Optical Clearing Of Tissues And Blood

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Optical Clearing of Tissues and Blood

Valeriĭ Viktorovich Tuchin
Optical Clearing of Tissues and Blood Book PDF

Author : Valeriĭ Viktorovich Tuchin
Publisher : SPIE-International Society for Optical Engineering
Page : 276 pages
File Size : 49,7 Mb
Release : 2006
Category : Medical
ISBN : UOM:39015062485878

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Optical Clearing of Tissues and Blood by Valeriĭ Viktorovich Tuchin Pdf

This book describes an optical clearing method based on reversible reduction of tissue scattering due to refractive index matching of scatterers and ground matter. This technique, which has been of great interest for research and application in the last decade, is a promising technique for future developments in the fields of tissue imaging, spectroscopy, phototherapy, and laser surgery.

Handbook of Tissue Optical Clearing

Valery V. Tuchin,Dan Zhu,Elina A. Genina
Handbook of Tissue Optical Clearing Book PDF

Author : Valery V. Tuchin,Dan Zhu,Elina A. Genina
Publisher : CRC Press
Page : 682 pages
File Size : 40,9 Mb
Release : 2022-02-04
Category : Science
ISBN : 9781000466300

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Handbook of Tissue Optical Clearing by Valery V. Tuchin,Dan Zhu,Elina A. Genina Pdf

Biomedical photonics is currently one of the fastest growing fields, connecting research in physics, optics, and electrical engineering coupled with medical and biological applications. It allows for the structural and functional analysis of tissues and cells with resolution and contrast unattainable by any other methods. However, the major challenges of many biophotonics techniques are associated with the need to enhance imaging resolution even further to the sub-cellular level as well as translate them for in vivo studies. The tissue optical clearing method uses immersion of tissues into optical clearing agents (OCAs) that reduces the scattering of tissue and makes tissue more transparent and this method has been successfully used ever since. This book is a self-contained introduction to tissue optical clearing, including the basic principles and in vitro biological applications, from in vitro to in vivo tissue optical clearing methods, and combination of tissue optical clearing and various optical imaging for diagnosis. The chapters cover a wide range of issues related to the field of tissue optical clearing: mechanisms of tissue optical clearing in vitro and in vivo; traditional and innovative optical clearing agents; recent achievements in optical clearing of different tissues (including pathological tissues) and blood for optical imaging diagnosis and therapy. This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods. The book is addressed to biophysicist researchers, graduate students and postdocs of biomedical specialties, as well as biomedical engineers and physicians interested in the development and application of optical methods in medicine. Key features: The first collective reference to collate all known knowledge on this topic Edited by experts in the field with chapter contributions from subject area specialists Brings together the two main approaches in immersion optical clearing into one cohesive book

The Optical Clearing Method

Luís Manuel Couto Oliveira,Valery Victorovich Tuchin
The Optical Clearing Method Book PDF

Author : Luís Manuel Couto Oliveira,Valery Victorovich Tuchin
Publisher : Springer Nature
Page : 183 pages
File Size : 55,6 Mb
Release : 2019-11-27
Category : Science
ISBN : 9783030330552

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The Optical Clearing Method by Luís Manuel Couto Oliveira,Valery Victorovich Tuchin Pdf

This book describes the Optical Immersion Clearing method and its application to acquire information with importance for clinical practice and various fields of biomedical engineering. The method has proved to be a reliable means of increasing tissue transparency, allowing the investigator or surgeon to reach deeper tissue layers for improved imaging and laser surgery. This result is obtained by partial replacement of tissue water with an active optical clearing agent (OCA) that has a higher refractive index and is a better match for the refractive index of other tissue components. Natural tissue scattering is thereby reduced. An exponential increase in research using this method has occurred in recent years, and new applications have emerged, both in clinical practice and in some areas of biomedical engineering. Recent research has revealed that treating ex vivo tissues with solutions containing active OCAs in different concentrations produces experimental data to characterize drug delivery or to discriminate between normal and pathological tissues. The obtained drug diffusion properties are of interest for the pharmaceutical and organ preservation industry. Similar data can be estimated with particular interest for food preservation. The free water content evaluation is also of great interest since it facilitates the characterization of tissues to discriminate pathologies. An interesting new application that is presented in the book regards the creation of two optical windows in the ultraviolet spectral range through the application of the immersion method. These induced transparency windows open the possibility to diagnose and treat pathologies with ultraviolet light. This book presents photographs from the tissues we have studied and figures that represent the experimental setups used. Graphs and tables are also included to show the numerical results obtained in the sequential calculations performed.

