Software Assisted Method Development In High Performance Liquid Chromatography

Author : Szabolcs Fekete,Imre Molnar
Publisher : World Scientific
Page : 364 pages
File Size : 55,6 Mb
Release : 2018-08-01
Category : Science
ISBN : 9781786345479

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Software-assisted Method Development In High Performance Liquid Chromatography by Szabolcs Fekete,Imre Molnar Pdf

'The book is a useful contribution in the field of HPLC, and may represent a valuable tool for chromatography practitioners in different fields, as well as teachers and instructors. The 12 chapters provide comprehensive insights of current day retention and resolution modelling in HPLC, and its applications for small and large molecule analysis. It may be a useful reference for specialists in pharmaceuticals but not limited to … It may be a valuable resource to assist scientists involved in method development, aiming to achieve the best results with reduced costs, time, and efforts.'Analytical and Bioanalytical ChemistryThis handbook gives a general overview of the possibilities in recent developments in chromatographic retention modeling. As a result of the latest developments in modeling software, several new features are now accessible, opening a new level in HPLC method development.Many of these current possibilities in software assisted liquid chromatographic method modeling for analytical purposes are presented. Several modes of chromatography, including Reversed-Phase Liquid Chromatography (RPLC), Ion Exchange Chromatography (IEX), Hydrophobic Interaction Chromatography (HIC), and Hydrophilic Interaction Liquid Chromatography (HILIC) are explained in detail. For all these chromatographic modes, the most important variables for tuning retention and selectivity are exposed.Beside the industrial and practical benefits of retention modeling, the possibilities in teaching and education are also illustrated. Finally, numerous representative industrial examples are shown, to highlight the benefits, time and cost savings offered by state-of-the-art software assisted HPLC method development.

Author : Joseph L. Glajch,Lloyd R. Snyder
Publisher : Elsevier Publishing Company
Page : 714 pages
File Size : 46,5 Mb
Release : 1990
Category : Science
ISBN : UOM:39015021866408

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Computer-assisted Method Development for High-performance Liquid Chromatography by Joseph L. Glajch,Lloyd R. Snyder Pdf

This book deals with the use of the computer as an aid in selecting adequate or optimum conditions for a given analytical separation. Originally published as Volume 485 of the Journal of Chromatography, it has now been reprinted in book form, since the information is so useful that many chromatographers want a copy readily available in the lab. An extensive Introduction is added to the book edition. This surveys the field and refers to the pages where particular items are discussed in the book. The addition of a Glossary of Terms, an Author Index and a Subject Index make this book an invaluable source of easily consulted information for the practising chromatographer. For the purpose of this book, computer-assisted method development will be limited to specific procedures which are intended to be used with a computer - rather than their manually applied precursors. In that sense, the subject can be considered to have begun around 1980. The ongoing, intense research activity into various forms of computer assisted HPLC method development provides the assurance that this approach can really assist the practical chromatographer working in an industrial laboratory.

Author : Michael E. Swartz,Ira S. Krull
Publisher : CRC Press
Page : 95 pages
File Size : 41,7 Mb
Release : 2018-10-03
Category : Science
ISBN : 9781482229776

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Analytical Method Development and Validation by Michael E. Swartz,Ira S. Krull Pdf

Describes analytical methods development, optimization and validation, and provides examples of successful methods development and validation in high-performance liquid chromatography (HPLC) areas. The text presents an overview of Food and Drug Administration (FDA)/International Conference on Harmonization (ICH) regulatory guidelines, compliance with validation requirements for regulatory agencies, and methods validation criteria stipulated by the US Pharmacopia, FDA and ICH.

Author : Gregory K. Webster,Laila Kott
Publisher : CRC Press
Page : 567 pages
File Size : 48,9 Mb
Release : 2019-10-28
Category : Medical
ISBN : 9780429510397

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Chromatographic Methods Development by Gregory K. Webster,Laila Kott Pdf

This book is a comprehensive compilation of modern and cutting-edge chromatographic techniques written by pharmaceutical industry experts, academics, and vendors in the field. This book is an inclusive guide to developing all chromatographic methods (such as liquid chromatography and gas chromatography). It covers modern techniques for developing methods using chromatographic development software, requirements for validations, discussion on orthogonality, and how to transfer methods from HPLC to UHPLC. The text introduces some newer techniques that are heavily employed by chemists analyzing proteins and RNAi, as well as novel techniques such as counter current chromatography. This book is valuable for both the novice starting out in undergraduate labs and those who are new to the pharmaceutical industry and is a useful reference for seasoned analysts.

