Instrumental Analysis Of Intrinsically Disordered Proteins

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Instrumental Analysis of Intrinsically Disordered Proteins

Vladimir Uversky,Sonia Longhi
Instrumental Analysis of Intrinsically Disordered Proteins Book PDF

Author : Vladimir Uversky,Sonia Longhi
Publisher : Wiley
Page : 760 pages
File Size : 51,6 Mb
Release : 2010-04-12
Category : Science
ISBN : 0470343419

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Instrumental Analysis of Intrinsically Disordered Proteins by Vladimir Uversky,Sonia Longhi Pdf

Instrumental techniques for analyzing intrinsically disordered proteins The recently recognized phenomenon of protein intrinsic disorder is gaining significant interest among researchers, especially as the number of proteins and protein domains that have been shown to be intrinsically disordered rapidly grows. The first reference to tackle this little-documented area, Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation provides researchers with a much-needed, comprehensive summary of recent achievements in the methods for structural characterization of intrinsically disordered proteins (IDPs). Chapters discuss: Assessment of IDPs in the living cell Spectroscopic techniques for the analysis of IDPs, including NMR and EPR spectroscopies, FTIR, circular dichroism, fluorescence spectroscopy, vibrational methods, and single-molecule analysis Single-molecule techniques applied to the study of IDPs Assessment of IDP size and shape Tools for the analysis of IDP conformational stability Mass spectrometry Approaches for expression and purification of IDPs With contributions from an international selection of leading researchers, Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation fills an important need in a rapidly growing field. It is required reading for biochemists, biophysicists, molecular biologists, geneticists, cell biologists, physiologists, and specialists in drug design and development, proteomics, and molecular medicine with an interest in proteins and peptides.

Instrumental Analysis of Intrinsically Disordered Proteins

Vladimir Uversky,Sonia Longhi
Instrumental Analysis of Intrinsically Disordered Proteins Book PDF

Author : Vladimir Uversky,Sonia Longhi
Publisher : John Wiley & Sons
Page : 792 pages
File Size : 51,5 Mb
Release : 2011-01-31
Category : Science
ISBN : 9780470602607

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Instrumental Analysis of Intrinsically Disordered Proteins by Vladimir Uversky,Sonia Longhi Pdf

Instrumental techniques for analyzing intrinsically disordered proteins The recently recognized phenomenon of protein intrinsic disorder is gaining significant interest among researchers, especially as the number of proteins and protein domains that have been shown to be intrinsically disordered rapidly grows. The first reference to tackle this little-documented area, Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation provides researchers with a much-needed, comprehensive summary of recent achievements in the methods for structural characterization of intrinsically disordered proteins (IDPs). Chapters discuss: Assessment of IDPs in the living cell Spectroscopic techniques for the analysis of IDPs, including NMR and EPR spectroscopies, FTIR, circular dichroism, fluorescence spectroscopy, vibrational methods, and single-molecule analysis Single-molecule techniques applied to the study of IDPs Assessment of IDP size and shape Tools for the analysis of IDP conformational stability Mass spectrometry Approaches for expression and purification of IDPs With contributions from an international selection of leading researchers, Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation fills an important need in a rapidly growing field. It is required reading for biochemists, biophysicists, molecular biologists, geneticists, cell biologists, physiologists, and specialists in drug design and development, proteomics, and molecular medicine with an interest in proteins and peptides.