Second Harmonic Generation Imaging

Francesco S. Pavone,Paul J. Campagnola
Second Harmonic Generation Imaging Book PDF

Author : Francesco S. Pavone,Paul J. Campagnola
Publisher : Taylor & Francis
Page : 465 pages
File Size : 53,8 Mb
Release : 2016-04-19
Category : Science
ISBN : 9781439849156

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Second Harmonic Generation Imaging by Francesco S. Pavone,Paul J. Campagnola Pdf

Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a

Optical-Thermal Response of Laser-Irradiated Tissue

Ashley J. Welch,Martin J.C. van Gemert
Optical Thermal Response of Laser Irradiated Tissue Book PDF

Author : Ashley J. Welch,Martin J.C. van Gemert
Publisher : Springer Science & Business Media
Page : 951 pages
File Size : 54,5 Mb
Release : 2011-01-15
Category : Science
ISBN : 9789048188314

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Optical-Thermal Response of Laser-Irradiated Tissue by Ashley J. Welch,Martin J.C. van Gemert Pdf

The second edition maintains the standard of excellence established in the first edition, while adjusting the content to reflect changes in tissue optics and medical applications since 1995. The material concerning light propagation now contains new chapters devoted to electromagnetic theory for coherent light. The material concerning thermal laser-tissue interactions contains a new chapter on pulse ablation of tissue. The medical applications section now includes several new chapters on Optical Coherent Tomography, acoustic imaging, molecular imaging, forensic optics and nerve stimulation. A detailed overview is provided of the optical and thermal response of tissue to laser irradiation along with diagnostic and therapeutic examples including fiber optics. Sufficient theory is included in the book so that it is suitable for a one or two semester graduate or for senior elective courses. Material covered includes (1) light propagation and diagnostic application; (2) the thermal response of tissue and therapeutic application; (3) denaturation; and (4) ablation. The theory and applications provide researchers with sufficient detail that this volume will become the primary reference for laser-tissue interactions and medical applications.

Tissue Optics

Valery Tuchin
Tissue Optics Book PDF

Author : Valery Tuchin
Publisher : Unknown
Page : 988 pages
File Size : 55,7 Mb
Release : 2015
Category : Diagnostic imaging
ISBN : 1628415169

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Tissue Optics by Valery Tuchin Pdf

This third edition of the biomedical optics classic Tissue Optics covers the continued intensive growth in tissue optics—in particular, the field of tissue diagnostics and imaging—that has occurred since 2007. As in the first two editions, Part I describes fundamentals and basic research, and Part II presents instrumentation and medical applications. However, for the reader’s convenience, this third edition has been reorganized into 14 chapters instead of 9. The chapters covering optical coherence tomography, digital holography and interferometry, controlling optical properties of tissues, nonlinear spectroscopy, and imaging have all been substantially updated. The book is intended for researchers, teachers, and graduate and undergraduate students specializing in the physics of living systems, biomedical optics and biophotonics, laser biophysics, and applications of lasers in biomedicine. It can also be used as a textbook for courses in medical physics, medical engineering, and medical biology.

Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues

Valery V. Tuchin
Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues Book PDF

Author : Valery V. Tuchin
Publisher : CRC Press
Page : 744 pages
File Size : 50,7 Mb
Release : 2008-12-22
Category : Science
ISBN : 1584889756

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Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues by Valery V. Tuchin Pdf

Although noninvasive, continuous monitoring of glucose concentration in blood and tissues is one of the most challenging areas in medicine, a wide range of optical techniques has recently been designed to help develop robust noninvasive methods for glucose sensing. For the first time in book form, the Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues analyzes trends in noninvasive optical glucose sensing and discusses its impact on tissue optical properties. This handbook presents methods that improve the accuracy in glucose prediction based on infrared absorption spectroscopy, recent studies on the influence of acute hyperglycemia on cerebral blood flow, and the correlation between diabetes and the thermo-optical response of human skin. It examines skin glucose monitoring by near-infrared spectroscopy (NIR), fluorescence-based glucose biosensors, and a photonic crystal contact lens sensor. The contributors also explore problems of polarimetric glucose sensing in transparent and turbid tissues as well as offer a high-resolution optical technique for noninvasive, continuous, and accurate blood glucose monitoring and glucose diffusion measurement. Written by world-renowned experts in biomedical optics and biophotonics, this book gives a complete, state-of-the-art treatise on the design and applications of noninvasive optical methods and instruments for glucose sensing.

Handbook of Optical Biomedical Diagnostics

Valeriĭ Viktorovich Tuchin
Handbook of Optical Biomedical Diagnostics Book PDF

Author : Valeriĭ Viktorovich Tuchin
Publisher : Unknown
Page : 688 pages
File Size : 47,7 Mb
Release : 2016
Category : Electronic books
ISBN : 1628419121

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Handbook of Optical Biomedical Diagnostics by Valeriĭ Viktorovich Tuchin Pdf

This text begins by describing the basic principles and diagnostic applications of optical techniques based on detecting and processing the scattering, fluorescence, FT IR, and Raman spectroscopic signals from various tissues, with an emphasis on blood, epithelial tissues, and human skin. The second half of the volume discusses specific imaging technologies, such as Doppler, laser speckle, optical coherence tomography (OCT), and fluorescence and photoacoustic imaging.

