Patch Clamp Technique

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Patch Clamp Techniques

Yasunobu Okada
Patch Clamp Techniques Book PDF

Author : Yasunobu Okada
Publisher : Springer
Page : 0 pages
File Size : 42,9 Mb
Release : 2012-04-27
Category : Medical
ISBN : 4431539921

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Patch Clamp Techniques by Yasunobu Okada Pdf

Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.

Patch Clamp Electrophysiology

Mark Dallas,Damian Bell
Patch Clamp Electrophysiology Book PDF

Author : Mark Dallas,Damian Bell
Publisher : Humana
Page : 333 pages
File Size : 47,9 Mb
Release : 2021-11-13
Category : Medical
ISBN : 1071608207

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Patch Clamp Electrophysiology by Mark Dallas,Damian Bell Pdf

This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.

Patch Clamping

Areles Molleman
Patch Clamping Book PDF

Author : Areles Molleman
Publisher : John Wiley & Sons
Page : 186 pages
File Size : 47,5 Mb
Release : 2003-06-13
Category : Science
ISBN : 9780470856512

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Patch Clamping by Areles Molleman Pdf

Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns

Patch-Clamp Analysis

Wolfgang Walz,Alan A. Boulton,Glen B. Baker
Patch Clamp Analysis Book PDF

Author : Wolfgang Walz,Alan A. Boulton,Glen B. Baker
Publisher : Springer Science & Business Media
Page : 349 pages
File Size : 40,6 Mb
Release : 2008-02-06
Category : Medical
ISBN : 9781592592760

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Patch-Clamp Analysis by Wolfgang Walz,Alan A. Boulton,Glen B. Baker Pdf

Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.

Patch-Clamp Methods and Protocols

Peter Molnar,James J. Hickman
Patch Clamp Methods and Protocols Book PDF

Author : Peter Molnar,James J. Hickman
Publisher : Springer Science & Business Media
Page : 319 pages
File Size : 47,6 Mb
Release : 2007
Category : Medical
ISBN : 9781603270908

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Patch-Clamp Methods and Protocols by Peter Molnar,James J. Hickman Pdf

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.

Patch-Clamp Methods and Protocols

Marzia Martina,Stefano Taverna
Patch Clamp Methods and Protocols Book PDF

Author : Marzia Martina,Stefano Taverna
Publisher : Humana
Page : 0 pages
File Size : 53,5 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493954148

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Patch-Clamp Methods and Protocols by Marzia Martina,Stefano Taverna Pdf

Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Patch-Cla mp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.

Patch-Clamp Applications and Protocols

Alan A. Boulton,Glen B. Baker,Wolfgang Walz
Patch Clamp Applications and Protocols Book PDF

Author : Alan A. Boulton,Glen B. Baker,Wolfgang Walz
Publisher : Humana Press
Page : 316 pages
File Size : 52,5 Mb
Release : 1995-05-01
Category : Medical
ISBN : 0896033112

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Patch-Clamp Applications and Protocols by Alan A. Boulton,Glen B. Baker,Wolfgang Walz Pdf

E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique.

Gigaseal Formation in Patch Clamping

Majid Malboubi,Kyle Jiang
Gigaseal Formation in Patch Clamping Book PDF

Author : Majid Malboubi,Kyle Jiang
Publisher : Springer Science & Business Media
Page : 94 pages
File Size : 41,7 Mb
Release : 2013-07-24
Category : Technology & Engineering
ISBN : 9783642391286

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Gigaseal Formation in Patch Clamping by Majid Malboubi,Kyle Jiang Pdf

This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.

Single-Channel Recording

Bert Sakmann
Single Channel Recording Book PDF

Author : Bert Sakmann
Publisher : Springer Science & Business Media
Page : 506 pages
File Size : 42,9 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781461578581

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Single-Channel Recording by Bert Sakmann Pdf

Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol ogy.

Dynamic-Clamp

Alain Destexhe,Thierry Bal
Dynamic Clamp Book PDF

Author : Alain Destexhe,Thierry Bal
Publisher : Springer Science & Business Media
Page : 428 pages
File Size : 48,6 Mb
Release : 2009-03-11
Category : Medical
ISBN : 9780387892795

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Dynamic-Clamp by Alain Destexhe,Thierry Bal Pdf

Dynamic-clamp is a fascinating electrophysiology technique that consists of merging living neurons with computational models. The dynamic-clamp (also called “conductance injection”) allows experimentalists and theoreticians to challenge neurons (or any other type of cell) with complex conductance stimuli generated by a computer. The technique can be implemented from neural simulation environments and a variety of custom-made or commercial systems. The real-time interaction between the computer and cell also enables the design of recording paradigms with unprecedented accuracy via a computational model of the electrode. Dynamic-Clamp: From Principles to Applications contains contributions from leading researchers in the field, who investigate these paradigms at the cellular or network level, in vivo and in vitro, and in different brain regions and cardiac cells. Topics discussed include the addition of artificially-generated synaptic activity to neurons; adding, amplifying or neutralizing voltage-dependent conductances; creating hybrid networks with real and artificial cells; attaching simulated dendritic tree structures to the living cell; and connecting different neurons. This book will be of interest to experimental biophysicists, neurophysiologists, and cardiac physiologists, as well as theoreticians, engineers, and computational neuroscientists. Graduate and undergraduate students will also find up-to-date coverage of physiological problems and how they are investigated.

