchers in academic and industrial fields.

Author : Masayoshi Nakasako
Publisher : Springer Nature
Page : 321 pages
File Size : 53,8 Mb
Release : 2021-10-25
Category : Science
ISBN : 9784431569190

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Hydration Structures of Proteins by Masayoshi Nakasako Pdf

This book describes hydration structures of proteins by combining experimental results with theoretical considerations. It is designed to introduce graduate students and researchers to microscopic views of the interactions between water and biological macromolecules and to provide them with an overview of the field. Topics on protein hydration from the past 25 years are examined, most of which involve crystallography, fluorescence measurements, and molecular dynamics simulations. In X-ray crystallography and molecular dynamics simulations, recent advances have accelerated the study of hydration structures over the entire surface of proteins. Experimentally, crystal structure analysis at cryogenic temperatures is advantageous in terms of visualizing the positions of hydration water molecules on the surfaces of proteins in their frozen-hydrated crystals. A set of massive data regarding hydration sites on protein surfaces provides an appropriate basis, enabling us to identify statistically significant trends in geometrical characteristics. Trajectories obtained from molecular dynamics simulations illustrate the motion of water molecules in the vicinity of protein surfaces at sufficiently high spatial and temporal resolution to study the influences of hydration on protein motion. Together with the results and implications of these studies, the physical principles of the measurement and simulation of protein hydration are briefly summarized at an undergraduate level. Further, the author presents recent results from statistical approaches to characterizing hydrogen-bond geometry in local hydration structures of proteins. The book equips readers to better understand the structures and modes of interaction at the interface between water and proteins. Referred to as “hydration structures”, they are the subject of much discussion, as they may help to answer the question of why water is indispensable for life at the molecular and atomic level.

Author : Tiffany Porta Siegel
Publisher : Royal Society of Chemistry
Page : 541 pages
File Size : 48,8 Mb
Release : 2021-11-23
Category : Science
ISBN : 9781839165207

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MALDI Mass Spectrometry Imaging by Tiffany Porta Siegel Pdf

This book gathers, in a single resource, knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging. It includes fundamentals in the MALDI ionisation process, different source geometries and capabilities, detection systems and the latest research and applications in the range of –omics area as well as other broader areas. Chapters will touch on dedicated sample preparation protocols specific for the class of compounds of interest, instrumentation used with strengths and current limitations, strategies for structural analysis and identification and applications. It will be a welcomed addition to the literature in this fast-moving field and provide a guide to new innovations and applications especially in metabolomics and proteomics. With contributions from leading experts, this book will be an authoritative guide to this method. Aimed at postgraduate and professional researchers, in academia and in the industrial market where it has direct application to clinical research. It will be a supporting volume for those just entering the field as well as experienced practitioners.

Author : Anonim
Publisher : Academic Press
Page : 562 pages
File Size : 48,6 Mb
Release : 2010-11-02
Category : Science
ISBN : 0123808553

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Advances in Carbohydrate Chemistry and Biochemistry by Anonim Pdf

Since its inception in 1945, this serial has provided critical and integrating articles written by research specialists that integrate industrial, analytical, and technological aspects of biochemistry, organic chemistry, and instrumentation methodology in the study of carbohydrates. The articles provide a definitive interpretation of the current status and future trends in carbohydrate chemistry and biochemistry. Features contributions from leading authorities and industry experts Informs and updates on all the latest developments in the field

Author : Alison E Ashcroft,Frank Sobott
Publisher : Royal Society of Chemistry
Page : 547 pages
File Size : 55,7 Mb
Release : 2021-11-11
Category : Science
ISBN : 9781839162893

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Ion Mobility-Mass Spectrometry by Alison E Ashcroft,Frank Sobott Pdf

Over the last decade, the use of ion mobility separation in combination with mass spectrometry analysis has developed significantly. This technique adds a unique extra dimension enabling the in-depth analysis of a wide range of complex samples in the areas of the chemical and biological sciences. Providing a comprehensive guide to the technique, each chapter is written by an internationally recognised expert and with numerous different commercial platforms to choose from, this book will help the end users understand the practicalities of using different instruments for different ion mobility purposes. The first section provides a detailed account of the fundamentals behind the technique and the current range of available instrumentation. The second section focusses on the wide range of applications that have benefitted from ion mobility – mass spectrometry and includes topics taken from current research in the pharmaceutical, metabolomics, glycomics, and structural molecular biology fields. The book is primarily aimed at researchers, appealing to practising chemists and biochemists, as well as those in the pharmaceutical and medical fields.

Author : Tiffany Siegel Porta
Publisher : Royal Society of Chemistry
Page : 541 pages
File Size : 41,7 Mb
Release : 2021-12-03
Category : Science
ISBN : 9781839162411

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MALDI Mass Spectrometry Imaging by Tiffany Siegel Porta Pdf

This book gathers knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging for postgraduate and professional researchers in academia and in industry where it has direct application to clinical research.

