Photoproteins In Bioanalysis

Author : Sylvia Daunert,Sapna K. Deo
Publisher : John Wiley & Sons
Page : 264 pages
File Size : 41,9 Mb
Release : 2006-10-06
Category : Science
ISBN : 3527310169

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Photoproteins in Bioanalysis by Sylvia Daunert,Sapna K. Deo Pdf

The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.

Author : Axel Griesbeck,Michael Oelgemöller,Francesco Ghetti
Publisher : CRC Press
Page : 1700 pages
File Size : 44,7 Mb
Release : 2019-04-05
Category : Medical
ISBN : 9781466561250

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CRC Handbook of Organic Photochemistry and Photobiology, Third Edition - Two Volume Set by Axel Griesbeck,Michael Oelgemöller,Francesco Ghetti Pdf

The only combined organic photochemistry and photobiology handbookAs spectroscopic, synthetic and biological tools become more and more sophisticated, photochemistry and photobiology are merging-making interdisciplinary research essential. Following in the footsteps of its bestselling predecessors, the CRC Handbook of Organic Photochemistry and Pho

Author : Gérald Thouand,Robert Marks
Publisher : Springer
Page : 203 pages
File Size : 48,7 Mb
Release : 2016-01-12
Category : Science
ISBN : 9783319274072

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Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 by Gérald Thouand,Robert Marks Pdf

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English. /div

Author : Shimshon Belkin,Man Bock Gu
Publisher : Springer
Page : 220 pages
File Size : 54,7 Mb
Release : 2010-07-13
Category : Science
ISBN : 9783642123627

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Whole Cell Sensing Systems I by Shimshon Belkin,Man Bock Gu Pdf

Table of Contents Reporter cells: - Yeast Based Sensors by Mifumi Shimomura-Shimizu and Isao Karube - Mammalian Cell-Based Sensor Systems by Pratik Banerjee, Briana Franz, and Arun K. Bhunia - Fluorescent and Bioluminescent Cell-Based Sensors: Strategies for Their Preservation by Amol Date, Patrizia Pasini, and Sylvia Daunert - Electrochemical Cell-Based Sensors by Eliora Z. Ron and Judith Rishpon - Microbial Cell Arrays by Tal Elad, Jin Hyung Lee, Man Bock Gu, and Shimshon Belkin Devices: - Surface Functionalization for Protein and Cell Patterning by Pascal Colpo , Ana Ruiz , Laura Ceriotti , and François Rossi - Fiber-Optic Based Cell Sensors by Evgeni Eltzov and Robert S. Marks - Electronic Interfacing with Living Cells by James T. Fleming - On-Chip Detection of Cellular Activity by R. Almog , R. Daniel , S. Vernick , A. Ron , H. Ben-Yoav, and Y. Shacham-Diamand

Author : Challa S. S. R. Kumar
Publisher : John Wiley & Sons
Page : 440 pages
File Size : 50,6 Mb
Release : 2007-02-12
Category : Science
ISBN : 3527313885

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Nanomaterials for Biosensors by Challa S. S. R. Kumar Pdf

This volume combines the chemistry and materials science of nanomaterials and biomolecules with their detection strategies, sensor physics and device engineering. In so doing, it covers the important types of nanomaterials for sensory applications, namely carbon nanotubes, fullerenes, fluorescent and biological molecules, nanorods, nanowires and nanoparticles, dendrimers, and nanostructured silicon. It also illustrates a wide range of sensing principles, including fluorescence, nanocantilever oscillators, electrochemical detection, antibody-antigen interactions, and magnetic detection.

Author : Toshio Yanagida,Yoshiharu Ishii
Publisher : John Wiley & Sons
Page : 347 pages
File Size : 49,6 Mb
Release : 2008-11-24
Category : Science
ISBN : 9783527312887

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Single Molecule Dynamics in Life Science by Toshio Yanagida,Yoshiharu Ishii Pdf

In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.

Author : Aldo Roda
Publisher : Royal Society of Chemistry
Page : 609 pages
File Size : 44,7 Mb
Release : 2011
Category : Medical
ISBN : 9781847558121

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Chemiluminescence and Bioluminescence by Aldo Roda Pdf

This comprehensive overview of chemiluminescence and bioluminescence covers historical developments, fundamental principles, recent advances, instrumentation, and applications.

Author : Hugo Barrera-Saldaña
Publisher : BoD – Books on Demand
Page : 272 pages
File Size : 46,7 Mb
Release : 2012-01-18
Category : Science
ISBN : 9789533077901

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Genetic Engineering by Hugo Barrera-Saldaña Pdf

Leading scientists from different countries around the world contributed valuable essays on the basic applications and safety, as well as the ethical and moral considerations, of the powerful genetic engineering tools now available for modifying the molecules, pathways, and phenotypes of species of agricultural, industrial and even medical importance. After three decades of perfecting such tools, we now see a refined technology, surprisingly unexpected applications, and matured guidelines to avoid unintentional damage to our and other species, as well as the environment, while trying to contribute to solve the biological, medical and technical challenges of society and industry. Chapters on thermo-stabilization of luciferase, engineering of the phenylpropanoid pathway in a species of high demand for the paper industry, more efficient regeneration of transgenic soybean, viral resistant plants, and a novel approach for rapidly screening properties of newly discovered animal growth hormones, illustrate the state-of-the-art science and technology of genetic engineering, but also serve to raise public awareness of the pros and cons that this young scientific discipline has to offer to mankind.

