Recent Trends And Techniques In Plant Metabolic Engineering
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Recent Trends and Techniques in Plant Metabolic Engineering by Sudesh Kumar Yadav,Vinay Kumar,Sudhir P Singh Pdf
Remarkable research has yielded whole genome data in plants, resulting in the documentation of an ever-increasing number of genes, without establishing their functions. The huge data resources available at the genome, transcriptome, proteome and metabolome levels are of enormous value in the field of functional genomics. This book provides insights into interpreting the sea of data in order to understand basic and practical aspects of plant metabolic engineering. It discusses in detail ways to tap into this enormous pool of data to increase productivity, and offers information that is both interesting and necessary for exploring the manipulation of metabolic pathways. The interdisciplinary approaches presented here also serve as a source of ideas for practical applications.
Plant Metabolic Engineering by Vladimir Shulaev Pdf
This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant and microbial systems; and omics technologies, such as transcriptomics, proteomics, metabolomics, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Metabolic Engineering: Methods and Protocols is a valuable resource for biologists, chemists, biotechnologists, students, and broad cohorts of researchers who works in the fields of plant metabolism and metabolic engineering.
Metabolic Engineering in Plants by Tariq Aftab,Khalid Rehman Hakeem Pdf
This edited book highlights the plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Metabolic engineering is usually defined as the re-routing of one or more enzymatic reactions to generate new compounds, increase the production of existing compounds, or facilitate the degradation of compounds. Plants are the foundation of numerous compounds which are synthesized via assimilated complex biosynthetic routes. Plants have evolved an incredible arrangement of metabolic pathways leading to molecules/compounds capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which supply the ever-growing needs of humankind for natural chemicals, such as pharmaceuticals, nutraceuticals, agrochemicals, food and chemical additives, biofuels, and biomass. However, in foreseeable future we will be forced to think about the accessibility of resources for the generations to come. For these reasons, the book proposes alternative options of food/food supplement, medicines and other essential items, by using plant metabolic engineering approach. This book is of interest to teachers, researchers and academic experts. Also, the book serves as additional reading material for undergraduate and graduate students of biotechnology and molecular biology of plants.
New Frontiers in Stress Management for Durable Agriculture by Amitava Rakshit,Harikesh Bahadur Singh,Anand Kumar Singh,Uma Shankar Singh,Leonardo Fraceto Pdf
Using accessible farming practices to meet the growing demands on agriculture is likely to result in more intense competition for natural resources, increased greenhouse gas emissions, and further deforestation and land degradation, which will in turn produce additional stress in the soil-water-plant-animal continuum. Stress refers to any unfavorable force or condition that inhibits customary functioning in plants. Concurrent manifestations of different stresses (biotic and abiotic) are very frequent in the environment of plants, which consequently reduces yield. Better understanding stress not only changes our perspective on the current environment, but can also bring a wealth of benefits, like improving sustainable agriculture and human beings’ living standards. Innovative systems are called for that protect and enhance the natural resource base, while increasing productivity via ‘holistic’ approaches, such as agroecology, agro-forestry, climate-smart agriculture and conservation agriculture, which also incorporate indigenous and traditional knowledge. The book ‘New Frontiers in Stress Management for Durable Agriculture’ details the current state of knowledge and highlights scientific advances concerning novel aspects of plant biology research on stress, biotic and abiotic stress responses, as well as emergent amelioration and reclamation technologies to restore normal functioning in agroecology.
Advances in Plant Tissue Culture by Avinash Chandra Rai,Ajay Kumar,Arpan Modi,Major Singh Pdf
Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and their latest application implications. It will be beneficial for students and early-career researchers of plant sciences and plant/agricultural biotechnology. Plant tissue culture has emerged as a sustainable way to meet the requirements of fresh produces, horticultural crops, medicinal or ornamental plants. Nowadays, plant tissue culture is an emerging filed applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry. This book covers the latest technology, broadly applied for crop improvement, clonal propagation, Somatic hybridization Embryo rescue, Germplasm conservation, genetic conservation, or for the preservation of endangered species. However, these technologies also play a vital role in breaking seed dormancy over conventional methods of conservation. Focuses on plant tissue culture as an emerging field applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry Includes current studies and innovations in biotechnology Covers commercialization and current perspectives in the field of plant tissue culture techniques
Engineering of Microbial Biosynthetic Pathways by Vijai Singh,Ajay Kumar Singh,Poonam Bhargava,Madhvi Joshi,Chaitanya G. Joshi Pdf
This book provides a comprehensive overview of the basic and advanced metabolic engineering technologies used to generate natural metabolites and industrially important biomolecules. Metabolic engineering has the potential to produce large quantities of valuable biomolecules in a renewable and sustainable manner by extending or modifying biosynthetic pathways in a wide range of organisms. It has been successfully used to produce chemicals, drugs, enzymes, amino acids, antibiotics, biofuels, and industrially important pharmaceuticals. The book comprehensively reviews the various metabolites detection, extraction and biosensors and the metabolic engineering of microbial strains for the production of industrially useful enzymes, proteins, organic acids, vitamins and antibiotics, therapeutics, chemicals, and biofuels. It also discusses various genetic engineering and synthetic biology tools for metabolic engineering. In closing, the book discusses ethical, patenting and regulatory issues in the metabolic engineering of microbes. This book is a valuable source not only for beginners in metabolic engineering, but also students, researchers, biotechnology and metabolic engineering based company.