Intrinsically Disordered Proteins

Anonim
Intrinsically Disordered Proteins Book PDF

Author : Anonim
Publisher : Academic Press
Page : 754 pages
File Size : 41,6 Mb
Release : 2018-11-21
Category : Science
ISBN : 9780128156506

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Intrinsically Disordered Proteins by Anonim Pdf

Intrinsically Disordered Proteins, Volume 611, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics of interest, including the Characterization of Structure-Function relationships in the intrinsically disordered protein complexin, Distances, distance distributions, and ensembles of IDPs from single-molecule FRET, Biophysical characterization of disordered protein liquid phases, The Use of Mass Spectrometry to Examine IDPs – Unique Insights and Caveats, Fluorescence Depolarization Kinetics to Study Conformational Preference, Structural Plasticity and Membrane Binding of Intrinsically Disordered Proteins, Characterizing the Function of Intrinsically Disordered Proteins in the Circadian Clock, and more. Breadth of experimental approaches and systems that will be covered The expertise of the contributors writing the articles

Intrinsically Disordered Protein Analysis

Vladimir N. Uversky,A. Keith Dunker
Intrinsically Disordered Protein Analysis Book PDF

Author : Vladimir N. Uversky,A. Keith Dunker
Publisher : Springer
Page : 454 pages
File Size : 44,6 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493944843

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Intrinsically Disordered Protein Analysis by Vladimir N. Uversky,A. Keith Dunker Pdf

Over the past decade, there has been an explosive development of research of intrinsically disordered proteins (IDPs), which are also known as unfolded proteins. Structural biologists now recognize that the functional diversity provided by disordered regions complements the functional repertoire of ordered protein regions. In Intrinsically Disordered Protein Analysis: Methods and Experimental Tools, expert researchers explore the high abundance of IDPs in various organisms, their unique structural features, numerous functions, and crucial associations with different diseases. Volume 2 includes sections on single molecule techniques, methods to assess protein size and shape, analyzing conformational behavior, mass-spectrometry, expression and purification of IDP’s. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Intrinsically Disordered Protein Analysis: Methods and Experimental Tools helps scientists further their investigations of these fascinating and dynamic molecules.

Intrinsically Disordered Proteins Studied by NMR Spectroscopy

Isabella C. Felli,Roberta Pierattelli
Intrinsically Disordered Proteins Studied by NMR Spectroscopy Book PDF

Author : Isabella C. Felli,Roberta Pierattelli
Publisher : Springer
Page : 421 pages
File Size : 46,7 Mb
Release : 2015-09-19
Category : Science
ISBN : 9783319201641

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Intrinsically Disordered Proteins Studied by NMR Spectroscopy by Isabella C. Felli,Roberta Pierattelli Pdf

This book discusses the paradigm-shifting phenomenon of intrinsically disordered proteins (IDPs) and hybrid proteins containing ordered domains and functional IDP regions (IDPRs). The properties of IDPs and IDPRs are highly complementary to those deriving from the presence of a unique and well-defined three-dimensional fold. Ignored for a long time in high-resolution studies of proteins, intrinsic protein disorder is now recognized as one of the key features for a large variety of cellular functions, where structural flexibility presents a functional advantage in terms of binding plasticity and promiscuity and this volume explores this exciting new research. Recent progress in the field has radically changed our perspective to study IDPs through NMR: increasingly complex IDPs can now be characterized, a wide range of observables can be determined reporting on the structural and dynamic properties, computational methods to describe the structure and dynamics are in continuous development and IDPs can be studied in environments as complex as whole cells. This volume communicates the new exciting possibilities offered by NMR and presents open questions to foster further developments. Intrinsically Disordered Proteins Studied by NMR Spectroscopy provides a snapshot to researchers entering the field as well as providing a current overview for more experienced scientists in related areas.

Structure and Intrinsic Disorder in Enzymology

Munishwar Nath Gupta,Vladimir N. Uversky
Structure and Intrinsic Disorder in Enzymology Book PDF

Author : Munishwar Nath Gupta,Vladimir N. Uversky
Publisher : Elsevier
Page : 529 pages
File Size : 42,6 Mb
Release : 2022-11-17
Category : Science
ISBN : 9780323995344

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Structure and Intrinsic Disorder in Enzymology by Munishwar Nath Gupta,Vladimir N. Uversky Pdf

