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Author : Hansjörg Hauser,Roland Wagner
Publisher : Walter de Gruyter
Page : 513 pages
File Size : 50,5 Mb
Release : 2011-07-13
Category : Science
ISBN : 9783110809282
„Hauser und Wagner haben die neuen Möglichkeiten der Mammalian Cell Biology sehr anregend dargestellt.“ Prof. Dr. Hans Fritz, Ludwig-Maximilians-Universität München
Author : David L. Hacker
Publisher : Humana
Page : 0 pages
File Size : 43,5 Mb
Release : 2024-08-17
Category : Science
ISBN : 1071638777
This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifying host genes involved in protein production. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols, Second Edition serves as an ideal guide for researchers investigatingprotein structure and function and accelerating the discovery of new therapeutic proteins.
Author : Gyun Min Lee,Helene Faustrup Kildegaard
Publisher : John Wiley & Sons
Page : 436 pages
File Size : 48,9 Mb
Release : 2020-01-13
Category : Science
ISBN : 9783527343348
Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.
Author : Lubiniecki
Publisher : Routledge
Page : 656 pages
File Size : 48,7 Mb
Release : 2018-05-02
Category : Medical
ISBN : 9781351435710
An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator
Author : Wei-Shu Hu
Publisher : Springer
Page : 179 pages
File Size : 40,9 Mb
Release : 2006-08-16
Category : Science
ISBN : 9783540340072
Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade.
Author : T.J.R. Harris
Publisher : Springer
Page : 250 pages
File Size : 52,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781461315650
There are very few parts of biology that remain free from the influence of Genetic Engineering developed in the early 1970s. Disciplines as wide apart as Brewing, Forensic Science and Population Genetics have all been affected in some way. The major impact, however, has been to create a new science of Biotechnology - a part of which is the production of proteins in a variety of cellular systems. Initially, bacterial systems such as E. coli were used but it soon became apparent that this prokaryotic host was not suitable for the preparation of more complicated proteins. In December 1988, a Symposium sponsored by the Biological Council organised by Dr Chris Hentschel and myself was held at the Middlesex in London to discuss alternative methods of Hospital Medical School protein production and to review some ofthe applications of the proteins so produced. The presentations at this meeting form the substance ofthis book. The theme is apparent from the first part where the expression of proteins and their domains in yeast is described and compared to other fungal and bacterial systems, such as Aspergillus and Bacillus subtilis. The successful use of recombinant yeast to produce hepatitis B surface antigen for vaccine purposes is particularly pertinent.
Author : Ralf Pörtner
Publisher : Springer Science & Business Media
Page : 1035 pages
File Size : 52,9 Mb
Release : 2007-04-05
Category : Science
ISBN : 9781588296603
The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The chapters are written by world-renowned experts and the volume’s five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.
Author : James L Hartley
Publisher : Humana Press
Page : 276 pages
File Size : 53,8 Mb
Release : 2011-10-12
Category : Science
ISBN : 1617793531
Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.
Author : Michael Butler
Publisher : Oxford University Press
Page : 245 pages
File Size : 49,6 Mb
Release : 1991
Category : Animal cell biotechnology.
ISBN : 019963209X
The development of mammalian cell biotechnology has led to an extensive range of compounds which can be routinely produced by cell culture. This book details the principles and practical techniques upon which this development is based.
Author : Mohamed Al-Rubeai
Publisher : Springer
Page : 766 pages
File Size : 55,7 Mb
Release : 2014-11-28
Category : Medical
ISBN : 9783319103204
Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.
Author : Weichang Zhou,Anne Kantardjieff
Publisher : Springer
Page : 262 pages
File Size : 44,5 Mb
Release : 2014-01-15
Category : Science
ISBN : 9783642540509
Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.
Author : Lubiniecki
Publisher : CRC Press
Page : 650 pages
File Size : 51,7 Mb
Release : 1990-09-14
Category : Medical
ISBN : 0824783271
An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator
Author : Anonim
Publisher : Academic Press
Page : 379 pages
File Size : 46,7 Mb
Release : 1994-10-18
Category : Science
ISBN : 0080859410
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. Describes both well-established and novel recombinant vector systems for expression of proteins Presents methods for efficient delivery of recombinant genes into differentiated cells, tissues, and whole animals Covers high-level and inducible systems, plus assays for protein expression Provides beginning and advanced investigators and students with the information they need to choose the optimal viral or plasmid system for their protein Practical, benchtop-style presentation works in lab and in the classroom
Author : Hansjörg Hauser,Roland Wagner
Publisher : Walter de Gruyter GmbH & Co KG
Page : 715 pages
File Size : 46,9 Mb
Release : 2014-11-10
Category : Science
ISBN : 9783110381429
This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.
Author : S.C. Makrides
Publisher : Gulf Professional Publishing
Page : 730 pages
File Size : 52,9 Mb
Release : 2003-10-24
Category : Science
ISBN : 0444513701
This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.