Sedimentation Equilibrium Analytical Ultracentrifugation

Author : Peter Schuck,Rodolfo Ghirlando
Publisher : CRC Press
Page : 200 pages
File Size : 49,9 Mb
Release : 2019-08-26
Category : Electronic
ISBN : 1498768865

Get Book

Sedimentation Equilibrium Analytical Ultracentrifugation by Peter Schuck,Rodolfo Ghirlando Pdf

A follow-up to the experimental and instrumental aspects described in Basic Principles of Analytical Ultracentrifugation, the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles describes the theory and practice of data analysis. Mathematical models for the sedimentation process and the evolution of detected signals are developed in a comprehensive framework, jointly with the description of current and historical strategies for how to extract from noisy experimental data the physical parameters of interest, such as size, mass, and shape, composition, and polydispersity of sedimenting particles. The methods are extensively illustrated, and supported with practical applications, as well as cross-references where to find the methods in the public domain software SEDFIT and SEDPHAT. The systems covered are discrete or polydisperse mixtures of sedimenting molecules or particles in dilute solution, such as proteins and other biomolecules and their stable complexes, man-made polymers, and nanoparticles, observed in different optical systems. These methods form the essential foundation for the analysis of dynamic interacting systems, which are covered in a separate volume.

Author : Stephen E Harding,David Scott,Arther Rowe
Publisher : Royal Society of Chemistry
Page : 612 pages
File Size : 51,8 Mb
Release : 2007-12-16
Category : Science
ISBN : 9781847552617

Get Book

Analytical Ultracentrifugation by Stephen E Harding,David Scott,Arther Rowe Pdf

Analytical ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.

Author : Susumu Uchiyama,Fumio Arisaka,Walter F. Stafford,Tom Laue
Publisher : Springer
Page : 532 pages
File Size : 47,5 Mb
Release : 2016-05-13
Category : Science
ISBN : 9784431559856

Get Book

Analytical Ultracentrifugation by Susumu Uchiyama,Fumio Arisaka,Walter F. Stafford,Tom Laue Pdf

This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC. This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included. The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis.

Author : W. Borchard,A. Straatmann
Publisher : Springer Science & Business Media
Page : 168 pages
File Size : 40,7 Mb
Release : 2002-02-11
Category : Science
ISBN : 9783540424895

Get Book

Analytical Ultracentrifugation VI by W. Borchard,A. Straatmann Pdf

This volume includes 20 contributions of the 12th meeting on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. Various fields of ultracentrifugation are covered concerning research problems in biochemistry, biophysical chemistry and macromolecular chemistry as well as interacting systems. New investigations concerning the sedimentation theory are presented. The phase transition of gels is dealt with, as is the sedimentation-diffusion equilibrium of gels. One section contains the hydrodynamics of biopolymers.

Author : Todd M. Schuster,Thomas M. Laue
Publisher : Springer Science & Business Media
Page : 359 pages
File Size : 44,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468468281

Get Book

Modern Analytical Ultracentrifugation by Todd M. Schuster,Thomas M. Laue Pdf

There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.

Author : Peter Schuck,Huaying Zhao,Chad A. Brautigam,Rodolfo Ghirlando
Publisher : CRC Press
Page : 302 pages
File Size : 53,7 Mb
Release : 2016-01-05
Category : Medical
ISBN : 9781498751162

Get Book

Basic Principles of Analytical Ultracentrifugation by Peter Schuck,Huaying Zhao,Chad A. Brautigam,Rodolfo Ghirlando Pdf

Analytical ultracentrifugation (AUC) can supply rich information on the mass, shape, size distribution, solvation, and composition of macromolecules and nanoscopic particles. It also provides a detailed view of their reversible single- or multi-component interactions over a wide range of affinities. Yet this powerful technique has been hard to master in mainstream molecular sciences due to a lack of comprehensive books on the subject. Filling this gap in the literature of biophysical methodology, Basic Principles of Analytical Ultracentrifugation explains the fundamentals in the theory and practice of AUC. The book provides you with up-to-date experimental information to confidently practice AUC. You will understand the basic concepts, full potential, and possible pitfalls of AUC as well as appreciate the current relevance of past work in the field. The book first introduces the basic principles and technical setup of an AUC experiment and briefly describes the optical systems used for detection. It then explores the ultracentrifugation experiment from a macromolecular standpoint, offering a detailed physical picture of the sedimentation process and relevant macromolecular parameters. The authors present important practical aspects for conducting an experiment, including sample preparation, data acquisition and data structure, and the execution of the centrifugal experiment. They also cover instrument calibration and quality control experiments.

