High Throughput Next Generation Sequencing

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High-Throughput Next Generation Sequencing

Young Min Kwon,Steven C. Ricke
High Throughput Next Generation Sequencing Book PDF

Author : Young Min Kwon,Steven C. Ricke
Publisher : Humana Press
Page : 0 pages
File Size : 47,5 Mb
Release : 2011-03-22
Category : Medical
ISBN : 1617790885

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High-Throughput Next Generation Sequencing by Young Min Kwon,Steven C. Ricke Pdf

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes. In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.

Next Generation Sequencing

Jerzy Kulski
Next Generation Sequencing Book PDF

Author : Jerzy Kulski
Publisher : BoD – Books on Demand
Page : 466 pages
File Size : 50,8 Mb
Release : 2016-01-14
Category : Medical
ISBN : 9789535122401

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Next Generation Sequencing by Jerzy Kulski Pdf

Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

Bioinformatics for High Throughput Sequencing

Naiara Rodríguez-Ezpeleta,Michael Hackenberg,Ana M. Aransay
Bioinformatics for High Throughput Sequencing Book PDF

Author : Naiara Rodríguez-Ezpeleta,Michael Hackenberg,Ana M. Aransay
Publisher : Springer Science & Business Media
Page : 255 pages
File Size : 51,7 Mb
Release : 2011-10-26
Category : Science
ISBN : 1461407826

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Bioinformatics for High Throughput Sequencing by Naiara Rodríguez-Ezpeleta,Michael Hackenberg,Ana M. Aransay Pdf

Next generation sequencing is revolutionizing molecular biology. Owing to this new technology it is now possible to carry out a panoply of experiments at an unprecedented low cost and high speed. These go from sequencing whole genomes, transcriptomes and small non-coding RNAs to description of methylated regions, identification protein – DNA interaction sites and detection of structural variation. The generation of gigabases of sequence information for each of this huge bandwidth of applications in just a few days makes the development of bioinformatics applications for next generation sequencing data analysis as urgent as challenging.

Next-Generation Sequencing Data Analysis

Xinkun Wang
Next Generation Sequencing Data Analysis Book PDF

Author : Xinkun Wang
Publisher : CRC Press
Page : 258 pages
File Size : 54,7 Mb
Release : 2016-04-06
Category : Mathematics
ISBN : 9781482217896

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Next-Generation Sequencing Data Analysis by Xinkun Wang Pdf

A Practical Guide to the Highly Dynamic Area of Massively Parallel SequencingThe development of genome and transcriptome sequencing technologies has led to a paradigm shift in life science research and disease diagnosis and prevention. Scientists are now able to see how human diseases and phenotypic changes are connected to DNA mutation, polymorphi

Next Generation Sequencing Technologies and Challenges in Sequence Assembly

Sara El-Metwally,Osama M. Ouda,Mohamed Helmy
Next Generation Sequencing Technologies and Challenges in Sequence Assembly Book PDF

Author : Sara El-Metwally,Osama M. Ouda,Mohamed Helmy
Publisher : Springer Science & Business
Page : 123 pages
File Size : 47,9 Mb
Release : 2014-04-19
Category : Science
ISBN : 9781493907151

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Next Generation Sequencing Technologies and Challenges in Sequence Assembly by Sara El-Metwally,Osama M. Ouda,Mohamed Helmy Pdf

The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.

New High Throughput Technologies for DNA Sequencing and Genomics

Keith R. Mitchelson
New High Throughput Technologies for DNA Sequencing and Genomics Book PDF

Author : Keith R. Mitchelson
Publisher : Elsevier
Page : 398 pages
File Size : 40,5 Mb
Release : 2011-09-22
Category : Science
ISBN : 0080471285

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New High Throughput Technologies for DNA Sequencing and Genomics by Keith R. Mitchelson Pdf

Since the independent invention of DNA sequencing by Sanger and by Gilbert 30 years ago, it has grown from a small scale technique capable of reading several kilobase-pair of sequence per day into today's multibillion dollar industry. This growth has spurred the development of new sequencing technologies that do not involve either electrophoresis or Sanger sequencing chemistries. Sequencing by Synthesis (SBS) involves multiple parallel micro-sequencing addition events occurring on a surface, where data from each round is detected by imaging. New High Throughput Technologies for DNA Sequencing and Genomics is the second volume in the Perspectives in Bioanalysis series, which looks at the electroanalytical chemistry of nucleic acids and proteins, development of electrochemical sensors and their application in biomedicine and in the new fields of genomics and proteomics. The authors have expertly formatted the information for a wide variety of readers, including new developments that will inspire students and young scientists to create new tools for science and medicine in the 21st century. Reviews of complementary developments in Sanger and SBS sequencing chemistries, capillary electrophoresis and microdevice integration, MS sequencing and applications set the framework for the book. * 'Hot Topic' with DNA sequencing continuing as a major research activity in many areas of life science and medicine. * Bringing together new developments in DNA sequencing technology * Reviewing issues relevant to the new applications used

