Protein Export And Membrane Biogenesis

Author : R.E. Dalbey
Publisher : Elsevier
Page : 276 pages
File Size : 53,9 Mb
Release : 1995-08-31
Category : Science
ISBN : 0080526098

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Protein Export and Membrane Biogenesis by R.E. Dalbey Pdf

The incentive for putting together Volume 4 of this series was to review the wealth of new information that has become available in prokaryotic organisms in protein export and membrane biogenesis. Just in the last several years, protein translocation has now been efficiently reconstituted using defined components and the mechanism by which proteins are moved across membrane bilayers is now being examined at a higher resolution. In addition, because of a new technical breakthrough using osmolytes, it is now possible to reconstitute a number of channel proteins, ATPase, receptors, and transporters. In many cases, it is possible to successfully predict the membrane topology of these types of proteins using both "hydrophobicity analysis" and the "positive inside" rule. In this volume, two chapters focus on protein translocation across membranes (Biochemical Analyses of Components Comprising the Protein Translocation Machinery of E. Coli; Protein Translocation Genetics), while several others on how proteins assemble into the ineer membrane of E. Coli (Membrane Protein Assembly; Membrane Insertion of Small Proteins: Evolutionary and Functional Aspects; Pigment-Protein Complex Assembly in Rhodobacter sphaeroides and Rhodobacter Capsulatus). Other sections review recent progress on transporters (Identification and Reconstitution of Anion Exchange Mechanisms in Bacteria; Helic Packing in the C-Terminal Half of Lactose Permease) and signal transduction (Mechanism of Transmembrane Signaling in Osmoregulation) as well as the assembly of prints into the outer membrane (Export and Assembly of Outer Membrane Proteins in E. coli). Although the emphasis of the book is on proteins, the role of phospholipids in controlling various cell surface processes is reviewed (Role of Phospholipids in coli Cell Function). I should point out the reason for the rapid progress in bacteria research is because of the possibility to apply biochemistry and genetics in this organism.

Author : W. Neupert,R. Lill
Publisher : Elsevier
Page : 340 pages
File Size : 49,6 Mb
Release : 1992-12-09
Category : Science
ISBN : 0080860826

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Membrane Biogenesis and Protein Targetting by W. Neupert,R. Lill Pdf

This book provides a comprehensive overview of recent developments in the fast moving field of protein transport across and into intracellular membranes. The soluble and membrane-bound components assisting in these processes are introduced, and their functions described. Besides a detailed analysis of protein translocation across the bacterial plasma membrane, the mechanisms of protein targeting within the eukaryotic cell are discussed with special focus on the transport of proteins to the endoplasmic reticulum, vacuoles, peroxisomes, mitochondria, and chloroplasts. Providing stimulating hypotheses and models, the book is well suited for advanced students, graduate students, and for newcomers to the field seeking a general, yet accurate introduction to present knowledge in membrane transport of proteins.

Author : Jos A.F. Op den Kamp
Publisher : Springer Science & Business Media
Page : 474 pages
File Size : 50,5 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783642731846

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Membrane Biogenesis by Jos A.F. Op den Kamp Pdf

Many individual aspects of the dynamics and assembly of biological membranes have been studied in great detail. Cell biological approaches, advanced genetics, biophysics and biochemistry have greatly contributed to an increase in our knowledge in this field.lt is obvious however, that the three major membrane constituents - lipids, proteins and carbohydrates- are studied, in most cases separately and that a coherent overview of the various aspects of membrane biogenesis is not readily available. The NATO Advanced Study Institute on "New Perspectives in the Dynamics of Assembly of Biomembranes" intended to provide such an overview: it was set up to teach students and specialists the achievements obtained in the various research areas and to try and integrate the numerous aspects of membrane assembly into a coherent framework. The articles in here reflect this. Statting with detailed contributions on phospholipid structure, dynamics, organization and biogenesis, an up to date overview of the basic, lipidic backbone of biomembranes is given. Extensive progress is made in the research on membrane protein biosynthesis. In particular the post- and co-translational modification processes of proteins, the mechanisms of protein translocation and the sorting mechanisms which are necessary to direct proteins to their final, intra - or extracellular destination have been characterized in detail. Modern genetic approaches were indispensable in this research area: gene cloning, hybrid protein construction, site directed mutagenesis and sequencing techniques elucidated many functional aspects of specific nucleic acid and amino acid sequences.

