Patch Clamp Methods And Protocols

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Patch-Clamp Methods and Protocols

Peter Molnar,James J. Hickman
Patch Clamp Methods and Protocols Book PDF

Author : Peter Molnar,James J. Hickman
Publisher : Springer Science & Business Media
Page : 319 pages
File Size : 54,6 Mb
Release : 2007
Category : Medical
ISBN : 9781603270908

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Patch-Clamp Methods and Protocols by Peter Molnar,James J. Hickman Pdf

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.

Patch Clamp Electrophysiology

Mark Dallas,Damian Bell
Patch Clamp Electrophysiology Book PDF

Author : Mark Dallas,Damian Bell
Publisher : Humana
Page : 333 pages
File Size : 43,7 Mb
Release : 2021-11-13
Category : Medical
ISBN : 1071608207

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Patch Clamp Electrophysiology by Mark Dallas,Damian Bell Pdf

This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.

Patch-Clamp Methods and Protocols

Marzia Martina,Stefano Taverna
Patch Clamp Methods and Protocols Book PDF

Author : Marzia Martina,Stefano Taverna
Publisher : Humana
Page : 0 pages
File Size : 45,7 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493954148

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Patch-Clamp Methods and Protocols by Marzia Martina,Stefano Taverna Pdf

Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Patch-Cla mp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.

Patch Clamp Techniques

Yasunobu Okada
Patch Clamp Techniques Book PDF

Author : Yasunobu Okada
Publisher : Springer
Page : 0 pages
File Size : 43,8 Mb
Release : 2012-04-27
Category : Medical
ISBN : 4431539921

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Patch Clamp Techniques by Yasunobu Okada Pdf

Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.

Patch-Clamp Analysis

Wolfgang Walz,Alan A. Boulton,Glen B. Baker
Patch Clamp Analysis Book PDF

Author : Wolfgang Walz,Alan A. Boulton,Glen B. Baker
Publisher : Springer Science & Business Media
Page : 349 pages
File Size : 48,5 Mb
Release : 2008-02-06
Category : Medical
ISBN : 9781592592760

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Patch-Clamp Analysis by Wolfgang Walz,Alan A. Boulton,Glen B. Baker Pdf

Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.

Patch Clamping

Areles Molleman
Patch Clamping Book PDF

Author : Areles Molleman
Publisher : John Wiley & Sons
Page : 186 pages
File Size : 41,6 Mb
Release : 2003-06-13
Category : Science
ISBN : 9780470856512

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Patch Clamping by Areles Molleman Pdf

Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns

Patch-Clamp Methods and Protocols

Peter Molnar,James J. Hickman
Patch Clamp Methods and Protocols Book PDF

Author : Peter Molnar,James J. Hickman
Publisher : Humana Press
Page : 321 pages
File Size : 45,8 Mb
Release : 2007-09-19
Category : Science
ISBN : 1588296989

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Patch-Clamp Methods and Protocols by Peter Molnar,James J. Hickman Pdf

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. The volume includes an extensive theoretical treatise concerning single channel kinetic analysis.

Ion Channels

Nikita Gamper
Ion Channels Book PDF

Author : Nikita Gamper
Publisher : Humana Press
Page : 456 pages
File Size : 42,5 Mb
Release : 2016-05-01
Category : Science
ISBN : 1493960091

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Ion Channels by Nikita Gamper Pdf

This Methods in Molecular Biology book offers strategies and protocols for studying a large group of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Includes step-by-step protocols, materials lists, tips and more."

Patch-Clamp Applications and Protocols

Alan A. Boulton,Glen B. Baker,Wolfgang Walz
Patch Clamp Applications and Protocols Book PDF

Author : Alan A. Boulton,Glen B. Baker,Wolfgang Walz
Publisher : Humana Press
Page : 316 pages
File Size : 47,8 Mb
Release : 1995-05-01
Category : Medical
ISBN : 0896033112

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Patch-Clamp Applications and Protocols by Alan A. Boulton,Glen B. Baker,Wolfgang Walz Pdf

E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique.

Patch Clamp Technique

Fatima Shad Kaneez
Patch Clamp Technique Book PDF

Author : Fatima Shad Kaneez
Publisher : BoD – Books on Demand
Page : 372 pages
File Size : 52,5 Mb
Release : 2012-03-23
Category : Science
ISBN : 9789535104063

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Patch Clamp Technique by Fatima Shad Kaneez Pdf

This book is a stimulating and interesting addition to the collected works on Patch clamp technique. Patch Clamping is an electrophysiological technique, which measures the electric current generated by a living cell, due to the movement of ions through the protein channels present in the cell membrane. The technique was developed by two German scientists, Erwin Neher and Bert Sakmann, who received the Nobel Prize in 1991 in Physiology for this innovative work. Patch clamp technique is used for measuring drug effect against a series of diseases and to find out the mechanism of diseases in animals and plants. It is also most useful in finding out the structure function activities of compounds and drugs, and most leading pharmaceutical companies used this technique for their drugs before bringing them for clinical trial. This book deals with the understanding of endogenous mechanisms of cells and their receptors as well as advantages of using this technique. It covers the basic principles and preparation types and also deals with the latest developments in the traditional patch clamp technique. Some chapters in this book take the technique to a next level of modulation and novel approach. This book will be of good value for students of physiology, neuroscience, cell biology and biophysics.