Author : Roy Jefferis,Koichi Kato,William R. (Bill) Strohl
Publisher : MDPI
Page : 440 pages
File Size : 50,6 Mb
Release : 2021-02-05
Category : Science
ISBN : 9783039438976

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Structure and Function of Antibodies by Roy Jefferis,Koichi Kato,William R. (Bill) Strohl Pdf

This book provides a detailed description of all kinds of therapeutic antibodies including IgGs, IgAs, IgEs, and IgMs, bispecific antibodies, chimeric antigen receptor antibodies, and antibody fragments. Details about how each of these antibodies interact with their ligands, the immune system, and their targets are provided. Additionally, this book delves into the details of antibody, Fc, and variable chain structures, and how subtle changes in structure, charge, flexibility, post-translational modification, and the ability to bind to natural antibody ligands can result in a significant impact on antibody activity and functionality. Finally, the book explains the critical quality attributes of modern therapeutic antibodies and how to ensure that antibodies entering development have the best possible chance of success.

Author : Ashok Pandey
Publisher : Springer Science & Business Media
Page : 774 pages
File Size : 51,8 Mb
Release : 2006-04-28
Category : Medical
ISBN : 0387292942

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Enzyme Technology by Ashok Pandey Pdf

Publisher Description

Author : Mark A. Williams,Tina Daviter
Publisher : Humana
Page : 0 pages
File Size : 42,8 Mb
Release : 2016-11-17
Category : Science
ISBN : 1493958739

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Protein-Ligand Interactions by Mark A. Williams,Tina Daviter Pdf

Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions – whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology.

Author : Giuseppe D'Alessio,James F. Riordan
Publisher : Academic Press
Page : 670 pages
File Size : 48,7 Mb
Release : 1997-02-11
Category : Science
ISBN : 0080540597

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Ribonucleases by Giuseppe D'Alessio,James F. Riordan Pdf

Pancreatic ribonuclease, the focus of highly productive scientific research for more than half a century and the only enzyme to be the basis of four Nobel prizes, has recently undergone a resurgence in popularity for the recognition of an extended ribonuclease superfamily with functions ranging from tumour growth and inhibition to self-recognition and neurotoxicity. This volume highlights the functional diversity of ribonucleases and reveals the emerging research opportunities provided by these enzymes. * Never before has discussion of the entire family of ribonucleases and related enzymes been covered in a single volume * Core chapters focus on the latest structures and functions of pancreatic-type ribonucleases * Structures and functions of intracellular ribonucleases and nondigestive members of the family are also covered * How ribonucleases continue to serve as excellent systems with which to uncover the secrets of protein chemistry is demonstrated

Author : Nico J. de Mol,Marcel J. E. Fischer
Publisher : Humana
Page : 286 pages
File Size : 49,5 Mb
Release : 2016-08-23
Category : Science
ISBN : 149396125X

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Surface Plasmon Resonance by Nico J. de Mol,Marcel J. E. Fischer Pdf

In this book, experts in the field present a wide variety of applications involving commercially available SPR instruments. The book fills a need for well-described, hands-on SPR experimental protocols, and it highlights the backgrounds of vital issues.

Author : Ajit Varki,Maarten J. Chrispeels
Publisher : CSHL Press
Page : 694 pages
File Size : 50,9 Mb
Release : 1999
Category : Medical
ISBN : 0879696818

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Essentials of Glycobiology by Ajit Varki,Maarten J. Chrispeels Pdf

Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.

Author : Andras Guttman,László Hajba
Publisher : Newnes
Page : 391 pages
File Size : 45,8 Mb
Release : 2021-12-04
Category : Science
ISBN : 9780080931357

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Capillary Gel Electrophoresis by Andras Guttman,László Hajba Pdf

Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Author : Jack G. Chirikjian,Takis S. Papas
Publisher : North-Holland
Page : 628 pages
File Size : 51,9 Mb
Release : 1981
Category : Science
ISBN : UCAL:B4530561

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Structural Analysis of Nucleic Acids by Jack G. Chirikjian,Takis S. Papas Pdf

Author : Naoyuk Taniguchi,Tamao Endo,Gerald W. Hart,Peter H. Seeberger,Chi-Huey Wong
Publisher : Springer
Page : 0 pages
File Size : 48,5 Mb
Release : 2014-11-14
Category : Science
ISBN : 4431548408

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Glycoscience: Biology and Medicine by Naoyuk Taniguchi,Tamao Endo,Gerald W. Hart,Peter H. Seeberger,Chi-Huey Wong Pdf

The aim of the book is to provide a succinct overview of the current status of glycoscience from both basic biological and medical points of view and to propose future directions, in order to facilitate further integrations of glycoscience with other fields in biological and medical studies. Glycans (carbohydrate oligomers) are the so-called “building blocks” of carbohydrates, nucleic acids, proteins and lipids and play major roles in many biological phenomena as well as in various pathophysiological processes. However, this area of glycoscience has been neglected from the research community because glycan structures are very complex and functionally diverse and as compared to proteins and nucleic acids simple tools for the amplification, sequencing and auto-synthesis of glycans are not available. Many scientists in other fields of research have now realized that glycosylation, i.e. the addition of glycans to a protein backbone, is the most abundant post translational modification reactions and is an important field of research and sometimes they require a glycobiology and/or glycochemistry approach to be used. It is still difficult, however, for non-expert researchers to use these techniques. This book provides numerous but simple overviews of current topics and protocols for the experiments. The book is aimed at university students and above, including non-experts in the field of glycoscience.