Structure and Intrinsic Disorder in Enzymology offers a direct, yet comprehensive presentation of the fundamental concepts, characteristics and functions of intrinsically disordered enzymes, along with valuable notes and technical insights powering new research in this emerging field. Here, more than twenty international experts examine protein flexibility and cryo-enzymology, hierarchies of intrinsic disorder, methods for measurement of disorder in proteins, bioinformatics tools for predictions of structure, disorder and function, protein promiscuity, protein moonlighting, globular enzymes, intrinsic disorder and allosteric regulation, protein crowding, intrinsic disorder in post-translational, and much more. Chapters also review methods for study, as well as evolving technology to support new research across academic, industrial and pharmaceutical labs. Unifies the roles of intrinsic disorder and structure in the functioning of enzymes and proteins Examines a range of enzyme and protein characteristics, their relationship to intrinsic disorder, and methods for study Features chapter contributions from international leaders in the field

Intrinsically Disordered Protein Analysis

Vladimir N. Uversky,A. Keith Dunker
Intrinsically Disordered Protein Analysis Book PDF

Author : Vladimir N. Uversky,A. Keith Dunker
Publisher : Humana Press
Page : 511 pages
File Size : 47,6 Mb
Release : 2012-07-05
Category : Science
ISBN : 1617799289

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Intrinsically Disordered Protein Analysis by Vladimir N. Uversky,A. Keith Dunker Pdf

Over the past decade, there has been an explosive development of research of intrinsically disordered proteins (IDPs), which are also known as unfolded proteins. Structural biologists now recognize that the functional diversity provided by disordered regions complements the functional repertoire of ordered protein regions. In Intrinsically Disordered Protein Analysis :Methods and Experimental Tools, expert researchers explore the high abundance of IDPs in various organisms, their unique structural features, numerous functions, and crucial associations with different diseases. Volume 1 includes sections on assessing IDPs in the living cell,NMR based techniques, vibrational spectroscopy, and other spectroscopic techniques. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Intrinsically Disordered Protein Analysis: Methods and Experimental Tools helps scientists further their investigations of these fascinating and dynamic molecules.

Advances in Protein Molecular and Structural Biology Methods

Timir Tripathi,Vikash Kumar Dubey
Advances in Protein Molecular and Structural Biology Methods Book PDF

Author : Timir Tripathi,Vikash Kumar Dubey
Publisher : Academic Press
Page : 716 pages
File Size : 54,7 Mb
Release : 2022-01-14
Category : Science
ISBN : 9780323902656

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Advances in Protein Molecular and Structural Biology Methods by Timir Tripathi,Vikash Kumar Dubey Pdf

Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. Presents a thorough overview of the latest and emerging methods and technologies for protein study Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy Includes computational and machine learning methods Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins

Dancing Protein Clouds: Intrinsically Disordered Proteins in Health and Disease, Part B

Anonim
Dancing Protein Clouds Intrinsically Disordered Proteins in Health and Disease Part B Book PDF

Author : Anonim
Publisher : Academic Press
Page : 402 pages
File Size : 45,6 Mb
Release : 2020-08-19
Category : Science
ISBN : 9780128226049

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Dancing Protein Clouds: Intrinsically Disordered Proteins in Health and Disease, Part B by Anonim Pdf

Dancing Protein Clouds: Intrinsically Disordered Proteins in Health and Disease, Part B, represents a set of selected studies on a variety of research topics related to intrinsically disordered proteins. Topics in this update include structural and functional characterization of several important intrinsically disordered proteins, such as 14-3-3 proteins and their partners, as well as proteins from muscle sarcomere; representation of intrinsic disorder-related concept of protein structure-function continuum; discussion of the role of intrinsic disorder in phenotypic switching; consideration of the role of intrinsically disordered proteins in the pathogenesis of neurodegenerative diseases and cancer; discussion of the roles of intrinsic disorder in functional amyloids; demonstration of the usefulness of the analysis of translational diffusion of unfolded and intrinsically disordered proteins; consideration of various computational tools for evaluation of functions of intrinsically disordered regions; and discussion of the role of shear stress in the amyloid formation of intrinsically disordered regions in the brain. Provides some recent studies on the intrinsically disordered proteins and their functions, as well as on the involvement of intrinsically disordered proteins in pthogenesis of various diseases Contains numerous illustrative materials (color figures, diagrams, and tables) to help the readers to delve in the information provided Includes contributions from recognized experts in the field