Author : Lawrence W. Miller
Publisher : John Wiley & Sons
Page : 204 pages
File Size : 50,6 Mb
Release : 2008-09-02
Category : Science
ISBN : 3527315667

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Probes and Tags to Study Biomolecular Function by Lawrence W. Miller Pdf

The only resource to systematically review current experimental methods, this handy reference enables researchers to select the best solution for their experimental problems. For each method covered, the book provides step-by-step protocols, illustrated by typical research applications. After an initial section on probing the lipid bilayer, the text moves on to discuss probing proteins -- including membrane proteins -- and nucleic acids. The first single publication to incorporate chemical markers, fluorescent probes and genetic tags allows a well-informed comparison of different solutions for the same problem in molecular analytics.

Author : Hirobumi Suzuki,Katsunori Ogoh
Publisher : BoD – Books on Demand
Page : 136 pages
File Size : 48,7 Mb
Release : 2021-12-15
Category : Technology & Engineering
ISBN : 9781839623851

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Bioluminescence by Hirobumi Suzuki,Katsunori Ogoh Pdf

This book contains seven chapters on bioluminescence techniques and organisms. On the technical side, the four chapters presented the fluorescent markers of proteins and nanocrystals, imidazopyrazine-type luciferin that emits light when bound to human serum albumin, firefly luciferin that emits near-infrared light, and imaging technique for visualization of promoter activity in fruiting body formation of cellular slime molds Dictyostelium. On the organismal side, the three chapters presented recommendations for the commercial use of fireflies in urban areas from the perspective of conservation biology, the origin of luciferin by predation in marine luminescent organisms, and the ecology and behavior of luminescent organisms from sea to land, which will be of interest to both professionals and students.

Author : Jeffrey J. Clare,Derek J. Trezise
Publisher : John Wiley & Sons
Page : 302 pages
File Size : 54,6 Mb
Release : 2006-08-21
Category : Science
ISBN : 9783527607938

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Expression and Analysis of Recombinant Ion Channels by Jeffrey J. Clare,Derek J. Trezise Pdf

Filling the gap created over the past five years, during which many new techniques have entered the market, this book is directed at both the new and the experienced ion channel researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. These latest developments are covered here for the first time, contributed by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function studies, from basic approaches for manipulating ion channel cDNAs to more specialized but powerful techniques. This is followed by reviews of strategies and methodologies available for expressing channels in mammalian cells and for their analysis by patch-clamp electrophysiology. Chapters 6 to 8 review the latest methodologies for ion channel drug discovery, including high throughput screening using fluorescence and luminescence, as well as automated planar array electrophysiology. The remaining two chapters focus on approaches for determining ion channel crystal structures and on computational approaches to understanding channel mechanisms at atomic resolution. Rather than provide detailed protocols, indicated by references in each chapter, the authors provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere. The result is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial workers alike.

Author : Maurice Goeldner,Richard Givens
Publisher : John Wiley & Sons
Page : 584 pages
File Size : 49,9 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527605545

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Dynamic Studies in Biology by Maurice Goeldner,Richard Givens Pdf

With contributions by more than 30 expert researchers, this handbook covers the whole spectrum from chemistry to cell biology and from theory to application. In so doing, it deals with a broad range of topics from the chemistry and biophysics of caged compounds to their application in time-resolved studies, comparing the properties of different caging groups. The authors describe in detail light-activation of proteins as well as nucleic acids, while a special section is devoted to multiphoton phototriggers. A must-have for every biochemist, biophysicist and molecular biologist developing and working with these novel methods.

Author : Anonim
Publisher : Academic Press
Page : 328 pages
File Size : 54,8 Mb
Release : 2010-11-26
Category : Science
ISBN : 0080951228

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Calcium in Living Cells by Anonim Pdf

Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases. Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging

Author : Anonim
Publisher : Unknown
Page : 290 pages
File Size : 40,9 Mb
Release : 2007
Category : Biosensors
ISBN : UOM:39015047296523

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Genetically Engineered and Optical Probes for Biomedical Applications by Anonim Pdf

Author : Arthur James Wells
Publisher : Unknown
Page : 1884 pages
File Size : 47,8 Mb
Release : 2006
Category : Bibliography, National
ISBN : UOM:39015066099196

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The British National Bibliography by Arthur James Wells Pdf