Author : Peter Schuck,Huaying Zhao
Publisher : CRC Press
Page : 230 pages
File Size : 47,5 Mb
Release : 2017-09-22
Category : Science
ISBN : 9781351976831

Get Book

Sedimentation Velocity Analytical Ultracentrifugation by Peter Schuck,Huaying Zhao Pdf

Analytical ultracentrifugation is one of the most powerful solution techniques for the study of macromolecular interactions, to define the number and stoichiometry of complexes formed, and to measure affinities ranging from very strong to very weak and repulsive. Building on the data analysis tools described in the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles, and the experimental and instrumental aspects in the first volume Basic Principles of Analytical Ultracentrifugation, the present volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems is devoted to the theory and practical data analysis of dynamically coupled sedimentation processes. This volume is designed to fill a gap in biophysical methodology to provide a framework that builds on the fundamentals of the highly developed traditional methods of analytical ultracentrifugation, updated with current methodology and from a viewpoint of modern applications. It will be an invaluable resource for researchers and graduate students interested in the application of analytical ultracentrifugation in the study of interacting systems, such as biological macromolecules, multi-protein complexes, polymers, or nanoparticles.

Author : M. D. Lechner,Lars Börger
Publisher : Springer Science & Business Media
Page : 180 pages
File Size : 48,8 Mb
Release : 2004-11-24
Category : Science
ISBN : 354020203X

Get Book

Analytical Ultracentrifugation VII by M. D. Lechner,Lars Börger Pdf

This volume includes 19 contributions to the 13th International Symposium on Analytical Ultracentrifugation which took place at the university of Osnabrück on March 6th and 7th, 2003. The contributions from leading scientists cover a broad spectrum of topics concerning: Technical Methods, Data Analysis, Innovations; Polymers, Colloids, Supramolecular Systems; Biological and Interaction Systems; Hydrodynamics and Modelling. Due to the increasing significance of Analytical Ultracentrifugation for both scientific and technical applications, this book will be an essential source of information with respect to recent developments and results related to this important analytical method.

Author : W. Borchard
Publisher : Unknown
Page : 136 pages
File Size : 49,8 Mb
Release : 1991
Category : Technology & Engineering
ISBN : UCSD:31822015323322

Get Book

Progress in Analytical Ultracentrifugation by W. Borchard Pdf

Author : Peter Schuck
Publisher : Springer Science & Business Media
Page : 537 pages
File Size : 49,8 Mb
Release : 2007-03-20
Category : Science
ISBN : 9780387359663

Get Book

Protein Interactions by Peter Schuck Pdf

This volume successfully and clearly examines how biophysical approaches can be used to study complex systems of reversibly interacting proteins. It deals with the methodology behind the research and shows how to synergistically incorporate several methodologies for use. Each chapter treats and introduces the reader to different biological systems, includes a brief summary of the physical principles, and mentions practical requirements.

Author : H. Cölfen
Publisher : Springer
Page : 223 pages
File Size : 41,5 Mb
Release : 2003-07-01
Category : Technology & Engineering
ISBN : 9783540487036

Get Book

Analytical Ultracentrifugation V by H. Cölfen Pdf

The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including: Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies. In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare. Therefore, this volume will be a helpful source of information to anyone who wants to catch up with the most recent developments and results related to this important analytical method.

Author : Stephen E. Harding,Arthur J. Rowe,John C. Horton
Publisher : Unknown
Page : 656 pages
File Size : 55,5 Mb
Release : 1992
Category : Science
ISBN : STANFORD:36105002260789

Get Book

Analytical Ultracentrifugation in Biochemistry and Polymer Science by Stephen E. Harding,Arthur J. Rowe,John C. Horton Pdf

This is the first book of its kind to appear for nearly two decades and gives as comprehensive a coverage as is possible of the present state-of-the-art.

Author : Theodor Svedberg,Kai Oluf Pedersen,Johannes Henrik Bauer
Publisher : Unknown
Page : 566 pages
File Size : 55,6 Mb
Release : 1959
Category : Centrifugation
ISBN : UOM:39015004620756

Get Book

The Ultracentrifuge by Theodor Svedberg,Kai Oluf Pedersen,Johannes Henrik Bauer Pdf

Author : J. Behlke
Publisher : Springer
Page : 228 pages
File Size : 51,6 Mb
Release : 1995-11-20
Category : Science
ISBN : UCSD:31822009014655

Get Book

Analytical Ultracentrifugation by J. Behlke Pdf

The 9th Symposium on Analytical Ultracentrifugation (AUC) which was held at the Max DelbrA1/4ck Center for Molecular Medicine in Berlin-Buch, March, 1995, is the basis of this volume. It presents an actual survey of recent developments and results of ultracentrifugation methods in the frame of the following topics: Reversible Association Reactions.- Analysis of Supramolecular Structures Including Modeling.- Size Distribution Analysis and Gel Formation.- Hardware and Software Developments.

Author : Rainer Jaenicke,Helmut Durchschlag
Publisher : Springer
Page : 212 pages
File Size : 40,7 Mb
Release : 1997-11
Category : Science
ISBN : UCSD:31822025565326

Get Book

Analytical Ultracentrifugation IV by Rainer Jaenicke,Helmut Durchschlag Pdf

This volume contains the contributions of the 10th Symposium on Analytical Ultracentrifugation, held in Regensburg, Germany, on March 13th to 14th, 1997. Topics in the following fields were presented: Technical innovations; innovations in data analysis; modeling; biological systems; ions and polyelectrolytes; polymers, colloids, supramolecular systems; emulsions, gels, and dispersions; interacting systems and assemblies.