Basic Electrophysiological Methods

Ellen Covey,Matt Carter
Basic Electrophysiological Methods Book PDF

Author : Ellen Covey,Matt Carter
Publisher : Oxford University Press
Page : 256 pages
File Size : 52,5 Mb
Release : 2015-02-25
Category : Medical
ISBN : 9780199342549

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Basic Electrophysiological Methods by Ellen Covey,Matt Carter Pdf

Basic Electrophysiological Methods provides a concise and easy-to-read guide on a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with neuroscientific techniques. Intended for students, postdocs, and faculty with a basic neuroscience background, this text will not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Instead, each chapter provides a conscientious overview of the underlying theory -- a comprehensive description of equipment, materials, methods, data management, and analysis -- a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as strictly a DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.

Patch Clamp Technique

Fatima Shad Kaneez
Patch Clamp Technique Book PDF

Author : Fatima Shad Kaneez
Publisher : BoD – Books on Demand
Page : 372 pages
File Size : 40,5 Mb
Release : 2012-03-23
Category : Science
ISBN : 9789535104063

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Patch Clamp Technique by Fatima Shad Kaneez Pdf

This book is a stimulating and interesting addition to the collected works on Patch clamp technique. Patch Clamping is an electrophysiological technique, which measures the electric current generated by a living cell, due to the movement of ions through the protein channels present in the cell membrane. The technique was developed by two German scientists, Erwin Neher and Bert Sakmann, who received the Nobel Prize in 1991 in Physiology for this innovative work. Patch clamp technique is used for measuring drug effect against a series of diseases and to find out the mechanism of diseases in animals and plants. It is also most useful in finding out the structure function activities of compounds and drugs, and most leading pharmaceutical companies used this technique for their drugs before bringing them for clinical trial. This book deals with the understanding of endogenous mechanisms of cells and their receptors as well as advantages of using this technique. It covers the basic principles and preparation types and also deals with the latest developments in the traditional patch clamp technique. Some chapters in this book take the technique to a next level of modulation and novel approach. This book will be of good value for students of physiology, neuroscience, cell biology and biophysics.

Patch-Clamp Analysis

Wolfgang Walz
Patch Clamp Analysis Book PDF

Author : Wolfgang Walz
Publisher : Humana
Page : 0 pages
File Size : 54,7 Mb
Release : 2007-08-02
Category : Medical
ISBN : 1588297055

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Patch-Clamp Analysis by Wolfgang Walz Pdf

Continuing the research of the best-selling first edition, this second edition collects three more years of research in the ever-expanding study of the cell membrane. It covers the latest developments in the "traditional" patch techniques. This authoritative second edition updates the standard techniques while introducing three brand new, cutting-edge technical advances in the field. Thorough and timely, this edition is an invaluable resource.

Modern Tools of Biophysics

Thomas Jue
Modern Tools of Biophysics Book PDF

Author : Thomas Jue
Publisher : Springer
Page : 122 pages
File Size : 41,8 Mb
Release : 2017-05-07
Category : Medical
ISBN : 9781493967131

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Modern Tools of Biophysics by Thomas Jue Pdf

Handbook of Modern Biophysics brings current biophysics topics into focus, so that biology, medical, engineering, mathematics, and physical-science students or researchers can learn fun¬damental concepts and the application of new techniques in addressing biomedical challenges. Chapters will develop the conceptual framework of the physics formalism and illustrate the biomedical applica¬tions. With the addition of problem sets, guides to further study, and references, the interested reader can continue to independently explore the ideas presented.Volume 5: Modern Tools of BiophysicsEditor: Thomas Jue, PhDIn Modern Tools of Biophysics, a group of prominent professors have provided insights into the tools used in biophysics with respect to the following topics: Wave Theory of Image Formation in a Microscope: Basic Theory and Experiments Computer Simulations and Nonlinear Dynamics of Cardiac Action Potentials Myoglobin and Hemoglobin Contribution to the NIRS Signal in Muscle Anomalous Low Angle X-Ray Scattering of Membrane with Lanthanides Recording of Ionic Currents under Physiological Conditions—Action Potential-Clamping and “Onion-Peeling” Techniques Patch Clamp Technique and Applications About the EditorThomas Jue is a Professor in the Department of Biochemistry and Molecular Medicine at the University of California, Davis. He is an internationally recognized expert in developing and applying magnetic resonance techniques to study animal as well as human physiology in vivo and has published extensively in the field of magnetic resonance spectroscopy and imaging, near-infrared spectroscopy, bioenergetics, cardiovascular regulation, exercise, and marine biology. He served as a Chair of the Biophysics Graduate Group Program at UC Davis, where he started to develop scholarly approaches to educate graduate students with a balance of physical-science/mathematics formalism and biomedical perspective in order to promote interest at the interface of physical science, engineering, mathematics, biology, and medicine. He continues to develop the biophysics curriculum, and the Handbook of Modern Biophysics represents an aspect of that effort.

Manual of Research Techniques in Cardiovascular Medicine

Hossein Ardehali,Roberto Bolli,Douglas W. Losordo
Manual of Research Techniques in Cardiovascular Medicine Book PDF

Author : Hossein Ardehali,Roberto Bolli,Douglas W. Losordo
Publisher : John Wiley & Sons
Page : 1 pages
File Size : 45,6 Mb
Release : 2013-12-16
Category : Medical
ISBN : 9781118495131

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Manual of Research Techniques in Cardiovascular Medicine by Hossein Ardehali,Roberto Bolli,Douglas W. Losordo Pdf

While some research methods or techniques are applicable in several areas of medicine, research in cardiovascular diseases requires knowledge of an increasing array of procedures, techniques and measurements that are highly specialized and unique to this area of investigation. Edited by senior clinical investigators who are recognized leaders in cardiovascular medicine worldwide, this book provides readers with a comprehensive, practical “how-to-do-it” review of best-practice techniques for cardiovascular research.