Single Molecule Tools Part A Fluorescence Based Approaches

Author : Anonim
Publisher : Academic Press
Page : 528 pages
File Size : 47,8 Mb
Release : 2010-08-17
Category : Science
ISBN : 008095927X

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Single Molecule Tools, Part A: Fluorescence Based Approaches by Anonim Pdf

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Author : Anonim
Publisher : Academic Press
Page : 720 pages
File Size : 41,5 Mb
Release : 2010-07-09
Category : Science
ISBN : 0123814839

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Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods by Anonim Pdf

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Author : Nils G. Walter
Publisher : Unknown
Page : 476 pages
File Size : 41,9 Mb
Release : 2010
Category : Biosensors
ISBN : OCLC:781295982

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Single Molecule Tools by Nils G. Walter Pdf

Author : Anonim
Publisher : Academic Press
Page : 616 pages
File Size : 43,9 Mb
Release : 2016-10-28
Category : Medical
ISBN : 9780128095478

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Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods by Anonim Pdf

Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion

Author : Iddo Heller,David Dulin,Erwin J.G. Peterman
Publisher : Humana
Page : 0 pages
File Size : 48,5 Mb
Release : 2023-11-09
Category : Science
ISBN : 1071633767

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Single Molecule Analysis by Iddo Heller,David Dulin,Erwin J.G. Peterman Pdf

This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Nils G. Walter
Publisher : Unknown
Page : 663 pages
File Size : 46,9 Mb
Release : 2010
Category : Enzymology
ISBN : 0123814820

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Methods in Enzymology by Nils G. Walter Pdf

Author : Anonim
Publisher : Unknown
Page : 663 pages
File Size : 43,9 Mb
Release : 2010
Category : Electronic
ISBN : OCLC:672240143

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Single Molecule Tools by Anonim Pdf

Author : Anonim
Publisher : Academic Press
Page : 645 pages
File Size : 44,8 Mb
Release : 2011-02-28
Category : Medical
ISBN : 9780123812759

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Fragment Based Drug Design by Anonim Pdf

There are numerous excellent reviews on fragment-based drug discovery (FBDD), but there are to date no hand-holding guides or protocols with which one can embark on this orthogonal approach to complement traditional high throughput screening methodologies. This Methods in Enzymology volume offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens. The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD. Also elaborated by experienced researchers in FBDD are sample preparations of fragments, proteins, and GPCR as well as examples of how to generate leads from hits. Offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD

Author : Roland K. Hartmann,Albrecht Bindereif,Astrid Schön,Eric Westhof
Publisher : John Wiley & Sons
Page : 1368 pages
File Size : 42,5 Mb
Release : 2015-10-06
Category : Science
ISBN : 9783527650545

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Handbook of RNA Biochemistry by Roland K. Hartmann,Albrecht Bindereif,Astrid Schön,Eric Westhof Pdf

The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.

Author : Anonim
Publisher : Academic Press
Page : 668 pages
File Size : 45,6 Mb
Release : 2011-09-19
Category : Science
ISBN : 9780123851192

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Methods in Systems Biology by Anonim Pdf

Systems biology is a term used to describe a number of trends in bioscience research and a movement that draws on those trends. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology. With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research

Author : Chris Voigt
Publisher : Academic Press
Page : 714 pages
File Size : 40,5 Mb
Release : 2011-07-08
Category : Science
ISBN : 9780123850768

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Synthetic Biology, Part A by Chris Voigt Pdf

Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. Encompasses a variety of different approaches, methodologies and disciplines Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation

Author : Anonim
Publisher : Academic Press
Page : 380 pages
File Size : 53,8 Mb
Release : 2010-09-30
Category : Science
ISBN : 0123849942

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Cryo-EM, Part C by Anonim Pdf

This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. * Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

Author : Anonim
Publisher : Academic Press
Page : 608 pages
File Size : 41,8 Mb
Release : 2011-02-17
Category : Science
ISBN : 9780123812957

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Research on Nitrification and Related Processes by Anonim Pdf

State-of-the-art update on methods and protocols dealing with the detection, isolation and characterization of macromolecules and their hosting organisms that facilitate nitrification and related processes in the nitrogen cycle as well as the challenges of doing so in very diverse environments. Provides state-of-the-art update on methods and protocols Deals with the detection, isolation and characterization of macromolecules and their hosting organisms deals with the challenges of very diverse environments.

Author : Anonim
Publisher : Academic Press
Page : 708 pages
File Size : 46,6 Mb
Release : 2010-11-24
Category : Science
ISBN : 0123812976

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Constitutive Activity in Receptors and Other Proteins by Anonim Pdf

This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods

Author : Anonim
Publisher : Academic Press
Page : 464 pages
File Size : 50,8 Mb
Release : 2011-02-14
Category : Science
ISBN : 9780123859297

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The Unfolded Protein Response and Cellular Stress by Anonim Pdf

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.