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Author : Steven E. Pfeiffer
Publisher : Unknown
Page : 208 pages
File Size : 41,5 Mb
Release : 1982
Category : Cell culture
ISBN : UOM:39015000785645
Author : Steven E. Pfeiffer
Publisher : Springer
Page : 256 pages
File Size : 49,5 Mb
Release : 1982-08-30
Category : Medical
ISBN : UOM:39015010062233
Author : Steven E Pfeiffer
Publisher : CRC Press
Page : 184 pages
File Size : 53,9 Mb
Release : 2022-01-12
Category : Electronic
ISBN : 0367260751
First published in 1983: This collection of papers, presented in two volumes, attempts to portray in a non-comprehensive manner some of the areas of current neurobiological research n which culture techniques have made, or now appear to have the potential to soon make, contributions to the questions being posed.
Author : Jane Bottenstein
Publisher : Springer Science & Business Media
Page : 392 pages
File Size : 54,8 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461324737
A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.
Author : George Lees,D. Beadle,Stanley B. Kater
Publisher : Unknown
Page : 264 pages
File Size : 52,9 Mb
Release : 1988
Category : Science
ISBN : UOM:39015013218162
Author : Laurie C. Doering
Publisher : Humana Press
Page : 400 pages
File Size : 48,7 Mb
Release : 2009-10-29
Category : Medical
ISBN : 1607612917
Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions’ tradition of exceptional quality and thorough coverage in tissue culture protocols for today’s neuroscience in order to inspire researchers in the field to further explore this imperative area of study.
Author : John Chad,H. V. Wheal
Publisher : Oxford University Press
Page : 316 pages
File Size : 52,6 Mb
Release : 1991
Category : Business & Economics
ISBN : 0199631077
Brings together a large number of techniques which are useful in exploring the functions of molecules, and the resolution of neurobiological processes at the cellular level.
Author : Michael Aschner,Lucio Costa
Publisher : Humana Press
Page : 302 pages
File Size : 46,8 Mb
Release : 2019-06-01
Category : Medical
ISBN : 1493992279
This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.
Author : Adalberto Merighi,Giorgio Carmignoto
Publisher : Springer Science & Business Media
Page : 307 pages
File Size : 55,8 Mb
Release : 2002-10-01
Category : Medical
ISBN : 9780387953861
There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.
Author : Michela Chiappalone,Valentina Pasquale,Monica Frega
Publisher : Springer
Page : 387 pages
File Size : 53,9 Mb
Release : 2019-05-09
Category : Medical
ISBN : 9783030111359
This book provides a comprehensive overview of the incredible advances achieved in the study of in vitro neuronal networks for use in basic and applied research. These cultures of dissociated neurons offer a perfect trade-off between complex experimental models and theoretical modeling approaches giving new opportunities for experimental design but also providing new challenges in data management and interpretation. Topics include culturing methodologies, neuroengineering techniques, stem cell derived neuronal networks, techniques for measuring network activity, and recent improvements in large-scale data analysis. The book ends with a series of case studies examining potential applications of these technologies.
Author : S. Fedoroff
Publisher : Elsevier
Page : 708 pages
File Size : 40,5 Mb
Release : 2012-12-02
Category : Nature
ISBN : 9780323143158
Cell, Tissue, and Organ Cultures in Neurobiology emerged from an international workshop held at the University of Saskatchewan in March 1977. This book reviews the uses of cell, tissue, and organ cultures in neurobiological research. It brings together an interdisciplinary perspective from morphology, biochemistry, pharmacology, endocrinology, embryology, and genetics. The book is organized into seven parts. Part I contains papers on the characteristics of differentiated cells. Part II presents studies on cell differentiation in primary cultures. Part III deals with studies on cell cultures and cell strains. Part IV focuses on phenotypic cell expression. Part V examines various cellular interactions. Part VI covers studies on nutrition while Part VII takes up applications of cell tissue and organ cultures in neurobiology. The book is directed toward tissue culturists concerned with the nervous system, as well as all neurobiologists, cell biologists, and embryologists interested in learning how neural cells and tissues behave in cultures and what has been learned about the nervous system using tissue culture methods, including the applicability of tissue cultures to the study of cell differentiation.
Author : Shohreh Amini,Martyn K. White
Publisher : Humana
Page : 205 pages
File Size : 40,8 Mb
Release : 2021-05-26
Category : Medical
ISBN : 1071614363
This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.
Author : Jane Bottenstein
Publisher : Unknown
Page : 408 pages
File Size : 48,7 Mb
Release : 1985-05-01
Category : Electronic
ISBN : 1461324742
Author : Sergey Fedoroff,Arleen Richardson
Publisher : Springer Science & Business Media
Page : 284 pages
File Size : 55,9 Mb
Release : 2013-04-17
Category : Medical
ISBN : 9781475725865
This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Canadian Council of Animal Care and the Medical Research Council of Canada. Sergey Fedoroff Arleen Richardson The second edition of Protocols for Neural Cell Culture adheres to the prin ciples enunciated in the first edition, but the content has been extensively revised and expanded. Two new chapters have been added to reflect the increased interest in the development and differentiation of the nervous system and in the reconstruction of its circuitry in tissue culture. One chapter deals with slice cul tures in which the organization of the nervous system is preserved. When slice cultures are combined with explant cultures, afferent and efferent projections can be reconstructed. The other chapter deals with aggregating neural cell cul tures, in which "minibrains" can form. Theses are small, uniformly sized spheres of nervous tissue, usually having nerve cells in the center and astrocytes, oligo dendrocytes, and microglia in the periphery. Such cultures can be used to study neutral cell interactions in an organized milieu and for qualitative as well as quantitative studies at biochemical and molecular levels.
Author : P. Michael Conn
Publisher : Unknown
Page : 454 pages
File Size : 45,8 Mb
Release : 1990
Category : Science
ISBN : UVA:X001810849
The ability to assess the response of neural cells is an important goal of research in the neurosciences. The means to measure responses in a chemically defined medium is a positive feature of the use of cell culture and is the major reason its use is so important in this discipline. Techniques for the purification of cells for culture, for large-scale isolation, for assessing differentiated function, and for tracing lineage are included. Many of the methods can easily be adapted to other systems. Key Features * Convenient benchtop format * Methods presented for easy adaptation to new systems * Comprehensive protocols included for * Preparation and maintenance of primary and continuous cultures * Cell purification * Bulk isolation * Studying pulsatility, growth on immobile carriers, rotation-mediated aggregation, microcultures, Maximow assembly cultures * Assessment of differentiated and functional aspects: endocrine modulation, morphological variants, receptors, intracellular trafficking * Clonal analysis and lineage analysis
