Mammalian Cell Viability

Author : Martin J. Stoddart
Publisher : Humana Press
Page : 0 pages
File Size : 45,7 Mb
Release : 2011-04-12
Category : Science
ISBN : 1617791075

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Mammalian Cell Viability by Martin J. Stoddart Pdf

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Author : TAPAN KUMAR. MUKHERJEE
Publisher : Unknown
Page : 0 pages
File Size : 46,8 Mb
Release : 2023-07
Category : Electronic
ISBN : 9811917302

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Practical Approach to Mamm by TAPAN KUMAR. MUKHERJEE Pdf

Author : Gyun Min Lee,Helene Faustrup Kildegaard
Publisher : John Wiley & Sons
Page : 436 pages
File Size : 46,6 Mb
Release : 2020-01-13
Category : Science
ISBN : 9783527343348

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Cell Culture Engineering by Gyun Min Lee,Helene Faustrup Kildegaard Pdf

Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.

Author : Joseph Feder
Publisher : Elsevier
Page : 170 pages
File Size : 50,8 Mb
Release : 2012-12-02
Category : Nature
ISBN : 9780323160780

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Large-Scale Mammalian Cell Culture by Joseph Feder Pdf

Large-Scale Mammalian Cell Culture is composed of papers presented as part of a symposium sponsored by the American Chemical Society Division of Microbial and Biochemical Technology at the 188th American Chemical Society National Meeting, held at Philadelphia, Pa., on Aug. 27, 1984. A rapid development of large-scale mammalian cell culture technology for the production of biologically important molecules becomes apparent. This book looks into this technology, its potential for commercial application, and the regulatory concerns posed by its use for the production of human therapeutics.

Author : Mohamed Al-Rubeai
Publisher : Springer
Page : 766 pages
File Size : 40,5 Mb
Release : 2014-11-28
Category : Medical
ISBN : 9783319103204

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Animal Cell Culture by Mohamed Al-Rubeai Pdf

​Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

Author : Jennie Mather
Publisher : Springer Science & Business Media
Page : 378 pages
File Size : 46,5 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461593614

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Mammalian Cell Culture by Jennie Mather Pdf

The advantages of obtaining a completely defined environment for the growth of cells in vitro were recognized very early in the history of cell culture (Lewis and Lewis, 1911). Continued interest in the nutritional requirements of cells in vitro and in providing an optimal environment for cells led to the development of the complex nutrient mixtures available today in many media (Waymouth, 1972; Ham, 1965). However, serum remained an essential component of medium for the growth of most cell types in culture. The question of what factor (or factors) in serum was essential for cell growth and survival remained unanswered for several decades. Initially, experiments were designed to purify the "active component" of serum for the growth of cells in culture. These experiments identified fetuin (Fisher et at., 1958) and nonsuppressible insulinlike activity (Temin et at., 1972) as important components of serum. However, the complexity of serum and the very low levels of active components in serum hindered progress in identi fying and isolating serum factors.

Author : Robert Pollack
Publisher : Cold Spring Harbor Laboratory Press
Page : 914 pages
File Size : 45,5 Mb
Release : 1975
Category : Nature
ISBN : UCAL:B4344420

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Readings in Mammalian Cell Culture by Robert Pollack Pdf

Author : Mohamed Al-Rubeai,A Nichol Emery
Publisher : CRC Press
Page : 323 pages
File Size : 48,8 Mb
Release : 2020-07-24
Category : Science
ISBN : 9781000148503

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Flow Cytometry Applications in Cell Culture by Mohamed Al-Rubeai,A Nichol Emery Pdf

This work present practical, biotechnological applications of flow cytometry techniques for the study of animal, plant and microbial cells, explaining methodologies for sample preparation, staining and analysis. It discusses cell variability in cell culture processes and shows how the quantitative analysis of heterogeneous populations aids in the biotechnological exploitation of cells.

Author : Ivan L. Cameron,Jack D. Thrasher
Publisher : Academic Press
Page : 433 pages
File Size : 42,9 Mb
Release : 2013-10-22
Category : Nature
ISBN : 9781483271385

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Cellular and Molecular Renewal in the Mammalian Body by Ivan L. Cameron,Jack D. Thrasher Pdf

Cellular and Molecular Renewal in the Mammalian Body concerns the dynamic nature of body constituents at the molecular, organelle, and cellular level of structural organization. Each chapter of this book deals with cellular and molecular renewal, proliferation, and loss, focusing on the body's major macromolecular classes, such as DNA, RNA, proteins, lipids, and carbohydrates. Other topics discussed include the subcellular renewal, DNA stability, regulation of cell proliferation, and changes in mitotic rates. The intracellular sites of RNA synthesis, proteins of the nucleus, and epithelial-mesenchymal interactions are also elaborated. This text likewise covers the brown adipose tissue, histochemistry of polysaccharides, and calcification. This publication is beneficial to students and investigators intending to acquire knowledge of the dynamic nature of body constituents and its influence to the analysis and experimentation of normal, disease, and repair processes within the body.

Author : Elsayed Ahmed Elsayed Ahmed
Publisher : Cuvillier Verlag
Page : 155 pages
File Size : 54,9 Mb
Release : 2005
Category : Electronic
ISBN : 9783865376343

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Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures by Elsayed Ahmed Elsayed Ahmed Pdf

Author : Lubiniecki
Publisher : Routledge
Page : 656 pages
File Size : 51,5 Mb
Release : 2018-05-02
Category : Medical
ISBN : 9781351435710

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Large-Scale Mammalian Cell Culture Technology by Lubiniecki Pdf

An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 43,6 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Thomas B. Shea,Peter C. W. Gaines
Publisher : Unknown
Page : 128 pages
File Size : 43,9 Mb
Release : 2018-11-27
Category : Electronic
ISBN : 1524977284

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Techniques in Mammalian Cell Culture by Thomas B. Shea,Peter C. W. Gaines Pdf

Author : Natalia Grigoryeva
Publisher : BoD – Books on Demand
Page : 464 pages
File Size : 43,8 Mb
Release : 2020-09
Category : Cytofluorometry
ISBN : 9781839680397

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Fluorescence Methods for Investigation of Living Cells and Microorganisms by Natalia Grigoryeva Pdf

Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

Author : OECD
Publisher : OECD Publishing
Page : 25 pages
File Size : 41,8 Mb
Release : 2015-07-28
Category : Electronic
ISBN : 9789264242241

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Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene by OECD Pdf

This TG includes two distinct in vitro mammalian gene mutation assays requiring two specific tk heterozygous cells lines: L5178Y tk+/-3.7.2C cells for the mouse lymphoma assay (MLA) and TK6 tk+/- cells for the TK6 assay.