Author : Lloyd R. Snyder,Joseph J. Kirkland,Joseph L. Glajch
Publisher : John Wiley & Sons
Page : 665 pages
File Size : 52,8 Mb
Release : 2012-12-03
Category : Science
ISBN : 9781118591512

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Practical HPLC Method Development by Lloyd R. Snyder,Joseph J. Kirkland,Joseph L. Glajch Pdf

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Author : Lloyd R. Snyder,Joseph L. Glajch,Joseph Jack Kirkland
Publisher : Wiley-Interscience
Page : 296 pages
File Size : 45,6 Mb
Release : 1988
Category : High performance liquid chromatography
ISBN : UCSD:31822002498277

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Practical HPLC Method Development by Lloyd R. Snyder,Joseph L. Glajch,Joseph Jack Kirkland Pdf

Designed for the professional chemist who is already familiar with the basics of high performance liquid chromatography, this book explains how to develop separation methods for a variety of situations. It focuses on reversed-phase separations of small molecules and development of separations.

Author : Lloyd R. Snyder,John W. Dolan
Publisher : John Wiley & Sons
Page : 496 pages
File Size : 44,5 Mb
Release : 2007-01-09
Category : Science
ISBN : 9780470055519

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High-Performance Gradient Elution by Lloyd R. Snyder,John W. Dolan Pdf

Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution: * Provides specific, step-by-step recommendations for developing a gradient separation for any sample * Describes the best approach for troubleshooting problems with gradient methods * Guides the reader on the equipment used for gradient elution * Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients * Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.

Author : Monika Waksmundzka-Hajnos,Joseph Sherma
Publisher : CRC Press
Page : 996 pages
File Size : 44,8 Mb
Release : 2010-11-08
Category : Health & Fitness
ISBN : 1420092618

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High Performance Liquid Chromatography in Phytochemical Analysis by Monika Waksmundzka-Hajnos,Joseph Sherma Pdf

The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp

Author : Tomasz Tuzimski,Joseph Sherma
Publisher : CRC Press
Page : 583 pages
File Size : 42,7 Mb
Release : 2015-05-20
Category : Science
ISBN : 9781466568822

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High Performance Liquid Chromatography in Pesticide Residue Analysis by Tomasz Tuzimski,Joseph Sherma Pdf

HPLC is the principal separation technique for identification of the pesticides in environmental samples and for quantitative analysis of analytes. At each stage of the HPLC procedure, the chromatographer should possess both the practical and theoretical skills required to perform HPLC experiments correctly and to obtain reliable, repeatable, and r

Author : Stavros Kromidas
Publisher : John Wiley & Sons
Page : 416 pages
File Size : 55,7 Mb
Release : 2021-08-06
Category : Science
ISBN : 9783527828517

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Optimization in HPLC by Stavros Kromidas Pdf

Learn to maximize the performance of your HPLC or UHPLC system with this resource from leading experts in the field Optimization in HPLC: Concepts and Strategies delivers tried-and-tested strategies for optimizing the performance of HPLC and UHPLC systems for a wide variety of analytical tasks. The book explains how to optimize the different HPLC operation modes for a range of analyses, including small molecules, chiral substances, and biomolecules. It also shows readers when and how computational tools may be used to optimize performance. The practice-oriented text describes common challenges faced by users and developers of HPLC and UHPLC systems, as well as how those challenges can be overcome. Written for first-time and experienced users of HPLC technology and keeping pace with recent developments in HPLC instrumentation and operation modes, this comprehensive guide leaves few questions unanswered. Readers will also benefit from the inclusion of: A thorough introduction to optimization strategies for different modes and uses of HPLC, including working under regulatory constraints An exploration of computer aided HPLC optimization, including ChromSwordAuto and Fusion QbD A treatment of current challenges for HPLC users in industry as well as large and small analytical service providers Discussions of current challenges for HPLC equipment suppliers Tailor-made for analytical chemists, chromatographers, pharmacologists, toxicologists, and lab technicians, Optimization in HPLC: Concepts and Strategies will also earn a place on the shelves of analytical laboratories in academia and industry who seek a one-stop reference for optimizing the performance of HPLC systems.

