Rapid Freezing Freeze Fracture And Deep Etching

Author : Nicholas J. Severs,David M. Shotton
Publisher : Wiley-Liss
Page : 392 pages
File Size : 41,5 Mb
Release : 1995-08-11
Category : Medical
ISBN : UOM:39015037496695

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Rapid Freezing, Freeze Fracture, and Deep Etching by Nicholas J. Severs,David M. Shotton Pdf

The techniques of freeze fracture and deep etching—techniques for the examination of the ultrastructure of rapidly frozen biological specimens by transmission electron microscopy—have profoundly influenced our understanding of the functional organization of the cell. Rapid Freezing, Freeze Fracture, and Deep Etching guides the reader through the principles of these techniques and gives detailed examples of their application in biomedical research. The book is organized into three sections. The first covers the fundamentals of freeze fracture and deep etching, with a discussion of basic principles for those new to the field, and an entire chapter on the equipment currently available for freeze fracture. Advanced techniques are presented next, with each chapter devoted to an in-depth treatment of a specific topic, including the advantages and limitations of each technique. The final chapters offer a series of applications selected to illustrate the wide-ranging and enduring impact of rapid freezing, freeze fracture, and deep etching on the field of biomedical research. With over 150 illustrations and containing contributions by leading experts instrumental in the development of these techniques, Rapid Freezing, Freeze Fracture, and Deep Etching offers authoritative coverage of such novel topics as: Hyperbaric freezing Ultrarapid freezing techniques in the study of dynamic cellular processes Cytochemical methods utilizing lectins and antibodies for labeling specific cellcomponents Fracturing and shadowing under ultra-low temperature and ultra-high vacuum conditions Freeze fracture and deep etching in neurobiology Rapid Freezing, Freeze Fracture, and Deep Etching will appeal to both newcomers to the field and experienced researchers and will be essential reading for cell biologists and anatomists, as well as any researcher who uses electron microscopy.

Author : Arvid B. Maunsbach,Björn A. Afzelius
Publisher : Elsevier
Page : 548 pages
File Size : 45,9 Mb
Release : 1998-11-03
Category : Science
ISBN : 0080528090

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Biomedical Electron Microscopy by Arvid B. Maunsbach,Björn A. Afzelius Pdf

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Authored by the key leaders in the biological electron microscopy field Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines Introduces students on how to read and interpret electron micrographs

Author : Andrew I. Spielman,Joseph G. Brand
Publisher : CRC Press
Page : 468 pages
File Size : 47,9 Mb
Release : 1995-07-12
Category : Science
ISBN : 0849376459

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Experimental Cell Biology of Taste and Olfaction by Andrew I. Spielman,Joseph G. Brand Pdf

Experimental Cell Biology of Taste and Olfaction examines and adapts methods from a variety of established fields, such as neurophysiology, receptor biochemistry and cellular imaging to provide comprehensive coverage of current techniques and protocols in chemosensory cell biology. Written for both newcomers and established scientists, this volume offers numerous tips for problem solving and suggests ways to avoid the most common, and costly, mistakes made by researchers. This book covers general aspects such as tissue collection and preparation, as well as specific, up-to-date methods used in taste and olfactory morphology, immunology, biochemistry, biophysics, electrophysiology and molecular biology. The explosion of knowledge and the increased interest in these areas make this book an important reference work for all scientists, students, and teachers in this and related fields

Author : David W. Galbraith,Hans J. Bohnert,Don P. Bourque
Publisher : Academic Press
Page : 605 pages
File Size : 52,6 Mb
Release : 1995
Category : Medical
ISBN : 9780125641517

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Methods in Plant Cell Biology by David W. Galbraith,Hans J. Bohnert,Don P. Bourque Pdf

Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.

Author : Douglas E. Chandler,Robert W. Roberson
Publisher : Jones & Bartlett Publishers
Page : 468 pages
File Size : 45,9 Mb
Release : 2009
Category : Medical
ISBN : 9780763738747

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Bioimaging by Douglas E. Chandler,Robert W. Roberson Pdf

The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.

Author : Anonim
Publisher : Elsevier
Page : 605 pages
File Size : 55,9 Mb
Release : 1995-10-10
Category : Science
ISBN : 9780080859477

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Methods in Plant Cell Biology, Part A by Anonim Pdf

Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features* Written by experts, many of whom have developed the individual methods described* Contains most, if not all, the methods needed for modern research in plant cell biology* Up-to-date and comprehensive* Full references* Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures* Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants

Author : P. Michael Conn
Publisher : Academic Press
Page : 539 pages
File Size : 48,7 Mb
Release : 2013-10-18
Category : Science
ISBN : 9780124104709

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Receptor-Receptor Interactions by P. Michael Conn Pdf