Next Generation Sequencing and Applications

Preethi Kartan
Next Generation Sequencing and Applications Book PDF

Author : Preethi Kartan
Publisher : Delve Publishing
Page : 0 pages
File Size : 49,6 Mb
Release : 2017-11
Category : Bioinformatics
ISBN : 1773611100

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Next Generation Sequencing and Applications by Preethi Kartan Pdf

Billions of spots of tiny genetic code comprise the human genome. It was DNA sequencing technology that had revolutionized genomic research by decoding the valuable genetic information by giving the picture of an exact order of occurrence of nucleotides in a DNA. The inception of first-generation sequencing method, also called Sanger sequencing took place in 1975. The first major breakthrough of first-generation sequencing comes, when the 13 year log Human Genome Project (HGP) was completed in 2003 at a cost $3 million. With ever increasing demands of researchers and clinicians, complex genomic research require a depth of information which is however beyond the capacity of traditional DNA sequencing technologies. These research questions gaps are very well addressed by Next-generation sequencing (NGS) has filled that gap of cheaper as well as faster sequencing technology. It is just a decade old technology, but it has popularize the next-generation sequencing to high-throughput sequencing hat allow millions to trillions of observations to be made in parallel during a single instrument run. Since the introduction of these technologies, the number of applications and methods that influence the power of genome-scale sequencing has increased exponentially. Although in genome research NGS has mostly superseded conventional Sanger sequencing, it has not yet translated into routine clinical practice. The following chapter will highlight the concepts, technologies, and methods of next-generation sequencing to illustrate the breadth and depth of the applications and research areas that are driving progress in genomics.

Clinical Applications for Next-Generation Sequencing

Urszula Demkow,Rafal Ploski
Clinical Applications for Next Generation Sequencing Book PDF

Author : Urszula Demkow,Rafal Ploski
Publisher : Academic Press
Page : 334 pages
File Size : 45,6 Mb
Release : 2015-09-10
Category : Science
ISBN : 9780128018415

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Clinical Applications for Next-Generation Sequencing by Urszula Demkow,Rafal Ploski Pdf

Clinical Applications for Next Generation Sequencing provides readers with an outstanding postgraduate resource to learn about the translational use of NGS in clinical environments. Rooted in both medical genetics and clinical medicine, the book fills the gap between state-of-the-art technology and evidence-based practice, providing an educational opportunity for users to advance patient care by transferring NGS to the needs of real-world patients. The book builds an interface between genetic laboratory staff and clinical health workers to not only improve communication, but also strengthen cooperation. Users will find valuable tactics they can use to build a systematic framework for understanding the role of NGS testing in both common and rare diseases and conditions, from prenatal care, like chromosomal abnormalities, up to advanced age problems like dementia. Fills the gap between state-of-the-art technology and evidence-based practice Provides an educational opportunity which advances patient care through the transfer of NGS to real-world patient assessment Promotes a practical tool that clinicians can apply directly to patient care Includes a systematic framework for understanding the role of NGS testing in many common and rare diseases Presents evidence regarding the important role of NGS in current diagnostic strategies

Next-Generation Genome Sequencing

Michal Janitz
Next Generation Genome Sequencing Book PDF

Author : Michal Janitz
Publisher : John Wiley & Sons
Page : 281 pages
File Size : 52,9 Mb
Release : 2011-08-24
Category : Science
ISBN : 9783527644735

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Next-Generation Genome Sequencing by Michal Janitz Pdf

Written by leading experts from industry and academia, this first single comprehensive resource addresses recent developments in next generation DNA sequencing technology and their impact on genome research, drug discovery and health care. As such, it presents a detailed comparative analysis of commercially available platforms as well as insights into alternative, emerging sequencing techniques. In addition, the book not only covers the principles of DNA sequencing techniques but also social, ethical and commercial aspects, the concept of personalized medicine and a five-year perspective of DNA sequencing.

Next Generation Sequencing

Steven R. Head,Phillip Ordoukhanian,Daniel R. Salomon
Next Generation Sequencing Book PDF

Author : Steven R. Head,Phillip Ordoukhanian,Daniel R. Salomon
Publisher : Humana
Page : 0 pages
File Size : 45,6 Mb
Release : 2018-06-22
Category : Medical
ISBN : 1493985078

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Next Generation Sequencing by Steven R. Head,Phillip Ordoukhanian,Daniel R. Salomon Pdf

This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications, method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Next Generation Sequencing: Methods and Protocols aims to be useful and informative for further study into this vital field.

