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Author : Anonim
Publisher : Unknown
Page : 467 pages
File Size : 43,9 Mb
Release : 2017
Category : Electronic
ISBN : OCLC:1052766361
Author : Anonim
Publisher : Academic Press
Page : 484 pages
File Size : 51,7 Mb
Release : 2017-01-03
Category : Science
ISBN : 9780128095034
Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion
Author : Anonim
Publisher : Academic Press
Page : 616 pages
File Size : 55,8 Mb
Release : 2016-10-28
Category : Medical
ISBN : 9780128095478
Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion
Author : Arne Gennerich
Publisher : Springer Nature
Page : 753 pages
File Size : 45,7 Mb
Release : 2022-09-05
Category : Science
ISBN : 9781071622292
This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.
Author : Gregory I. Mashanov,Christopher Batters
Publisher : Humana Press
Page : 294 pages
File Size : 40,5 Mb
Release : 2011-08-04
Category : Science
ISBN : 1617792608
The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.
Author : Erwin J. G. Peterman,Gijs J. L. Wuite
Publisher : Humana Press
Page : 317 pages
File Size : 45,5 Mb
Release : 2011-09-15
Category : Science
ISBN : 1617792810
Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.
Author : Paul R. Selvin,Taekjip Ha
Publisher : CSHL Press
Page : 511 pages
File Size : 41,7 Mb
Release : 2008
Category : Atomic force microscopy
ISBN : 9780879697754
Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.
Author : Yakov M Tseytlin
Publisher : Academic Press
Page : 316 pages
File Size : 47,7 Mb
Release : 2016-07-29
Category : Science
ISBN : 9780128020364
Advanced Mechanical Models of DNA Elasticity includes coverage on 17 different DNA models and the role of elasticity in biological functions with extensive references. The novel advanced helicoidal model described reflects the direct connection between the molecule helix structure and its specific properties, including nonlinear features and transitions. It provides an introduction to the state of the field of DNA mechanics, known and widely used models with their short analysis, as well as coverage on experimental methods and data, the influence of electrical, magnetic, ionic conditions on the persistence length, and dynamics with viscosity influence. It then addresses the need to understand the nature of the non-linear overstretching transition of DNA under force and why DNA has a negative twist-stretch coupling. Includes coverage of 17 contemporary models of DNA mechanics with analysis Provides comparison of DNA and RNA mechanical features Covers advances in experimental techniques including AFM, X-ray, and optical tweezers Contains extensive references for further reading
Author : Peter Hinterdorfer,Antoine van Oijen
Publisher : Springer Science & Business Media
Page : 634 pages
File Size : 48,8 Mb
Release : 2009-12-24
Category : Science
ISBN : 9780387764979
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Author : Anonim
Publisher : Elsevier
Page : 374 pages
File Size : 46,7 Mb
Release : 2024-03-14
Category : Science
ISBN : 9780443222306
Magnetic Tweezers for the Study of Protein Structure and Function, Volume 694 in the Methods in Enzymology serial highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Single membrane protein tethering for magnetic tweezer experiments, Membrane protein folding studies using a robust magnetic tweezer method, Magnetic tweezers in cell mechanics, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology serials Updated release includes the latest information on Magnetic Tweezers for the Study of Protein Structure and Function
Author : R. Rigler,M. Orrit,T. Basche
Publisher : Springer Science & Business Media
Page : 375 pages
File Size : 51,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642565441
The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.
Author : Guanghua Wu
Publisher : Unknown
Page : 528 pages
File Size : 47,8 Mb
Release : 2001
Category : Electronic
ISBN : UCAL:C3480026
Author : Mustapha Hamdi,Antoine Ferreira
Publisher : Springer Science & Business Media
Page : 178 pages
File Size : 49,5 Mb
Release : 2010-10-06
Category : Technology & Engineering
ISBN : 9789048131808
Nanorobots represent a nanoscale device where proteins such as DNA, carbon nanotubes could act as motors, mechanical joints, transmission elements, or sensors. When these different components were assembled together they can form nanorobots with multi-degree-of-freedom, able to apply forces and manipulate objects in the nanoscale world. Design, Modeling and Characterization of Bio-Nanorobotic Systems investigates the design, assembly, simulation, and prototyping of biological and artificial molecular structures with the goal of implementing their internal nanoscale movements within nanorobotic systems in an optimized manner.
Author : Radislav A. Potyrailo,Vladimir M. Mirsky
Publisher : Springer Science & Business Media
Page : 495 pages
File Size : 41,6 Mb
Release : 2009-03-21
Category : Science
ISBN : 9780387737133
Chemical sensors are in high demand for applications as varied as water pollution detection, medical diagnostics, and battlefield air analysis. Designing the next generation of sensors requires an interdisciplinary approach. The book provides a critical analysis of new opportunities in sensor materials research that have been opened up with the use of combinatorial and high-throughput technologies, with emphasis on experimental techniques. For a view of component selection with a more computational perspective, readers may refer to the complementary volume of Integrated Analytical Systems edited by M. Ryan et al., entitled “Computational Methods for Sensor Material Selection”.
Author : Tadashi Nakano,Andrew W. Eckford,Tokuko Haraguchi
Publisher : Cambridge University Press
Page : 193 pages
File Size : 48,7 Mb
Release : 2013-09-12
Category : Technology & Engineering
ISBN : 9781107292383
This comprehensive guide, by pioneers in the field, brings together, for the first time, everything a new researcher, graduate student or industry practitioner needs to get started in molecular communication. Written with accessibility in mind, it requires little background knowledge, and provides a detailed introduction to the relevant aspects of biology and information theory, as well as coverage of practical systems. The authors start by describing biological nanomachines, the basics of biological molecular communication and the microorganisms that use it. They then proceed to engineered molecular communication and the molecular communication paradigm, with mathematical models of various types of molecular communication and a description of the information and communication theory of molecular communication. Finally, the practical aspects of designing molecular communication systems are presented, including a review of the key applications. Ideal for engineers and biologists looking to get up to speed on the current practice in this growing field.