This new volume of Methods in Cell Biology looks at receptor-receptor interactions, with sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes. With cutting-edge material, this comprehensive collection is intended to guide researchers of receptor-receptor interactions for years to come. Covers sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes Chapters are written by experts in the field Cutting-edge material

Author : Anonim
Publisher : Unknown
Page : 1844 pages
File Size : 52,6 Mb
Release : 2000
Category : Medicine
ISBN : OSU:32436010864534

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Cumulated Index Medicus by Anonim Pdf

Author : Jianghui Hou
Publisher : Academic Press
Page : 422 pages
File Size : 49,8 Mb
Release : 2020-04-24
Category : Science
ISBN : 9780128226438

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A Laboratory Guide to the Tight Junction by Jianghui Hou Pdf

A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels

Author : Rudolf A. Steinbrecht,Karl Zierold
Publisher : Springer Science & Business Media
Page : 308 pages
File Size : 53,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642728150

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Cryotechniques in Biological Electron Microscopy by Rudolf A. Steinbrecht,Karl Zierold Pdf

To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Author : John H. Moore,Nicholas D. Spencer
Publisher : Taylor & Francis
Page : 1178 pages
File Size : 47,5 Mb
Release : 2001
Category : Chemistry, Physical and theoretical
ISBN : 0750307986

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Encyclopedia of Chemical Physics and Physical Chemistry: Fundamentals by John H. Moore,Nicholas D. Spencer Pdf

Author : John H. Moore,Nicholas D. Spencer
Publisher : CRC Press
Page : 1161 pages
File Size : 50,7 Mb
Release : 2023-07-03
Category : Science
ISBN : 9781003803294

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Encyclopedia of Chemical Physics and Physical Chemistry by John H. Moore,Nicholas D. Spencer Pdf

The Encyclopedia of Physical Chemistry and Chemical Physics introduces possibly unfamiliar areas, explains important experimental and computational techniques, and describes modern endeavors. The encyclopedia quickly provides the basics, defines the scope of each subdiscipline, and indicates where to go for a more complete and detailed explanation. Particular attention has been paid to symbols and abbreviations to make this a user-friendly encyclopedia. Care has been taken to ensure that the reading level is suitable for the trained chemist or physicist. The encyclopedia is divided in three major sections: FUNDAMENTALS: the mechanics of atoms and molecules and their interactions, the macroscopic and statistical description of systems at equilibrium, and the basic ways of treating reacting systems. The contributions in this section assume a somewhat less sophisticated audience than the two subsequent sections. At least a portion of each article inevitably covers material that might also be found in a modern, undergraduate physical chemistry text. METHODS: the instrumentation and fundamental theory employed in the major spectroscopic techniques, the experimental means for characterizing materials, the instrumentation and basic theory employed in the study of chemical kinetics, and the computational techniques used to predict the static and dynamic properties of materials. APPLICATIONS: specific topics of current interest and intensive research. For the practicing physicist or chemist, this encyclopedia is the place to start when confronted with a new problem or when the techniques of an unfamiliar area might be exploited. For a graduate student in chemistry or physics, the encyclopedia gives a synopsis of the basics and an overview of the range of activities in which physical principles are applied to chemical problems. It will lead any of these groups to the salient points of a new field as rapidly as possible and gives pointers as to where to read about the topic in more detail.

Author : Andrew Harris,Darren Locke
Publisher : Springer Science & Business Media
Page : 573 pages
File Size : 50,6 Mb
Release : 2008-11-09
Category : Science
ISBN : 9781597454896

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Connexins by Andrew Harris,Darren Locke Pdf

Connexins: A Guide is a practical and valuable reference and text covering a wide scope of information about the connexin family of membrane channel proteins. The editors and contributing authors intend for this cutting-edge work to be informative to scientists wishing to learn about the field, as well as to those who are active researchers in this area. Connexins: A Guide masterfully addresses specific needs of the scientific community; it is a comprehensive and comprehensible narrative of the uncommonly diverse connexin field, making previously hard-to-find information easily accessible, while also presenting intelligible insights into the extensive experimental methods and conceptual frameworks necessary to appreciate and understand the important roles that connexin channel proteins play in health and disease.

Author : John E. Rash,C. Sue Hudson
Publisher : Unknown
Page : 226 pages
File Size : 53,9 Mb
Release : 1979
Category : Science
ISBN : UOM:39015000293806

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Freeze Fracture by John E. Rash,C. Sue Hudson Pdf

Author : John Kuo
Publisher : Springer Science & Business Media
Page : 606 pages
File Size : 47,6 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597452946

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Electron Microscopy by John Kuo Pdf

This book presents the newest technology in electron microscopy. It comprises two major areas of electron microscopy - transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. A notes section enables efficient adaptation and troubleshooting of protocols.