Author : Stavros Kromidas
Publisher : John Wiley & Sons
Page : 418 pages
File Size : 42,5 Mb
Release : 2021-11-08
Category : Science
ISBN : 9783527347896

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Optimization in HPLC by Stavros Kromidas Pdf

Learn to maximize the performance of your HPLC or UHPLC system with this resource from leading experts in the field Optimization in HPLC: Concepts and Strategies delivers tried-and-tested strategies for optimizing the performance of HPLC and UHPLC systems for a wide variety of analytical tasks. The book explains how to optimize the different HPLC operation modes for a range of analyses, including small molecules, chiral substances, and biomolecules. It also shows readers when and how computational tools may be used to optimize performance. The practice-oriented text describes common challenges faced by users and developers of HPLC and UHPLC systems, as well as how those challenges can be overcome. Written for first-time and experienced users of HPLC technology and keeping pace with recent developments in HPLC instrumentation and operation modes, this comprehensive guide leaves few questions unanswered. Readers will also benefit from the inclusion of: A thorough introduction to optimization strategies for different modes and uses of HPLC, including working under regulatory constraints An exploration of computer aided HPLC optimization, including ChromSwordAuto and Fusion QbD A treatment of current challenges for HPLC users in industry as well as large and small analytical service providers Discussions of current challenges for HPLC equipment suppliers Tailor-made for analytical chemists, chromatographers, pharmacologists, toxicologists, and lab technicians, Optimization in HPLC: Concepts and Strategies will also earn a place on the shelves of analytical laboratories in academia and industry who seek a one-stop reference for optimizing the performance of HPLC systems.

Author : S. Pryde,M.T. Gilbert
Publisher : Springer Science & Business Media
Page : 288 pages
File Size : 41,5 Mb
Release : 1979-01-31
Category : Science
ISBN : 0412142201

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Applications of High Performance Liquid Chromatography by S. Pryde,M.T. Gilbert Pdf

Author : Afiya Ansari
Publisher : Createspace Independent Publishing Platform
Page : 50 pages
File Size : 55,5 Mb
Release : 2017-02-14
Category : Electronic
ISBN : 1543104355

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Method Development,validation & Types of High Performance Liquid Chromatography by Afiya Ansari Pdf

Chromatography may be defined as a nondestructive procedure for separating mixture of components into individual components through equilibrium distribution between two phases. The technique of chromatography is based on the differences in the rate at which the components of a mixture move through a porous medium under the influence of solvent or gas. The importance of Chromatography is increasing rapidly in pharmaceutical analysis in exact differentiation, selective identification and quantitative determination of structurally closely related compounds. Another important field of application of chromatographic methods is the purity testing of final products and intermediates (detection of decomposition products and by-products). As a consequence of the above points, chromatographic methods are occupying an ever-expanding position in the latest editions of the pharmacopoeias and other testing standards. Chromatography is one of the most powerful and versatile analytical techniques available to the modern chemist. Its power arises from its capacity to determine quantitatively many individual components present in a mixture in a one single analytical run. Its versatility comes from its capacity to handle wide variety of samples that may be gaseous, liquid or solid in nature. The sample can range in complexity from a single substance to a multicomponent mixture containing widely different chemical species. Another aspect of versatility is that the analysis can be carried out on a very costly complex instrument and on the other hand on a simple inexpensive thin layer plate. The modern form of column chromatography has been called high performance, high pressure, High resolution and high speed liquid chromatography. High Performance Liquid Chromatography (HPLC) is a special branch of column chromatography in which the mobile phase is forced through the column at high speed. As a result the analysis time is reduced by 1-2 orders of magnitude relative to classical column chromatography and the use of much smaller particles of the adsorbent or support becomes possible increasing the column efficiency substantially.

Author : Csaba Horváth
Publisher : Elsevier
Page : 342 pages
File Size : 51,7 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483262024

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High-Performance Liquid Chromatography by Csaba Horváth Pdf

High-Performance Liquid Chromatography: Advances and Perspectives, Volume 1 deals with the fundamental aspects of high-performance liquid chromatography, a technique used in chemical analysis. The publication provides accounts, presented by experts in the field, of a variety of topics in high-performance liquid chromatography. Each chapter covers interesting subjects such as the evolution of liquid chromatography; the use of bonded phases in high-performance chromatography; effects of ionization and complex formation on retention and selectivity in reversed-phase chromatography; and gradient elution. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a valuable reference material.

Author : Eli Grushka,Nelu Grinberg
Publisher : CRC Press
Page : 270 pages
File Size : 55,6 Mb
Release : 2016-11-03
Category : Science
ISBN : 9781315353319

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Advances in Chromatography, Volume 53 by Eli Grushka,Nelu Grinberg Pdf

For more than four decades, scientists and researchers have relied on the Advances in Chromatography series for the most up-to-date information on a wide range of developments in chromatographic methods and applications. For Volume 53, the series editors have invited established, well-known chemists to offer cutting-edge reviews of chromatographic methods with applications in the life sciences. The clear presentation of topics and vivid illustrations for which this series has become known makes the material accessible and engaging to analytical, biochemical, organic, polymer, and pharmaceutical chemists at all levels of technical skill.