Structural Biology in Drug Discovery

Jean-Paul Renaud
Structural Biology in Drug Discovery Book PDF

Author : Jean-Paul Renaud
Publisher : John Wiley & Sons
Page : 688 pages
File Size : 44,8 Mb
Release : 2020-01-27
Category : Medical
ISBN : 9781118900406

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Structural Biology in Drug Discovery by Jean-Paul Renaud Pdf

With the most comprehensive and up-to-date overview of structure-based drug discovery covering both experimental and computational approaches, Structural Biology in Drug Discovery: Methods, Techniques, and Practices describes principles, methods, applications, and emerging paradigms of structural biology as a tool for more efficient drug development. Coverage includes successful examples, academic and industry insights, novel concepts, and advances in a rapidly evolving field. The combined chapters, by authors writing from the frontlines of structural biology and drug discovery, give readers a valuable reference and resource that: Presents the benefits, limitations, and potentiality of major techniques in the field such as X-ray crystallography, NMR, neutron crystallography, cryo-EM, mass spectrometry and other biophysical techniques, and computational structural biology Includes detailed chapters on druggability, allostery, complementary use of thermodynamic and kinetic information, and powerful approaches such as structural chemogenomics and fragment-based drug design Emphasizes the need for the in-depth biophysical characterization of protein targets as well as of therapeutic proteins, and for a thorough quality assessment of experimental structures Illustrates advances in the field of established therapeutic targets like kinases, serine proteinases, GPCRs, and epigenetic proteins, and of more challenging ones like protein-protein interactions and intrinsically disordered proteins

Intrinsically Disordered Proteins

Nicola Salvi
Intrinsically Disordered Proteins Book PDF

Author : Nicola Salvi
Publisher : Academic Press
Page : 355 pages
File Size : 49,6 Mb
Release : 2019-06-14
Category : Science
ISBN : 9780128167328

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Intrinsically Disordered Proteins by Nicola Salvi Pdf

Intrinsically Disordered Proteins: Dynamics, Binding, and Function thoroughly examines and ties together the fundamental biochemical functions of intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs), including signaling, binding, and regulation, with the methodology for study and the associated pathways for drug design and therapeutic intervention. The role of new mechanistic, computational, and experimental approaches in IDP study are explored in depth, with methods for the characterization of IDP dynamics; models, simulations, and mechanisms of IDP and IDR binding; and biological and medical implications of IDP dynamics prominently featured. Written and edited by leading scientists in the field, this book explores groundbreaking areas such as ensemble descriptions of IDPs and IDRs, single-molecule studies of IDPs and IDRs, IDPs and IDRs in membraneless organelles, and molecular mechanisms of fibrillation of IDPs. Intrinsically Disordered Proteins provides students and researchers in biochemistry, molecular biology, and applied microbiology with a comprehensive and updated discussion of the complex dynamics of IDPs and IDRs. Provides in-depth discussion of fundamental IDP and IDR dynamics, function, and binding, with mechanistic insight to support new drug development Describes the role of new computational and experimental approaches in characterizing the binding of IDPs to their functional targets Features chapter contributions from international experts in IDP and IDR biochemical function and methods of study

Dancing protein clouds: Intrinsically disordered proteins in health and disease, Part A

Anonim
Dancing protein clouds Intrinsically disordered proteins in health and disease Part A Book PDF

Author : Anonim
Publisher : Academic Press
Page : 424 pages
File Size : 54,6 Mb
Release : 2019-09-11
Category : Science
ISBN : 9780128168523

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Dancing protein clouds: Intrinsically disordered proteins in health and disease, Part A by Anonim Pdf