Childhood Acute Lymphoblastic Leukemia

Ajay Vora
Childhood Acute Lymphoblastic Leukemia Book PDF

Author : Ajay Vora
Publisher : Springer
Page : 342 pages
File Size : 51,8 Mb
Release : 2017-04-21
Category : Medical
ISBN : 9783319397085

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Childhood Acute Lymphoblastic Leukemia by Ajay Vora Pdf

This book provides a comprehensive and up-to-date review of all aspects of childhood Acute Lymphoblastic Leukemia, from basic biology to supportive care. It offers new insights into the genetic pre-disposition to the condition and discusses how response to early therapy and its basic biology are utilized to develop new prognostic stratification systems and target therapy. Readers will learn about current treatment and outcomes, such as immunotherapy and targeted therapy approaches. Supportive care and management of the condition in resource poor countries are also discussed in detail. This is an indispensable guide for research and laboratory scientists, pediatric hematologists as well as specialist nurses involved in the care of childhood leukemia.

Statistical Analysis of Next Generation Sequencing Data

Somnath Datta,Dan Nettleton
Statistical Analysis of Next Generation Sequencing Data Book PDF

Author : Somnath Datta,Dan Nettleton
Publisher : Springer
Page : 438 pages
File Size : 42,7 Mb
Release : 2014-07-03
Category : Medical
ISBN : 9783319072128

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Statistical Analysis of Next Generation Sequencing Data by Somnath Datta,Dan Nettleton Pdf

Next Generation Sequencing (NGS) is the latest high throughput technology to revolutionize genomic research. NGS generates massive genomic datasets that play a key role in the big data phenomenon that surrounds us today. To extract signals from high-dimensional NGS data and make valid statistical inferences and predictions, novel data analytic and statistical techniques are needed. This book contains 20 chapters written by prominent statisticians working with NGS data. The topics range from basic preprocessing and analysis with NGS data to more complex genomic applications such as copy number variation and isoform expression detection. Research statisticians who want to learn about this growing and exciting area will find this book useful. In addition, many chapters from this book could be included in graduate-level classes in statistical bioinformatics for training future biostatisticians who will be expected to deal with genomic data in basic biomedical research, genomic clinical trials and personalized medicine. About the editors: Somnath Datta is Professor and Vice Chair of Bioinformatics and Biostatistics at the University of Louisville. He is Fellow of the American Statistical Association, Fellow of the Institute of Mathematical Statistics and Elected Member of the International Statistical Institute. He has contributed to numerous research areas in Statistics, Biostatistics and Bioinformatics. Dan Nettleton is Professor and Laurence H. Baker Endowed Chair of Biological Statistics in the Department of Statistics at Iowa State University. He is Fellow of the American Statistical Association and has published research on a variety of topics in statistics, biology and bioinformatics.

Metagenomics

Wael N. Hozzein
Metagenomics Book PDF

Author : Wael N. Hozzein
Publisher : BoD – Books on Demand
Page : 164 pages
File Size : 48,5 Mb
Release : 2020-03-25
Category : Science
ISBN : 9781838800550

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Metagenomics by Wael N. Hozzein Pdf

This book is for the students starting their research projects in the field of metagenomics, for researchers interested in the new developments and applications in this field; and for teachers involved in teaching this subject. The book is divided into three sections as indicated from its title, namely; the basics of metagenomics, metagenomic analysis, and applications of metagenomics. It covers the basics of metagenomics from its history and background, to the analysis of metagenomic data as well as its recent applications in different fields. The book contains excellent texts at both the introductory and advanced levels, that describe the latest metagenomic approaches and applications, from sampling to data analysis for taxonomic, environmental, and medical studies. Finally, the publication of this book was an interesting journey for me and I hope the readers will enjoy reading it.

Algorithms for Next-Generation Sequencing Data

Mourad Elloumi
Algorithms for Next Generation Sequencing Data Book PDF

Author : Mourad Elloumi
Publisher : Springer
Page : 355 pages
File Size : 53,6 Mb
Release : 2017-09-18
Category : Computers
ISBN : 9783319598260

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Algorithms for Next-Generation Sequencing Data by Mourad Elloumi Pdf

The 14 contributed chapters in this book survey the most recent developments in high-performance algorithms for NGS data, offering fundamental insights and technical information specifically on indexing, compression and storage; error correction; alignment; and assembly. The book will be of value to researchers, practitioners and students engaged with bioinformatics, computer science, mathematics, statistics and life sciences.

Advancing Development of Synthetic Gene Regulators

Anandhakumar Chandran
Advancing Development of Synthetic Gene Regulators Book PDF

Author : Anandhakumar Chandran
Publisher : Springer
Page : 114 pages
File Size : 51,7 Mb
Release : 2017-09-19
Category : Science
ISBN : 9789811065477

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Advancing Development of Synthetic Gene Regulators by Anandhakumar Chandran Pdf

This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.