Single Molecule Enzymology

Author : Gregory I. Mashanov,Christopher Batters
Publisher : Humana Press
Page : 294 pages
File Size : 47,5 Mb
Release : 2016-08-23
Category : Science
ISBN : 149396190X

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Single Molecule Enzymology by Gregory I. Mashanov,Christopher Batters Pdf

The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.

Author : Anonim
Publisher : Academic Press
Page : 616 pages
File Size : 47,6 Mb
Release : 2016-10-28
Category : Medical
ISBN : 9780128095478

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Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods by Anonim Pdf

Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion

Author : Anonim
Publisher : Academic Press
Page : 484 pages
File Size : 45,8 Mb
Release : 2017-01-03
Category : Science
ISBN : 9780128095034

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Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods by Anonim Pdf

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion

Author : Anonim
Publisher : Academic Press
Page : 528 pages
File Size : 51,8 Mb
Release : 2010-08-17
Category : Science
ISBN : 008095927X

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Single Molecule Tools, Part A: Fluorescence Based Approaches by Anonim Pdf

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Author : David Bensimon,Vincent Croquette,Jean-François Allemand,Xavier Michalet,Terence Strick
Publisher : Oxford University Press
Page : 224 pages
File Size : 46,6 Mb
Release : 2018-11-30
Category : Science
ISBN : 9780192559968

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Single-Molecule Studies of Nucleic Acids and Their Proteins by David Bensimon,Vincent Croquette,Jean-François Allemand,Xavier Michalet,Terence Strick Pdf

This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.

Author : Toshio Yanagida,Yoshiharu Ishii
Publisher : John Wiley & Sons
Page : 347 pages
File Size : 45,6 Mb
Release : 2008-11-24
Category : Science
ISBN : 9783527312887

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Single Molecule Dynamics in Life Science by Toshio Yanagida,Yoshiharu Ishii Pdf

In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.

Author : Peter Hinterdorfer,Antoine van Oijen
Publisher : Springer Science & Business Media
Page : 634 pages
File Size : 50,8 Mb
Release : 2009-12-24
Category : Science
ISBN : 9780387764979

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Handbook of Single-Molecule Biophysics by Peter Hinterdorfer,Antoine van Oijen Pdf

This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Author : Alexander E. Knight
Publisher : Academic Press
Page : 369 pages
File Size : 45,9 Mb
Release : 2009-02-26
Category : Science
ISBN : 9780080921235

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Single Molecule Biology by Alexander E. Knight Pdf

Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. Organized by biological system or molecule Each chapter discusses insights into mechanism of action, structure, and function Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc Includes an introduction to key principles and an historical overview Discusses applications in molecular diagnostics and drug discovery Provides an expert's perspective on future development

Author : Hong Qian,Hao Ge
Publisher : Springer Nature
Page : 364 pages
File Size : 41,8 Mb
Release : 2021-10-18
Category : Mathematics
ISBN : 9783030862527

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Stochastic Chemical Reaction Systems in Biology by Hong Qian,Hao Ge Pdf

This book provides an introduction to the analysis of stochastic dynamic models in biology and medicine. The main aim is to offer a coherent set of probabilistic techniques and mathematical tools which can be used for the simulation and analysis of various biological phenomena. These tools are illustrated on a number of examples. For each example, the biological background is described, and mathematical models are developed following a unified set of principles. These models are then analyzed and, finally, the biological implications of the mathematical results are interpreted. The biological topics covered include gene expression, biochemistry, cellular regulation, and cancer biology. The book will be accessible to graduate students who have a strong background in differential equations, the theory of nonlinear dynamical systems, Markovian stochastic processes, and both discrete and continuous state spaces, and who are familiar with the basic concepts of probability theory.

Author : Anonim
Publisher : Academic Press
Page : 720 pages
File Size : 48,9 Mb
Release : 2010-07-09
Category : Science
ISBN : 0123814839

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Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods by Anonim Pdf

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Author : R. Rigler,M. Orrit,T. Basche
Publisher : Springer Science & Business Media
Page : 375 pages
File Size : 52,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642565441

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Single Molecule Spectroscopy by R. Rigler,M. Orrit,T. Basche Pdf

The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.

Author : Astrid Graslund
Publisher : Springer Science & Business Media
Page : 570 pages
File Size : 43,7 Mb
Release : 2010-04-17
Category : Molecular spectroscopy
ISBN : 9783642025983

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Single Molecule Spectroscopy in Chemistry, Physics and Biology by Astrid Graslund Pdf

Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.

Author : Susanta K Sarkar
Publisher : Morgan & Claypool Publishers
Page : 74 pages
File Size : 44,5 Mb
Release : 2016-04-01
Category : Science
ISBN : 9781681741161

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Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics by Susanta K Sarkar Pdf

This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.

Author : Christoph Bräuchle,Don Carroll Lamb,Jens Michaelis
Publisher : John Wiley & Sons
Page : 359 pages
File Size : 44,5 Mb
Release : 2009-10-30
Category : Science
ISBN : 3527628371

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Single Particle Tracking and Single Molecule Energy Transfer by Christoph Bräuchle,Don Carroll Lamb,Jens Michaelis Pdf

Closing a gap in the literature, this handbook gathers all the information on single particle tracking and single molecule energy transfer. It covers all aspects of this hot and modern topic, from detecting virus entry to membrane diffusion, and from protein folding using spFRET to coupled dye systems, as well recent achievements in the field. Throughout, the first-class editors and top international authors present content of the highest quality, making this a must-have for physical chemists, spectroscopists, molecular physicists and biochemists.

Author : Anonim
Publisher : Unknown
Page : 663 pages
File Size : 44,7 Mb
Release : 2010
Category : Electronic
ISBN : OCLC:672240143

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Single Molecule Tools by Anonim Pdf