Microfluidics For Single Cell Analysis

Author : Jin-Ming Lin
Publisher : Springer Nature
Page : 261 pages
File Size : 44,7 Mb
Release : 2019-08-28
Category : Science
ISBN : 9789813297296

Get Book

Microfluidics for Single-Cell Analysis by Jin-Ming Lin Pdf

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Author : Anonim
Publisher : Unknown
Page : 263 pages
File Size : 47,8 Mb
Release : 2019
Category : Cytology
ISBN : 9813297301

Get Book

Microfluidics for Single-cell Analysis by Anonim Pdf

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor's laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Author : Fan-Gang Tseng,Tuhin Subhra Santra
Publisher : MDPI
Page : 167 pages
File Size : 55,5 Mb
Release : 2018-10-04
Category : Electronic book
ISBN : 9783038421467

Get Book

Micro/Nanofluidic Devices for Single Cell Analysis by Fan-Gang Tseng,Tuhin Subhra Santra Pdf

This book is a printed edition of the Special Issue "Micro/Nanofluidic Devices for Single Cell Analysis" that was published in Micromachines

Author : Jin-Ming Lin
Publisher : Springer
Page : 429 pages
File Size : 43,7 Mb
Release : 2017-10-25
Category : Science
ISBN : 9789811053948

Get Book

Cell Analysis on Microfluidics by Jin-Ming Lin Pdf

This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Author : Nathalie Bontoux,Marie-Claude Potier
Publisher : Royal Society of Chemistry
Page : 332 pages
File Size : 49,5 Mb
Release : 2010-10-18
Category : Technology & Engineering
ISBN : 9781849732284

Get Book

Unravelling Single Cell Genomics by Nathalie Bontoux,Marie-Claude Potier Pdf

This unique introduction to the growing field of microfluidics applied to genomics provides an overview of the latest technologies and emphasizes its potential in answering important biological questions. Written by a physicist and a biologist, it offers a more comprehensive view than the previous literature. The book starts with key ideas in molecular biology, developmental biology and microtechnology before going on to cover the specifics of single cell analysis and microfluidic devices for single cell molecular analysis. Review chapters discuss the state-of-the art and will prove invaluable to all those planning to develop microdevices for molecular analysis of single cells. Methods allowing complete analysis of gene expression in the single cell are stressed - as opposed the more commonly used techniques that allow analysis of only a few genes at a time. As pioneers in the field, the authors understand how critical it is for a physicist to understand the biological issues and questions related to single cell analysis, as well for biologists to understand what microfluidics is all about. Aimed predominantly at graduate students, this book will also be of significant interest to scientists working in or affiliated with this field.

Author : Jian Chen,Yao Lu
Publisher : Academic Press
Page : 184 pages
File Size : 50,5 Mb
Release : 2021-10-26
Category : Science
ISBN : 9780323885591

Get Book

Biosensors for Single-Cell Analysis by Jian Chen,Yao Lu Pdf

Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers. Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization Features case studies that illustrate the application of biosensors for single-cell analysis Considers microfluidic approaches for each single-cell property discussed Explores future directions for single-cell analysis and biosensor technology

Author : Fan-Gang Tseng,Tuhin Subhra Santra
Publisher : MDPI
Page : 237 pages
File Size : 41,6 Mb
Release : 2018-10-01
Category : Electronic book
ISBN : 9783038421931

Get Book

Single Cell Analysis in Biotechnology and Systems Biology by Fan-Gang Tseng,Tuhin Subhra Santra Pdf

This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS

Author : Emmanuel Delamarche,Govind V. Kaigala
Publisher : John Wiley & Sons
Page : 450 pages
File Size : 47,6 Mb
Release : 2018-01-18
Category : Technology & Engineering
ISBN : 9783527696796

Get Book

Open-Space Microfluidics by Emmanuel Delamarche,Govind V. Kaigala Pdf

Summarizing the latest trends and the current state of this research field, this up-to-date book discusses in detail techniques to perform localized alterations on surfaces with great flexibility, including microfluidic probes, multifunctional nanopipettes and various surface patterning techniques, such as dip pen nanolithography. These techniques are also put in perspective in terms of applications and how they can be transformative of numerous (bio)chemical processes involving surfaces. The editors are from IBM Zurich, the pioneers and pacesetters in the field at the forefront of research in this new and rapidly expanding area.

Author : Samuel Kim
Publisher : Unknown
Page : 250 pages
File Size : 52,7 Mb
Release : 2009
Category : Electronic
ISBN : STANFORD:36105210267196

Get Book

Single-cell Analysis on Microfluidic Platforms by Samuel Kim Pdf

Author : Fan-Gang Tseng,Tuhin Subhra Santra
Publisher : Springer
Page : 414 pages
File Size : 53,7 Mb
Release : 2016-01-21
Category : Technology & Engineering
ISBN : 9783662491188

Get Book

Essentials of Single-Cell Analysis by Fan-Gang Tseng,Tuhin Subhra Santra Pdf

This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.

Author : Tuhin Subhra Santra,Fan-Gang Tseng
Publisher : MDPI
Page : 254 pages
File Size : 42,9 Mb
Release : 2021-06-02
Category : Science
ISBN : 9783036506289

Get Book

Single Cell Analysis by Tuhin Subhra Santra,Fan-Gang Tseng Pdf

Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.

Author : Paul C.H. Li,Angela Ruohao Wu
Publisher : Springer Nature
Page : 227 pages
File Size : 52,5 Mb
Release : 2023-07-10
Category : Science
ISBN : 9781071633236

Get Book

Single-Cell Assays by Paul C.H. Li,Angela Ruohao Wu Pdf

This detailed volume explores the use of single-cell assays in research for drug discovery, microfluidics, and more. The book delves into methodologies involving a variety of cell types and diseases, small molecules and biologics, as well as studies of the genome and transciptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Assays: Microfluidics, Genomics, and Drug Discovery serves to enable researchers to obtain a rapid overview in state-of-the-art microfluidic single-cell assays and an impression of what possibilities these assays offer to drug discovery.

Author : Anonim
Publisher : Academic Press
Page : 247 pages
File Size : 48,9 Mb
Release : 2018-08-27
Category : Science
ISBN : 9780128142837

Get Book

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells by Anonim Pdf

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration. Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Author : Dario Anselmetti
Publisher : John Wiley & Sons
Page : 284 pages
File Size : 40,9 Mb
Release : 2009-03-09
Category : Science
ISBN : 9783527626656

Get Book

Single Cell Analysis by Dario Anselmetti Pdf

The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.

Author : Paul C.H. Li
Publisher : CRC Press
Page : 420 pages
File Size : 45,7 Mb
Release : 2010-02-24
Category : Medical
ISBN : 9781439882177

Get Book

Fundamentals of Microfluidics and Lab on a Chip for Biological Analysis and Discovery by Paul C.H. Li Pdf

Lab-on-a-chip technology permits us to make many important discoveries that can only be observed at the microscale or the nanoscale. Using this technology, biological and biochemical analyses translate into greater sensitivity, more accurate results, and more valuable findings. Authored by one of the field's pioneering researchers, Fundamentals of