Dynamic-Clamp

Alain Destexhe,Thierry Bal
Dynamic Clamp Book PDF

Author : Alain Destexhe,Thierry Bal
Publisher : Springer Science & Business Media
Page : 428 pages
File Size : 47,5 Mb
Release : 2009-03-11
Category : Medical
ISBN : 9780387892795

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Dynamic-Clamp by Alain Destexhe,Thierry Bal Pdf

Dynamic-clamp is a fascinating electrophysiology technique that consists of merging living neurons with computational models. The dynamic-clamp (also called “conductance injection”) allows experimentalists and theoreticians to challenge neurons (or any other type of cell) with complex conductance stimuli generated by a computer. The technique can be implemented from neural simulation environments and a variety of custom-made or commercial systems. The real-time interaction between the computer and cell also enables the design of recording paradigms with unprecedented accuracy via a computational model of the electrode. Dynamic-Clamp: From Principles to Applications contains contributions from leading researchers in the field, who investigate these paradigms at the cellular or network level, in vivo and in vitro, and in different brain regions and cardiac cells. Topics discussed include the addition of artificially-generated synaptic activity to neurons; adding, amplifying or neutralizing voltage-dependent conductances; creating hybrid networks with real and artificial cells; attaching simulated dendritic tree structures to the living cell; and connecting different neurons. This book will be of interest to experimental biophysicists, neurophysiologists, and cardiac physiologists, as well as theoreticians, engineers, and computational neuroscientists. Graduate and undergraduate students will also find up-to-date coverage of physiological problems and how they are investigated.

Cystic Fibrosis Methods and Protocols

William R. Skach
Cystic Fibrosis Methods and Protocols Book PDF

Author : William R. Skach
Publisher : Springer Science & Business Media
Page : 626 pages
File Size : 54,5 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592591879

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Cystic Fibrosis Methods and Protocols by William R. Skach Pdf

Since the cloning of the cystic fibrosis transmembrane conductance re- lator (CFTR) nearly a decade ago, cystic fibrosis (CF) research has witnessed a dramatic expansion into new scientific areas. Basic researchers, clinicians, and patients increasingly rely on fundamental techniques of genetics, molecular biology, electrophysiology, biochemistry, cell biology, microbiology, and immunology to understand the molecular basis of this complex disease. Research into the pathophysiology of CF has established numerous paradigms of ion channel dysfunction that extend from inflammation and infection in the airways of patients to basic mechanisms of protein processing and regulation in intracellular components. With these rapid advances has come an increasing need for research scientists to understand and utilize a growing array of basic laboratory tools. This volume of Methods in Molecular Medicine, Cystic Fibrosis Methods and Protocols satisfies that need by providing detailed protocols for the laboratory techniques used throughout CF research. From electrophysiology and cell biology, to animal models and gene therapy, the comprehensive set of methods covered here provide step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Contributions have been chosen to reflect the rich diversity of techniques and to provide a cohesive framework for understanding challenges that are currently at the forefront of CF research. It is hoped that this volume will serve as a valuable reference that will not only foster interdisciplinary investigations into current problems encountered in CF, but also facilitate the translation of new scientific discoveries into clinical solutions.

Connexin Methods and Protocols

Roberto Bruzzone,Christian Giaume
Connexin Methods and Protocols Book PDF

Author : Roberto Bruzzone,Christian Giaume
Publisher : Springer Science & Business Media
Page : 495 pages
File Size : 52,6 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590438

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Connexin Methods and Protocols by Roberto Bruzzone,Christian Giaume Pdf

Direct cell–cell communication is a common property of multicellular organisms that is achieved through membrane channels which are organized in gap junctions. The protein subunits of these intercellular channels, the connexins, form a multigene family that has been investigated in great detail in recent years. It has now become clear that, in different tissues, connexins speak several languages that control specific cellular functions. This progress has been made possible by the availability of new molecular tools and the improvement of basic techniques for the study of membrane channels, as well as by the use of genetic approaches to study protein function in vivo. More important, connexins have gained visibility because mutations in some connexin genes have been found to be linked to human genetic disorders. Connexin Methods and Protocols presents in detail a collection of te- niques currently used to study the cellular and molecular biology of connexins and their physiological properties. The field of gap junctions and connexin research has always been characterized by a multidisciplinary approach c- bining morphology, biochemistry, biophysics, and cellular and molecular biology. This book provides a series of cutting-edge protocols and includes a large spectrum of practical methods that are available to investigate the fu- tion of connexin channels. Connexin Methods and Protocols is divided into three main parts.

Single-Channel Recording

Bert Sakmann
Single Channel Recording Book PDF

Author : Bert Sakmann
Publisher : Springer Science & Business Media
Page : 506 pages
File Size : 43,8 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781461578581

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Single-Channel Recording by Bert Sakmann Pdf

Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol ogy.

Calcium Entry Channels in Non-Excitable Cells

Juliusz Ashot Kozak,James W. Putney, Jr.
Calcium Entry Channels in Non Excitable Cells Book PDF

Author : Juliusz Ashot Kozak,James W. Putney, Jr.
Publisher : CRC Press
Page : 343 pages
File Size : 43,9 Mb
Release : 2017-07-14
Category : Science
ISBN : 9781498752732

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Calcium Entry Channels in Non-Excitable Cells by Juliusz Ashot Kozak,James W. Putney, Jr. Pdf

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.