High Dimensional Single Cell Analysis

Author : Harris G. Fienberg,Garry P. Nolan
Publisher : Springer
Page : 224 pages
File Size : 48,9 Mb
Release : 2014-04-22
Category : Medical
ISBN : 9783642548277

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High-Dimensional Single Cell Analysis by Harris G. Fienberg,Garry P. Nolan Pdf

This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is an invaluable reference book for both basic and clinical researchers.

Author : Karsten Kuritz
Publisher : Logos Verlag Berlin GmbH
Page : 150 pages
File Size : 43,9 Mb
Release : 2020-11-20
Category : Language Arts & Disciplines
ISBN : 9783832552091

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Analysis and Control of Cellular Ensembles. Exploiting dimensionality reduction in single-cell data and models by Karsten Kuritz Pdf

An ensemble system is a collection of nearly identical dynamical systems which admit a certain degree of heterogeneity, and which are subject to the restriction that they may only be manipulated or observed as a whole. This thesis presents analysis and control methods for cellular ensembles by considering reduced 1-dimensional dynamics of biological processes in high-dimensional single-cell data and models. To be more specific, we address the quest for real-time analysis of biological processes within single-cell data by introducing the measure-preserving map of pseudotime into real-time, in short MAPiT. MAPiT enables the reconstruction of temporal and spatial dynamics from single-cell snapshot experiments. In addition, we propose a PDE-constrained learning algorithm which allows for efficient inference of changes in cell cycle progression from time series single-cell snapshot data. The second part of this thesis, is devoted to controlling a heterogeneous cell population, in the sense, that we aim at achieving a desired distribution of cellular oscillators on their periodic orbit. A systems theoretic approach to the ensemble control problem provides novel necessary and sufficient conditions for the control of phase distributions in terms of the Fourier coefficients of the phase response curve. This thesis establishes a connection between the previously separate areas of single cell analysis and ensemble control. Our holistic view opens new perspectives for theoretic concepts in basic research and therapeutic strategies in precision medicine.

Author : Xinkun Wang
Publisher : CRC Press
Page : 435 pages
File Size : 47,6 Mb
Release : 2023-07-06
Category : Mathematics
ISBN : 9781000897197

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Next-Generation Sequencing Data Analysis by Xinkun Wang Pdf

RNA-seq: both bulk and single-cell (separate chapters) Genotyping and variant discovery through whole genome/exome sequencing Clinical sequencing and detection of actionable variants De novo genome assembly ChIP-seq to map protein-DNA interactions Epigenomics through DNA methylation sequencing Metagenome sequencing for microbiome analysis

Author : Fan-Gang Tseng,Tuhin Subhra Santra
Publisher : Springer
Page : 414 pages
File Size : 51,7 Mb
Release : 2016-01-21
Category : Technology & Engineering
ISBN : 9783662491188

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Essentials of Single-Cell Analysis by Fan-Gang Tseng,Tuhin Subhra Santra Pdf

This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.

Author : Masahiro Ono
Publisher : Springer Nature
Page : 276 pages
File Size : 42,5 Mb
Release : 2022-09-30
Category : Medical
ISBN : 9781071626474

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Regulatory T-Cells by Masahiro Ono Pdf

This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Regulatory T-Cells: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Author : Carlo Pellicciari,Marco Biggiogera,Manuela Malatesta
Publisher : Springer Nature
Page : 351 pages
File Size : 55,8 Mb
Release : 2022-09-24
Category : Science
ISBN : 9781071626757

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Histochemistry of Single Molecules by Carlo Pellicciari,Marco Biggiogera,Manuela Malatesta Pdf

This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Author : Tuhin Subhra Santra,Fan-Gang Tseng
Publisher : MDPI
Page : 254 pages
File Size : 54,8 Mb
Release : 2021-06-02
Category : Science
ISBN : 9783036506289

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Single Cell Analysis by Tuhin Subhra Santra,Fan-Gang Tseng Pdf

Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.

Author : Jianqin Gu,Xiangdong Wang
Publisher : Springer
Page : 195 pages
File Size : 50,7 Mb
Release : 2018-06-25
Category : Science
ISBN : 9789811305023

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Single Cell Biomedicine by Jianqin Gu,Xiangdong Wang Pdf

The book focuses on various detection targets applied in single cell studies, including tumor tissue cells, circulating tumor cells (CTCs), disseminated tumor cells (DTCs), circulating tumor DNA (ctDNA), cell-free DNA (cfDNA) and cancer stem cells (CSCs). It also discusses and compares detection methods using these detection targets in different fields to reveal single cell biomedical functions. The volume focuses not only on the methods already been established and validated, and also the methods newly developed. The book also highlights the importance and potential of single cell biomedicine in the development and validation of precision medicine strategies. It is useful for researchers and students in the field of cell biology, molecular medicine and precision medicine etc.