Author : Keith Verner,Jeanette Beers
Publisher : Unknown
Page : 198 pages
File Size : 48,7 Mb
Release : 1995
Category : Biological transport
ISBN : WISC:89057154874

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Intracellular Protein Transport and Membrane Biogenesis by Keith Verner,Jeanette Beers Pdf

This book is about membrane biogenesis and protein transport across biological membranes. Both prokaryotic and eukaryotic membrane transport systems are considered. The characteristics of targeting signals and membrane receptors on various intracellular organelles are described. Protein targeting to the endoplasmic reticulum and secretion via vesicular transport are discussed. In addition, recent developments in protein targeting and membrane transport to mitochondria, chloroplasts, peroxisomes and the nucleus are described. An introductory chapter on biological membranes is included as a review while a chapter on the transport of lipids to intracellular membranes precedes discussions on protein transport and membrane translocation.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 55,8 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Doron Rapaport,Johannes M. Herrmann
Publisher : Unknown
Page : 404 pages
File Size : 47,9 Mb
Release : 2013
Category : Cell membranes
ISBN : 1627034870

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Membrane Biogenesis by Doron Rapaport,Johannes M. Herrmann Pdf

Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques. In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.

Author : Maria Sandkvist,Eric Cascales,Peter J. Christie
Publisher : John Wiley & Sons
Page : 128 pages
File Size : 49,8 Mb
Release : 2020-07-02
Category : Science
ISBN : 9781683670285

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Protein Secretion in Bacteria by Maria Sandkvist,Eric Cascales,Peter J. Christie Pdf

Protein transport into and across membranes is a fundamental process in bacteria that touches upon and unites many areas of microbiology, including bacterial cell physiology, adhesion and motility, nutrient scavenging, intrabacterial signaling and social behavior, toxin deployment, interbacterial antagonism and collaboration, host invasion and disruption, and immune evasion. A broad repertoire of mechanisms and macromolecular machines are required to deliver protein substrates across bacterial cell membranes for intended effects. Some machines are common to most, if not all bacteria, whereas others are specific to Gram-negative or Gram-positive species or species with unique cell envelope properties such as members of Actinobacteria and Spirochetes. Protein Secretion in Bacteria, authored and edited by an international team of experts, draws together the many distinct functions and mechanisms involved in protein translocation in one concise tome. This comprehensive book presents updated information on all aspects of bacterial protein secretion encompassing: Individual secretory systems–Sec, Tat, and T1SS through the newly discovered T9SS Mechanisms, structures, and functions of bacterial secretion systems Lipoprotein sorting pathways, outer membrane vesicles, and the sortase system Structures and roles of surface organelles, including flagella, pili, and curli Emerging technologies and translational implications Protein Secretion in Bacteria serves as both an introductory guide for students and postdocs and a ready reference for seasoned researchers whose work touches on protein export and secretion. This volume synthesizes the diversity of mechanisms of bacterial secretion across the microbial world into a digestible resource to stimulate new research, inspire continued identification and characterization of novel systems, and bring about new ways to manipulate these systems for biotechnological, preventative, and therapeutic applications.

Author : A.H. Maddy,J. Robin Harris
Publisher : Springer Science & Business Media
Page : 424 pages
File Size : 42,6 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461524014

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Membrane Biogenesis by A.H. Maddy,J. Robin Harris Pdf

Scientific reviews are now of two complementary types: short, very up-to-date articles, as are found in the Trends series, and the more traditional longer re views, which are more comprehensive but take longer to publish. The Subcellu lar Biochemistry series belongs to the latter category where a number of reviews on a broad topic are collected together in the one volume. It has been the aim of this volume to summarize the present state of knowledge of membrane assembly. It is appreciated that some relevant topics have not been included, and an editor's selection is restricted by the many calls on potential authors who are unable to meet all requests made to them. The absence of a discussion of the roles of lipids is," however, a reflection of the fact that a recent volume in this series, edited by Dr. H. 1. Hilderson, has been devoted to this subject (Vol. 16, Intracellular Transfer of Lipid Molecules), and readers are recommended to this source.

Author : Philip Yeagle
Publisher : Unknown
Page : 376 pages
File Size : 48,9 Mb
Release : 1993
Category : Medical
ISBN : UOM:39015026979164

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The Membranes of Cells by Philip Yeagle Pdf

In this new edition of The Membranes of Cells, all of the chapters have been updated, some have been completely rewritten, and a new chapter on receptors has been added. The book has been designed to provide both the student and researcher with a synthesis of information from a number of scientific disciplines to create a comprehensive view of the structure and function of the membranes of cells. The topics are treated in sufficient depth to provide an entry point to the more detailed literature needed by the researcher. Key Features * Introduces biologists to membrane structure and physical chemistry * Introduces biophysicists to biological membrane function * Provides a comprehensive view of cell membranes to students, either as a necessary background for other specialized disciplines or as an entry into the field of biological membrane research * Clarifies ambiguities in the field

Author : Martin Chalfie,Steven R. Kain
Publisher : John Wiley & Sons
Page : 486 pages
File Size : 43,6 Mb
Release : 2005-11-18
Category : Science
ISBN : 9780471739487

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Green Fluorescent Protein by Martin Chalfie,Steven R. Kain Pdf

Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.