Tissue Optics

Valeriĭ Viktorovich Tuchin
Tissue Optics Book PDF

Author : Valeriĭ Viktorovich Tuchin
Publisher : CCH
Page : 882 pages
File Size : 40,7 Mb
Release : 2007
Category : Medical
ISBN : 0819464333

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Tissue Optics by Valeriĭ Viktorovich Tuchin Pdf

This second edition covers the intensive growth in tissue optics--in particular, the field of tissue diagnostics and imaging--that has occurred since 2000. As in the original edition, Part I describes fundamentals and basic research, and Part II presents instrumentation and medical applications. The extensive new material includes results on tissue optical property measurements, including polarized light interaction with turbid tissues; an overview of new polarization imaging and spectroscopy techniques, optical computed tomography (OCT) developments and applications; updates on controlling tissue optical properties, and the optothermal and optoacoustic interaction of light with tissues; and descriptions of fluorescence, nonlinear spectroscopies, and inelastic light scattering.

High Resolution Imaging in Microscopy and Ophthalmology

Josef F. Bille
High Resolution Imaging in Microscopy and Ophthalmology Book PDF

Author : Josef F. Bille
Publisher : Springer
Page : 407 pages
File Size : 46,5 Mb
Release : 2019-08-13
Category : Medical
ISBN : 9783030166380

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High Resolution Imaging in Microscopy and Ophthalmology by Josef F. Bille Pdf

This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

Confocal Microscopy

Stephen W. Paddock
Confocal Microscopy Book PDF

Author : Stephen W. Paddock
Publisher : Springer Science & Business Media
Page : 446 pages
File Size : 42,8 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597222

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Confocal Microscopy by Stephen W. Paddock Pdf

In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.

Advanced Optical Flow Cytometry

Valery V. Tuchin
Advanced Optical Flow Cytometry Book PDF

Author : Valery V. Tuchin
Publisher : John Wiley & Sons
Page : 698 pages
File Size : 46,7 Mb
Release : 2011-03-31
Category : Science
ISBN : 9783527634293

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Advanced Optical Flow Cytometry by Valery V. Tuchin Pdf

A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.

Advanced Biophotonics

Ruikang K. Wang,Valery V Tuchin
Advanced Biophotonics Book PDF

Author : Ruikang K. Wang,Valery V Tuchin
Publisher : Taylor & Francis
Page : 726 pages
File Size : 44,5 Mb
Release : 2016-04-19
Category : Science
ISBN : 9781439895825

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Advanced Biophotonics by Ruikang K. Wang,Valery V Tuchin Pdf

Despite a number of books on biophotonics imaging for medical diagnostics and therapy, the field still lacks a comprehensive imaging book that describes state-of-the-art biophotonics imaging approaches intensively developed in recent years. Addressing this shortfall, Advanced Biophotonics: Tissue Optical Sectioning presents contemporary methods and

Optical Imaging in Human Disease and Biological Research

Xunbin Wei,Bobo Gu
Optical Imaging in Human Disease and Biological Research Book PDF

Author : Xunbin Wei,Bobo Gu
Publisher : Springer Nature
Page : 309 pages
File Size : 54,7 Mb
Release : 2021-05-29
Category : Medical
ISBN : 9789811576270

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Optical Imaging in Human Disease and Biological Research by Xunbin Wei,Bobo Gu Pdf

The book introduces readers to the basic principle of optical imaging technologies. Focusing on human disease diagnostics using optical imaging methods, it provides essential information for researchers in various fields and discusses the latest trends in optical imaging. In recent decades, there has been a huge increase in imaging technologies and their applications in human diseases diagnostics, including magnetic resonance imaging, x-ray computed tomography, and nuclear tomographic imaging. This book promotes further developments to extend optical imaging to a wider range of disease diagnostics. It is a valuable resource for researchers and students in the field of biomedical optics, as well as for clinicians.

Neurohistology and Imaging Techniques

Radek Pelc,Wolfgang Walz,Ronald Doucette
Neurohistology and Imaging Techniques Book PDF

Author : Radek Pelc,Wolfgang Walz,Ronald Doucette
Publisher : Humana
Page : 471 pages
File Size : 48,6 Mb
Release : 2020-06-25
Category : Medical
ISBN : 1071604260

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Neurohistology and Imaging Techniques by Radek Pelc,Wolfgang Walz,Ronald Doucette Pdf

This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Neurohistology and Imaging Techniques is a valuable resource for both expert and novice users of major light microscopic imaging techniques, and those interested in exploring alternate imaging tools.