"Dancing protein clouds: Intrinsically disordered proteins in the norm and pathology" represents a set of selected studies on a variety of research topics related to intrinsically disordered proteins. Topics in this update include structural and functional characterization of several important intrinsically disordered proteins, such as 14-3-3 proteins and their partners, as well as proteins from muscle sarcomere; representation of intrinsic disorder-related concept of protein structure-function continuum; discussion of the role of intrinsic disorder in phenotypic switching; consideration of the role of intrinsically disordered proteins in the pathogenesis of neurodegenerative diseases and cancer; discussion of the roles of intrinsic disorder in functional amyloids; demonstration of the usefulness of the analysis of translational diffusion of unfolded and intrinsically disordered proteins; consideration of various computational tools for evaluation of functions of intrinsically disordered regions; and discussion of the role of shear stress in the amyloid formation of intrinsically disordered regions in the brain. Provides some recent studies on the intrinsically disordered proteins and their functions, as well as on the involvement of intrinsically disordered proteins in pthogenesis of various diseases Contains numerous illustrative materials (color figures, diagrams, and tables) to help the readers to delve in the information provided Includes contributions from recognized experts in the field

Amino Acids, Peptides and Proteins Volume 44

Maxim Ryadnov,Ferenc Hudecz
Amino Acids Peptides and Proteins Volume 44 Book PDF

Author : Maxim Ryadnov,Ferenc Hudecz
Publisher : Royal Society of Chemistry
Page : 189 pages
File Size : 42,7 Mb
Release : 2020-12-08
Category : Science
ISBN : 9781788016896

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Amino Acids, Peptides and Proteins Volume 44 by Maxim Ryadnov,Ferenc Hudecz Pdf

Compiled by leading researchers, Amino Acids, Peptides and Proteins is a broad ranging title comprising comprehensive and critical reviews of significant developments at the biology and chemistry interface.

Intrinsically Disordered Proteins

Birthe B. Kragelund,Karen Skriver
Intrinsically Disordered Proteins Book PDF

Author : Birthe B. Kragelund,Karen Skriver
Publisher : Humana
Page : 951 pages
File Size : 54,9 Mb
Release : 2021-07-23
Category : Science
ISBN : 1071605267

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Intrinsically Disordered Proteins by Birthe B. Kragelund,Karen Skriver Pdf

The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Intrinsically Disordered Proteins: Methods and Protocols aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules. Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com. Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Functionally Relevant Macromolecular Interactions of Disordered Proteins

Istvan Simon
Functionally Relevant Macromolecular Interactions of Disordered Proteins Book PDF

Author : Istvan Simon
Publisher : MDPI
Page : 520 pages
File Size : 45,9 Mb
Release : 2021-01-20
Category : Science
ISBN : 9783039365210

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Functionally Relevant Macromolecular Interactions of Disordered Proteins by Istvan Simon Pdf

Disordered proteins are relatively recent newcomers in protein science. They were first described in detail by Wright and Dyson, in their J. Mol. Biol. paper in 1999. First, it was generally thought for more than a decade that disordered proteins or disordered parts of proteins have different amino acid compositions than folded proteins, and various prediction methods were developed based on this principle. These methods were suitable for distinguishing between the disordered (unstructured) and structured proteins known at that time. In addition, they could predict the site where a folded protein binds to the disordered part of a protein, shaping the latter into a well-defined 3D structure. Recently, however, evidence has emerged for a new type of disordered protein family whose members can undergo coupled folding and binding without the involvement of any folded proteins. Instead, they interact with each other, stabilizing their structure via “mutual synergistic folding” and, surprisingly, they exhibit the same residue composition as the folded protein. Increasingly more examples have been found where disordered proteins interact with non-protein macromolecules, adding to the already large variety of protein–protein interactions. There is also a very new phenomenon when proteins are involved in phase separation, which can represent a weak but functionally important macromolecular interaction. These phenomena are presented and discussed in the chapters of this book.