Single Molecule Enzymology Fluorescence Based And High Throughput Methods

Author : Anonim
Publisher : Academic Press
Page : 616 pages
File Size : 55,9 Mb
Release : 2016-10-28
Category : Medical
ISBN : 9780128095478

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Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods by Anonim Pdf

Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion

Author : Anonim
Publisher : Academic Press
Page : 484 pages
File Size : 54,9 Mb
Release : 2017-01-03
Category : Science
ISBN : 9780128095034

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Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods by Anonim Pdf

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion

Author : Anonim
Publisher : Academic Press
Page : 528 pages
File Size : 43,9 Mb
Release : 2010-08-17
Category : Science
ISBN : 008095927X

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Single Molecule Tools, Part A: Fluorescence Based Approaches by Anonim Pdf

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Author : Gregory I. Mashanov,Christopher Batters
Publisher : Humana Press
Page : 294 pages
File Size : 40,7 Mb
Release : 2011-08-04
Category : Science
ISBN : 1617792608

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Single Molecule Enzymology by Gregory I. Mashanov,Christopher Batters Pdf

The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.

Author : Anonim
Publisher : Academic Press
Page : 451 pages
File Size : 54,6 Mb
Release : 2019-01-25
Category : Science
ISBN : 9780128167618

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CRISPR-Cas Enzymes by Anonim Pdf

CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Updated release includes the latest information on the CRISPR-Cas Enzymes

Author : Nils G. Walter
Publisher : Unknown
Page : 476 pages
File Size : 54,9 Mb
Release : 2010
Category : Biosensors
ISBN : OCLC:781295982

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Single Molecule Tools by Nils G. Walter Pdf

Author : Iddo Heller,David Dulin,Erwin J.G. Peterman
Publisher : Springer Nature
Page : 511 pages
File Size : 52,6 Mb
Release : 2023-11-13
Category : Science
ISBN : 9781071633779

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Single Molecule Analysis by Iddo Heller,David Dulin,Erwin J.G. Peterman Pdf

This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Sebastian Hiller,Maili Liu,Lichun He
Publisher : Royal Society of Chemistry
Page : 413 pages
File Size : 42,8 Mb
Release : 2023-11-01
Category : Science
ISBN : 9781839162824

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Biophysics of Molecular Chaperones by Sebastian Hiller,Maili Liu,Lichun He Pdf

Author : Jean-Louis Reymond
Publisher : John Wiley & Sons
Page : 386 pages
File Size : 53,5 Mb
Release : 2006-05-12
Category : Science
ISBN : 9783527607211

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Enzyme Assays by Jean-Louis Reymond Pdf

Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years. For biochemists, analytical, organic and catalytic chemists, and biotechnologists.

Author : Anonim
Publisher : Elsevier
Page : 7944 pages
File Size : 40,8 Mb
Release : 2010-03-05
Category : Science
ISBN : 9780080453828

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Comprehensive Natural Products II by Anonim Pdf

This work presents a definitive interpretation of the current status of and future trends in natural products—a dynamic field at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids, and enzymes. With more than 1,800 color figures, Comprehensive Natural Products II features 100% new material and complements rather than replaces the original work (©1999). Reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine Stimulates new ideas among the established natural products research community—which includes chemists, biochemists, biologists, botanists, and pharmacologists Informs and inspires students and newcomers to the field with accessible content in a range of delivery formats Includes 100% new content, with more than 6,000 figures (1/3 of these in color) and 40,000 references to the primary literature, for a thorough examination of the field Highlights new research and innovations concerning living organisms and their distinctive role in our understanding and improvement of human health, genomics, ecology/environment, and more Adds to the rich body of work that is the first edition, which will be available for the first time in a convenient online format giving researchers complete access to authoritative Natural Products content

Author : Huan-Xiang Zhou,Jan-Hendrik Spille,Priya R. Banerjee
Publisher : Springer Nature
Page : 454 pages
File Size : 54,6 Mb
Release : 2022-10-13
Category : Science
ISBN : 9781071626634

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Phase-Separated Biomolecular Condensates by Huan-Xiang Zhou,Jan-Hendrik Spille,Priya R. Banerjee Pdf

This volume provides readers with a broad collection of theoretical, computational, and experimental methods to quantitatively study the properties of phase-separate biomolecular condensates in diverse systems. The chapters in this book cover topics such as theoretical and computational methods; methods for in vitro characterization of biomolecular condensates; and techniques that enable in-cell characterization of biomolecular condensates. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Phase-Separated Biomolecular Condensates: Methods and Protocols is a valuable resource that helps researchers learn and use established methods to study both biophysical properties and biological functions of biomolecular condensates.

Author : James Whisstock,Phillip Bird
Publisher : Academic Press
Page : 562 pages
File Size : 50,5 Mb
Release : 2011-11-16
Category : Medical
ISBN : 9780123859501

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Serpin Structure and Evolution by James Whisstock,Phillip Bird Pdf

Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. This volume in the Methods in Enzymology series comprehensively covers this topic. With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems. This volume in the Methods in Enzymology series comprehensively covers the topic of serpins With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems

Author : Anonim
Publisher : Unknown
Page : 663 pages
File Size : 50,8 Mb
Release : 2010
Category : Electronic
ISBN : OCLC:672240143

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Single Molecule Tools by Anonim Pdf

Author : Jørgen Kjems,Elena Ferapontova,Kurt V. Gothelf
Publisher : Springer Science & Business Media
Page : 339 pages
File Size : 47,7 Mb
Release : 2013-10-19
Category : Science
ISBN : 9783642388156

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Nucleic Acid Nanotechnology by Jørgen Kjems,Elena Ferapontova,Kurt V. Gothelf Pdf

This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.

Author : Anonim
Publisher : Academic Press
Page : 362 pages
File Size : 43,7 Mb
Release : 2020-09-05
Category : Science
ISBN : 9780128211502

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Enzyme Engineering and Evolution: General Methods by Anonim Pdf

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series