In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.
Metabolic Engineering by Sang Yup Lee,Jens Nielsen,Gregory Stephanopoulos Pdf
Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.
Plant Secondary Metabolism Engineering by Arthur Germano Fett-Neto Pdf
This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols. Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field.
Synthetic Biology and Metabolic Engineering in Plants and Microbes Part B: Metabolism in Plants by Anonim Pdf
Synthetic Biology and Metabolic Engineering in Plants and Microbes, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the usage of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways. Continues the legacy of this premier serial with quality chapters authored by leaders in the field of enzymology Contains two volumes covering research methods in synthetic biology and metabolic engineering in plants and microbes Includes sections on such topics as the uses of integrases in microbial engineering, biosynthesis and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways
Yeast Metabolic Engineering by Valeria Mapelli,Maurizio Bettiga Pdf
This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
Nutraceuticals Production from Plant Cell Factory by Tarun Belwal,Milen I. Georgiev,Jameel M Al-Khayri Pdf
This book focuses on in vitro techniques and challenges of producing nutraceutical compounds from plant cells. In addition, it provides an overview of different biosynthesis pathways and their modulation through cell culture techniques for the production of nutraceutical compounds in high quantity and quality. It also includes the assessment of the factors influencing production and advances in cell culture techniques, including the scale-up approach using bioreactors. Lastly it provides valuable suggestion for future research.
This book highlights the advances in essential oil research, from the plant physiology perspective to large-scale production, including bioanalytical methods and industrial applications. The book is divided into 4 sections. The first one is focused on essential oil composition and why plants produce these compounds that have been used by humans since ancient times. Part 2 presents an update on the use of essential oils in various areas, including food and pharma industries as well as agriculture. In part 3 readers will find new trends in bioanalytical methods. Lastly, part 4 presents a number of approaches to increase essential oil production, such as in vitro and hairy root culture, metabolic engineering and biotechnology. Altogether, this volume offers a comprehensive look at what researchers have been doing over the last years to better understand these compounds and how to explore them for the benefit of the society.
Plant-Microbial Interactions and Smart Agricultural Biotechnology by Swati Tyagi,Robin Kumar,Baljeet Saharan,Ashok Kumar Nadda Pdf
Considering the ever-increasing global population and finite arable land, technology and sustainable agricultural practices are required to improve crop yield. This book examines the interaction between plants and microbes and considers the use of advanced techniques such as genetic engineering, revolutionary gene editing technologies, and their applications to understand how plants and microbes help or harm each other at the molecular level. Understanding plant-microbe interactions and related gene editing technologies will provide new possibilities for sustainable agriculture. The book will be extremely useful for researchers working in the fields of plant science, molecular plant biology, plant-microbe interactions, plant engineering technology, agricultural microbiology, and related fields. It will be useful for upper-level students and instructors specifically in the field of biotechnology, microbiology, biochemistry, and agricultural science. Features: Examines the most advanced approaches for genetic engineering of agriculture (CRISPR, TALAN, ZFN, etc.). Discusses the microbiological control of various plant diseases. Explores future perspectives for research in microbiological plant science. Plant-Microbial Interactions and Smart Agricultural Biotechnology will serve as a useful source of cutting-edge information for researchers and innovative professionals, as well as upper-level undergraduate and graduate students taking related agriculture and environmental science courses.
The Future of Metabolic Engineering by Abhishek Sharma Pdf
The last two decades of scientific research have progressed to the point where metabolic engineering, which involves the modifying of metabolic pathways of animals, plants, and bacterial cells at biochemical and molecular levels, is feasible. Conceptually, metabolic engineering takes into account the identification of major blocks or control points in a metabolic pathway at the molecular level followed by removal of these limitations with the help of various cellular engineering interventions. Understanding the metabolic pathways requires appropriate experiment setup, molecular biology and biochemistry methods, computational modelling, data analysis, and interpretation to allow the researchers to manipulate them as per their needs. This book comprises a total of 12 chapters from multiple contributors of different countries around the world, including Brazil, Egypt, India, Saudi Arabia, and Turkey. This book provides deep insight into the past, present, and future of metabolic engineering in the animal, microbial, and plant system, communicating interdisciplinary research and relevant results in biochemistry, molecular biology, applied microbiology, cellular physiology, cellular nutrition in health and disease, and biochemical engineering.