Author : Said Dermime,Maysaloun Merhi,Taha Merghoub
Publisher : Frontiers Media SA
Page : 284 pages
File Size : 46,7 Mb
Release : 2021-12-28
Category : Medical
ISBN : 9782889718634

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Dynamic Biomarkers of Response to Anti-Immune Checkpoint Inhibitors in Cancer by Said Dermime,Maysaloun Merhi,Taha Merghoub Pdf

Author : Chiara Romualdi,Enrica Calura,Davide Risso,Sampsa Hautaniemi,Francesca Finotello
Publisher : Frontiers Media SA
Page : 187 pages
File Size : 43,8 Mb
Release : 2020-12-03
Category : Medical
ISBN : 9782889661510

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Multi-omic Data Integration in Oncology by Chiara Romualdi,Enrica Calura,Davide Risso,Sampsa Hautaniemi,Francesca Finotello Pdf

This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Author : Andreas Pircher,Dominik Wolf
Publisher : Frontiers Media SA
Page : 254 pages
File Size : 42,6 Mb
Release : 2022-08-19
Category : Medical
ISBN : 9782889768295

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New Horizons in Tumor Microenvironment Biology and Therapy: Implications for New Therapies Vol. II by Andreas Pircher,Dominik Wolf Pdf

Author : Daniel R. Catchpoole,Simeon J. Simoff,Paul J Kennedy,Quang Vinh Nguyen
Publisher : CRC Press
Page : 221 pages
File Size : 46,7 Mb
Release : 2023-12-06
Category : Medical
ISBN : 9781003801689

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Data Driven Science for Clinically Actionable Knowledge in Diseases by Daniel R. Catchpoole,Simeon J. Simoff,Paul J Kennedy,Quang Vinh Nguyen Pdf

Data-driven science has become a major decision-making aid for the diagnosis and treatment of disease. Computational and visual analytics enables effective exploration and sense making of large and complex data through the deployment of appropriate data science methods, meaningful visualisation and human-information interaction. This edited volume covers state-of-the-art theory, method, models, design, evaluation and applications in computational and visual analytics in desktop, mobile and immersive environments for analysing biomedical and health data. The book is focused on data-driven integral analysis, including computational methods and visual analytics practices and solutions for discovering actionable knowledge in support of clinical actions in real environments. By studying how data and visual analytics have been implemented into the healthcare domain, the book demonstrates how analytics influences the domain through improving decision making, specifying diagnostics, selecting the best treatments and generating clinical certainty.

Author : Dongya Jia,Yapeng Su,Mingyang Lu,Xuefei Li
Publisher : Frontiers Media SA
Page : 153 pages
File Size : 52,6 Mb
Release : 2023-06-21
Category : Medical
ISBN : 9782832526965

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Systems Biology and Single-cell Analysis of Cancer Metabolism and its Role in Cancer Emergent Properties by Dongya Jia,Yapeng Su,Mingyang Lu,Xuefei Li Pdf

Author : Anonim
Publisher : Academic Press
Page : 218 pages
File Size : 48,9 Mb
Release : 2022-04-20
Category : Science
ISBN : 9780323907422

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One, No one, One Hundred Thousand - The Multifaceted Role of Macrophages in Health and Disease - Part A by Anonim Pdf

One, No one, One Hundred Thousand - The Multifaceted Role of Macrophages in Health and Disease - Part A, provides in-depth reviews on the last progresses on the role of macrophages in health and diseases with a special focus on the role of macrophages in SARS-COV2, breast cancer, liver and salivary glands. Provides accurate reviews from selected experts on the topic of macrophages in different tissues such as lung, uterus, breast and liver Each chapter of the volume provides useful graphic material for ease of reading of the audience Provides the latest insights and future perspectives on macrophage heterogeneity in different diseases

Author : Lars A. Akslen,Randolph S. Watnick
Publisher : Springer
Page : 534 pages
File Size : 48,5 Mb
Release : 2017-08-02
Category : Medical
ISBN : 9783319391472

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Biomarkers of the Tumor Microenvironment by Lars A. Akslen,Randolph S. Watnick Pdf

This book reviews different aspects of the cancer microenvironment, and its regulation and importance for tumor progression. Practical applications, in terms of how biomarkers are increasingly included in therapy protocols, will also be discussed. Biomarkers of the Tumor Microenvironment: Basic Studies and Practical Applications is aimed at research pathologists in the cancer field, and also cancer researchers from other backgrounds, especially those using morphology techniques and models focusing on cross-talk between different cell types in tumors.