Author : Chris Mullins
Publisher : Springer Science & Business Media
Page : 191 pages
File Size : 48,7 Mb
Release : 2007-03-06
Category : Medical
ISBN : 9780387268675

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The Biogenesis of Cellular Organelles by Chris Mullins Pdf

The Biogenesis of Cellular Organelles represents a comprehensive summary of recent advances in the study of the biogenesis and functional dynamics of the major organelles operating in the eukaryotic cell. This book begins by placing the study of organelle biogenesis in a historical perspective by describing past scientific strategies, theories, and findings and relating these foundations to current investigations. Reviews of protein and lipid mediators important for organelle biogenesis are then presented, and are followed by summaries focused on the endoplasmic reticulum, Golgi, lysosome, nucleus, mitochondria, and peroxisome.

Author : S. Damjanovich
Publisher : CRC Press
Page : 673 pages
File Size : 41,9 Mb
Release : 2018-01-18
Category : Science
ISBN : 9781351091572

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Mobility and Proximity in Biological Membranes by S. Damjanovich Pdf

Cell surface membranes have long been characterized as two-dimensional fluids whose mobile components are randomized by diffusion in the plane of the membrane bilayer. Recent research has indicated that cell surface membranes are highly organized and ordered and that important functional units of membranes appear as arrays of interacting molecules rather than as single, freely diffusing molecules. Mobility and Proximity in Biological Membranes provides an overview of the results obtained from biophysical methods for probing the organization of cell surface membranes. These results are presented in the context of detailed treatments of the theory and the technical demands of each of the methods. The book describes a versatile and easily applied mode for investigating molecular proximities in plasma membranes in a flow cytometer. Its analysis of lipid fluidity and viscosity of membranes and the rotational mobility of proteins offers intimate insight into the physical chemistry of biological membranes. The electrophysiology of lymphocytes is presented with focus on its importance in different diseases. New techniques are described, and new data, new possibilities, and future trends are presented by world experts. This book's chapters can serve both as guides to the existing literature and as starting points for new experiments and approaches associated with problems in membrane function.

Author : Mary Luckey
Publisher : Cambridge University Press
Page : 427 pages
File Size : 48,7 Mb
Release : 2014-02-24
Category : Science
ISBN : 9781107729339

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Membrane Structural Biology by Mary Luckey Pdf

This textbook provides a strong foundation and a clear overview for students of membrane biology and an invaluable synthesis of cutting-edge research for working scientists. The text retains its clear and engaging style, providing a solid background in membrane biochemistry, while also incorporating the approaches of biophysics, genetics and cell biology to investigations of membrane structure, function and biogenesis to provide a unique overview of this fast-moving field. A wealth of new high resolution structures of membrane proteins are presented, including the Na/K pump and a receptor-G protein complex, offering exciting insights into how they function. All key tools of current membrane research are described, including detergents and model systems, bioinformatics, protein-folding methodology, crystallography and diffraction, and molecular modeling. This comprehensive and up-to-date text, emphasising the correlations between membrane research and human health, provides a solid foundation for all those working in this field.

Author : Sophie Bleves,Romé Voulhoux,Bérengère Ize,Thibault Géry Sana,Denise Monack
Publisher : Frontiers Media SA
Page : 198 pages
File Size : 52,9 Mb
Release : 2020-02-27
Category : Electronic
ISBN : 9782889635160

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Protein Export and Secretion Among Bacterial Pathogens by Sophie Bleves,Romé Voulhoux,Bérengère Ize,Thibault Géry Sana,Denise Monack Pdf

Author : S.S. Rothman
Publisher : Elsevier
Page : 305 pages
File Size : 51,7 Mb
Release : 1995-06-28
Category : Science
ISBN : 0080536131

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Membrane Protein Transport by S.S. Rothman Pdf

This is the first volume in a series on membrane protein transfer. Membrane protein transport underlies the topological disposition of many proteins within cells and it is this disposition that allows for the co-ordination of the central cellular processes, such as metabolism.